Activity Of Different Essential Oils Research Articles

In Vitro Virucidal Activity of Different Essential Oils against Bovine Viral Diarrhea Virus Used as Surrogate of Human Hepatitis C Virus.

The hepatitis C virus (HCV) is a major hepatotropic virus that affects humans with increased risk of developing hepatocellular carcinoma. The bovine viral diarrhea virus (BVDV) causes abortion, calf mortality and poor reproductive performance in cattle. Due the difficulties of in vitro cultivation for HCV, BVDV has been used as surrogate for in vitro assessment of the efficacy of antivirals. Essential oils (EOs) display antiviral and virucidal activity on several viral pathogens. In this study, the virucidal activity of five EOs, Salvia officinalis L. EO (SEO), Melissa officinalis L. EO (MEO), Citrus lemon EO (LEO), Rosmarinus officinalis L. EO (REO) and Thymus vulgaris L. EO (TEO) against BVDV was evaluated in vitro at different concentrations for several time contacts. MEO and LEO were able to considerably inactivate BVDV with a time- and dose-dependent fashion. MEO and LEO at the highest concentrations decreased viral titer by 2.00 and 2.25 log10 TCID50/50 μL at 8 h contact time, respectively. SEO, REO and TEO displayed mild virucidal activity at the highest concentrations for 8 h contact times. In this study, the virucidal efficacies of MEO and LEO against BVDV were observed regardless of compound concentration and contact time. Further studies are needed to confirm the potential use of MEO and LEO as surface disinfectants.

Chemical Composition and Biocontrol Activity of Different Essential Oils against Soil-Borne Fungal Pathogens.

In this study, the efficacy of the essential oil of Mentha longifolia, Achillea arabica and Artemisia absinthium plants were evaluated against important soil-borne fungal pathogens as Verticillium dahliae, Rhizoctonia solani, and Fusarium oxysporum. Essential oils were obtained from plants by hydrodistillation method and the chemical components of essential oils were determined by analyzing by gas chromatography-mass spectrometry. The main components found as piperitone oxide (13.61%), piperitenone oxide (15.55%), pulegone (12.47%), 1-menthone (5.75%), and camphor (5.75%) in M. longifolia, á-selinene 13.38%, camphor 13.34%, L-4-terpineneol 8.40%, (-)-á-Elemene 7.01%, 1,8-cineole 4.71%, and (-)-spathulenol 3.84% in A. arabica, and á-thujone (34.64%), 1,8-cineole (19.54%), pulegone (7.86%), camphene (5.31%), sabinene (4.86%), and germacrene-d (3.67%) in A. absinthium. The antifungal activities of the oils were investigated 0.05, 0.1, 0.25, 0.5, 1.00, and 2.00 μl/ml concentrations with the contact effect method. M. longifolia oil (1.00 and 2.00 μl/ml) has displayed remarkable antifungal effect and provided 100% inhibition on mycelial growth of V. dahliae, R. solani and F. oxysporum. The results obtained from this study may contribute to the development of new alternative and safe methods against soil-borne fungal pathogens.

Antifungal Activities of Different Essential Oils and Their Electrospun Nanofibers against Aspergillus and Penicillium Species Isolated from Bread.

Mold growth, especially Aspergillus spp. and Penicillium spp., deteriorates the quality of bakery products. Essential oils (EOs) have been categorized as good natural antimicrobials. Hereby, this study aimed to evaluate the antifungal activity of six EOs, ginger, cumin, cinnamon, black pepper, origanum, and clove, and their volatile compounds against fungal strains isolated from bread: Penicillium carneum DDS4, Aspergillus flavus DDS6, and Aspergillus niger DDS7 by disc diffusion and disc volatilization methods, respectively. Among EOs, cumin, cinnamon, origanum, and clove were found to be effective against fungal strains, and their minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined. The observed lowest MIC value of EOs was obtained at 1000 μg/mL concentration, and the lowest MFC value was obtained from the results of clove at a concentration of 1000 μg/mL. Based on the MIC and MFC values, clove and cinnamon EOs were found to be more effective at lower concentrations. Electrospun nanofiber films of clove and cinnamon were produced with 6% poly(vinyl alcohol) (PVA), 2% β-cyclodextrin (β-CD), and 2% EO to overcome the unfavorable sensory impact of EOs on food products. The inhibitory activity of cinnamon EO film (2.64–2.51 log(CFU/mg)) was considerably lower than clove EO film (3.18–3.24 log(CFU/mg)) against P. carneum DDS4 and A. niger DDS7. Furthermore, these nanofiber films prevented fungal growth on bread samples visibly and were shown to be an alternative application for active food packaging.

