Onset Of Activity Research Articles

Cortical activation in REM behavior disorder mimics voluntary movement. An electroencephalography study

ObjectivesMotor symptoms of Parkinson’s disease improve during REM sleep behavior disorder movement episodes. Our aim was to study cortical activity during these movement episodes, in patients with and without Parkinson’s disease, in order to investigate the cortical involvement in the generation of its electromyographic activity and its potential relationship with Parkinson’s disease. MethodsWe looked retrospectively in our polysomnography database for patients with REM sleep behavior disorder, analyzing fifteen patients in total, seven with idiopathic REM sleep behavior disorder and eight associated with Parkinson’s disease. We selected segments of REM sleep with the presence of movements (evidenced by electromyographic activation), and studied movement-related changes in cortical activity by averaging the electroencephalographic signal (premotor potential) and by means of time/frequency transforms. ResultsWe found a premotor potential and an energy decrease of alpha–beta oscillatory activity preceding the onset of electromyographic activity, together with an increase of gamma activity for the duration of the movement. All these changes were similarly present in REM sleep behavior disorder patients with and without Parkinson’s disease. ConclusionsMovement-related changes in electroencephalographic activity observed in REM sleep behavior disorder are similar to those observed during voluntary movements, regardless of the presence of Parkinson’s disease motor symptoms. SignificanceThese results suggest a main involvement of the cortex in the generation of the movements during REM sleep.

Relative uteroplacental insufficiency of labor.

Relative uteroplacental insufficiency of labor (RUPI-L) is a clinical condition that refers to alterations in the fetal oxygen "demand-supply" equation caused by the onset of regular uterine activity. The term RUPI-L indicates a condition of "relative" uteroplacental insufficiency which is relative to a specific stressful circumstance, such as the onset of regular uterine activity. RUPI-L may be more prevalent in fetuses in which the ratio between the fetal oxygen supply and demand is already slightly reduced, such as in cases of subclinical placental insufficiency, post-term pregnancies, gestational diabetes, and other similar conditions. Prior to the onset of regular uterine activity, fetuses with a RUPI-L may present with normal features on the cardiotocography. However, with the onset of uterine contractions, these fetuses start to manifest abnormal fetal heart rate patterns which reflect the attempt to maintain adequate perfusion to essential central organs during episodes of transient reduction in oxygenation. If labor is allowed to continue without an appropriate intervention, progressively more frequent, and stronger uterine contractions may result in a rapid deterioration of the fetal oxygenation leading to hypoxia and acidosis. In this Commentary, we introduce the term relative uteroplacental insufficiency of labor and highlight the pathophysiology, as well as the common features observed in the fetal heart rate tracing and clinical implications.

Comparative speed of kill provided by lotilaner (Credelio™), sarolaner (Simparica Trio™), and afoxolaner (NexGard™) to control Amblyomma americanum infestations on dogs

BackgroundCanine acaricides with rapid onset and sustained activity can reduce pathogen transmission risk and enhance pet owner experience. This randomized, complete block design, investigator-masked study compared the speed of kill of Amblyomma americanum provided by three monthly-use isoxazoline-containing products.MethodsEight randomized beagles per group were treated (day 0), per label, with sarolaner (combined with moxidectin and pyrantel, Simparica Trio™), afoxolaner (NexGard™), or lotilaner (Credelio™), or remained untreated. Infestations with 50 adult A. americanum were conducted on days − 7, − 2, 21, and 28, and tick counts were performed on day − 5 (for blocking), and at 4, 8, 12, 24, 48, and 72 h following treatment and subsequent infestations. Efficacy calculations were based on geometric mean live tick counts. A linear mixed model was used for between-group comparisons.ResultsOn day 0, only lotilaner significantly reduced an A. americanum infestation by 12 h (43.3%; P = 0.002). Efficacy of lotilaner and afoxolaner at 24 h post-treatment was 95.3% and 97.6%, respectively, both significantly different from sarolaner (74%) (P = 0.002, P < 0.001, respectively). On day 21, at 12 h postinfestation, lotilaner efficacy (59.6%) was significantly different from sarolaner (0.0%) (P < 0.001) and afoxolaner (6.3%) (P < 0.001). At 24 h, lotilaner efficacy (97.4%) was significantly different (P < 0.001) from sarolaner and afoxolaner (13.6% and 14.9%, respectively). On day 28, at 12 h postinfestation, lotilaner efficacy (47.8%) was significantly different from sarolaner (17.1%) (P = 0.020) and afoxolaner (9.0%) (P = 0.006). At 24 h, lotilaner efficacy (92.3%) was significantly different from sarolaner 4.9% (P < 0.001) and afoxolaner (0.0%) (P < 0.001). Speed of kill for sarolaner and afoxolaner, but not lotilaner, significantly declined over the study period. Following reinfestation on day 28, neither sarolaner nor afoxolaner reached 90% efficacy by 48 h. By 72 h, sarolaner efficacy was 97.4% and afoxolaner efficacy was 86.3%. Only lotilaner achieved ≥ 90% efficacy by 24 h post-treatment and 24 h postinfestation on days 21 and 28. Time to ≥ 90% efficacy following new infestations consistently occurred 24–48 h earlier for lotilaner compared with sarolaner or afoxolaner.ConclusionsCredelio (lotilaner) has a more rapid onset of acaricidal activity against A. americanum than Simparica Trio (sarolaner-moxidectin-pyrantel) and NexGard (afoxolaner). Only lotilaner’s speed of tick kill is sustained throughout the dosing period.Graphical

