Active Lengthening Research Articles

Residual force enhancement decreases when scaling from the single muscle fiber to joint level in humans

BackgroundResidual force enhancement (rFE), defined as increased isometric force following active lengthening compared to a fixed-end isometric contraction at the same muscle length and level of activation, is present across all scales of muscle. While rFE is always present at the cellular level, often rFE “non-responders” are observed during joint-level voluntary contractions. MethodsWe compared rFE between the joint level and single fiber level (vastus lateralis biopsies) in 16 young males. In vivo voluntary knee-extensor rFE was measured by comparing steady-state isometric torque between a stretch-hold (maximal activation at 150°, stretch to 70°, hold) and a fixed-end isometric contraction, with ultrasonographic recording of vastus lateralis fascicle length (FL). Fixed-end contractions were performed at 67.5°, 70.0°, 72.5°, and 75.0°; the joint angle that most closely matched FL of the stretch-hold contraction's isometric steady-state was used to calculate rFE. The starting and ending FLs of the stretch-hold contraction were expressed as % optimal FL, determined via torque-angle relationship. ResultsIn single fiber experiments, the starting and ending fiber lengths were matched relative to optimal length determined from in vivo testing, yielding an average sarcomere excursion of ∼2.2–3.4µm. There was a greater magnitude of rFE at the single fiber (∼20%) than joint level (∼5%) (p = 0.004), with “non-responders” only observed at the joint level. ConclusionBy comparing rFE across scales within the same participants, we show the development of the rFE non-responder phenomenon is upstream of rFE's cellular mechanisms, with rFE only lost rather than gained when scaling from single fibers to the joint level.

A benchmark of muscle models to length changes great and small

Digital human body models are used to simulate injuries that occur as a result of vehicle collisions, vibration, sports, and falls. Given enough time the body’s musculature can generate force, affect the body’s movements, and change the risk of some injuries. The finite-element code LS-DYNA is often used to simulate the movements and injuries sustained by the digital human body models as a result of an accident. In this work, we evaluate the accuracy of the three muscle models in LS-DYNA (MAT_156, EHTM, and the VEXAT) when simulating a range of experiments performed on isolated muscle: force–length–velocity experiments on maximally and sub-maximally stimulated muscle, active-lengthening experiments, and vibration experiments. The force–length–velocity experiments are included because these conditions are typical of the muscle activity that precedes an accident, while the active-lengthening and vibration experiments mimic conditions that can cause injury. The three models perform similarly during the maximally and sub-maximally activated force–length–velocity experiments, but noticeably differ in response to the active-lengthening and vibration experiments. The VEXAT model is able to generate the enhanced forces of biological muscle during active lengthening, while both the MAT_156 and EHTM produce too little force. In response to vibration, the stiffness and damping of the VEXAT model closely follows the experimental data while the MAT_156 and EHTM models differ substantially. The accuracy of the VEXAT model comes from two additional mechanical structures that are missing in the MAT_156 and EHTM models: viscoelastic cross-bridges, and an active titin filament. To help others build on our work we have made our simulation code publicly available.

Phenomenological Muscle Constitutive Model With Actin-Titin Binding for Simulating Active Stretching.

The force produced by a muscle depends on its contractile history, yet human movement simulations typically employ muscle models that define the force-length relationship from measurements of fiber force during isometric contractions. In these muscle models, the total force-length curve can have a negative slope at fiber lengths greater than the fiber length at which peak isometric force is produced. This region of negative stiffness can cause numerical instability in simulations. Experiments have found that the steady-state force in a muscle fiber following active stretching is greater than the force produced during a purely isometric contraction. This behavior is called residual force enhancement. We present a constitutive model that exhibits force enhancement, implemented as a hyperelastic material in the febio finite element software. There is no consensus on the mechanisms responsible for force enhancement; we adopt the assumption that the passive fiber force depends on the sarcomere length at the instant that the muscle is activated above a threshold. We demonstrate the numerical stability of our model using an eigenvalue analysis and by simulating a muscle whose fibers are of different lengths. We then use a three-dimensional muscle geometry to verify the effect of force enhancement on the development of stress and the distribution of fiber lengths. Our proposed muscle material model is one of the few models available that exhibits force enhancement and is suitable for simulations of active lengthening. We provide our implementation in febio so that others can reproduce and extend our results.

