Acute burn care (>30% TBSA) requires IV resuscitation with large volumes of fluids. Lactated Ringer’s (LR) is the most commonly utilized fluid however, other crystalloids (PlasmaLyte; PL) and colloids (fresh frozen plasma [FFP]; 5% Albumin [ALB]) have been suggested. While resuscitation maintains hemodynamics, limited volumes may suffice in resource-poor environments or mass casualty scenarios. This study compares limited IV resuscitation to the modified Brooke (MB) formula, with emphasis on liver function. Thirty anesthetized swine were subjected to 40% TBSA contact burns and randomized to one of five groups (n=6/group): 15mL/kg/d with LR; PL; FFP; ALB; was compared to 2ml/kg/%TBSA LR (MB). Animals recovered in metabolic cages for urine and blood collection (baseline (BL), 6, 12, 24, 32, and 48h post-burn) to quantify biochemical markers. Following euthanasia (48h) liver tissue samples were snap frozen for analysis. Four non-thermally injured animals (sham) served as tissue controls. Plasma protein was elevated at 48h with colloids (p≤0.002) and albumin levels were elevated throughout the study in the ALB group (p<0.0001). When compared with BL levels, liver enzymes alanine (68.3±1.3 vs 84.0±2.9 mg/dL; p<0.0001) and aspartate (31.0±1.0 vs 90.2±4.3 mg/dL; p<0.0001) aminotransferases were elevated by 6h post burn and remained high regardless of treatment. In the ALB group levels of bilirubin (p<0.0001) were higher throughout the course of the experiment when compared with all other treatments. Total urine output was greater in animals receiving MB (1703±245 mL) when compared with LR (1050±61 mL; p=0.07), PL (797±160 mL; p=0.007), and ALB (933±160 mL; p=0.09), but not FFP (1330±181 mL; p=0.61). Caspase was significantly up regulated in MB, LR, PL, and ALB when compared with sham animals (p≤ 0.019). Heat shock protein 70 tended to be greater in MB and L-LR than sham animals (p≤0.066). Histology revealed reduced intracellular glycogen reduced due to burn (p≤0.01) and a significantly higher level of edema in ALB when compared with other treatment groups (p≤0.16) as demonstrated by histology. Results from these experiments suggest that in this model, resuscitation with ALB early post-burn negatively impacts liver function. No abnormal changes in liver damage markers were detected in animals receiving FFP. Data from these studies can help inform limited volume resuscitative efforts for patients with burns. Limited IV fluid resuscitation is common in resource poor environments. Any available IV fluid would be effective to buy time in prolonged field care, however physicians should monitor liver function if using ALB.
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