Soil Catalase Activity Research Articles

Organic fertilizers activate soil enzyme activities and promote the recovery of soil beneficial microorganisms after dazomet fumigation

Soil fumigation can reduce the impact of soil-borne diseases, weeds and insect pests on commercial crop production. Unfortunately, fumigation also kills beneficial microorganisms. In this study, we explored if dazomet fumigation could be used in combination with organic fertilizers (silicon fertilizer, potassium humate organic fertilizer, Bacillus microbial fertilizer, and mixtures of the last two) to reduce its impact on soil beneficial microorganisms. We evaluated the effects of adding these fertilizers after fumigation on the soil's physical and chemical properties and its enzyme activities, as well as its effects on the soil microbial communities under continuous production for >20 years. We found that fertilizers applied after fumigation increased the soil nitrate nitrogen content by 11.6%–29.4%, increased available potassium content by 5.6%–26.3% and increased organic matter content by 28.5%–48.8%. In addition, soil conductivity and water content increased significantly by 8.2%–26.5% and 8.0%–16.0%, respectively. The activities of soil catalase and soil sucrase were significantly increased by 6.2%–15.9% and 133.1%–238.5%, respectively. High-throughput DNA sequencing showed that fertilizers applied after fumigation increased the relative abundance of the phyla Proteobacteria, Actinobacteria and Ascomycota; and the genera Sphingomonas, Chaetomium and Mortierella. Silicon fertilizer applied after fumigation has the most significant promotion effect on soil micro-ecological health. The results showed that organic fertilizers applied after fumigation can improve the soil's fertility, activate soil enzyme activities and promote the recovery of soil beneficial microorganisms, which are all factors that improve crop quality and yield.

Effects of Bacillus fertilizer and agent on growth of young peach tree and soil environment under replant condition.

We analyzed the effects of Bacillus fertilizer and agent supplementation at different concentrations in the replanted soil by examining the growth of young peach trees, soil nutrients, enzyme activities, and fungal commu-nity. The aim of this study was to provide theoretical basis for alleviating peach replant diseases. One-year-old potted 'Yuanmeng' peach trees were subjected to eight treatments: replanted soil without supplementation (RS), non-replanted soil without supplementation (NS), replanted soil with 1%, 4%, 8% Bacillus fertilizer supplementation (BF1, BF2, BF3), and 0.1‰, 0.5‰, 1‰ Bacillus agent supplementation (B1, B2, B3), respectively. The results showed that shoot growth and root biomass under NS treatment were greater than that under other treatments, and the contents of soil organic matter, available nutrients and soil catalase activity were greatly enhanced. Peach trees treated with BF and B treatments showed better root development than RS, among which, BF3 and B1 treatments were the best, and B1 treatment had similar effects as NS treatment. Compared with RS, BF and B treatments significantly increased the activities of soil catalase and urease during new shoot development, while B treatment significantly increased soil sucrase activity after the growth cessation of new shoots. Bacillus fertilizer and agent increased soil fungal diversity and the relative abundance of Ascomycota, Chaetomium and Penicillium, and decreased that of Basidiomycota. Considering the comprehensive parameters, BF3 and B1 showed more significant effects. In summary, our findings indicated that the supplementation of Bacillus fertilizer and agent to replanted soil could improve soil fertility, fungal community, and root development of roots, and thus provided a feasible method to alleviate peach replant diseases.

Effects of wetland plant biochars on heavy metal immobilization and enzyme activity in soils from the Yellow River estuary.

The purpose of this study was to assess the effects of wetland plant biochars on the enzyme activity in heavy metal contaminated soil. The biochars were produced from Phragmites australis (PB), Suaeda salsa (SB), and Tamarix chinensis (TB) under different pyrolysis temperatures and times. The detected pyrolysis products showed that the ash, pH, electrical conductivity, and carbon content of the biochars increased significantly, while the production rate of the biochars decreased with increasing pyrolysis temperature and time. The results of the adsorption experiments indicated that biochar addition could effectively reduce the concentration of Pb and/or Cd in the Pb2+/Cd2+ single or mixed solutions, but the Pb2+ and Cd2+ in the mixed solution indicated a competitive adsorption. A 30-day incubation experiment was conducted using salt marsh soil amended with different biochar application rates to investigate the short-term effects of biochar addition on Pb and Cd immobilization. The PB and SB significantly immobilized Pb within the first 15days, but Pb remobilized within the next 15-day period. In contrast, TB addition did not significantly fix Pb. Moreover, biochar addition promoted the conversion of Cd from the residue to the less immobile fractions. The addition of three types of plant biochar had no significant effect on the urease activity in wetland soil but significantly increased soil sucrase activity. PB and SB significantly promoted catalase activity, while TB significantly inhibited soil catalase activity. According to the adsorption effect, the fixation effect, and the promotion of enzyme activities, the Suaeda salsa biochars are suitable for the remediation of heavy metal pollution in wetland soils.

