Hybrid vehicles account for the largest share of new motor vehicle sales in Europe. These are vehicles that are expected to bridge the technological gap between vehicles with internal combustion engines and electric vehicles. Such a solution also makes it possible to meet the limits of motor vehicle emissions, at a time when it is particularly important to test them under actual traffic conditions. This article analyzes the impact of the length of the test routes in relation to current, but also future regulations of approval standards. Three routes of post-phase composition (urban, rural, motorway) with lengths of about 30, 16 and 8 km were selected for the study. Measurements of the main emission components were made using portable emission measurement systems (PEMS), and exhaust emissions were determined using the moving average window (MAW) method. Analysis of the obtained results led to the conclusion that the current requirements for the RDE test (in particular, the duration of the test) enforce a length of each part of 32 km. Reducing the test to 60–90 min causes the individual phases to last 16 km, and the main advantage of such a solution is the very strong influence of the cold start phase on the emission results in the urban phase. Future declarations by lawmakers to drastically reduce the length of the test phases to 8 km will force hybrid vehicles to be tested largely using the internal combustion engine. This will be the right thing to do, especially in the urban phase, as now in addition to a significant reduction in the engine warm-up phase, manufacturers will have to take into account that such an engine thermal condition can also occur in the rural phase.