Assessment of the antimicrobial activity of some essential oils against multi-resistant bacterial strains

Volatile oils, also called essential oils, are secreted by plant-specific secretory cells and tissues. These components are a mixture of different substances, primarily mono and sesquiterpenes. Our aim was to assess the antimicrobial activity of different essential oils, i.e. lavender, cloves, eucalyptus, rosemary, thyme and oregano against multidrug resistant bacterial strains belonging to the ESKAPE group. The most effective oils proved to be the thyme and oregano which induced the occurrence of the largest diameters of the growth inhibition zones against all tested bacterial species. Oregano oil showed the best inhibitory activity against Pseudomonas aeruginosa, followed by clove, while thyme oil was the most effective against Staphylococcus aureus strains.

Bioactivity of Essential Oils for Mitigation of Listeria monocytogenes Isolated from Fresh Retail Chicken Meat.

Listeria monocytogenes is one of the most severe foodborne pathogens found in several habitats. Therefore, this study aims to investigate the antilisterial activity of different essential oils (EOs) against multidrug-resistant (MDR) L. monocytogenes strains isolated from fresh chicken meat. Our results showed that the prevalence of L. monocytogenes in the examined samples was 48%. Seventy-eight isolates were identified as L. monocytogenes. Out of these, 64.1% were categorized as MDR and were categorized in 18 patterns with 50 MDR isolates. One isolate was selected randomly from each pattern to investigate their biofilm-forming ability, resistance, and virulence genes incidence. Out of 18 MDR isolates, 88.9% showed biofilm-forming ability. Moreover, the most prevalent resistance genes were ermB (72%), aadA (67%), penA (61%), and floR genes (61%). However, the most prevalent virulence genes were inlA (94.4%), prfA (88.9%), plcB (83.3%), and actaA (83.3%). The antilisterial activity of EOs showed that cinnamon bark oil (CBO) was the most effective antilisterial agent. CBO activity could be attributed to the bioactivity of cinnamaldehyde which effects cell viability by increasing the bacterial cell electrical conductivity, ion leakage, and salt tolerance capacity loss. Therefore, CBO could be an effective alternative natural agent for food safety applications.

Adaptogenic activity of Cinnamomum camphora, Eucalyptus globulus, Lavandula stœchas and Rosmarinus officinalis essential oil used in North-African folk medicine.

Depressive anxiety is one of the most emotional disorders in our industrial societies. Many treatments of phobias exist and are based on plant extracts therapies, which play an important role in the amelioration of the behavior. Our study aimed to evaluate the adaptogenic activity of different essential oils provided from local plants: Cinnamomum camphora (Camphora), Eucalyptus globulus (Blue gum), Lavandula stœchas (Topped lavender) and Rosmarinus officinalis (Rosemary) on Wistar rats. The adaptogenic activity was evaluated on the elevated plus-maze. The efficacy of the extract (200 mL/kg) was compared with the standard anxiolytic drug Diazepam® 1 mg. Animals administered by the essential oil of Lavandula stœchas, Cinnamomum camphora, Rosmarinus officinalis and Eucalyptus globulus showed a behavior similar to those treated with Diazepam®. For groups treated with the following essential oils: Rosmarinus officinalis, Lavandula stoechas and Cinnamomum camphora at a dose of 200 mL/kg, we notice an increase in the time spent on the open arms of the elevated plus-maze and a decrease in time spent on the closed arms of the elevated plus-maze, especially for Rosmarinus officinalis, which explains the anxiolytic effect of these plants. We also notice a decrease in the number of entries in closed arms, open arms and the number of passing to the central square. The increase in the number of entries to open arms with Eucalyptus globulus essential oil shows a reduction in anxiety behavior in rodents and this shows that these plants have an inhibitory effect.