Application of cotton straw biochar and compound Bacillus biofertilizer decrease the bioavailability of soil cd through impacting soil bacteria

BackgroundCd seriously threatens soil environment, remedying Cd in farmland and clearing the response of soil environment to modifiers in Cd-contaminated soils is necessary. In this study, the effects of cotton straw biochar and compound Bacillus biofertilizer used as modifiers on the biochemical properties, enzyme activity, and microbial diversity in Cd-contaminated soils (1, 2, and 4 mg·kg−1) were investigated.ResultsThe results showed that both cotton straw biochar and compound Bacillus biofertilizer could improve the soil chemical characteristics, including the increase of soil C/N ratio, electrical conductance (EC) and pH, and the most important decrease of soil available Cd content by 60.24% and 74.34%, respectively (P < 0.05). On the other hand, adding cotton straw biochar and compound Bacillus biofertilizer in Cd stressed soil also improved soil biological characteristics. Among them, cotton straw biochar mainly through increasing soil alkaline phosphatase activity and improve bacteria abundance, compound Bacillus biofertilizer by increasing soil invertase, alkaline phosphatase, catalase, and urease activity increased bacterial community diversity. On the whole, the decrease of soil available Cd was mainly caused by the increase of soil pH, C/N, urease and alkaline phosphatase activities, and the relative abundance of Acidobacteria and Proteobacteria.ConclusionsIn summary, the applications of cotton straw biochar and compound Bacillus biofertilizer could decrease soil available Cd concentration, increase soil bacterial community diversity and functions metabolism, and reduce the damage of Cd stress, compared with cotton straw biochar, compound Bacillus biofertilizer was more effective in immobilizing Cd and improving soil environmental quality.

Plastic film mulching with ridge planting alters soil chemical and biological properties to increase potato yields in semiarid Northwest China

BackgroundThe growth of potato (Solanum tuberosum L.) is severely affected by the complex and variable soil environment, and film mulching has been widely used for potato growth in semiarid areas of western China. However, there are few studies on the effects of film mulching on soil quality and tuber yield in potato fields.MethodsThe effects of four mulching patterns (flat plot without film mulching, FP; flat plot with film mulching, FPM; ridge planting with half mulch, RPHM; ridge planting with full mulch, RPFM) on soil chemical and biological properties and potato tuber yield were investigated during two growing seasons (2018 and 2019) in the field.ResultsThe results showed that compared with FP, the mulching treatments significantly increased the tuber yield of potato, with an increase of 3.7–20.77% and 7.89–26.35% in 2018 and 2019, respectively, and the yield of RPFM was higher than that of other treatments. In both growing seasons, RPFM significantly increased the contents of alkali-hydrolyzed N and available P, and the activities of soil urease, catalase, alkaline phosphatase, and sucrase. Bacterial and actinomycete counts were significantly higher in RPFM than those in the other treatments. Furthermore, RPFM significantly increased large potatoes and decreased small potatoes. Pearson’s correlation analysis revealed that soil alkaline dissolved nitrogen and actinomycete populations were the main factors affecting potato yield formation.ConclusionsThese results indicate that RPFM can improve the soil environment and further increase potato tuber yield, which is a viable option for potato production in semiarid areas.Graphical

A comprehensive study on the exposure of nanoplastics to constructed wetland ecological systems: Macrophyte physiology and microbial enzymology, community composition and metabolic functions