A three filament mechanistic model of musculotendon force and impedance

The force developed by actively lengthened muscle depends on different structures across different scales of lengthening. For small perturbations, the active response of muscle is well captured by a linear-time-invariant (LTI) system: a stiff spring in parallel with a light damper. The force response of muscle to longer stretches is better represented by a compliant spring that can fix its end when activated. Experimental work has shown that the stiffness and damping (impedance) of muscle in response to small perturbations is of fundamental importance to motor learning and mechanical stability, while the huge forces developed during long active stretches are critical for simulating and predicting injury. Outside of motor learning and injury, muscle is actively lengthened as a part of nearly all terrestrial locomotion. Despite the functional importance of impedance and active lengthening, no single muscle model has all these mechanical properties. In this work, we present the viscoelastic-crossbridge active-titin (VEXAT) model that can replicate the response of muscle to length changes great and small. To evaluate the VEXAT model, we compare its response to biological muscle by simulating experiments that measure the impedance of muscle, and the forces developed during long active stretches. In addition, we have also compared the responses of the VEXAT model to a popular Hill-type muscle model. The VEXAT model more accurately captures the impedance of biological muscle and its responses to long active stretches than a Hill-type model and can still reproduce the force-velocity and force-length relations of muscle. While the comparison between the VEXAT model and biological muscle is favorable, there are some phenomena that can be improved: the low frequency phase response of the model, and a mechanism to support passive force enhancement.

A three filament mechanistic model of musculotendon force and impedance.

The force developed by actively lengthened muscle depends on different structures across different scales of lengthening. For small perturbations, the active response of muscle is well captured by a linear-time-invariant (LTI) system: a stiff spring in parallel with a light damper. The force response of muscle to longer stretches is better represented by a compliant spring that can fix its end when activated. Experimental work has shown that the stiffness and damping (impedance) of muscle in response to small perturbations is of fundamental importance to motor learning and mechanical stability, while the huge forces developed during long active stretches are critical for simulating and predicting injury. Outside of motor learning and injury, muscle is actively lengthened as a part of nearly all terrestrial locomotion. Despite the functional importance of impedance and active lengthening, no single muscle model has all these mechanical properties. In this work, we present the viscoelastic-crossbridge active-titin (VEXAT) model that can replicate the response of muscle to length changes great and small. To evaluate the VEXAT model, we compare its response to biological muscle by simulating experiments that measure the impedance of muscle, and the forces developed during long active stretches. In addition, we have also compared the responses of the VEXAT model to a popular Hill-type muscle model. The VEXAT model more accurately captures the impedance of biological muscle and its responses to long active stretches than a Hill-type model and can still reproduce the force-velocity and force-length relations of muscle. While the comparison between the VEXAT model and biological muscle is favorable, there are some phenomena that can be improved: the low frequency phase response of the model, and a mechanism to support passive force enhancement.

Stretch-shortening cycles protect against the age-related loss of power generation in rat single muscle fibres

Aging is associated with impaired strength and power during isometric and shortening contractions, however, during lengthening (i.e., eccentric) contractions, strength is maintained. During daily movements, muscles undergo stretch-shortening cycles (SSCs). It is unclear whether the age-related maintenance of eccentric strength offsets age-related impairments in power generation during SSCs owing to the utilization of elastic energy or other cross-bridge based mechanisms. Here we investigated how aging influences SSC performance at the single muscle fibre level and whether performing active lengthening prior to shortening protects against age-related impairments in power generation. Single muscle fibres from the psoas major of young (∼8 months; n = 31 fibres) and old (∼32 months; n = 41 fibres) male F344BN rats were dissected and chemically permeabilized. Fibres were mounted between a force transducer and length controller and maximally activated (pCa 4.5). For SSCs, fibres were lengthened from average sarcomere lengths of 2.5 to 3.0 μm and immediately shortened back to 2.5 μm at both fast and slow (0.15 and 0.60 Lo/s) lengthening and shortening speeds. The magnitude of the SSC effect was calculated by comparing work and power during shortening to an active shortening contraction not preceded by active lengthening. Absolute isometric force was ∼37 % lower in old compared to young rat single muscle fibres, however, when normalized to cross-sectional area (CSA), there was no longer a significant difference in isometric force between age groups, meanwhile there was an ∼50 % reduction in absolute power in old as compared with young. We demonstrated that SSCs significantly increased power production (75–110 %) in both young and old fibres when shortening occurred at a fast speed and provided protection against power-loss with aging. Therefore, in older adults during everyday movements, power is likely ‘protected’ in part due to the stretch-shortening cycle as compared with isolated shortening contractions.