Relationship between Chemical Composition and Nematicidal Activity of Different Essential Oils.

In this study, the relationship between nematicidal activity and chemical composition of ten essential oils (EOs) from different plant species was investigated both in in vitro assays on juveniles (J2) and eggs of the root-knot nematode Meloidogyne incognita and in experiments on tomato in soil infested by M. incognita. Nematode J2 were exposed for 4, 8 or 24 h to 0.78–100 μg mL−1 concentrations of each EO, whereas 24, 48 or 96 h exposures to 250, 500 and 1000 μg mL−1 solutions were tested on M. incognita egg masses. Treatments with 50, 100 or 200 μg kg soil rates of each EO were applied in the experiment on potted tomato. The highest nematicidal potential resulted for the C. verum EO, as highly toxic to both M. incognita J2 and eggs and strongly suppressive on nematode multiplication on tomato roots. The infestation of M. incognita on tomato roots was also strongly reduced by the EOs from E. citriodora and S. aromaticum, both highly toxic to M. incognita J2 but less active on nematode eggs. Adversely, R. graveolens EO strongly inhibited the egg hatch but was limitedly toxic to the infective J2. Chemical composition of the EOs was determined by GC-FID and GC-MS. The ten EOs showed a very different chemical composition in terms of major phytochemicals, with one or two dominant components totally amounting up to 85%. The structure–activity relationship based on the main phytochemicals identified in the assayed EOs and their nematicidal effects on M. incognita was also discussed. Results from this study confirmed that the selection of suitable EO raw materials can lead to the formulation on new effective nematicidal products.

Phytochemical Composition and In Vitro Antimicrobial Activity of Essential Oils from the Lamiaceae Family against Streptococcus agalactiae and Candida albicans Biofilms.

The antimicrobial activity of different essential oils (EOs) from the Lamiaceae family was evaluated on Streptococcus agalactiae, Candida albicans, and lactobacilli. S. agalactiae is the main cause of severe neonatal infections, such as sepsis, meningitis, and pneumonia. C. albicans is a primary causative agent of vulvovaginal candidiasis, a multifactorial infectious disease of the lower female reproductive tract. Lactobacilli represent the dominant bacterial species of the vaginal flora and constitute the natural defense against pathogens. On the basis of the preliminary results, the attention was focused on the EOs from Lavandula x intermedia Emeric ex Loisel. and Mentha arvensis L. By using gas ghromatography (GS) retention data and mass spectra, it was possible to identify more than 90% of the total composition of the EO samples. The minimal inhibitory concentration (MIC) and anti-biofilm activity of the two EOs were determined against all isolated strains, using the EOs by themselves or in combination with each other and with drugs (erythromycin and fluconazole). The results showed a good antimicrobial and anti-biofilm activity of both EOs and a synergistic effect, leading to the best results against all the strains, resulted using the combinations EOs/EOs and antimicrobials/EOs.

Determination of Antimicrobial Activity of Different Essential Oils Obtained from Plants on Staphylococcus aureus Strains Isolated from Foods

Essential oils (EOs) are known for its antimicrobial activity against several pathogenic bacteria. The present work evaluated the antimicrobial activity of 15 different EOs on survival of different strains of different Staphyloccocus aureus strains isolated from traditional cheeses by disc diffusion method. The most antimicrobial activity on the strains was found as oil thyme oil (mean zone diameter 23.203 mm). Clove oil and black seed oil had the highest antimicrobial activity after thyme oil with average zone diameters of 13.698 mm and 11.267 mm respectively. Hypericum perforatum L. oil (mean zone 6.209 mm), ginger oil (mean zone 6.250 mm) and garlic oil (mean zone 6.267 mm) were the lowest antimicrobial activity. New studies about antimicrobial effect of EOs in vivo conditions are recommended.