Muscle MRI as a biomarker of disease activity and progression in myotonic dystrophy type 1: a longitudinal study

IntroductionMyotonic dystrophy type 1 (DM1) is an autosomal dominant disease characterized by myotonia and progressive muscular weakness and atrophy. The aim of this study was to investigate the usefulness of longitudinal muscle MRI in detecting disease activity and progression in DM1, and to better characterize muscle edema, fat replacement and atrophy overtime.Materials and methodsThis is a prospective, observational, longitudinal study including 25 DM1 patients that performed at least two muscle MRIs. Demographic and genetic characteristics were recorded. Muscular Impairment Rating Scale (MIRS) and MRC score were performed within 3 months from MRIs at baseline (BL) and at follow-up (FU). We analysed 32 muscles of lower body (LB) and 17 muscles of upper body (UB) by T1 and STIR sequences. T1-, STIR- and atrophy scores and their variations were evaluated. Correlations between MRIs’ scores and demographic, clinical and genetic characteristics were analysed.ResultsEighty (80%) of patients showed fat replacement progression at FU. The median T1 score progression (ΔT1-score) was 1.3% per year in LB and 0.5% per year in UB. The rate of fat replacement progression was not homogenous, stratifying patients from non-progressors to fast progressors (> 3% ΔT1-score per year). Half of the STIR-positive muscles at BL showed T1-score progression at FU. Two patients with normal MRI at baseline only showed STIR-positive muscle at FU, marking the disease activity onset. STIR positivity at baseline correlated with fat replacement progression (ΔT1-score; p < 0.0001) and clinical worsening at FU (ΔMRC-score; p < 0.0001). Sixty-five (65%) of patients showed STIR- and fat replacement-independent muscle atrophy progression, more evident in UB.ConclusionsMuscle MRI represents a sensitive biomarker of disease activity, severity, and progression in DM1. STIR alterations precede fat replacement and identify patients with a higher risk of disease progression, while T1-sequences reveal atrophy and fat replacement progression before clinical worsening.

Dopamine and deep brain stimulation accelerate the neural dynamics of volitional action in Parkinson's disease.