Biceps femoris long head muscle and aponeurosis geometry in males with and without a history of hamstring strain injury.

Hamstring strain injuries (HSIs) commonly affect the proximal biceps femoris long head (BFlh) musculotendinous junction. Biomechanical modeling suggests narrow proximal BFlh aponeuroses and large muscle-to-aponeurosis width ratios increase localized tissue strains and presumably risk of HSI. This study aimed to determine if BFlh muscle and proximal aponeurosis geometry differed between limbs with and without a history of HSI. Twenty-six recreationally active males with (n = 13) and without (n = 13) a history of unilateral HSI in the last 24 months underwent magnetic resonance imaging of both thighs. BFlh muscle and proximal aponeurosis cross-sectional areas, length, volume, and interface area between muscle and aponeurosis were extracted. Previously injured limbs were compared to uninjured contralateral and control limbs for discrete variables and ratios, and along the relative length of tissues using statistical parametric mapping. Previously injured limbs displayed significantly smaller muscle-to-aponeurosis volume ratios (p = 0.029, Wilcoxon effect size (ES) = 0.43) and larger proximal BFlh aponeurosis volumes (p = 0.019, ES = 0.46) than control limbs with no history of HSI. No significant differences were found between previously injured and uninjured contralateral limbs for any outcome measure (p = 0.216-1.000, ES = 0.01-0.36). Aponeurosis geometry differed between limbs with and without a history of HSI. The significantly larger BFlh proximal aponeuroses and smaller muscle-to-aponeurosis volume ratios in previously injured limbs could alter the strain experienced in muscle adjacent to the musculotendinous junction during active lengthening. Future research is required to determine if geometric differences influence the risk of re-injury and whether they can be altered via targeted training.

Conserved mammalian muscle mechanics during eccentric contractions.

Skeletal muscle has a broad range of biomechanical functions, including power generation and energy absorption. These roles are underpinned by the force-velocity relationship, which comprises two distinct components: a concentric and an eccentric force-velocity relationship. The concentric component has been extensively studied across a wide range of muscles with different muscle properties. However, to date, little progress has been made in accurately characterising the eccentric force-velocity relationship in mammalian muscle with varying muscle properties. Consequently, mathematical models of this muscle behaviour are based on a poorly understood phenomenon. Here, we present a comprehensive assessment of the concentric force-velocity and eccentric force-velocity relationships of four mammalian muscles (soleus, extensor digitorum longus, diaphragm and digastric) with varying biomechanical functions, spanning three orders of magnitude in body mass (mouse, rat and rabbits). The force-velocity relationship was characterised using a hyperbolic-linear equation for the concentric component a hyperbolic equation for the eccentric component, at the same time as measuring the rate of force development in the two phases of force development in relation to eccentric lengthening velocity. We demonstrate that, despite differences in the curvature and plateau height of the eccentric force-velocity relationship, the rates of relative force development were consistent for the two phases of the force-time response during isovelocity lengthening ramps, in relation to lengthening velocity, in the four muscles studied. Our data support the hypothesis that this relationship depends on cross-bridge and titin activation. Hill-type musculoskeletal models of the eccentric force-velocity relationship for mammalian muscles should incorporate this biphasic force response. KEY POINTS: The capacity of skeletal muscle to generate mechanical work and absorb energy is underpinned by the force-velocity relationship. Despite identification of the lengthening (eccentric) force-velocity relationship over 80years ago, no comprehensive study has been undertaken to characterise this relationship in skeletal muscle. We show that the biphasic force response seen during active muscle lengthening is conserved over three orders of magnitude of mammalian skeletal muscle mass. Using mice with a small deletion in titin, we show that part of this biphasic force profile in response to muscle lengthening is reliant on normal titin activation. The rate of force development during muscle stretch may be a more reliable way to describe the forces experienced during eccentric muscle contractions compared to the traditional hyperbolic curve fitting, and functions as a novel predictor of force-velocity characteristics that may be used to better inform hill-type musculoskeletal models and assess pathophysiological remodelling.

