Activity Of Water-soluble Extracts Research Articles

The biological activity of fermented milk produced by Lactobacillus casei ATCC 393 during cold storage

Lactobacillus casei ATCC 393 is an important probiotic strain widely known in dairy technology. However, its capability to produce bioactive peptides from milk proteins has not been studied. The viability of the Lb. casei ATCC 393 strain and some physicochemical properties in fermented milk throughout storage for 21 days at 4 °C was evaluated; biological activity, i.e., antioxidant, angiotensin converting enzyme inhibitory and anticancer activities of water soluble extract and its filtrate (< 2 kDa; F1) were determined. Lb. casei counts remained over 9 log cfu g−1 during the storage period in fermented milk. These bioactivities were increased significantly (P < 0.01) during storage. F1 of fermented milk after three weeks of storage showed the highest bioactivity impact. De novo sequencing assay for peptide identification was applied to the mass spectrum of F1. The promising capability of Lb. casei ATCC 393 to release bioactive peptides from milk proteins was demonstrated.

Assessment of oral hypoglycaemic activity of water soluble extract of aloe vera and its interaction with glipizide in alloxan induced diabetic Wistar Rats

Background: Assessment of oral hypoglycaemic activity of water soluble extract of Aloe vera and its interaction with Glipizide in Alloxan induced diabetic Wistar rats.Methods: Under standard condition of testing, alloxan induced diabetic rats were kept in fasting state of 8 hours and then glucose in dose of 1gm/kg was given PO by feeding tube. There after water soluble extract of aloe vera in dose of 200mg/kg BW dissolves in 1ml of Distilled water (DW) to test group 1, Glipizide in dose of 2.5mg/kg BW dissolved in 1ml DW was given to standard group. Test group 2 received combination of Aloe vera 200mg/kg BW and Glipizide in dose of 2.5mg/kg BW dissolved in 1ml Dw. Control group received 1ml of Distilled water. Blood sugars levels of all Rats in each group were checked with Glucometer.Results: Clinically and statistically significant glucose lowering effect was observed in groups administered with Aloe vera and better results were observed in group receiving Aloe vera and Glipizide combination.Conclusions: Water soluble extract of aloe vera has significant hypoglycaemic activity and the results showed that co-administration of Aloe vera and Glipizide has superior hypoglycaemic activity compared to Aloe vera and Glipizide given Individually.

In vitro investigation of anticancer and ACE-inhibiting activity, α-amylase and α-glucosidase inhibition, and antioxidant activity of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk

This study aimed to investigate in vitro the health-promoting benefits (anticancer activity, α-amylase and α-glucosidase inhibition, angiotensin-converting-enzyme (ACE)-inhibition, antioxidant and proteolytic activity) of camel milk fermented with indigenous probiotic strains of Lactobacillus spp., compared with fermented bovine milk. The three camel milk probiotic strains Lb. reuteri-KX881777, Lb. plantarum-KX881772, Lb. plantarum-KX881779 and a control strain Lb. plantarum DSM2468 were employed to ferment camel and bovine milks separately. The proteolytic and antioxidant activity of water soluble extracts (WSEs) from all fermented camel milks were higher than those of fermented bovine milk. α-Amylase inhibition of WSEs were >34% in both milk types fermented with all strains during storage periods, except the WSE of camel milk fermented by Lp.K772. The highest ACE-inhibition of the WSE from camel milk fermented by Lr.K777 was >80%. The proliferations of Caco-2, MCF-7 and HELA cells were more inhibited when treated with the WSE of fermented camel milk.

BIOLOGICAL ACTIVITY OF SECRETION OF PLANT MATTER AND SOIL FROM HYSSOP SPECIES (HYSSOPUS OFFICINALIS)

Hyssop (Hyssopus officinalis L.) comes from Mediterranean region. Cultural and wild forms grow in Europe, North America, and the Forest-Steppe and Steppe zones in the Eastern Europe. It is a perennial herbaceous semi-bush that ranges from 50 to 80 cm in height. In the study we performed experimental work on the basis of M.M. Gryshko National Botanical Garden of National Academy of Sciences of Ukraine (Kyiv city) and agrarian and biological station of Pavlo Tychyna Uman State Pedagogical University (Uman city, Cherkasy region). Allelopathic activity of water soluble (WSS), alcohol soluble (ASS) and volatile secretions (VS) was determined by biological tests (Hrodzynskyi, 1991) – one-day seedlings of garden cress (Lepidium sativum L.) in the model experiments. Comparative analysis of the activity of water soluble extracts of Hyssop showed that water soluble secretions from both above-ground organs and roots were characterized by high phytotoxicity recorded at the beginning of plant growth. Wheat coleoptiles were more tolerant to secreting; their activity was low in the condition of Kyiv city as well as Uman city. The authors have defined the following results regarding the reaction of winter wheat roots: secretions of all plant organs in the condition of Kyiv city were phytotoxic, but water soluble secretions from roots and stems had a stimulating effect and, on the contrary, secretions from leaves and inflorescences showed inhibitory impact in terms of Uman city. Most of plant ekzometabolites are accumulated in the soil of rhizosphere rhizoplan and contribute to its enrichment with organic compounds and mineral elements and also accumulation of humus. Received results showed dependence between accumulation of phytotoxic substances and soil type and climatic conditions. The conclusions are as follows. Different types of plant secretions of Hyssopus officinalis L. species during a vegetation period are analyzed. The largest number of substances with inhibitory action was localized in leaves and inflorescences. Blossoming phase was a demonstrative phase concerning allelopathic activity. Roots of garden cress were the most sensitive to secretions among selected test-objects. It was found that soil of rhizosphere and space between rows during a vegetation period accumulated colins, content of which depended on environmental factors and secretory functions of plants.

