Surfactant Activity Research Articles

Synthesis, characterization, interaction with anionic dye, biodegradability, and antimicrobial activity of cationic surfactants: quaternary hydrazinium derivatives

Synthesis, characterization, interaction with anionic dye, biodegradability, and antimicrobial activity of cationic surfactants: quaternary hydrazinium derivatives

Catalytic Performance of a Recombinant Organophosphate-Hydrolyzing Phosphotriesterase from Brevundimonas diminuta in the Presence of Surfactants

Phosphotriestease (PTE), also known as parathion hydrolase, has the ability to hydrolyze the triester linkage of organophosphate (OP) pesticides and chemical warfare nerve agents, making it highly suitable for environment remediation. Here, we studied the effects of various surfactants and commercial detergents on the esterase activity of a recombinant PTE (His6-tagged BdPTE) from Brevundimonas diminuta. Enzymatic assays indicated that His6-tagged BdPTE was severely inactivated by SDS even at lower concentrations and, conversely, the other three surfactants (Triton X-100, Tween 20, and Tween 80) had a stimulatory effect on the activity, especially at a pre-incubating temperature of 40 °C. The enzyme exhibited a good compatibility with several commercial detergents, such as Dr. Formula® and Sugar Bubble®. The evolution results of pyrene fluorescence spectroscopy showed that the enzyme molecules participated in the formation of SDS micelles but did not alter the property of SDS micelles above the critical micelle concentration (CMC). Structural analyses revealed a significant change in the enzyme’s secondary structure in the presence of SDS. Through the use of the intentionally fenthion-contaminated Chinese cabbage leaves as the model experiment, enzyme–Joy® washer solution could remove the pesticide from the contaminated sample more efficiently than detergent alone. Overall, our data promote a better understanding of the links between the esterase activity of His6-tagged BdPTE and surfactants, and they offer valuable information about its potential applications in liquid detergent formulations.

Modulation of aggregation behavior, antimicrobial properties and catalytic activity of piperidinium surfactants by modifying their head group with a polar fragment

To establish the effect of surfactants structure on their functional activity a number of alkyl-N-hydroxyethylpiperidinium bromides with different length of the hydrophobic tail were synthesized and characterized. Aggregation parameters of these surfactants were determined using tensiometry and fluorimetry. It was shown that the piperidinium surfactants, including those functionalized with OH groups, show a high antimicrobial effect at concentrations lower than critical micelle concentration for both bacteria and fungi, with the low hemolytic activity revealed. In addition, these surfactants were studied as micellar catalysts in alkaline hydrolysis of carboxylic acid esters. Micellar catalytic effect increases with an growth in the length of the alkyl tail, as well as with an increase in the number of hydroxy groups in surfactant molecule and can exceed two orders of magnitude.

Antimicrobial and bacteriostatic activity of surfactants against B. subtilis for microbial cleaner formulation.

Cleaning products containing live bacteria that form spores of Bacillus spp. as active substances are becoming increasingly common in probiotic cleaner formulation. The quality of cleaning performance for the production of probiotic cleaners does not only depend on the potential of the bacterial strains used, but also on the chemical components of the formulations. In this study, the surfactants and other additives were investigated as biocidal or bacteriostatic against B. subtilis, and the viability of B. subtilis was examined at different pH ranges for microbial cleaner formulation. As a result, it was discovered that the B. subtilis, which can be used in the microbial cleaner formulation, shows higher growth and viability at the neutral pH, and it passes into the death phase at pH 3. According to antagonistic activity results, the Gram-positive S. aureus and K. pneumoniae were the most sensitive bacteria while B. cereus was the most resistant bacteria. The anionic surfactants such as linear alkylbenzene sulfonic acid and sodium lauryl ether sulfate act as bacteriostatic on Bacillus spp. and do not cause cell death. In the view of these results, the usage of appropriate bacterial cultures and the correct stabilization of the formulations are also critical elements in the development of microbial cleaner formulations.

