Activity Of Toothpastes Research Articles

Antimicrobial Effect of Toothpastes on Dental Caries

Dental caries is an infectious disease of teeth which end up in the destruction of dental hard tissue. Poor oral hygiene is one of the major factor for accumulation of microorganisms and their harmful effects. The current study was done on antimicrobial efficacy of different brands of toothpastes against organisms isolated from patients having dental caries and comparative analysis has been made between chemical and herbal based toothpastes. Primary screening of organisms had been carried out on sterile Nutrient Agar medium and five isolates (A1, A2, A3, A4 and A5) were obtained. Antimicrobial activity of toothpastes was checked using Agar well diffusion assay. In chemical based toothpastes, Close-up showed the maximum zone of inhibition of 30mm against A1 isolate and minimum efficacy was observed with Pepsodent with zone of 7mm against A4 isolate. In herbal based toothpastes, Babool showed highest effectiveness with zone of 28mm against A3 isolate and minimum with zone of 10 mm with Dabur red and Himalaya against A1 and A3 isolate. The comparative analysis showed that chemical based toothpastes are more effective than herbal based toothpastes. Further studies on probiotic approach should be done which has been a popular method used to affect oral microbial community.

A new <i>in vitro</i> method for evaluating the antimicrobial activity of toothpaste

A new <i>in vitro</i> method for evaluating the antimicrobial activity of toothpaste

Assessment and comparison of toothpastes for pH and antibacterial effect against Streptococcus mutans: An in vitro study

Introduction: One of the universally practiced oral hygiene measures is tooth brushing with toothpaste. Each toothpaste is composed of different ingredients. The extent of the antimicrobial activity of toothpastes against cariogenic bacteria is still unclear. Toothpaste with the correct pH will help to neutralize the acidic activity of the oral cavity. However, there are lack of studies to determine pH of different toothpastes. Aim: The aim is to assess and compare the commercially available toothpastes for pH and antibacterial activity against Streptococcus mutans. Materials and Methods: Commercially available toothpastes composed of varying ingredients available in the local market were included in the study. The pH of the toothpastes was assessed using pH meter. The antimicrobial activity was assessed by making serial dilutions for each selected toothpastes in 1:1, 1:2, 1:4, and 1:8 ratio using distilled water. At different concentrations, the antimicrobial activity of the toothpastes against S. mutans was determined by the agar well diffusion method. The diameter of zones of inhibition (mm) was measured for assessing the antimicrobial activity. Results: The mean pH of toothpastes ranged from 7.262 ± 0.414 to 7.729 ± 0.534. All toothpastes showed antimicrobial activity against S. mutans in all four dilutions 1:1, 1:2, 1:4, 1:8. Data were analyzed using the Statistical Package for the Social Sciences version 22 software (IBM Corporation). - Analysis of Variance was used to analyze data. Conclusion: The present study showed that the pH of all the toothpastes are above that of critical pH. All the toothpastes had shown antimicrobial activity against S. mutans at varying dilutions.

Comparative analysis of the antibacterial effect of preventive toothpastes with use of automated microorganism growth control system

The article presents experimental data of comparative assessment of antibacterial activity of prophylactic toothpastes. As a models for growth curves construction with help of innovative technology of microorganisms automated cultivation in bioreactor used strains Staphylococcus aureus, Streptococcus salivarius; Streptococcus mutans. The results of growth curves study of microorganisms under impact of active components of preventive toothpastes have shown the presence of bacteriostatic activity of these toothpastes. It is shown that investigated toothpastes differ from the other by more inhibitory activity on growth of main cariogenic type Streptococcus mutans compared with representatives of normal microbiota.