The increasing emissions of nanoplastics inevitably pose a potential threat to constructed wetland (CWs) ecosystems. However, to our knowledge, the studies on the impact of nanoplastics on CWs were mainly for the effect on nitrogen removal, but comprehensive research on wetlands is still lacking. Thus, we revealed the overall effects of polystyrene nanoparticles (PSNPs) on wastewater treatment performance, and separate exploration of macrophytes and microbes. Results showed that although 1 and 10 mg/L PSNPs had little influences on COD removal, they caused serious suppression in TN removal, and 10 mg/L PSNPs negatively affected the NH4+-N and TP removal. These suppressions were due to the accumulation of>97% PSNPs attached to the sand biofilm, which proved that CWs could effectively remove nanoplastics. As for macrophyte physiology, PSNPs significantly affected the activities of superoxide dismutase, catalase, peroxidase, and the content of malondialdehyde in each phase. The major photosynthetic pigment biosynthesis was inhibited with 10 mg/L PSNPs. Furthermore, the soil catalase activities and the relative abundances of main antioxidant enzymes were obviously impacted. Caused by excess nanoplastic-induced ROS, almost all activities of dehydrogenase, phosphatase, ammonia monooxygenase, nitrate reductase were significantly inhibited. As for metagenomics analysis, taxonomy annotation revealed that PSNPs negatively affected the major functional bacteria involved in global geochemical nitrogen, phosphorus and sulfur cycle. Functional annotation analysis revealed that some indispensable metabolism pathways (oxidative phosphorylation, pyruvate metabolism, ABC transporter, two-component system, biofilm formation, etc.) were also inhibited. Additionally, nanoplastics reduced the overall pattern of genes related to nitrogen metabolism and partial genes in glucose degradation. Overall, our results comprehensively indicate that nanoplastic is a potential danger to wetland ecosystems and should receive more attention.

Effect of Different Agricultural Farming Practices on Microbial Biomass and Enzyme Activities of Celery Growing Field Soil.

Soil quality is directly affected by alterations in its microbiological, biological, biochemical, physical, and chemical aspects. The microbiological activities of soil can affect soil fertility and plant growth because it can speed up the cycling of nutrients, enzymes, and hormones that are needed by plants for proper growth and development. The use of different agricultural management practices can influence microbial biomass and enzyme activities by altering soil microclimate, soil microorganism habitat, and nutrient cycling. Based on this, the present work planned to evaluate the impact of conventional, low-input, and organic farming systems in a vegetable field growing celery on microbial biomass and different soil enzyme activities. The present study showed a comparison of the effect of different practices on biological soil quality indicators during two sampling times, i.e., one month after colonization and one month before harvesting. It was observed that the soil microbial biomass in the organic farming system was significantly higher than that found in conventional and low-input practices. Under an organic farming system, the soil microbial biomass in December was significantly higher than that in October. The soil microbial biomass carbon in the 0–20 cm soil layer showed higher variation compared to that in the 20–40 cm layer for all the three of the farming management practices that were used in the study. Additionally, the soil total carbon and total organic carbon were recorded as being higher in the December samples than they were in the October samples. Under all the three of the management practices that were applied, the soil catalase activity was higher in the October samples than it was in the December soil samples that were collected the from 20–40 cm soil layer compared to those that were taken from the 0–20 cm layer. The application of organic fertilizer (chicken and cowmanure compost) resulted inincreases in the soil urease and in the protease activity. The protease activity of the soil samples that were extracted from the 0–20 cm and 20–40 cm soil layers in October was higher in the samples that were taken from farms using conventional practices than it was in the samples that were taken from farms using organic and low-input practices, while the samples that were collected during December from both of the soil layers showed higher protease activity when organic methods had been used. No significant variation in the soil urease activity was observed between the two soil layer samples. Urease activity was the highest when organic management practices were being used, followed by the low-input and the conventional modes. For the conventional and low-input practices, the soil urease activity showed an obvious trend of change that was related to thetime of sampling, i.e., activity in December was significantly higher than activity in October. The novelty of this study was to determine the microbial biomass carbon and enzymatic activity in a six-field crop rotation (tomato, cucumber, celery, fennel, cauliflower, and eggplant) using three management practices: low-input, conventional, and organic systems. The present study showed that the long-term application of organic fertilizers plays a large role in maintaining excellent microbial and enzyme activitythat result in improved soil quality.