The ability to initiate volitional action is fundamental to human behaviour. Loss of dopaminergic neurons in Parkinson's disease is associated with impaired action initiation, also termed akinesia. Both dopamine and subthalamic deep brain stimulation (DBS) can alleviate akinesia, but the underlying mechanisms are unknown. An important question is whether dopamine and DBS facilitate de novo build-up of neural dynamics for motor execution or accelerate existing cortical movement initiation signals through shared modulatory circuit effects. Answering these questions can provide the foundation for new closed-loop neurotherapies with adaptive DBS, but the objectification of neural processing delays prior to performance of volitional action remains a significant challenge. To overcome this challenge, we studied readiness potentials and trained brain signal decoders on invasive neurophysiology signals in 25 DBS patients (12 female) with Parkinson's disease during performance of self-initiated movements. Combined sensorimotor cortex electrocorticography and subthalamic local field potential recordings were performed OFF therapy (n = 22), ON dopaminergic medication (n = 18) and on subthalamic deep brain stimulation (n = 8). This allowed us to compare their therapeutic effects on neural latencies between the earliest cortical representation of movement intention as decoded by linear discriminant analysis classifiers and onset of muscle activation recorded with electromyography. In the hypodopaminergic OFF state, we observed long latencies between motor intention and motor execution for readiness potentials and machine learning classifications. Both, dopamine and DBS significantly shortened these latencies, hinting towards a shared therapeutic mechanism for alleviation of akinesia. To investigate this further, we analysed directional cortico-subthalamic oscillatory communication with multivariate granger causality. Strikingly, we found that both therapies independently shifted cortico-subthalamic oscillatory information flow from antikinetic beta (13-35 Hz) to prokinetic theta (4-10 Hz) rhythms, which was correlated with latencies in motor execution. Our study reveals a shared brain network modulation pattern of dopamine and DBS that may underlie the acceleration of neural dynamics for augmentation of movement initiation in Parkinson's disease. Instead of producing or increasing preparatory brain signals, both therapies modulate oscillatory communication. These insights provide a link between the pathophysiology of akinesia and its' therapeutic alleviation with oscillatory network changes in other non-motor and motor domains, e.g. related to hyperkinesia or effort and reward perception. In the future, our study may inspire the development of clinical brain computer interfaces based on brain signal decoders to provide temporally precise support for action initiation in patients with brain disorders.

Aeolian activity in the Yarlung Zangbo River Basin, southern Tibetan Plateau, began at 584 ka: implications for the glaciation of the Tibetan Plateau

Aeolian activity on the Tibetan Plateau (TP) has profound effect on the Asian and global climate. In the southern TP, loess deposition widely occurred associated with the massive glaciers and large rivers, recording the interplay history among the aeolian activity, atmosphere, and cryosphere. However, the initiation age of the loess and the factor controlling the loess formation are still controversial. Here, three drilling cores were retrieved from Linzhi area, in the eastern Yarlung Zangbo River Basin of the southern TP, which reached the bedrock and documented the loess development in the southern TP. Based on the optically stimulated luminescence, electron spin resonance, paleomagnetism dating and relative paleointensity correlation, the basal age of the drilling core was constrained at 584 ka revealing the onset of significant aeolian activity on the southern TP. Synthesize this study and previous work on the loess age across the TP, three stages were identified, namely 3600, 2800–2700, and 1200–600 ka, with the age trending younger from north to south. Extensive loess development on the TP occurred between 1200 and 600 ka, which was mainly ascribed to the global cooling during the mid-Pleistocene Transition. During this time, the ampler glaciers extent significantly expanded, westerly displaced southward and the wind intensity also intensified on the TP, which resulted in the enhancement of dust generation, arid and windy condition, leading to the loess preservation and development on the TP. Moreover, numerous evidences suggested that the glaciers on the TP may significantly developed since 800 ka, then enlarged at 500–600 ka, which may lead to the massive dust/loess input to the TP, Chinese Loess Plateau and north Pacific Ocean.

Predictions for Sparse Photometry of Jupiter-family Comet Nuclei in the LSST Era

The Legacy Survey of Space and Time (LSST) at Vera C. Rubin Observatory will deliver high-quality, temporally sparse observations of millions of solar system objects on an unprecedented scale. Such data sets will likely enable the precise estimation of small-body properties on a population-wide basis. In this work, we consider the possible applications of photometric data points from LSST to the characterization of Jupiter-family comet (JFC) nuclei. We simulate sparse-in-time lightcurve points with an LSST-like cadence for the orbit of a JFC between 2024 and 2033. Convex lightcurve inversion is used to assess whether the simulation input parameters can be accurately reproduced for a sample of nucleus rotation periods, pole orientations, activity onsets, shapes, and sizes. We find that the rotation period and pole direction can be reliably constrained across all nucleus variants tested, and that the convex shape models, while limited in their ability to describe complex or bilobed nuclei, are effective for correcting sparse photometry for rotational modulation to improve estimates of nucleus phase functions. Based on this analysis, we anticipate that LSST photometry will significantly enhance our present understanding of the spin state and phase function distributions of JFC nuclei.