Actividad Antimicrobiana de Diversos Aceites Esenciales en Bacterias Benéficas, Patógenas y Alterantes de Alimentos

El presente estudio evaluó la actividad antimicrobiana de diferentes aceites esenciales (limón, naranja, mandarina, laurel, eucalipto y burro) en bacterias benéficas como Lactobacillus plantarum ES147 y ATCC 8014, patógenas como Escherichia coli ATCC 25922 y una bacteria alterante de alimentos, Leuconostoc mesenteroides MS1. En los aceites que se registraron halos de inhibición significativo (al menos 10 mm de halo en 3 cepas analizadas) se procedió a determinar las concentraciones mínimas inhibitoria y bactericida. Los aceites esenciales de limón, eucalipto y burro mostraron un efecto bactericida en todas las cepas analizadas. Los aceites esenciales de naranja y mandarina presentaron muy bajo o nulo poder antimicrobiano en las cepas en estudio. Los aceites esenciales de burro, laurel, mandarina y naranja no inhibieron o inhibieron muy poco a las bacterias benéficas y generaron grandes halos de inhibición en bacterias patógenas o alterantes de alimentos. Dichos aceites pueden considerarse para su adición en films o recubrimientos de alimentos.

Antifungal activity of different essential oils against Malassezia pathogenic species.

Plants produce essential oils in response to physiological stresses, pathogen attacks and ecological factors. Nowadays, they are recognized as defence compounds and attractors of pollinators. Essential oils have been traditionally used in the past years in various cultures for medicinal and health purposes. In recent times due to their well-documented antimicrobial activities, essential oils have consolidated their use in raw and processed food preservation, health and clinical uses. The potential activity of essential oils against the largely diffused Malassezia species on the human skin, which can cause common infections or exacerbate multiple skin disorders, such as P. versicolor, folliculitis, seborrheic dermatitis and dandruff, atopic dermatitis and psoriasis. Information on essential oils activity against Malassezia species was obtained from published materials, including books and electronic databases, such as SCI finder, PubMed, Web of Science, ACS, Science Direct, Wiley, Springer, Taylor, J-STAGE and Google Scholar. Search was conducted covering the period from January 2013 to December 2018. In the in vitro studies diverse methods were used to test the essential oils activity, namely broth microdilution method, which resulted the most used one, followed by agar disk diffusion and vapour phase methods. Essential oils obtained by steam distillation were from different plant genera, Thymus, Artemisia, Malaleuca, Cinnamomun, Ocimum, Zataria, Rosmarinus, Origanum, Syzigium, Foenicolum, Thapsia, Tachyspermum, Myrtus. MIC values were expressed as μg/mL, μL/mL and by inhibition zone (mm) or μL/cm3. All the investigated essential oils were active at the tested conditions. In addition, two clinical studies used essential oils from Cymbopogon citratus and C. flexuosus formulated in shampoo, cream or lotion for the successful treatment of dandruff and P. versicolor. Results of these studies indicate worthy prospects for clinical application of essential oils and there is an urgent need to conduct further in vivo studies with large number of patients in order to verify the clinical potential of essential oils against Malassezia species.

Comparative Study of Antibacterial Activity of Different Essential Oils against Different Bacteria

Comparative Study of Antibacterial Activity of Different Essential Oils against Different Bacteria

Inhibitory effects of cinnamon and thyme essential oils against Salmonella spp. in hummus (chickpea dip)

The antimicrobial activity of different essential oils was screened against five Salmonella serotypes using disk diffusion. Cinnamon (Cinnamomum zeylanicum) and thyme (Thymus vulgaris) essential oils exhibited the highest activity with inhibition zones of 22.5–38.5 mm in diameter. These oils were also investigated for their ability to control Salmonella and mesophilic aerobic bacteria (MAB) in hummus stored at 4 or 10°C. Salmonella cells were not detected in hummus with 0.5%–1.0% cinnamon essential oil by 7 or 1 d, respectively, at both 4 and 10°C. Cinnamon oil at 0.5%–1.5% reduced the MAB in hummus by 1.3–4.6 log CFU/g at 4 or 10°C. However, addition of 0.5%–1.5% thyme essential oil to hummus reduced Salmonella by 1.0–2.9 log CFU/g, at 4°C by 10 d. Thyme oil also inhibited growth of MAB in hummus and the count remained constant until the end of storage at 4°C, but at 10°C, thyme oil was less inhibitory. Practical applications Consumption of hummus has been linked to several salmonellosis outbreaks. Addition of cinnamon and thyme essential oils at concentrations of 0.5% may improve the safety and extend the shelf-life of hummus with acceptable organoleptic properties.