Effects of irrigation and fertilization on different potato varieties growth, yield and resources use efficiency in the Northwest China

The scarcity of irrigation water has now become the main constraint for crop production globally. Potato growth in arid and semiarid regions is facing increasing challenges due to water deficit. Potato productivity is generally influenced by water and fertilizer, and the requirements for these factors vary depending on the soil type and potato variety. Field experiments were conducted during 2019–2020 to select the suitable potato varieties with the best combination of irrigation and fertilization levels. The three irrigation amounts were W1 (100% crop evapotranspiration (ETC)), W2 (80% ETC) and W3 (60% ETC). The three fertilizer rates were F1 (N–P2O5–K2O, 240–120–300 kg ha−1), F2 (180–90–225 kg ha−1) and F3 (120–60–150 kg ha−1). The three locally cultivated potato varieties were V1 (Feiurita), V2 (Xiapoti), and V3 (Yushu 4). The results showed that irrigation amount, fertilizer amount and potato variety had significant effects on potato growth, yield, quality and water and fertilizer productivity. The average potato yield and irrigation water use efficiency (IWUE) in Feiurita were 12.5% (11.9%) and 14.2% (13.3%) higher than those in Xiapoti in 2019 (2020), respectively. On average, the tuber yield and partial factor productivity (PFP) in W1 were 27.8% (35%) and 26.8% (34.7%) higher than those in W3 in 2019 (2020), respectively. Tuber yield, IWUE and polyphenol oxidase activity (POA) were most subject to irrigation amount, followed by potato variety and fertilizer rate. Potato tuber yield was significantly correlated with soil available potassium, soil actinomycete quantity, soil catalase activity, and soil sucrase activity (SSA). The W2F3V1 treatment had the highest PFP, starch content, reducing sugar content, and vitamin C content (VC), and the tuber yield and crude protein content were relatively high. The W3F2V1 treatment had the highest IWUE and vitamin C, and the starch content and POA were relatively high. Among them, the soil actinomycete quantity, soil cellulose decomposition bacteria quantity (SCDBQ), soil catalase activity, soil urease activity (SUA), and SSA in the W2F3V1 and W3F2V1 treatments were moderate. The W2F3V1 and W3F2V1 treatments are recommended as the best water and fertilizer management in sandy loam soils in Northwest China.

Lowered Cd toxicity, uptake and expression of metal transporter genes in maize plant by ACC deaminase-producing bacteria Achromobacter sp.

In this study, an ACC deaminase-producing bacterial strain Achromobacter sp. A1 was isolated from maize rhizosphere soil, characterized and evaluated for the effects on cadmium (Cd) immobilization in solution/rhizosphere, physiological characteristics and the tissue Cd contents in maize and the molecular mechanisms involved by hydroponic and pot experiments. ACC deaminase activity of strain A1 was significantly enhanced by Cd addition and Cd concentration decreased (55.54–63.62%) in solution supplemented with various Cd concentrations. Strain A1 significantly increased the maize dry weights (30.77–105%) and chlorophyll content (7.46–14.46%), decreased MDA content (25.16–36.87%) and ethylene production (20.93–35.86%) in hydroponic experiment. Strain A1 significantly reduced the above-ground tissue Cd uptake by 12.64–33.68% and 42–48% in hydroponic and pot experiments, reduced the DTPA-extractable Cd content and elevated invertase, urease and catalase activity in rhizosphere soils. In addition, the expression levels of Cd transporter genes HMA3 and Nramp5 were significantly reduced in root and shoot after strain A1 inoculation. These results indicate that strain A1 has great potential for application as a novel and environmentally friendly inoculant to immobilize Cd and reduce maize Cd uptake in Cd-contaminated environments, and will improve the understanding of the relative molecular mechanisms underlying the response to strain A1 in maize plant.

Changes of soil microbial biomass carbon, nitrogen, and enzyme activities in East Dongting Lake wetlands at different water levels