Human in vivo medial gastrocnemius gear during active and passive muscle lengthening: effect of inconsistent methods and nomenclature on data interpretation.

'Muscle gear' is calculated as the ratio of fascicle-to-muscle length change, strain, or velocity. Inconsistencies in nomenclature and definitions of gear exist across disciplines partly due to differences in fascicle [curved (Lf) versus linear (Lf,straight)] and muscle [whole-muscle belly (Lb) versus belly segment (Lb,segment)] length calculation methods. We tested whether these differences affect gear magnitude during passive and active muscle lengthening of human medial gastrocnemius of young men (n=13, 26.3±5.0 years) using an isokinetic dynamometer. Lb, Lb,segment, Lf and Lf,straight were measured from motion analysis and ultrasound imaging data. Downshifts in belly gear but not belly segment gear occurred with muscle lengthening only during active lengthening. Muscle gear was unaffected by fascicle length measurement method (P=0.18) but differed when calculated as changes in Lb or Lb,segment (P<0.01) in a length-dependent manner. Caution is therefore advised for the use and interpretation of different muscle gear calculation methods and nomenclatures in animal and human comparative physiology.

A Conceptual Exploration of Hamstring Muscle-Tendon Functioning during the Late-Swing Phase of Sprinting: The Importance of Evidence-Based Hamstring Training Frameworks.

An eccentrically lengthening, energy-absorbing, brake-driven model of hamstring function during the late-swing phase of sprinting has been widely touted within the existing literature. In contrast, an isometrically contracting, spring-driven model of hamstring function has recently been proposed. This theory has gained substantial traction within the applied sporting world, influencing understandings of hamstring function while sprinting, as well as the development and adoption of certain types of hamstring-specific exercises. Across the animal kingdom, both spring- and motor-driven muscle-tendon unit (MTU) functioning are frequently observed, with both models of locomotive functioning commonly utilising some degree of active muscle lengthening to draw upon force enhancement mechanisms. However, a method to accurately assess hamstring muscle-tendon functioning when sprinting does not exist. Accordingly, the aims of this review article are three-fold: (1) to comprehensively explore current terminology, theories and models surrounding muscle-tendon functioning during locomotion, (2) to relate these models to potential hamstring function when sprinting by examining a variety of hamstring-specific research and (3) to highlight the importance of developing and utilising evidence-based frameworks to guide hamstring training in athletes required to sprint. Due to the intensity of movement, large musculotendinous stretches and high mechanical loads experienced in the hamstrings when sprinting, it is anticipated that the hamstring MTUs adopt a model of functioning that has some reliance upon active muscle lengthening and muscle actuators during this particular task. However, each individual hamstring MTU is expected to adopt various combinations of spring-, brake- and motor-driven functioning when sprinting, in accordance with their architectural arrangement and activation patterns. Muscle function is intricate and dependent upon complex interactions between musculoskeletal kinematics and kinetics, muscle activation patterns and the neuromechanical regulation of tensions and stiffness, and loads applied by the environment, among other important variables. Accordingly, hamstring function when sprinting is anticipated to be unique to this particular activity. It is therefore proposed that the adoption of hamstring-specific exercises should not be founded on unvalidated claims of replicating hamstring function when sprinting, as has been suggested in the literature. Adaptive benefits may potentially be derived from a range of hamstring-specific exercises that vary in the stimuli they provide. Therefore, a more rigorous approach is to select hamstring-specific exercises based on thoroughly constructed evidence-based frameworks surrounding the specific stimulus provided by the exercise, the accompanying adaptations elicited by the exercise, and the effects of these adaptations on hamstring functioning and injury risk mitigation when sprinting.