WITHDRAWN: Reconstructing the environmental impact of mining on mountain lakes

Mountain lakes are particularly fragile ecosystems undergoing important transformations associated with ongoing global change. However, the history of anthropogenic impacts on mountain lakes and their catchments is much longer, in many cases featuring millennia of summer pastoral farming. More recently, the growing demand for raw materials and energy linked to industrialization, particularly accelerated since the 19th century CE, meant a further increase in human impact on mountain areas. The Cantabrian Range (northern Spain) constitutes a paradigmatic case of southern European mountain range experiencing intense human impact during the past few millennia and particularly the past two centuries. Here, we have reconstructed the environmental dynamics of this area during the last millennium, with a particular focus on the impact of mining, based on the multidisciplinary analysis (sedimentology, biogeochemistry, magnetic susceptibility, diatoms, pollen, charcoal and dung fungal spores) of sediment cores from Lago de La Cueva (43°03′N, 6°06′W, 1550 m a.s.l.). Changing land use and climate have driven lake dynamics during the last centuries. A major fire-caused deforestation event dated to the late 15th century CE increased erosion and the frequency of intense runoff episodes, in the context of the wetter and colder Little Ice Age. The onset of iron mining activities in the catchment ca. 200 years ago had a strong impact on the lake. Sedimentation rates notably raised and mining waste containing hematite and potentially toxic elements (e.g. Fe, Co, As) was washed into the lake. Additionally, diatom assemblages showed that lake regulation since the early 20th century CE severely altered the natural hydrological regime introducing rapid seasonal lake-level oscillations and increased lakeshore erosion, water turbidity and nutrient loads. The recent environmental restoration, finished in 2006, has involved the re-deposition of large volumes of mine tailing. Although some mining wastewater still arrives into the lake because mine drainage is still active, restoration works have succeeded in reducing erosion rates and nutrient loads. Lower land-use intensity has also contributed to natural vegetation recovery, further diminishing erosion. This study illustrates the complex interactions between human activities (grazing, mining, hydropower) and climate change in defining mountain landscape shifts through time. Moreover, our results highlight the usefulness of paleolimnological research to quantitatively assess the effectiveness of lake restoration programs.

Inelastic Neutron Scattering Studies of Propene and 1-Octene Oligomerisation in H-ZSM-5

Neutron scattering methods such as quasielastic neutron scattering (QENS) and inelastic neutron scattering (INS) have been used to study the reactivity of propene and 1-octene over the acid zeolite catalyst H-ZSM-5. The high activity of the catalyst causes the alkenes to form linear oligomers below room temperature. INS has shown that the reaction proceeds through a hydrogen-bonded intermediate. Studies using propane as an inert analogue for propene have found that the adsorbed C3 molecules spend much of their time undergoing short jumps within the pore channels of the zeolite. Hydrothermal dealumination plays an important role in determining the activity of zeolite catalysts. Dealumination was found to delay the onset of catalytic activity for oligomerisation to higher temperatures and increase the mobility of hydrocarbons within the zeolite, both due to reduced acid-hydrocarbon interactions.

Persistent Behavior in Solar Energetic Particle Time Series

We investigate the long-term persistence of solar energetic particle (SEP) time series by means of four different methods: Hurst rescaled range R/S analysis, detrended fluctuation analysis, centered moving average analysis, and the fluctuation of natural time under the time reversal method. For these analyses, we use data sets from the Integrated Science Investigation of the Sun instrument suite on board NASA's Parker Solar Probe. Background systematic noise is modeled using cross-correlation analysis between different SEP energy channels and subtracted from the original data. The use of these four methods for deriving the time-series persistence allows us to (i) differentiate between quiet- and active-Sun periods based on the values of the corresponding self-similarity exponents alone; (ii) identify the onset of an ongoing activity well before it reaches its maximum SEP flux; (iii) reveal an interesting fine structure when activity is observed; and (iv) provide, for the first time, an estimate of the maximum SEP flux of a future storm based on the entropy change of natural time under time reversal.