We analyzed soil quality based on soil microbial characteristics of three different vegetation types in the wetlands of East Dongting Lake, including Carex tristachya wetland (CTW), Phragmites australis wetland (PAW), and Salix babylonica wetland (SBW). The soil microbial biomass carbon (MBC), nitrogen (MBN) and enzyme activities were measured and the key influen-cing factors were analyzed during the normal, flood, and dry periods. The results showed that: 1) The amounts of MBC, MBN, and the activities of invertase and cellulase (except cellulase of dry season) in 0-10 cm were higher than those in 10-20 cm for all wetlands, while the catalase activity showed an opposite pattern. 2) The amounts of MBC and MBN and the values of MBC/TOC and MBN/TN for the 0-20 cm soil layer of each vegetation type wetland were the lowest in flood period. 3) Soil invertase activity for each vegetation type wetland in the 0-20 cm soil layer peaked in the dry period, while soil cellulase activity peaked in the normal period. The seasonal fluctuation of soil catalase activities in all wetlands were small, with activities being slightly higher in flood period than the other two periods. 4) Among different vegetation types, soil invertase activity of PAW was significantly higher than that of other vegetation types, and cellulase activity of which was the lowest in both normal and flood periods. There was no difference in these two enzymes activities among wetlands during the dry period. The highest soil catalase activity was found in CTW during normal period and in SBW during dry period, respectively, while its lowest value was in PAW during flood period. 5) Soil MBC, MBN and invertase activity were correlated positively with soil TOC, TN and TP, and negatively correlated with soil pH. The activities of soil cellulase and catalase were significantly negatively correlated with TOC, TN, TP and positively correlated with pH. It suggested that the seasonal fluctuation of water level affected soil C, N, P contents and pH values, with consequences on soil MBC, MBN and enzyme activities.

Does Complex Soil Enhance Grain Yield under Cropping System?

Global climate change and the increasing population have increased the difficulties associated with grain production. Several measures have been established to maintain a high crop yield, while preserving or increasing soil health, including biochar application to soil, and producing new complex soil with soil amendment application, e.g., biochar and soft rocks. However, previous studies have focused on the effect of complex soil on a single crop but have not considered crop rotation. In this study, field plots with foxtail millet cultivated for two years under biochar and soft rock application were selected for licorice cultivation to detect the effects of biochar and soft rock application on soil properties and licorice yield. The results showed that the biochar-treated plot had the highest licorice biomass (251.76 g/m2), followed by the combined biochar and soft rock treatment, and that the soft rock and control treatments had the lowest licorice biomass (97.65 g/m2). Plants in biochar-treated plots had the highest liquiritin and glycyrrhizic acid contents, followed by those under soft rock treatment. Soft rocks and biochar increased the soil catalase activity, organic matter, oxalic acid, tartaric acid, formic acid, and available phosphorus (AP). Correlation analysis showed that the licorice biomass was significantly positively correlated with oxalic acid and AP and negatively correlated with soil pH. AP was positively correlated with catalase activity and oxalic acid (p &lt; 0.05) and negatively correlated with soil pH (p &lt; 0.05). Therefore, it can be concluded that the addition of biochar and soft rocks for two years could increase soil organic acid contents (especially that of oxalic acid), which function to reduce soil pH, increase soil AP content, and enhance licorice biomass.

Effects of Vegetation Restoration on Soil Enzyme Activity in Copper and Coal Mining Areas.

Mining areas are suffering from serious environmental hazards, such as soil erosion, water pollution as well as land degradation. In this study, two types of mining areas in Anhui Province, China-one a copper mining area and the other a coal mining area-were selected to compare the soil properties under different vegetation restoration conditions, which can be generally classified into reclaimed and non-reclaimed areas. Soil catalase and urease activities and soil chemical properties were chosen to be the main indicators of soil quality. Principal component analysis was used to evaluate the overall soil fertility in the copper and coal mining areas. Results showed that in the copper mining area soil catalase activity was between 12.36 and 19.17 μg g-1 h-1 and urease activity was between 0.03 and 12.05 μg g-1 h-1. And in coal mining area, soil catalase activity was between 3.52 and 9.72 μg g-1 h-1 and urease activity was between 2.71 and 10.81 μg g-1 h-1. Moreover, soil catalase and urease activities in degraded areas were lower than those in reclaimed areas. Soil catalase activity and soil urease activity were significantly correlated with total potassium and total nitrogen, respectively. Soil quality in land types with vegetation restoration was higher than in non-reclaimed areas and old subsidence areas, while soil quality in the copper mining area was generally higher than in the coal mining area. Thus, the optimum measure in this region to ameliorate these degraded soils is vegetation restoration, which helps not only to improve the environment, but also to enhance soil quality in these degraded lands.