Diagnostic potential of myocardial early systolic lengthening for patients with suspected non-ST-segment elevation acute coronary syndrome

BackgroundDuring early systole, ischemic myocardium with reduced active force experiences early systolic lengthening (ESL). This study aimed to explore the diagnostic potential of myocardial ESL in suspected non-ST-segment elevation acute coronary syndrome (NSTE-ACS) patients with normal wall motion and left ventricular ejection fraction (LVEF).MethodsOverall, 195 suspected NSTE-ACS patients with normal wall motion and LVEF, who underwent speckle tracking echocardiography (STE) before coronary angiography, were included in this study. Patients were stratified into the coronary artery disease (CAD) group when there was ≥ 50% stenosis in at least one major coronary artery. The CAD patients were further stratified into the significant (≥ 70% reduction of vessel diameter) stenosis group or the nonsignificant stenosis group. Myocardial strain parameters, including global longitudinal strain (GLS), duration of early systolic lengthening (DESL), early systolic index (ESI), and post-systolic index (PSI), were analyzed using STE and compared between groups. Receiver operating characteristic curve (ROC) analysis was performed to determine the diagnostic accuracy. Logistic regression analysis was conducted to establish the independent and incremental determinants for the presence of significant coronary stenosis.ResultsThe DESL and ESI values were higher in patients with CAD than those without CAD. In addition, CAD patients with significant coronary stenosis had higher DESL and ESI than those without significant coronary stenosis. The ROC analysis revealed that ESI was superior to PSI for identifying patients with CAD, and further superior to GLS and PSI for predicting significant coronary stenosis. Moreover, ESI could independently and incrementally predict significant coronary stenosis in patients with CAD.ConclusionsThe myocardial ESI is of great value for the diagnosis and risk stratification of clinically suspected NSTE-ACS patients with normal LVEF and wall motion.

Connective Adaptive Resistance Exercise (CARE) Machines for Accentuated Eccentric and Eccentric-Only Exercise: Introduction to an Emerging Concept

Eccentric resistance exercise emphasizes active muscle lengthening against resistance. In the past 15 years, researchers and practitioners have expressed considerable interest in accentuated eccentric (i.e., eccentric overload) and eccentric-only resistance exercise as strategies for enhancing performance and preventing and rehabilitating injuries. However, delivery of eccentric resistance exercise has been challenging because of equipment limitations. Previously, we briefly introduced the concept of connected adaptive resistance exercise (CARE)—the integration of software and hardware to provide a resistance that adjusts in real time and in response to the individual’s volitional force within and between repetitions. The aim of the current paper is to expand this discussion and explain the potential for CARE technology to improve the delivery of eccentric resistance exercise in various settings. First, we overview existing resistance exercise equipment and highlight its limitations for delivering eccentric resistance exercise. Second, we describe CARE and explain how it can accomplish accentuated eccentric and eccentric-only resistance exercise in a new way. We supplement this discussion with preliminary data collected with CARE technology in laboratory and non-laboratory environments. Finally, we discuss the potential for CARE technology to deliver eccentric resistance exercise for various purposes, e.g., research studies, rehabilitation programs, and home-based or telehealth interventions. Overall, CARE technology appears to permit completion of eccentric resistance exercise feasibly in both laboratory and non-laboratory environments and thus has implications for researchers and practitioners in the fields of sports medicine, physiotherapy, exercise physiology, and strength and conditioning. Nevertheless, formal investigations into the impact of CARE technology on participation in eccentric resistance exercise and clinical outcomes are still required.

Regulation of primary afferent depolarization and homosynaptic post-activation depression during passive and active lengthening, shortening and isometric conditions.

This study aimed to determine whether the modulation of primary afferent depolarization (PAD) and homosynaptic post-activation depression (HPAD) are involved in the lower efficacy of Ia-afferent-α-motoneuron transmission commonly observed during lengthening compared to isometric and shortening conditions. 15 healthy young individuals participated in two experimental sessions dedicated to measurement in passive and active muscle states, respectively. In each session, PAD, HPAD and the efficacy of Ia-afferent-α-motoneuron transmission were evaluated during lengthening, shortening and isometric conditions. PAD was evaluated with D1 inhibition technique. Posterior tibial nerve stimulation was used to study HPAD and the efficacy of the Ia-afferent-α-motoneuron transmission through the recording of the soleus Hoffmann reflex (H reflex). PAD was increased in lengthening than shortening (11.2%) and isometric (12.3%) conditions regardless of muscle state (P < 0.001). HPAD was increased in lengthening than shortening (5.1%) and isometric (4.2%) conditions in the passive muscle state (P < 0.05), while no difference was observed in the active muscle state. H reflex was lower in lengthening than shortening (-13.2%) and isometric (-9.4%) conditions in both muscle states (P < 0.001). These results highlight the specific regulation of PAD and HPAD during lengthening conditions. However, the differences observed during passive lengthening compared to shortening and isometric conditions seem to result from an increase in Ia-afferent discharge, while the variations highlighted during active lengthening might come from polysynaptic descending pathways involving supraspinal centres that could regulate PAD mechanism.