Development and validation of mathematical models for testing antifungal activity of different essential oils against Candida species

ObjectiveThe upward trend in using plant materials introduced essential oils (EOs) as a valuable, novel, bioactive antifungal agent and as an alternative to standard treatment protocol of denture stomatitis caused by Candida species. Therefore, the aim was to evaluate the antifungal activity of different EOs and to present the response surface methodology (RSM) and artificial neural network (ANN) as possible tools for optimizing and predicting EOs antifungal activity. MethodsMinimum inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) of the EOs against 3 species Candida spp. (C. albicans, C. tropicalis, C. glabrata) isolated in patients with DS were determined, together with optimization and prediction based on non-terpene and terpene content in EOs, using two mathematical models RSM and ANN-GA. ResultsThe highest concentrations of EO M. alternifolia inhibited (1.6–2.8 μg/ml) and fungicided (3.5–6.0 μg/ml) all three investigated Candida spp. while the lowest concentrations of EO C. limon inhibited (0.2 – 0.5 μg/ml) and fungicided (0.6–1.1 μg/ml). Among the three types of Candida, C. glabrata was the most sensitive. The RSM modelling proved that MICs and MFCs statistically depend on non-terpene and terpene content in different EOs (<0.0001). Both models showed that a citrus oil (EO C. limon) with 89% content of terpenes and limonene as major constituent was more antifungal efficient. ConclusionsThe investigated EOs showed a broad spectrum of anticandidal activity, also confirmed using the RSM and ANN-GA models. Since EOs can be cytotoxic in higher concentrations, models may be used for qualitative and quantitative dosage predictions of the antifungal activity of EOs.

Fumigant and Repellent Activities of Different Essential Oils Alone and Combined Against the Maize Weevil (Sitophilus Zeamais Motschulsky)

Fil: Arena, Julieta Soledad. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Biologia Vegetal. Universidad Nacional de Cordoba. Facultad de Ciencias Exactas Fisicas y Naturales. Instituto Multidisciplinario de Biologia Vegetal; Argentina

Antimicrobial Activity of Different Essential Oils against Aspergillus flavus and Klebsiella pneumoniae Isolated from Respiratory Tract

ESSENTIAL oils from Cinnamomum verum (Cinnamon), Lavandula angustifoli (Lavender), Lepidium virginicum (Peppergrass oil), Zingiber officinale (Ginger) and Cinnamomum camphora (Camphor) have been investigated for their antifungal and antibacterial activities against 6 fungal isolates of Aspergillus flavus and 25 bacterial isolates of Klebsiella pneumoniae. The sensitivity was determined using agar well diffusion method and the inhibition zones were compared with the standard drug itraconazole for fungi and Amikacine for tested bacteria. The tested essential oil showed a wide spectrum of inhibition against the tested isolates. Treating the Klebsiella pneumoniae, with cinnamon essential oil led to an external changes, irregular cell shape and disintegration of bacterial cell wall under transmission electron microscope. GC-MS technique was used for cinnamon essential oil; the composition of cinnamon oil was dominated by cinnamaldehyde. The in vivo efficacy of cinnamon essential oil in treatment of Klebsiella pneumoniae in mice lungs was proven.

032 Antibacterial activity of different essential oils and their combinations against relevant enteric pathogenic bacteria

032 Antibacterial activity of different essential oils and their combinations against relevant enteric pathogenic bacteria

Essential oils: in vitro activity against Leishmania amazonensis, cytotoxicity and chemical composition.