Heavy metal fixation of lead-contaminated soil using Morchella mycelium

With the exploitation of lead-zinc deposits, lead content around mining areas has seriously exceeded the recommended level. The most challenging problem is how to reduce lead contamination in soil efficiently. In this study, we developed a method to remediate lead-contaminated soil by adding Morchella mycelium. First, we compared the repair effects of mycelium and hyperaccumulator by conducting pot experiments. Then, we investigated the mechanism through which mycelium repairs lead-contaminated soil by conducting simulation experiments. Results showed that using mycelium was a more efficient way to repair soil than using hyperaccumulator. Compared with the untreated group, mycelium reduced the lead content of crops by 34.83 % and raised dry biomass by 134.05 % when lead addition was 800 mg/kg. After mycelium fixation, soil catalase, urease, cellulase, and sucrase activities were significantly enhanced, and the bioavailability of lead decreased significantly. The lead solution exposure simulation test showed that Morchella mycelium immobilized lead due to its extracellular secretions. That is, mycelium secreted metabolites and lead to form salt crystals, reducing bioavailable lead content. In addition, Morchella mycelium restoration may effectively improve soil fertility and increase crop yields. Thus, mycelium may be used successfully in alternative green repair methods for environmental heavy metal remediation.

Influences of different source microplastics with different particle sizes and application rates on soil properties and growth of Chinese cabbage (Brassica chinensis L.)

The potentially negative effects of microplastics (MP) on agroecosystems have raised worldwide concerns. However, little is known about the negative effects of MP exposure on the soil–plant system. To fill up this knowledge gap, a pot experiment was set up, and two different MP types [high density polyethylene (HDPE) and general purpose polystyrene (GPPS)] were used, which had four particle sizes (<25, 25–48, 48–150, and 150–850 µm) at four application rates (2.5, 5, 10, and 20 g MP kg−1 soil). Some soil properties and the growth of Chinese cabbage (Brassica chinensis L.) were monitored. The results showed that (1) MP application with high application rates and relatively small particle sizes significantly enhanced the soil urease activity, which accompanied with enhanced soil pH and decreased soil available concentrations of phosphorus and potassium in some cases. (2) The exposure of MP did not significantly affect the activity of soil catalase regardless of their application rates and sizes. MP with different application rates and small sizes significantly reduced the soil sucrase activity, but the largest size of MP enhanced the activity of soil sucrase. (3) GPPS at 10–20 g kg−1 or with the sizes of <25 and 48–150 µm significantly reduced the fresh weight of Chinese cabbage, but the addition of HDPE had no remarkable effects on the fresh weight regarding of its application rates or sizes. (4) MP with high application rates and large sizes enhanced but small sizes of MP reduced the leaf soluble sugar concentration. The increasing application rates of MP and small size HDPE significantly reduced the starch concentration in the leaves of Chinese cabbage, however, the different sizes of GPPS showed limited effects on the leaf starch. The addition of MP with increasing application rates and different sizes always reduced the concentration of leaf chlorophyll. These parameters regarding to plant and soil could be used to assess the risks of MP pollution in the soil–plant systems. We found that the risks resulting from MP pollution were MP type-dependent and particle size-dependent. These findings indicate that overaccumulation of MP in the agriculture may possess an ecology risk and will negatively affect the agricultural sustainability and the food safety.

Effects of tetracycline antibiotics in chicken manure on soil microbes and antibiotic resistance genes (ARGs).

China is the world's largest livestock and poultry breeding country, but also the largest use of veterinary antibiotics. When a large amount of chicken manure is applied to the soil, it will cause the number of antibiotic residues and resistant bacteria to increase, which will bring about the pollution of antibiotic resistance genes (ARGs) in the soil, and then increase the risk of environmental pollution and human health. Field experiments were conducted to study the changes of soil tetracycline antibiotic residues, resistant bacteria and resistance genes treated with different types and dosage of chicken manure (no chicken manure, (CK), low fresh chicken manure treatment (300kg·667m-2), high fresh chicken manure treatment (600kg·667m-2), low decomposed chicken manure treatment (300kg·667m-2) and high decomposed chicken manure treatment (600kg·667m-2)). After one-year application of chicken manure, content of soil organic matter increased by 1.0%-3.2% compared with the control. The activity of soil catalase significantly increased by 84.3-91.5%, 81.9-102.9% in fresh and decomposed chicken manure treatments compared with the control, respectively. The amount of soil resistant bacteria under the same treatment was in the order of Anti-OTC > Anti-TC > Anti-CTC. After one-year application of chicken manure, the total tetracycline amount in the soil was increased by 168.5-217.9% compared with the control. The amount of antibiotic residue in soil treated with fresh chicken manure was 3.0-9.1% higher than that treated with decomposed chicken manure. The abundance of ARGs in the soil was in the order of that treated with high fresh chicken manure > low fresh chicken manure > high decomposed chicken manure > low decomposed chicken manure. The risk of tetracycline antibiotics to soil ecological environment may be greatly reduced after chicken manure decomposed.