The day-to-day reliability of residual force enhancement during voluntary and electrically stimulated contractions.

Residual force enhancement (rFE) is characterized by increased steady-state isometric force following active muscle lengthening compared to a fixed-end isometric contraction at the same muscle length and level of neuromuscular activation. Many studies have characterized rFE in humans; however, the day-to-day reliability of rFE is unclear. We aimed to examine day-to-day reliability of rFE across various contraction types in the dorsiflexors in males and females. Twenty-five recreationally active young adults completed 2 visits, 1 week apart. Following determination of maximum voluntary contraction (MVC) strength, rFE was assessed during maximal voluntary effort, 20% MVC electrically stimulated, and 20% MVC torque-matching conditions. Each rFE condition was completed at 2 joint excursions: 0-20º plantar flexion (PF) and 0-40ºPF. Intraclass correlation coefficients (ICC) assessed relative reliability, and typical error of measurement (TEM), and the correlation variability of TEM (CVTEM) assessed absolute reliability. Electrically stimulated contractions demonstrated the highest reliability at 40ºPF (ICC:0.9; CVTEM:22.8 %) and 20ºPF (ICC:0.8; CVTEM:34.3 %), followed by maximal voluntary contractions at 40ºPF (ICC:0.7; CVTEM:55.1%) and 20ºPF (ICC:0.1; CVTEM:81.1%). The torque-matching trials showed poor reliability for 20º and 40ºPF (ICC: -0.1-0.3; CVTEM: 118.1 %-155.2 %). Our results demonstrate higher reliability of rFE when stretching to the descending limb of the torque-angle relationship compared to the plateau region, and in electrically stimulated compared to voluntary contractions in the dorsiflexors for both males and females.

Divergent runoff impacts of permafrost and seasonally frozen ground at a large river basin of Tibetan Plateau during 1960–2019

Since the 20th century, due to global warming, permafrost areas have undergone significant changes. The degradation of permafrost has complicated water cycle processes. Taking the upper Yellow River basin (UYRB) as a demonstration, this study discusses the long-term (1960–2019) changes in frozen ground and their hydrological effects with a cryosphere-hydrology model, in particular a permafrost version of the water and energy budget-based distributed hydrological model. The permafrost at the UYRB, with thickening active layer and lengthening thawing duration, has degraded by 10.8%. The seasonally frozen ground has a more pronounced intra-annual regulation that replenishes surface runoff in the warm season, while the degradation of permafrost leads to a runoff increase. The occurrence of extreme events at the UYRB has gradually decreased with the degradation of frozen ground, but spring droughts and autumn floods become more serious. The results may help better understand the hydrological impacts of permafrost degradation in the Tibetan Plateau.

Eccentric exercise-induced muscle weakness amplifies the history dependence of force.

Following active lengthening or shortening contractions, isometric steady-state torque is increased (residual force enhancement; rFE) or decreased (residual force depression; rFD), respectively, compared to fixed-end isometric contractions at the same muscle length and level of activation. Though the mechanisms underlying this history dependence of force have been investigated extensively, little is known about the influence of exercise-induced muscle weakness on rFE and rFD. Assess rFE and rFD in the dorsiflexors at 20%, 60%, and 100% maximal voluntary torque (MVC) and activation matching, and electrically stimulated at 20% MVC, prior to, 1h following, and 24h following 150 maximal eccentric dorsiflexion contractions. Twenty-six participants (13 male, 24.7 ± 2.0y; 13 female, 22.5 ± 3.6y) were seated in a dynamometer with their right hip and knee angle set to 110° and 140°, respectively, with an ankle excursion set between 0° and 40° plantar flexion (PF). MVC torque, peak twitch torque, and prolonged low frequency force depression were used to assess eccentric exercise-induced neuromuscular impairments. History-dependent contractions consisted of a 1s isometric (40°PF or 0°PF) phase, a 1s shortening or lengthening phase (40°/s), and an 8s isometric (0°PF or 40°PF) phase. Following eccentric exercise; MVC torque was decreased, prolonged low frequency force depression was present, and both rFE and rFD increased for all maximal and submaximal conditions. The history dependence of force during voluntary torque and activation matching, and electrically stimulated contractions is amplified following eccentric exercise. It appears that a weakened neuromuscular system amplifies the magnitude of the history-dependence of force.