High resolution luminescence and radiocarbon dating of Holocene Aeolian silt (loess) in west Greenland

Loess–palaeosol sequences serve as valuable archives of changes in climate and atmospheric mineral dust deposition. However, little work has been conducted on Holocene loess in the Arctic, despite the sensitivity of this region to climate changes. Aeolian silt/loess profiles in the ice-free region of western Greenland near Kangerlussuaq were sampled to develop a robust age framework using both luminescence and bulk organic matter radiocarbon dating. Radiocarbon ages generally show consistent age increases with depth but are likely offset to younger ages due to sediment mixing in the upper 10–20 cm of the profiles. Quartz OSL signals exhibit insensitivity, while low-temperature infrared stimulated luminescence performed at 50 °C (IR50) and the post-IR IRSL at 180 °C (pIRIR180) signals of polymineral fine grain revealed a consistent natural inherited dose of approximately 5 Gy for pIRIR180 and an unbleachable residual of around 2 Gy for IR50, with substantial fading rates in the latter. This led to a notable age overestimation when compared with bulk organic matter radiocarbon ages. To develop an appropriate dating approach, we evaluated the differential bleaching rates of feldspar IR50 and pIRIR180 signals, and corrected for modern inherited doses. Radiocarbon ages measured on the bulk organic carbon oxidised at 400 °C (LT 14C) increased very consistently with depth, allowing calculation of accumulation rates. The presence of the atmospheric radiocarbon bomb signal at depth indicated down-mixing of surface material into the profile, which caused negative (younger) age offsets. The offset-corrected radiocarbon-based age-depth model could be compared to the luminescence results.We show that a combination of LT 14C with polymineral pIRIR180 dating allows the development of age models for these deposits. This multi-chronological approach reveals that loess accumulation in the region was initiated around 4 ka, probably consisting of two main phases of loess accumulation at 4–3 ka and <1 ka. The initial phase matches the proposed onset of aeolian sand activity in the wider region, but post-dates local ice retreat by c. 3 kyr. The more recent phase of accumulation also matches the timing of increased sand accumulation in the region and likely coincides with Neoglacial to Little Ice Age ice advances, or even enhanced dust activity in the last decades.

Trawling-Induced Sedimentary Dynamics in Submarine Canyons of the Gulf of Palermo (SW Mediterranean Sea)

Bottom trawling in submarine canyons can affect their sedimentary dynamics, but studies addressing this topic are still scarce. In the Gulf of Palermo (NW Sicily, SW Mediterranean Sea), bottom trawling occurs on the continental slope, but principally concentrates within Oreto Canyon. Hydrographic profiles and time series data of temperature, turbidity, and currents obtained by a CTD probe and by moored instruments, respectively, revealed increased turbidity values and the presence of bottom and intermediate nepheloid layers coinciding with periods of bottom trawling activity. The delay between the onset of trawling activities along the Oreto canyon axis and the increase in water turbidity at the mooring location indicate that trawling resuspended particles are progressively advected down-canyon by hydrodynamic processes. Topographic waves and near-inertial currents seem to contribute to the sediment transport of resuspended particles as bottom and intermediate nepheloid layers. Results presented in this paper highlight the complex relationship between hydrodynamic processes and sediment resuspension by trawling in submarine canyons.

Chang'an decoction alleviates endoplasmic reticulum stress by regulating mitofusin 2 to improve colitis.

To evaluate the protective effects of Chang'an decoction (, CAD) on colitis, and investigate the potential mechanisms underlying these effects from the perspectives of endoplasmic reticulum (ER) stress induced by mitofusin 2 (MFN2). The composition of CAD was identified by liquid chromatography-mass spectrometry technology. A mice model of dextran sulfate sodium (DSS) induced colitis was established and therapeutic effects of CAD were determined by detecting body weight, disease activity index, colon length and histopathological changes. Then, the expression levels of MFN2, ER stress markers and Nucleotide-binding domain and leucine-rich repeat protein3 (NLRP3) relevant proteins were detected by polymerase chain reaction (PCR), Western blot, immunohistochemistry and immunofluorescence staining. Subsequently, knockdown and overexpression cell model were constructed to further investigate the underlying mechanism of MFN2 mediating ER stress and energy metabolism by PCR, Western blot, electron microscopy and reactive oxygen species (ROS) staining. Finally, inflammatory indicator and tight junction proteins were measured by PCR and immunofluorescence staining to evaluate the protective effects of CAD. Results showed that the indispensable regulatory role of MFN2 in mediating ER stress and mitochondrial damage was involved in the protective effects of CAD on colitis in mice fed with DSS. Network pharmacology analysis also revealed CAD may play a protective effect on colitis by affecting mitochondrial function. In addition, our data also suggested a causative role for MFN2 in the development of inflammatory responses and energy metabolic alterations by constructing a knockdown and overexpression cell model whereby alter proper ER-mitochondria interaction in Caco-2 cells. Furthermore, relative expression analyses of ER stress markers and NLRP3 inflammasome showed the onset of ER stress and activation of NLRP3 inflammasome, which is consistent with the above findings. In contrast, intervention of CAD could improve the mucosal barrier integrity and colonic inflammatory response effectively through inhibiting ER stress response mediated by MFN2. CAD could alleviate ER stress by regulating MFN2 to exert therapeutic effects on DSS-induced colitis, which might provide an effective natural therapeutic approach for the treatment of ulcerative colitis.