Effects of surfactant type and structure on properties of amines for carbon dioxide capture

Nonionic and cationic surfactants were tested for potential use as additives in monoethanolamine (MEA) solution to improve the wetting of solid packing materials (steel, stainless steel, or aluminum) by the solution. This is expected to improve the mass transfer of carbon dioxide (CO2) to the amine solution. The surface activities of surfactants were evaluated based on their ability to lower MEA’s surface tension, which is related to spreading and wetting of MEA on packing materials, to maximize the interfacial area for absorbing CO2. Effects of the surfactants on kinematic viscosity, foam ability and foam stability, CO2 equilibrium loading, and the initial CO2 absorption rate – deemed as the key properties of the MEA solution – were also measured. The results revealed that the surfactant could reduce surface tension of the MEA by 14–56%. Such reduction helped lower the contact angle of the MEA on the test materials by 40–55%. The surfactants did not significantly affect kinematic viscosity of MEA solution, but only slightly increased the amine’s viscosity. Neither were CO2 equilibrium loading nor initial CO2 absorption rate by MEA affected by any of the surfactants. A couple of surfactants, specifically a nonionic surfactant with a small hydrophilic head, and a cationic surfactant with a short linear chain, even slightly increased the initial CO2 absorption rate by MEA. However, the concentration of a surfactant in the MEA solution should be kept below 1 CMC to benefit from improved wetting while keeping foaming to a minimum.

Biosurfactant as an Environmental Remediation Agent: Toxicity, Formulation, and Application in the Removal of Petroderivate in Sand and Rock Walls

The exploitation and transportation of petroleum products are important reasons for environmental concern since they are the major causes of accidents involving oil spills. Therefore, the biosurfactant produced by the bacterium Bacillus cereus UCP1615 was applied in the removal of motor oil from sand and stones from coastal regions. Bioassays carried out with the microcrustacean Artemia salina and three vegetables' seeds showed that the biosurfactant has reduced toxicity. The application of the biosurfactant in crude and isolated form, as a removal agent, was carried out in static and kinetic tests using sand contaminated with motor oil, in packed columns, and in flasks under orbital agitation, respectively. The results showed that the biosurfactant removed 63.0 ±2.1% of the hydrocarbon in the static test and 84.0 ±2.2% in the dynamic test. In tests using rock wall samples, the removal rates reached 69.0 ±2.0%. Conservation tests carried out during 120 days demonstrated that the surfactant activities were maintained, especially in the presence of the preservative potassium sorbate. The B. cereus biosurfactant has proven to be a biocompatible, effective, and stable alternative for applications in environments contaminated with hydrophobic compounds and can be industrially stored for application in the event of possible environmental disasters.

Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

Microbial surfactants (biosurfactants) are multifunctional preparations due to a combination of physicochemical (reduction of surface and interfacial tension, emulsifying activity) and biological (antimicrobial and antiadhesive activity, the ability to destroy biofilms) properties. However, the disadvantage of biosurfactants synthesized as a complex of compounds is the possibility of changing the biological activity depending on the conditions of producer cultivation. Aim. To study the effect of potassium and sodium cations on the NADP+-dependent glutamate dehydrogenase activity of cell-free extract of Acinetobacter calcoaceticus ІМV B-7241 with subsequent appropriate modification of the nutrient medium composition and determination of antimicrobial and anti-adhesive activity of surfactant synthesized. Methods. A. calcoaceticus ІМV B-7241 strain was grown in media containing 2% of sunflower oil waste as a carbon source, as well as various concentrations of potassium and sodium chloride (basal – 1.0 g/l NaCl, medium #1 that did not contains NaCl, medium #2 in which the concentration of NaCl was 2.0 g/l, medium #3 in which the concentration of NaCl and KCl was 1.0 g/l each). The surfactants were extracted from the supernatant liquid culture with a modified Folch mixture. Antiadhesive activity and the degree of biofilms degradation were determined by spectrophotometric method, antimicrobial activity − by the indicator of the minimum inhibitory concentration (MIC). Activity of enzymes of surface-active aminolipids biosynthesis (NADP+-dependent glutamate dehydrogenase) and glycolipids (phosphoenolpyruvate (PEP) carboxylase, PEP-synthetase, PEP-carboxykinase, trehalose phosphate synthase) were analyzed in cell-free extracts obtained after the destruction of cells by ultrasound. Results. It was found that potassium and sodium cations in concentrations of 50 and 100 mM are inhibitors of NADP+-dependent glutamate dehydrogenase, and in lower concentrations (5–20 mM) – activators of this enzyme, as well as PEP-carboxykinase and PEP-synthetase. The increase in the biosurfactant concentration to 6.1−7.7 g/l during cultivation of A. calcoaceticus ІМV B-7241 in medium #1 and #3 was due to the predominant synthesis of glycolipids under such conditions, which was evidenced by the increase in 1.8−6.5 times in the activity of PEP-carboxylase, PEP-carboxykinase, PEP-synthetase and trehalose phosphate synthetase compared to the indicators on the basal medium. The concentration of surfactants synthesized in the basal medium was 3.6 g/l, but such surfactants were characterized by the highest antimicrobial and anti-adhesive activity. Their MIC against the test-cultures of studied bacteria (Pseudomonas sp. MI-2, Bacillus subtilis BT-2, Escherichia coli IEM-1, Staphylococcus aureus BMS-1, Enterobacter cloaceae C-8) and fungi (Candida albicans D-6, Rhizopus nigricans P1, Aspergillus niger P-3, Fusarium culmorum T-7) were 0.88−56 μg/ml and were by 2−3 orders of magnitude lower compared to established for surfactants synthesized in modified media #1–3. In the case of treatment of abiotic materials with surfactant solutions obtained on the basal medium, the adhesion of bacteria and fungi was on average 10–20% lower than after surface treatment by the surfactant synthesized in modified media. In the presence of 148−296 μg/ml of surfactants obtained in the basal medium, destruction of S. aureus BMS-1 and B. subtilis BT-2 biofilms was 45−66%, and C. albicans D-6 yeast – 39−44%. Under the action of similar concentrations of surfactants synthesized in modified media, the destruction of bacterial and yeast biofilms was lower: 6-52 and 20–46%, respectively. Conclusions. The obtained results are consistent with the data of our previous studies on the possibility of regulating the antimicrobial and antiadhesive activity of surfactants in the process of producer cultivation by changing the content of cations in the medium, which are inhibitors/activators of enzymes responsible for the synthesis of components of the surfactants complex, which have certain biological properties.

Influence of surface tension on the characteristics of FTIR spectra on the example of saliva

The aim of this work was to test the hypothesis about the effect of surface tension of biological fluids on the characteristics of IR spectra using saliva as an example. The study involved 100 volunteers without clinically significant diseases. Saliva samples were collected from all participants, a parallel determination of surface tension, biochemical composition was carried out, and IR spectra were obtained. Comparison of the results was carried out using cluster analysis, principal component analysis and correlation analysis. The existence of a relationship between the characteristics of absorption bands in IR spectra and surface tension has been established using saliva as an example. It is shown that, first, a change in the surface tension affects the shift of the absorption bands in the spectra, while the intensity and area of ​​the corresponding absorption bands change to a lesser extent. Combinations of the characteristics of the IR spectra have been revealed that provide the maximum correlation relationship with both the total volumetric content of surfactants and their adsorption activity. Correlations with total surfactant content include absorption band characteristics corresponding to proteins, lipids and phospholipids. Whereas the adsorption activity of surfactants correlates mainly with the absorption bands of proteins in saliva. The revealed patterns are substantiated taking into account the biochemical composition of saliva, depending on the surface tension.

Hyperbranched polymer surfactant: synthesis, characterization and surface tension activity

A series of hyperbranched polymer surfactants (HBP-C8, HBP-C12 and HBP-C16) were synthesized by the reaction between hydroxyl-terminated hyperbranched polymers (HBP) and fatty acyl chloride. The structure of obtained hyperbranched polymer surfactant was characterized by FTIR, NMR and GPC. The results showed that the products have amphiphilic structure. The thermal property of the hyperbranched polymer surfactant investigated by DSC and TGA was strongly influenced by the length of end alkyl chain. Surface activity of hyperbranched polymer surfactant was analyzed by surface tension method and UV spectrophotometry, respectively. The results showed that hyperbranched polymer surfactant took on better surface activity, which can effectively reduce the surface tension of the water. The hyperbranched polymer surfactant has a lower critical micelle concentration (CMC) and displays single molecular micellar properties, which can package small hydrophilic molecules in relatively low concentration.Graphical abstract