BackgroundThe current chemotherapy for cutaneous leishmaniosis (CL) has a series of drug limitations such as toxic side effects, long duration, high costs and drug resistance, which requires the development of new drugs or effective alternatives to the CL treatment. Essential oils (EOs) are complex mixtures of secondary metabolites from various plants. It has been shown that several EOs, or their constituents, have inhibitory activity against protozoa. Thus, this study aims to evaluate the biological activity of different essential oils (EOs) on Leishmania (L.) amazonensis promastigotes forms, as well as their cytotoxicity on mammalian cells and chemical composition.MethodsSixteen EOs were evaluated by mean of IC50/24 h and cytotoxicity against L6 cells (CC50/24 h) using Resazurin assay. Only those EOs that presented better results for IC50/24 h were submitted to GC–MS analysis to determine their chemical constitution.ResultsThe EO from Cinnamodendron dinisii, Matricaria chamomilla, Myroxylon peruiferum, Salvia sclarea, Bulnesia sarmientoi, Ferula galbaniflua, Siparuna guianensis and Melissa officinalis were the most active against L. amazonensis with IC50/24 h ranging from 54.05 to 162.25 μg/mL. Analysis of EOs by GC–MS showed mainly the presence of β-farnesene (52.73 %) and bisabolol oxide (12.09 %) for M. chamomilla; α-copaene (13.41 %), safrole (8.35 %) and δ-cadinene (7.08 %) for M. peruiferum; linalool (28.80 %) and linalyl acetate (60.08 %) for S. sclarea; guaiol (48.29 %) and 2-undecanone (19.49 %) for B. sarmientoi; ethyl phthalate (13.09 %) and methyl-8-pimaren-18-oate (41.82 %) for F. galbaniflua; and neral (37.18 %) and citral (5.02 %) for M. officinalis.ConclusionThe EO from F. galbaniflua showed to be effective against L. amazonensis promastigotes forms and presented low cytotoxic activity against L6 cells. Thus, it represents a strong candidate for future studies aiming its molecular activity on these pathogenic parasites.

In vitro Inhibitory Activity of Essential Oil Vapors against Ascosphaera apis

This work evaluates the in vitro inhibitory activity of 70 essential oils (EOs) in the vapor phase for the control of Chalkbrood disease caused by Ascosphaera apis Maassen ex Claussen (Olive et Spiltoir). Two wild strains isolated from infected honey bee colonies together with one standard collection strain were tested by the microatmosphere method. From 70 EOs, 39 exhibited an antifungal effect against A. apis standard and wild strains. The greatest antifungal action was observed for EO vapors from Armoracia rusticana, followed by Thymus vulgaris, Cymbopogon flexosus, Origanum vulgare and Allium sativum. An investigation of chemical composition by GC-MS revealed, that the most active EOs contained allyl isothiocyanate, citral, carvacrol and diallyl sulfides as the main constituents. The chemical composition plays a key role, as activities of different EOs from the same botanical species were different according to their composition.

Chemical composition and antioxidant activity of essential oils extracted from Egyptian medicinal plants and effect on oxidative stability of soybean oil in storage

The ancient Egyptian culture clearly documented their use of medicinal plants in their hieroglyphic records which are being used as pharmaceutical ingredients, and in food supplementation. Several medicinal plants have been reported to exhibit antioxidant activity. The present study aimed to evaluate the chemical composition of essential oils extracted from Egyptian artemisia, juniper, rosemary, sage and thyme and investigate their antioxidant activities in different test systems as well as in soybean oil. The results showed that rosemary, sage and thyme extracts had the highest amount of polyphenols. Essential oils from medicinal plants were found to contain 25 – 30 components at different concentrations. Camphor, α-thujene and artemisia ketone were the major components of artemisia, α-Pinene and β-phellandrene of the juniper, camphor, α-terpineol and 1,8-cineole of the rosemary, αthujene and camphor of the sage and carvacrol and p-cymene of the thyme. Generally, rosemary, sage and thyme essential oils showed highest antioxidative activities in test systems and were found to be the most effective in retarding the oxidation of soybean oil under both accelerated (60°C for 18 days) and ambient temperatures (22 ± 2°C for 180 days). Antioxidative activities of different essential oils could be related to their polyphenol contents and chemical composition.