Postural Control During Single-Leg Landing in Female Athletes After Anterior Cruciate Ligament Reconstruction.

Secondary anterior cruciate ligament (ACL) injury is a complication of ACL reconstruction (ACLR), which may result from altered neuromuscular control affecting anticipatory postural adjustments (APAs) and compensatory postural adjustments (CPAs) required for maintaining balance during movement. However, it remains unclear how APAs and CPAs differ in single-leg landings post-ACLR compared to healthy subjects. The purpose of this study was to clarify the differences in muscle activities of APAs and CPAs, lower limb kinematics, and kinetics between athletes with a history of ACLR and healthy athletes during single-leg landing. Cross-sectional study. Eighteen female athletes were recruited and divided into ACLR (n = 9) and control groups (n = 9). The experimental task involved a single-leg landing from a 30 cm box. Joint angles and moments were determined using a 3-dimensional motion analysis system, while muscle activity was assessed using surface electromyography. Analysis intervals were divided into two phases: the APA phase (-150 ms to 50 ms) and the CPA phase (50 ms to 250 ms), with initial contact (0 ms) as the reference point. Muscle activity onset time was defined as the time when the baseline exceeded by the sum of mean values and 2 standard deviations. No significant differences were observed in muscle activity or onset time between the ACLR and control groups. However, an increased hip external rotation moment was observed during the CPA phase in the ACLR group. These findings suggest that APAs and CPAs of athletes who returned to sports more than 1 year post-ACLR may be similar. The increased hip external rotation moment in the ACLR group during the CPA phase could represent a specific compensatory strategy to decrease the hip internal rotation angle post-ACLR. III.

“In my Own Land, I Am Treated like a Foreigner”: Mining-Induced Challenges and Psychological Distress Among Farmers in Ghanaian Mining Communities

This study explored mining-induced challenges, experiences of psychological distress, and coping strategies among farmers in mining communities in Ghana. Using a qualitative research approach, in-depth interviews were conducted with 30 crop farmers in two mining communities in the Ellembelle District in the Western Region of Ghana. Thematic analysis of the data suggests that psychological distress is a common experience among farmers in mining communities. Farmers’ experiences of psychological distress emanate from various mining-induced challenges, including deprivation of vital resources, constraints on farming activities, economic hardship, and adjustment difficulties associated with displacement and resettlement. Faced with limited formal support services, farmers in mining communities employ religious faith, avoidance, and adaptation as mechanisms for coping with the adverse impacts of mining. The findings of this study suggest the need to address issues related to psychological well-being before and after the onset of mining activities in communities.

Internal structure of the volcanic island of Surtsey and surroundings: Constraints from a dense aeromagnetic survey