Preparation of Polypeptide Surfactants Using Chromium‐Containing Waste Leather: Effect of Hydrophilic and Lipophilic Groups

AbstractPolypeptide surfactants with different lengths of hydrophilic groups and lipophilic groups were synthesized from collagen hydrolysate polypeptides by the Schotten‐Baumann reaction. Collagen hydrolysate polypeptides were prepared through alkaline hydrolysis of chromium‐containing leather wastes, followed by changing the molecular weight of the collagen hydrolysate and the carbon chain length of the acid chloride. Fourier transform infrared spectroscopy, confirmed the successful synthesis of the polypeptide surfactants. Furthermore, surface activities of surfactants were tested to reflect the influence of the length of hydrophilic and lipophilic groups on properties, such as surface tension, foamability, foam stability, wettability, and Hydrophile–Lipophile Balance value. The results indicated that for surfactants prepared from collagen hydrolysate, a shorter hydrophilic group length resulted in lower surface tension and better foamability, foam stability, and a wetting ability which indicates the surfactants can be used as wetting agents. In contrast, the surfactant with a greater lipophilic group length had lower surface tension and poorer foamability, foam stability, and wetting ability. The presence of the C=C bond of the oleoyl lipophilic group decreased surface tension. The polypeptide surfactants prepared from collagen hydrolysate not only can effectively utilize chromium‐containing leather solid waste produced, but also avoid environmental pollution and promote the sustainable development of the leather industry.

Budget impact analysis of surfactant therapy for bronchiolitis in critically ill infants: the Colombian National Health System perspective

BackgroundSevere bronchiolitis requiring mechanical ventilation was associated with an absence of surfactant activity and phosphatidylglycerol, causing airway obstruction in acute bronchiolitis. Exogen surfactant in mechanically ventilated infants decreased duration of stay in the intensive care unit and had favorable effects on oxygenation and carbon dioxide removal. This study aimed to evaluate the budget impact of surfactant therapy for bronchiolitis in critically ill infants in Colombia.MethodsBudget impact analysis was performed to estimate the economic impact of surfactant therapy (ST) for the treatment of infants with a diagnosis of bronchiolitis, requiring mechanical ventilation. The analysis considered a 4-year time horizon and Colombian National Health System perspective. The model estimated drug costs associated with current scenario using humidified oxygen or adrenaline nebulization, and new scenario adding exogen surfactant. The size of the target population was calculated using epidemiological national data. Univariate one-way sensitivity analyses and scenario analyses were performed.ResultsIn the base-case analysis the 4-year costs associated to ST and no-ST were estimated to be US$ 55,188,132 and US$ 55,972,082 respectively, indicating savings for Colombian National Health equal to US$ 783,950 if ST is adopted for the routine management of patients with bronchiolitis requiring mechanical ventilation. In the one-way sensitivity analysis, only increases in the cost of the surfactant drug and cost or length of stay in the pediatric intensive unit reduce the potential savings of ST.ConclusionST was cost-saving in emergency settings for treating infants with severe bronchiolitis requiring mechanical ventilation. This shift in treatment approach proved to be economically favorable in the Colombian context.

Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.

Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies. Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients. Pulmonary surfactant is crucial to optimal lung function and has not been investigated in MPS IIIA. We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age). Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue. There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF. Phospholipid composition remained unchanged, but BALF total phospholipids were reduced (49.70%) in MPS IIIA. There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice. Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling. Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.

Surface Activity and Structure of Temperature-Responsive Polymer Surfactants Based on PNIPAm at the Air/Solution Interface.