Carbon Mineralization Dynamics of Organic Materials and Their Usage in the Restoration of Degraded Tropical Tea-Growing Soil

Understanding carbon mineralization dynamics of organic amendments is essential to restore degraded lands. This study focused on the restoration potentials of tea-growing soils using organic materials available in tea ecosystems. The Selangor-Briah soil series association (Typic Endoaquepts) consisted of a high- (soil A) and a low-carbon (soil B) soils were incubated with different organic materials and released carbon dioxide (CO2) measured. Two kinetic models were applied to depict the mineralization process. Soil health parameters including microbial biomass carbon and nitrogen, dehydrogenase and catalase activities were determined to assess the restoration potentials. The parallel first-order kinetic model fitted well for all amendments. Gliricidia markedly enhanced the net cumulative CO2 flux in both soils. Charged biochar, tea waste and Gliricidia improved the microbial biomass carbon by 79–84% in soil A and 82–93% in soil B, respectively. Microbial quotients and biomass nitrogen were increased over 50 and 70% in amended soils, respectively. Dehydrogenase activity was significantly accelerated over 80% by compost, charged biochar and tea waste. Charged biochar remarkably increased the soil catalase activity by 141%. Microbial biomass, dehydrogenase and catalase activities, and cumulative CO2 flux were positively correlated (r &gt; 0.452) with one another. The studied amendments showed greater potential in improving the soil quality, while charged biochar, raw biochar and compost enrich the soil recalcitrant C pool ensuring the soil health in long term. Even though biochar sequesters carbon, it has to be charged with nutrients to achieve the soil restoration goals.

Surfactant-enhanced treatment of oil-contaminated Arctic tundra soil: Ecotoxicological assessment

During 2018–2019 summer expeditions to the Yamal peninsula, we collected tundra gley soil samples at the oil-contaminated sites. The contamination level was determined by fluorimetry and was ranged from 30 to 107 g/kg. The soil quality was evaluated by its enzymatic activity; the low catalase activity of the soils confirmed the presumed slow soil natural attenuation and the need for land remediation. The oil-contained and background samples were ex-situ treated with the aqueous solutions of non-ionic BOK-6 and anionic AddiMax PV01 synthetic surfactants. The treatment efficiency reached 71 ± 2% and 97 ± 3% in the experimental design with BOK-6 and AddiMax PV01, respectively. We derived the ecotoxicity endpoints for the surfactant solutions, using infusoria Paramecium caudatum Ehrenberg and higher plants Raphanus sativus L. and Avena sativa L. The protozoan mortality was studied and resulted in LC 50 − 24 values << 0.1 mg/L and 339 mg/L for BOK-6 and AddiMax PV01, respectively. Both surfactant solutions and surfactant residues in soil showed the inhibition potential towards higher plants. BOK-6, with a strong relation to its chemical and ecotoxic profile, completely inhibited seed germination in the treated soil. The less ecotoxic surfactant AddiMax PV01 inhibited plant growth by 38% compared to control. The treatment increased ecotoxicity and dehydrogenase activity and further lowered the catalase activity of the background soil, and, therefore, the surfactant residues migrating to the non-contaminated territories should be controlled. By this study, we show the efficiency of the surfactant-enhanced remediation of arctic soils, but the associated environmental risks must be addressed. • Ex-situ treatment of oil-contaminated tundra gley soil reached 69%–99% efficiency. • Enzymes activity indicated slow natural attenuation potential of arctic soil. • Residues of toxic surfactant could cause greater ecotoxic effect than contamination. • Surfactants residues inhibited biological activity of non-contaminated arctic soil.