Surtsey, a young basaltic island off the south coast of Iceland, was built by volcanic activity in 1963–1967 from a pre-eruption oceanic seafloor depth of 130 m. An aeromagnetic survey was carried out in October 2021 over a 60 km2 area covering Surtsey and its surroundings. It aimed to explore the internal structure and the possible existence of basaltic intrusions associated with the five vents active at different times over the 3.5 years of eruptive activity. The survey line spacing was 200 m and the flying altitude was generally 90 m a.s.l. The strongest anomalies (amplitude ∼700 nT) are caused by the 30–100 m thick subaerially erupted lava field on the southern part of Surtsey, formed in two episodes of effusive activity:1964–1965 and 1966–1967. 2D spectral analysis and Euler deconvolution indicate that the causative bodies of anomalies outside the island of Surtsey are located within the uppermost 300 m of the seafloor and their horizontal dimensions are similar to or smaller than their depth. 3D forward modeling of the island and its surroundings, constrained by observations during the formation of the island and drill cores extracted in 1979 and 2017, is consistent with an absence, at all vents, of pillow lava and therefore effusive activity in their opening phases. However, the data support the existence of a 10–20 m thick pillow lava field on the seafloor, 2.5–3 km2 in area, extending about ∼1 km to the south of Surtsey. The field is considered to have been fed by magma reaching the seafloor via channelized intrusive flow through the foreset breccia constituting the submarine part of an emerging lava delta during the early stage of effusive eruption in May–July 1964. The general scarcity of significant magnetic bodies within the edifices is consistent with magma fragmentation dominating the submarine eruptions from the onset of activity. A small magnetic anomaly is observed over the submarine edifice of Surtla, built during short-lived activity over ∼10 days in 1963–1964. This anomaly is consistent with observed subaqueous weak or moderate explosive activity that may have allowed a dyke to be preserved within the submarine tephra mound. More violent Surtseyan activity was observed at other vents, however, and may have destroyed any initial dykes that, if preserved, might have been resolved magnetically. Indications of magnetized volcanic rocks of unknown age predating the Surtsey eruption are found beneath the flank of the ephemeral island of Jólnir, the southernmost of the Surtsey vents.

Determination of Earthquake Depths Using Data of Cross-Sectional and Areal Deep Seismic Studies in Siberia

Abstract ––Information on the distribution of earthquake hypocenters for many seismically active zones of Siberia remains insufficient, which is associated with sparse networks of seismological observations. The paper presents the results of determining the earthquake depths in several seismogenic areas in the Altai-Sayan region, Cisbaikalia, Transbaikalia, and Yakutia using the travel time of longitudinal refracted waves from the Mohorovičič discontinuity (Pn waves) from earthquakes and the recently obtained information about the deep structure of these regions. The depth determination algorithm is tested using data from aftershocks of large earthquakes occurring in Tuva in 2011 and 2012 (ML = 6.7 and 6.8), recorded both by the regional seismological network and by a local group of seismic stations. Different methods are applied to reveal that some aftershock depths have a close match, including those for the main shocks – the Tuva-1 and Tuva-2 earthquakes. Another good match of earthquake depths is obtained using Рn waves with materials from regional and detailed studies of the Baikal branch of the Geophysical Survey of the Russian Academy of Sciences in the Muyakan activation site in the Baikal rift zone. The resulting data complement the information on the hypocenter of the main shock and confirm the change of the Muyakan activation cluster from large depths to shallow ones since the onset of activation in 2014. New information on the earthquake depths using Рn waves is obtained in Yakutia along the border of the largest Eurasian and Okhotsk tectonic plates. It is revealed that they decrease to 6–12 km as compared to higher depths of 20–30 km in adjacent areas. The resulting series of new information on the distribution of earthquake hypocenters using Рn waves is extremely important mostly because it indicates the possibility of identifying and redefining earthquake hypocenters using previous seismological observations in seismically active zones of Siberia.

Visualizing the Active Site Oxyanion Loop Transition Upon Ensitrelvir Binding and Transient Dimerization of SARS-CoV-2 Main Protease

N-terminal autoprocessing from its polyprotein precursor enables creating the mature-like stable dimer interface of SARS-CoV-2 main protease (MPro), concomitant with the active site oxyanion loop equilibrium transitioning to the active conformation (E*) and onset of catalytic activity. Through mutagenesis of critical interface residues and evaluating noncovalent inhibitor (ensitrelvir, ESV) facilitated dimerization through its binding to MPro, we demonstrate that residues extending from Ser1 through Glu14 are critical for dimerization. Combined mutations G11A, E290A and R298A (MPro™) restrict dimerization even upon binding of ESV to monomeric MPro™ with an inhibitor dissociation constant of 7.4 ± 1.6 µM. Contrasting the covalent inhibitor NMV or GC373 binding to monomeric MPro, ESV binding enabled capturing the transition of the oxyanion loop conformations in the absence of a reactive warhead and independent of dimerization. Characterization of complexes by room-temperature X-ray crystallography reveals ESV bound to the E* state of monomeric MPro as well as an intermediate approaching the inactive state (E). It appears that the E* to E equilibrium shift occurs initially from G138-F140 residues, leading to the unwinding of the loop and formation of the 310-helix. Finally, we describe a transient dimer structure of the MPro precursor held together through interactions of residues A5-G11 with distinct states of the active sites, E and E*, likely representing an intermediate in the autoprocessing pathway.