Thermally sensitive polymers have attracted tremendous interest in the design of stimulus-responsive surfactants. In this article, poly(propylene oxide)-b-poly(N-isopropylacrylamide) (PPO-b-PNIPAm) with different block lengths of PNIPAm was synthesized through atom transfer radical polymerization (ATRP). Different from commercial Pluronic surfactants, four distinct sections appeared in the decrease of surface tension with concentration. First, with increasing concentration, the amount of adsorbed polymers increased and the surface tension decreased sharply until a plateau was reached, which was caused by the rearrangement of methyl groups. The increasing adsorbed amount of PPO-b-PNIPAm resulted in the rearrangement of isopropyl groups, which changed from a lying down or horizontal conformation to a standing up or vertical conformation. This behavior led to the decrease in surface tension in part III until the critical micelle concentration (CMC) was reached. The surface tension of PPO-b-PNIPAm was thermally responsive. Except for the hysteresis observed in the first cycle, the surface tension was reversible during the heating-and-cooling cycles. At low concentrations, the low surface tension at higher temperatures was mainly caused by the increasing adsorption amount and ordered arrangement of methyl groups, while the standing up conformation of isopropyl groups at higher concentrations resulted in the low surface tension observed at high temperatures.

QSAR modeling for predicting the antifungal activities of gemini imidazolium surfactants against Candida albicans using GA-MLR methods

QSAR modeling for predicting the antifungal activities of gemini imidazolium surfactants against Candida albicans using GA-MLR methods

Binding Interactions Between Tetracaine Hydrochloride and Biocompatible Imidazolium-Based Gemini Surfactants in Aqueous Solutions

The low solubility of drugs in an aqueous medium causes their precipitation before reaching the specific target sites leading to various undesirable side effects. Thus, there is a need for designing and formulating delivery agents which not only carry a drug to its site of action but also improve its solubility. The study of interactions between a drug and its carrier becomes significant. Pertinently, the present study encompasses the interactions between aqueous solutions of a local anesthetic drug, tetracaine hydrochloride (TC) and a non-cytotoxic imidazolium gemini surfactant, [CnIm-3OH-ImCn]Br2 (n = 12, 14, 16). The results were established on the basis of various parameters calculated from measurements using conductivity, fluorescence, UV–visible spectroscopy and cyclic voltammetry (CV). The parameters obtained from conductivity measurements showed attractive interactions (synergism) between TC and [CnIm-3OH-ImCn]Br2 whereas the UV–visible and CV measurements indicated a 1:1 stoichiometry in their complex formation. The binding of drug with gemini surfactants was found to decrease as the surfactant chain length increases. The hydrophobic, cation-π and H-bonding modes of interactions between drug and gemini surfactant aid in their binding process. The antibacterial activity of gemini surfactants was tested against both strains (gram-positive and gram-negative) of bacteria and found to be effective. Thus, these interaction studies offer new opportunities to bring about advancements in the biomedical and pharmaceutical fields.

Antifungal activity of amino-alcohols based cationic surfactants and in silico, homology modeling, docking and molecular dynamics studies against lanosterol 14-α-demethylase enzyme

Fungi are being responsible for causing serious infections in humans and animals. The opportunistic microorganisms provoke environmental contaminations in health and storage facilities to represent a serious concern to health security. The present work investigates the antifungal activity of two amino-alcohols based cationic surfactants such as C n EtOH, C n PrOH (with n = 14 and 16 are the carbon numbers of alkyl chain and EtOH = Ethanol and PrOH = Propanol) against a collection of different Candida species (Candida tropicalis, Candida albicans, Candida auris, Cyberlindnera jadinii, Candida parapsilosis, Candida glabrata and Candida rugosa) respectively. The amino-alcohols based cationic surfactants exhibited good antifungal activity against all Candida strains tested with minimum inhibitory concentrations (MIC) ranging from 0.002 to 0.30 mM. The MIC evaluation shows an increase as a function of the hydrophobicity of all inhibitors against the majority of the Candida strains tested. The different location of the alcoholic OH function in the polar head shows the influence on the availability of N+ responsible for electrostatic interactions with the candidate’s cell walls, which remains a very important step in the mode of action of quaternary ammonium cationic surfactants. Hence, a 3D structure of lanosterol 14-α-demethylase enzyme from C. auris was constructed by homology modeling using an online SWISS-MODEL server. The predicted model was analyzed by serval servers. Furthermore, a molecular docking study was carried out to better understand the binding mechanism of lanosterol homologous protein with surfactant ligands. Then, the docked complexes lanosterol–surfactants were refined by the molecular dynamic simulation to analyze their interaction behavior during the simulation. Communicated by Ramaswamy H. Sarma