주목나무열매 유래 수용성 다당의 생리활성

주목나무열매 유래 수용성 다당의 생리활성

Effect of extracts from Radix Trichosanthis on the expression of HBsAg and HBeAg in HepG2.2.15 cells

To investigate the effect of extracts with water and alcohol from Radix Trichosanthis on the cell survival and the expression of hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg) in HepG2.2.15 cell supernatant. The cell survival rate of HepG2.2.15 cells was detected by MTT assay. The HBsAg and HBeAg in HepG 2.2.15 cell supernatant were evaluated by enzyme linked immunosorbent assay. The water and alcohol soluble extracts from Radix Trichosanthis significantly inhibited the levels of HBsAg and HBeAg in HepG2.2.15 cells in a time-and-concentration-dependent manner. However, the therapeutic index of extracts with water from Radix Trichosanthis was better than that in the alcohol group. The activity of water-soluble extract from Radix Trichosanthis is stronger on anti-hepatitis B virus than that of the alcohol-soluble extract.

A note on the in vitro macrophage-stimulating activity of water-soluble extracts from mycelium of Pleurotus spp.

Since macrophages have been suggested to play important roles in immunological surveillance, the purpose of this study was to assess the in vitro effects on macrophage activation of five water-soluble fractions (F-I to F-V) extracted from mycelium of the edible mushroom Pleurotus spp., strain P-184 (P. ostreatus× P. pulmonarius hybrid). After incubation of murine peritoneal macrophages (1×105 cells/well) with mushroom fractions (50 µg/culture) or lipopolysaccharide (LPS) (10 and 100 µg/culture) used as a positive control for 48 h, the glucose consumption of the macrophages significantly increased to 1.8–2.9 times that of the control without samples. The lysosomal enzyme (acid phosphatase) activity, determined in cell lysate by the amount of nitrophenol released per 60 min, was also enhanced positively to 133–184%, compared to saline control group. The results suggest that Pleurotus fractions possessed the ability to activate macrophages in vitro and these effects may have importance in the immunopotentiating activity.

Assessment of the immunomodulatory activity of cheese extracts by a complete and easy to handle in vitro screening methodology

A simple and rapid screening methodology based on the in vitro culture of murine hybridoma and human T-lymphocytes was developed to assess the potential immunomodulatory activity of water-soluble extracts (WSE) from cheese. The two immune cell lines were cultured in microplates with or without cheese WSE. The proliferation and the metabolic activity of cells were monitored at their different growth phases by the BrdU (5-bromo-2'-deoxyuridine) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide] assays, respectively. WSE from Abondance cheese enhanced DNA synthesis and the metabolic activity of the hybridoma cells (1.2-and 1.3-to 1.6-fold of the control, respectively) and also of the T lymphocytes (1.5-fold of the control) for almost all of the dilutions tested. To evaluate the validity of the results obtained following this screening methodology, hybridoma and T-lymphocyte cells were cultivated in 50 ml flasks: the maximal cell density was increased by about 10-16% in presence of cheese WSE for both cell lines and the antibody production by the hybridoma cells was increased by 50%.

納豆菌及びテンペ菌発酵おから由来水溶性物質の抗酸化性

納豆菌及びテンペ菌で発酵したおから(ON及びOT)から水溶性抗酸化物(WSM)を調製し,各試験における抗酸化力をを測定し,おから水溶性抗酸化物(OK-WSM)と比較検討した.1. リノール酸の過酸化に対する各WSMの抗酸化力を比較したところ,リノール酸/エタノール系ではON-WSMが,リノール酸-H2 O2/FeCl2系ではOT-WSMが最も強く,納豆菌及びテンペ菌発酵によるWSMの抗酸化力増大が確認された.2. OT-WSMのラジカル捕捉能は他のWSMに比べ高く,Fe2+に対する結合性もOK-WSMの5.6倍に増加していた.Fe2+存在下での抗酸化試験ではOT-WSMはトコフェロールとほぼ同等の抗酸化性を示した.3. 各WSMの過酸化水素消去活性を測定した結果,テンペ菌発酵ではその活性が減少したが,納豆菌発酵では活性の増大が確認できた.4. WSM中の大豆由来抗酸化性物質含量は納豆菌及びテンペ菌発酵により減少していた.納豆菌発酵ではWSMの収量,粗タンパク質含量及びアミノ酸含量が増加し,テンペ菌発酵ではアミノ酸含量の増加が認められた.おから発酵物からのWSMの抗酸化成分については引き続き検討の予定である.