Accurate and rapid fixation of key parts of sika deer is essential for the mechanized and intelligent collection of both deer blood and velvet antler. To solve the low detection accuracy problem of key parts of deer caused by the diversity of deer farm breeding environment and the rapid movement of deer, this paper proposes an AD-YOLOv5 algorithm for detecting key parts of sika deer based on YOLOv5s. Specifically, a new weighted bidirectional feature pyramid network S-BiFPN is first proposed, which both simplifies the structure and effectively performs skip feature fusion to better capture object information at different scales, so that the adaptive feature fusion capability of the model is optimized. Second, this paper proposes to add a SENet module after the C3 module of the 9th Layer in the backbone part of the network, so that the network can adaptively readjust the channel weights of feature maps to enhance the important features for key parts of sika deer and improve the expression ability of the model for key parts. Furthermore, to make the model more stable in the training process, a novel bounding box regression loss function SIoU is led into, which can better learn the position and size of the bounding box by fusing the orientation information between the ground truth box and the predicted box. The effectiveness of the proposed algorithm is demonstrated through ablation experiments. Experimental results show that the mAP@0.5 of the proposed algorithm reaches 97.30 % for the detection of key parts of sika deer, which is 12.85 %, 9.65 %, 9.18 %, 7.13 %, 3.59 %, 4.6 %, 0.90 % and 0.10 % higher than SSD, EfficientDet, YOLOv3, YOLOv4, Faster R-CNN, YOLOv5s, YOLOv7 and YOLOv8s, respectively. This research provides a new idea for detecting key parts of animals in complex environments and lays a theoretical foundation for the development of intelligent fixation devices for sika deer.