Bio-activation of soil with beneficial microbes after soil fumigation reduces soil-borne pathogens and increases tomato yield

Soil-borne diseases have become increasingly problematic for farmers producing crops intensively under protected agriculture. Although soil fumigants are convenient and effective for minimizing the impact of soil-borne disease, they are most often detrimental to beneficial soil microorganisms. Previous research showed that bio-activation of soil using biological control agents present in biofertilizers or organic fertilizers offered promise as a strategy for controlling soil-borne pathogens when the soil was bio-activated after fumigation. Our research sought to determine how bio-activation can selectively inhibit pathogens while promoting the recovery of beneficial microbes. We monitored changes in the soil’s physicochemical properties, its microbial community and reductions in soil-borne pathogens. We found that the population density of Fusarium and Phytophthora were significantly reduced and tomato yield was significantly increased when the soil was bio-activated. Soil pH and soil catalase activity were significantly increased, and the soil’s microbial community structure was changed, which may have enhanced the soil’s ability to reduce Fusarium and Phytophthora. Our results showed that soil microbial diversity and relative abundance of beneficial microorganisms (such as Sphingomonas, Bacillus, Mortierella and Trichoderma) increased shortly after bio-activation of the soil, and were significantly and positively correlated with pathogen suppression. The reduction in pathogens may have been due to a combination of fumigation-fertilizer that reduced pathogens directly, or the indirect effect of an optimized soil microbiome that improved the soil’s non-biological factors (such as soil pH, fertility structure), enhanced the soil’s functional properties and increased tomato yield.

Biological Effect of Tetracycline Antibiotics on a Soil-Lettuce System and Its Migration Degradation Characteristics

Residual tetracycline antibiotics (TCs) in farmland soils with the application of livestock manure cause risks to the growth of vegetables and soil ecology. Here, pot experiments are carried out using through exogenous addition of different levels of oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC), to study the physiological toxicity, uptake, and transportation of TCs in lettuce. The subsequent degradation of TCs in soil was also evaluated along with analyses of soil enzyme activity and microbial population dynamics. The results showed that the biomass of lettuce decreased with application of TCs as well as the chlorophyll-a, chlorophyll-b, and carotenoid content. Consequently, net photosynthetic rates were inhibited, and SOD, POD, and CAT increased under the stress imposed by the TCs. With an increase in the level of TC application, uptake by lettuce plants increased while the bioconcentration and translocation factors decreased. When OTC, TC, and CTC in the soil were below 150 mg·kg-1, the health risk from the edible parts of lettuce was low (HQ<0.1). The TC degradation rate in different soils was ranked in the order of control soil > rhizosphere soil > bulk soil. The OTC degradation rates in the soils were significantly lower than for TC and CTC. TCs (150-1350 mg·kg-1) significantly inhibited urease and rhizosphere catalase activity in soil and reduced the number of soil culturable bacteria and fungi.

Оксидоредуктазна активність ґрунтів в умовах нафтового забруднення та фіторемедіації

The effect of oil pollution and phytoremediation with Zea mays L. and Vicia faba var. Minor on soil oxidoreductase activity was investigated. The experiment was carried out under field conditions. Soil was artificially contaminated by oil at rate 4.2 % (50 ml of oil per 1 kg of soil). Soil sampling was performed at 10th, 22th, 30th days (the firs experiment stage) before sowing plants and seedlings emergence and at 65th, 95th days (second experiment stage) with plants vegetation. Results showed that soil catalase activity (CA) was reduced during the first experiment stage and increased later under oil contamination conditions compared to control. The reduction of soil enzyme activity can be related to inhibition of soil microbial activity by hydrocarbons toxic influence. On the other hand recovery of enzyme activity can be due to decrease of toxicity of soil conditions over time. A two-way analysis of variance yielded a main effect for the phytoremediation (p&lt;0,01), such that the average CA was significantly higher for soil with plants than for unplanted soil. The interaction effect was significant between oil contamination and phytoremediation with Z. mays (p&lt;0,01), indicating that the plants’ effect was greater in the oil contamination condition than without oil contamination. Soil dehydrogenase activity (DA) in oil contamination conditions decreased during all experiment compared to control. A main effect of phytoremediation on DA was significant (p&lt;0,05), the interaction effect between oil contamination and phytoremediation was significant too (p&lt;0,05). Interaction effect between oil contamination and phytoremediation can indicate improving conditions for microbial activity in the rooting zone. The plant’s exudates may create more feasible conditions for utilisation of carbohydrates by microorganisms. Polyphenol oxidase (PPOA) activity decreased on the 95th day in oil contaminated soil compared to control. PPOA was stimulated by Z. mays and V. faba plants in soil without oil, and only by Z. mays in oil contaminated soil. Soil phytoremediation treatment was related to the increase of enzyme activity. Such plants can be feasible for oil contaminated soil remediation.