Activated Charcoal Column Research Articles

Column chromatographic separation of radioactive tellurate, tellurite, iodide and iodate by active charcoal

Abstract The adsorption behaviour of trace amounts of tellurate, tellurite, iodide and iodate on active charcoal has been studied by a radioactive-tracer method; on the basis of the results, a column chromatographic separation of these ions from each other was devised. By passing the sample solution (at pH 5.6) through an active charcoal column, all but tellurate are retained, lodate, tellurite and iodide are then eluted successively with water (pH 7.0), 0.1 N hydrochloric acid and 1 N sodium hydroxide, respectively. The method can be applied to the preparation of radioiodine from a neutron-irradiated tellurate target.

Studies on the metabolism of chlorophenols in fish. V. Isolation and identification of a conjugated phenol excreted by goldfish.

A conjugated phenol excreted by goldfish, Carassius auratus, into the culture medium was isolated by treating the medium with activated charcoal columns, followed by elution with acetone-water and/or ammonia mixtures, and finally passing the concentrated eluate, under reduced pressure, through a Sephadex G-10 column. The isolated conjugate was identified as phenylsulfate by elution patterns on columns of charcoal and Sephadex, pre-cipitation with BaCl2, hydrolysis on heating in acidic solution to release free-phenol, thin layer chromatography, UV-absorption spectra and the molar ratio of phenol to SO4, com-paring it with the synthesized phenylsulfate. Glucuronide was not detected, although ADAMSON et al. (1967, 1972, 1974) have reported the formation of glucuronides for some phenolic compounds in fishes. The present study shows that sulfate conjugation is one of the most general detoxication mechanisms for phenolic compounds in fish, at least in goldfish, contrary to the conclusion of MAICKEL et al. (1958) that goldfish were completely incapable of forming either glucuronides or sulfates for phenols.

Determination of Dimethylpolysiloxane in Foods by Atomic Absorption Spectrophotometry

The determination of dimethylpolysiloxane (DMPS) in foods by atomic absorption spectrophotometry was studied. The separation of DMPS from the edible oil was carried out as follows: The sample mixed with DEAE cellulose powder placed on a DEAE cellulose column kept at -70°C, then n-hexane was poured into the column. The solvent of the eluate was removed, and the residual was dissolved in ethyl ether. Other samples were extracted with ethyl ether. The ethyl ether solution was cleaned up with activated charcoal column. Ether in the eluates were removed, and the residual was redissolved in methyl isobutyl ketone. The operating conditions were as follows:Analytical line; 2516A for SiLamp current; 40mANitrous oxide flow rate; 13.0L/minAcetylene flow rate; 8.5L/minThe recoveries of DMPS added in food were more than 95%. The presence of silicon dioxide and silicate in foods at natural level did not interfere. As the results of the survey of commercial foods by the use of above mentioned procedures, DMPS was detected in some samples.