Influence of weighted downhill running training on serial sarcomere number and work loop performance in the rat soleus.

ABSTRACTIncreased serial sarcomere number (SSN) has been observed in rats following downhill running training due to the emphasis on active lengthening contractions; however, little is known about the influence on dynamic contractile function. Therefore, we employed 4 weeks of weighted downhill running training in rats, then assessed soleus SSN and work loop performance. We hypothesised trained rats would produce greater net work output during work loops due to a greater SSN. Thirty-one Sprague-Dawley rats were assigned to a training or sedentary control group. Weight was added during downhill running via a custom-made vest, progressing from 5–15% body mass. Following sacrifice, the soleus was dissected, and a force-length relationship was constructed. Work loops (cyclic muscle length changes) were then performed about optimal muscle length (LO) at 1.5–3-Hz cycle frequencies and 1–7-mm length changes. Muscles were then fixed in formalin at LO. Fascicle lengths and sarcomere lengths were measured to calculate SSN. Intramuscular collagen content and crosslinking were quantified via a hydroxyproline content and pepsin-solubility assay. Trained rats had longer fascicle lengths (+13%), greater SSN (+8%), and a less steep passive force-length curve than controls (P<0.05). There were no differences in collagen parameters (P>0.05). Net work output was greater (+78–209%) in trained than control rats for the 1.5-Hz work loops at 1 and 3-mm length changes (P<0.05), however, net work output was more related to maximum specific force (R2=0.17-0.48, P<0.05) than SSN (R2=0.03-0.07, P=0.17-0.86). Therefore, contrary to our hypothesis, training-induced sarcomerogenesis likely contributed little to the improvements in work loop performance.This article has an associated First Person interview with the first author of the paper.

INFLUENCE OF ADDITIONAL INHIBITORS ON CATALYTIC DESTRUCTION REACTION OF POLYISOBUTYLENE

It is impossible to create a scientific basis for the synthesis of polymers without taking into account the destruction process. Under the action of Lewis acids, the destruction of PIB follows a free radical chain mechanism, while the catalytic destruction of PIB leads to the formation of both linear and branched macromolecules. The most wide-spread type of chemical destruction is an oxidative destruction by oxygen and ozone. The oxidative destruction is activated by heat, light rays and mechanical energy, and the types of thermal, photo and mechanical oxidative destruction are known, respectively. As a result of these destructive reactions, both the composition and the structure of polymer change, that is, it “grows old”. The aging of a polymer means a change in its physical-chemical, physical-mechanical properties during exploitation . The fastest oxidative destruction of polymers occurs by a chain mechanism (with free radicals), as in low molecular weight hydrocarbons. The oxidation process of polymers without double bonds in the chains proceeds weakly, and the formation of peroxide groups is not always observed in them. This process consists of three stages: formation of an active center, lengthening and breaking of the chain. It is known that oxidation begins with the formation of peroxides. Active centers are obtained by the decomposition of peroxides. When studying the chemistry of the reaction, it was noticed that there is another mechanism for the destruction of polyisobutylene in the presence of strong Lewis acids, characteristic of cationic processes. One of the interesting aspects of the destruction process is a complete understanding of the "chemistry" of catalytic destruction. For this, various additives must be added to the catalytic reaction. During the experiments it has been found that the reaction does not take place in a completely dried solvent. The reaction proceeds when a significant amount of water is added to the system. The amount of water in the system is [H2O] ≈10-3 mol / l. This can be explained by the fact that the catalyst in dimer form is inactive in the nonpolar helix, but the water plays the role of a polar additive, decomposing the dimer catalyst and converting it into the monomeric form of Brønsted acid. Therefore, the inhibitory effect of 2,6-DTBP and toluene on the reaction has been studied in order to fully elucidate the chemistry of the catalytic destruction of PIB by Lewis acids. Since 2,6-DTBP is a trap for protons, it plays an important role in the detection of the active form of the catalyst. The ineffectiveness of 2,6-DTBP and toluene as an inhibitor led to the existence of a radical reaction mechanism and the discovery of the active form of the catalyst. For better understanding the reaction mechanism, the inhibitory effect of O2, p-benzoquinone and tetracyanethylene on the reverse process has been studied. The formation of block copolymers upon modification of PIB with acrylonitrile and vinyl acetate, polymerized by the radical mechanism, not only confirms the radical mechanism of destruction, but also creates conditions for the production of new copolymers. The formation of active centers has been also confirmed by the EPR method, free macroradicals have been detected and their density has been determined. The formation of free macroradicals as a result of the direct action of strong mechanical energy, direct mechanical cracking or transfer of vibration energy of the initial highly active state of the polymer chain is the initial stage of mechanical and chemical processes. The overall final result of the transformations depends on the direction of the subsequent secondary reactions of free macroradicals. Although these reactions are different, they correspond to typical free radical processes in polymer systems. On the whole, destructive reactions are considered one of the most important reactions for high molecular weight compounds. They are used to study the structure of high molecular weight compounds, as well as to obtain low molecular weight compounds from the natural polymers. All this has been reflected in IR, UV, NMR, EPR spectroscopic studies. A mathematical and kinetic model of the processes has been developed and parameters have been calculated using the experimental indicators of the carried out researches. Keywords: catalyst, 2,6-DTBP, toluene, polyisobutylene, destruction, halide, aikyl halide, inhibitor, deactivation, methano