BASF invests in biosurfactants

BASF is deepening its involvement in glycolipid surfactants with two deals. BASF has taken a stake in Allied Carbon Solutions, becoming the Japanese sophorolipid specialist’s largest shareholder. In 2020, BASF and Allied launched a sophorolipid ingredient in Asia called BioToLife, which the firms say offers surfactant and antimicrobial action. Separately, BASF and the English biosurfactant maker Holiferm will jointly develop other glycolipid surfactants for use in home care, personal care, and industrial formulations. Both BASF partners make their surfactants by fermentation.

Mixing of Surfactin, an Anionic Biosurfactant, with Alkylbenzene Sulfonate, a Chemically Synthesized Anionic Surfactant, at the n‐Decane/Water Interface

AbstractBiosurfactants show synergic effects with synthesized surfactant in reducing hydrophobic/hydrophilic interfacial tension, while the understanding of the synergistic mechanism is limited. In the present work, mixed monolayers of surfactin and branched alkylbenzene sulfonate at the n‐decane/water interface were studied using atomistic molecular dynamics simulations, and the presence of surfactin affecting the microstructure and dynamic properties of the mixed monolayer was evaluated at molecular level. The density distributions of the surfactants along the direction normal to the interface, radial distribution functions of the surfactant head groups, hydrophobic contacts between surfactants, translational activities of both surfactants and counterions, and the dynamics of the hydrogen bonds formed between surfactant and water were calculated. The results suggested that the structure of the mixed monolayers was more compact than that of the individual system of alkylbenzene sulfonate and the interfacial tension was more efficiently reduced, and the translational activities of both surfactants within the mixed monolayers were much lower. The results implied that biosurfactant surfactin and alkylbenzene sulfonate mixed well at the n‐decane/water interface, though they were both anionic surfactants.

Effect of surfactant addition to Guar Gum and protection of mild steel in hydrochloric acid at high temperatures: Experimental and theoretical studies

Guar Gum (GG) is an established green inhibitor for mild steel (MS) corrosion in acidic media. The inhibitor action of GG is enhanced by the synergistic interaction with two non-ionic surfactants (Tween -80 and Triton-X). The synergistic pairs were investigated for MS corrosion in 0.5 M HCl using weight loss and electrochemical methods. The synergistic interactions were monitored via theoretical calculations using NBO analysis implemented in Gaussian-09 packages. AFM, SEM, and XPS spectra were carried out to understand the surface events. Computational calculations (DFT and Monte Carlo simulation) were performed to ensure the synergistic action of GG and surfactants. Experimental results revealed that GG:Tween -80 offers 97.13% and GG:Triton-X has 95.50% inhibition efficiency when used in 800: 5 ppm ration at 303 K. The synergistic pairs exhibited remarkable efficiencies at elevated temperatures also.

Hybrid Washer Fluid for Primary Cementing

This article presents the results on the basis of which a new hybrid drilling washer fluid was designed. The use of fluid from such a drilling washer increases the mud-cake removal efficiency. Its operation is based on both chemical and mechanical removal of the mud cake. This article presents a group of agents and admixtures of various solid fractions, the appropriate selection of which enabled the design of a hybrid drilling washer fluid. The liquid has much better washing parameters than the drilling washers used so far. The tests were carried out in a drilling fluid flow simulator. A significant improvement in the scrubbing mud-cake removal efficiency resulted from the action of surfactants and fine-grained abrasive additives. Their proper concentration was also very important. The hybrid drilling washer fluid was designed on the basis of tests measuring the adhesion of the hardened cement slurry to the rock from which the previously produced mud was removed. In this way, the effectiveness of the washing liquids was determined. Upon analyzing the obtained results and correlating them with the reference samples, one can see a significant improvement in the efficiency of the removal of the drilling sediment by the hybrid drilling washer fluid. The hybrid drilling washer fluid is an innovative solution because it combines chemical and mechanical action in the removal of drilling fluid. Additionally, such a washing liquid has not been used so far.