The purpose of this paper is threefold. First, we prove sharp singular affine Moser–Trudinger inequalities on both bounded and unbounded domains in \({\mathbb {R}}^{n}\). In particular, we will prove the following much sharper affine Moser–Trudinger inequality in the spirit of Lions (Rev Mat Iberoamericana 1(2):45–121, 1985) (see our Theorem 1.4): Let \(\alpha _{n}=n\left( \frac{n\pi ^{\frac{n}{2}}}{\Gamma (\frac{n}{2}+1)}\right) ^{\frac{1}{n-1}}\), \(0\le \beta 0\). Then there exists a constant \(C=C\left( n,\beta \right) >0\) such that for all \(0\le \alpha \le \left( 1-\frac{\beta }{n}\right) \alpha _{n}\) and \(u\in C_{0}^{\infty }\left( {\mathbb {R}}^{n}\right) \setminus \left\{ 0\right\} \) with the affine energy \(~{\mathcal {E}}_{n}\left( u\right) <1\), we have $$\begin{aligned} {\displaystyle \int \nolimits _{{\mathbb {R}}^{n}}} \frac{\phi _{n,1}\left( \frac{2^{\frac{1}{n-1}}\alpha }{\left( 1+{\mathcal {E}}_{n}\left( u\right) ^{n}\right) ^{\frac{1}{n-1}}}\left| u\right| ^{\frac{n}{n-1}}\right) }{\left| x\right| ^{\beta }}dx\le C\left( n,\beta \right) \frac{\left\| u\right\| _{n}^{n-\beta }}{\left| 1-{\mathcal {E}}_{n}\left( u\right) ^{n}\right| ^{1-\frac{\beta }{n}}}. \end{aligned}$$ Moreover, the constant \(\left( 1-\frac{\beta }{n}\right) \alpha _{n}\) is the best possible in the sense that there is no uniform constant \(C(n, \beta )\) independent of u in the above inequality when \(\alpha >\left( 1-\frac{\beta }{n}\right) \alpha _{n}\). Second, we establish the following improved Adams type inequality in the spirit of Lions (Theorem 1.8): Let \(0\le \beta 0\). Then there exists a constant \(C=C\left( m,\beta ,\tau \right) >0\) such that $$\begin{aligned} \underset{u\in W^{2,m}\left( {\mathbb {R}}^{2m}\right) , \int _{ {\mathbb {R}}^{2m}}\left| \Delta u\right| ^{m}+\tau \left| u\right| ^{m} \le 1}{\sup } {\displaystyle \int \nolimits _{{\mathbb {R}}^{2m}}} \frac{\phi _{2m,2}\left( \frac{2^{\frac{1}{m-1}}\alpha }{\left( 1+\left\| \Delta u\right\| _{m}^{m}\right) ^{\frac{1}{m-1}}}\left| u\right| ^{\frac{m}{m-1}}\right) }{\left| x\right| ^{\beta }}dx\le C\left( m,\beta ,\tau \right) , \end{aligned}$$ for all \(0\le \alpha \le \left( 1-\frac{\beta }{2m}\right) \beta (2m,2)\). When \(\alpha >\left( 1-\frac{\beta }{2m}\right) \beta (2m,2)\), the supremum is infinite. In the above, we use $$\begin{aligned} \phi _{p,q}(t)=e^{t}- {\displaystyle \sum \limits _{j=0}^{j_{\frac{p}{q}}-2}} \frac{t^{j}}{j!},\,\,\,j_{\frac{p}{q}}=\min \left\{ j\in {\mathbb {N}} :j\ge \frac{p}{q}\right\} \ge \frac{p}{q}. \end{aligned}$$ The main difficulties of proving the above results are that the symmetrization method does not work. Therefore, our main ideas are to develop a rearrangement-free argument in the spirit of Lam and Lu (J Differ Equ 255(3):298–325, 2013; Adv Math 231(6): 3259–3287, 2012), Lam et al. (Nonlinear Anal 95: 77–92, 2014) to establish such theorems. Third, as an application, we will study the existence of weak solutions to the biharmonic equation $$\begin{aligned} \left\{ \begin{array}{l} \Delta ^{2}u+V(x)u=f(x,u)\text { in }{\mathbb {R}}^{4}\\ u\in H^{2}\left( {\mathbb {R}}^{4}\right) ,~u\ge 0 \end{array} \right. , \end{aligned}$$ where the nonlinearity f has the critical exponential growth.