A 3D model of the soleus reveals effects of aponeuroses morphology and material properties on complex muscle fascicle behavior

The soleus is an important plantarflexor muscle with complex fascicle and connective tissue arrangement. In this study we created an image-based finite element model representing the 3D structure of the soleus muscle and its aponeurosis connective tissue, including distinct fascicle architecture of the posterior and anterior compartments. The model was used to simulate passive and active soleus lengthening during ankle motion to predict tissue displacements and fascicle architecture changes. Both the model’s initial architecture and changes incurred during passive lengthening were consistent with prior in vivo data from diffusion tensor imaging. Model predictions of active lengthening were consistent with axial plane muscle displacements that we measured in eight subjects’ lower legs using cine DENSE (Displacement Encoding with Stimulated Echoes) MRI during eccentric dorsiflexion. Regional strains were variable and nonuniform in the model, but average fascicle strains were similar between the compartments for both passive (anterior: 0.18 ± 0.06, posterior: 0.19 ± 0.05) and active (anterior: 0.12 ± 0.05, posterior: 0.13 ± 0.06) lengthening and were two- to three-times greater than muscle belly strain (0.06). We used additional model simulations to investigate the effects of aponeurosis material properties on muscle deformation, by independently varying the longitudinal or transverse stiffness of the posterior or anterior aponeurosis. Results of model variations elucidate how properties of soleus aponeuroses contribute to fascicle architecture changes. Greater longitudinal stiffness of posterior compared to anterior aponeurosis promoted more uniform spatial distribution of muscle tissue deformation. Reduced transverse stiffness in both aponeuroses resulted in larger differences between passive and active soleus lengthening.

Spinopelvic adaptations between standing and sitting positions in patients with adult spinal deformity

This article introduces history-dependent effects in a skeletal muscle model applied to dynamic simulations of musculoskeletal system motion. Force depression and force enhancement induced by active muscle shortening and lengthening, respectively, represent muscle history effects. A muscle model depending on the preceding contractile events together with the current parameters was developed for OpenSim software, and applied in simulations of standing heel-raise and squat movements. Muscle activations were computed using joint kinematics and ground reaction forces recorded from the motion capture of seven individuals. In the muscle-actuated simulations, a modification was applied to the computed activation, and was compared to the measured electromyography data. For the studied movements, the history gives a small but visible effect to the muscular force trace, but some parameter values must be identified before the exact magnitude can be analysed. The muscle model modification improves the existing muscle models and gives a more accurate description of underlying forces and activations in musculoskeletal system movement simulations.