The Omicron variant is a new variant of the Coronavirus which is thought to be able to spread and infect humans faster than the previous variant. The rapid transmission of the disease and the high mortality cause great panic and anxiety nr the general public and the health sector. One of the health workers who are very vulnerable to receiving the impact of the emergence of COVID-19 is nurses. In addition to being directly infected, psychological disorders such as anxiety, stress, and depression are very easily experienced by nurses due to various causes. This study aimed to know the determinants of anxiety, stress, and depression during the COVID-19 pandemic of the Omicron variant. The research method is a quantitative study with a cross-sectional approach. This research was conducted in the adult inpatient room at the RSUD Kota Bandung in February 2022. The respondents were 64 nurses with a total sampling technique. The research instrument used a Depression Anxiety Stress Scale-42 (DASS-42) questionnaire, which was collected via a google form. The data were analyzed using Pearson chi-square test. The results showed that a small proportion of nurses experience mild to moderate anxiety, stress, and depression. The bivariate test showed no significant relationship between age, gender, education, and length of work with anxiety, stress, and depression (p0.05) in nurses in the adult inpatient room at the RSUD Kota Bandung. Although small, there are still nurses who experience stress, anxiety, and depression, so the hospital should provide training on how to deal with anxiety, stress, and depression for nurses to have adaptive coping mechanisms when anxiety, stress, or depression occurs.