Commercial farming of endangered wildlife has potential to reduce poaching pressure on wild populations. However, poached products can be laundered as farmed products. A method for separating farmed from wild products is therefore essential for effective law enforcement. Meat (skeletal muscle) is a wildlife product whose origin cannot be correctly determined. This short communication reports an epigenetic approach to achieve the distinction using the methylation rate of the promoter of the α-actinin-3 (ACTN3) gene in gluteus maximus tissues of feral (n = 21) and farmed (n = 21) American mink, Neovison vison. Our results showed that the accuracy of assignment ranged from 59.5 to 76.2% on each of six CpG sites/site groups. Combination of the six CpG sites/site groups achieved 83.3% overall correct assignment, 90.5% for the farmed group and 76.2% for the wild group. These data suggest the methylation rate of promoters of selected genes could be an effective indicator to distinguish farmed meat from wild meat of wildlife species.