Progesterone profiles in postpartum dairy cows with inflammatory disorders

The objective of this prospective cohort study was to determine if progesterone (P4) profiles differed between dairy cows with or without inflammatory disorders early postpartum. A total of 708 cows from 2 commercial herds were enrolled 3 wk before parturition and examined for clinical health disorders (difficult calving, retained placenta, metritis, displaced abomasum, mastitis, or lameness) until 5 wk postpartum. Serum haptoglobin (Hp) was measured in blood at 2 and 6 DIM (range ±2 d); metritis was assessed at 4, 8, 11, and 15 DIM; and purulent vaginal discharge and endometritis (≥6% PMN in endometrial cytology sampled by cytobrush) were assessed at 35 ± 3 DIM. As Hp ≥0.8 g/L or endometritis were associated with ovarian dysfunction in previous studies, cows with serum Hp ≥0.8 g/L at either time point and endometritis, regardless of clinical disease, were classified as the cohort with inflammatory disorders (INFLAM; n = 139). Clinically healthy cows without difficult calving, with singleton birth, with Hp <0.8 g/L at both sampling times, without endometritis or purulent vaginal discharge, and BCS ≥3.00 (1 to 5 scale) were classified as healthy (n = 133). Cows with only one of the 2 conditions (high Hp or endometritis) were excluded. Cohorts had serum P4 measured twice weekly from 35 to 70 DIM (±3 d), and the first detected luteal phase (LP) during the sampling period was defined as the period from onset of luteal activity (P4 increase to ≥1 ng/mL) until decline of P4 to <1 ng/mL. The odds of prolonged LP (≥21 d long), average LP length, peak P4, and time to P4 decline (hazard rate) were analyzed using multivariable mixed logistic, linear, or Cox proportional hazard regression models including INFLAM status, parity, sampling day (when applicable), and herd as a random effect considering the covariates of season, milk yield at first DHIA test, and DIM at onset of cyclicity or LP length (when applicable). Cows with INFLAM had greater odds of prolonged LP (LSM ± SEM; 67% vs. 37% ± 7%), greater average LP length (17 vs. 15 ± 2 d), lesser P4 at d 4 (4.6 vs. 5.5 ± 0.3 ng/mL) and d 7 (6.0 vs. 7.7 ± 0.3 ng/mL) of the LP, and lesser peak P4 (6.9 vs. 8.2 ± 0.3 ng/mL) during the LP than healthy cows. Status of INFLAM was associated with time to P4 decline in multiparous but not primiparous cows; the LP of INFLAM multiparous cows was less likely to have luteolysis (P4 decline) by d 14 (adjusted hazard ratio [AHR] and 95% CI: 0.54; 0.31 to 0.94) or by d 21 (AHR: 0.32; 0.12 to 0.84) than in healthy multiparous cows. In conclusion, postpartum cows with markers of systemic inflammation at wk 1 and uterine inflammation at wk 5 had altered luteal function (prolonged LP and lower P4 concentrations) before first breeding, which is a possible pathway linking postpartum health disorders and reduced fertility.

Dev-ResNet: automated developmental event detection using deep learning.

Delineating developmental events is central to experimental research using early life stages, permitting widespread identification of changes in event timing between species and environments. Yet, identifying developmental events is incredibly challenging, limiting the scale, reproducibility and throughput of using early life stages in experimental biology. We introduce Dev-ResNet, a small and efficient 3D convolutional neural network capable of detecting developmental events characterised by both spatial and temporal features, such as the onset of cardiac function and radula activity. We demonstrate the efficacy of Dev-ResNet using 10 diverse functional events throughout the embryonic development of the great pond snail, Lymnaea stagnalis. Dev-ResNet was highly effective in detecting the onset of all events, including the identification of thermally induced decoupling of event timings. Dev-ResNet has broad applicability given the ubiquity of bioimaging in developmental biology, and the transferability of deep learning, and so we provide comprehensive scripts and documentation for applying Dev-ResNet to different biological systems.