Activation Of Reparative Processes Research Articles

Prevention of calcium and vitamin D deficiency in osteoporosis: possibility of using combined drugs Osteomed, Osteomed Forte, Osteo-Vit D3

Objective. To analyse literature data reflecting the issue of prevention of calcium and vitamin D deficiency in osteoporosis. Material and methods. Analysis of 47 Russian and foreign publications on prevention of calcium and vitamin D deficiency in osteoporosis was carried out. Results and discussion. The role of vitamin D in maintenance of calcium homeostasis and bone metabolism has been shown. Lack of vitamin D is associated with the development of osteomalacia and osteoporosis in adults and rickets in children. It was noted that hypovitaminosis D was recognised as a worldwide pandemic. It was acknowledged that there was a need to develop dietary recommendations, supplementary intake of vitamin D to prevent, among other things, bone diseases in most countries of the world. The results of the study of the Russian drugs Osteomed, Osteomed Forte, Osteo-Vit D3 (Parapharm, Russia) were presented. Conclusions. Thus, combined preparations of Ca and vitamin D were of great importance for prevention and complex treatment of osteoporosis, for the compensation of calcium deficiency in adolescence, elderly, during pregnancy, lactation and after menopause. Combined calcium and vitamin D supplementation was more effective than monotherapy in slowing bone loss, reducing the risk of fractures, strengthening the skeletal musculature and reducing the probability of falls, disability and death, but promotes calcification of soft tissues and blood vessels. At the same time, the only osteoprotectors without the risk of calcification of blood vessels and soft tissue, as well as the development of atherosclerosis, to date recognized by the Russian line of drugs "Osteomed. The safety of this group of dietary supplements, including their use in the treatment of bone pathologies in children and adolescents, is ensured by the presence of a unique HDBA organic complex. This component, by correcting the hormonal status and maintaining an optimal level of androgens within the age norm, has an anabolic effect, stimulating the birth of new bone cells. It is activation of reparative processes at the cellular level that enables to realize the effect of targeted delivery of calcium into bone tissue.

IMPROVING THE EFFICIENCY OF TREATMENT OF TROPHIC ULCERS IN THE LOWER EXTREMITIES RESULTED FROM CHRONIC VENOUS INSUFFICIENCY

The prevalence of varicose veins of the lower extremities and trophic ulcers, and as a consequence, deterioration of the quality of life, high rate of disability pose this problem as one of the social and healthcare challenges. The aim of the study is to evaluate the effectiveness of the low-intensity laser radiation in the integrated treatment of trophic ulcers of the lower extremities. Clinical and histomorphological studies were performed on 75 patients, 21 men and 54 women, aged from 15 to 75 years, who took the course of treatment for small and medium stage II ulcers on the lower extremities at the Educational and Surgical Clinic of the Azerbaijan Medical University and the Central Hospital of the Gazakh district. Criteria for inclusion in the study: both sexes, the presence of ulcerative necrotic lesions of the lower extremities. Exclusion criteria: heart failure, systemic diseases, cancerous diseases, hormone therapy, pregnancy. To conduct a comparative analysis, patients were randomly divided into three groups (25 individuals in each group): the main group, the comparison group and the control group. Venocoryl ointment, low-intensity laser radiation and endovenous laser ablation were used in the treatment of trophic ulcers of the lower extremities of small and medium sizes in the main group. The control group received regional treatment including standard retro-specific methods and endovenous laser ablation. The comparison group received endovenous laser ablation and Venocoryl ointment. When using Venocoryl ointment, low-intensity laser radiation and endovenous laser ablation in the treatment of trophic ulcers with leg varicose veins, a more pronounced decrease in pain sensations, faster relief of the inflammatory process and activation of reparative processes were observed on the 7th and 14th days after the beginning of the therapy compared to the control group and the comparison group. The use of Venocoryl ointment, low-intensity laser radiation and endovenous laser ablation reduces pain in the main group after 7 and 14 days compared with the control group.

Modern aspects of the treatment of post-traumatic deforming arthrosis of the ankle joint

Post-traumatic deforming arthrosis of the ankle joint is a severe complication of damage to articular structures. The severity of its clinical manifestations depends on a number of factors: the time elapsed since the injury, the severity of primary anatomical disorders, the activity of reparative processes, the presence of tibiofibular diastasis, the state of the ligamentous apparatus, paraarticular tissues, and neurotrophic disorders.

Analysis of the therapeutic benefits of symptomatic slow acting drugs in osteoarthritis: emphasis on structural-modifying action

The review presents an analysis of the therapeutic effect in osteoarthritis (OA) of the original complex injectable drug Alflutop (bioactive concentrate of small marine fish), which is one of the most widely used symptomatic slow acting drugs (SYSADOA) in Russia. It stimulates the proliferation of chondrocytes, activates the synthesis of the extracellular matrix by modulating transforming growth factor β (TGFβ), inhibits hyaluronidase, oxidative stress and the activity of extracellular expression of proinflammatory cytokine genes – interleukin (IL) 1β, IL6 and IL8 in vitro.The results of prospective clinical studies are presented, which demonstrate the ability of Alflutop to slow down the X-ray progression of OA of the knee joints (inhibit the narrowing of the joint space, the growth of osteophytes and increase the intra-articular concentration of hyaluronic acid), as well as restore the mobility of the hip joints when it is locally introduced into the zone of pathological changes in the articular lip of the acetabulum. Combined therapy with Alflutop leads to activation of reparative processes and significant clinical improvement in patients with post-traumatic OA, and also slows down the progression of chondrodegeneration according to magnetic resonance imaging. The new Alflutop administration regimen for knee OA (2 ml every other day, №10) can increase patient adherence to therapy.The results of clinical studies presented in the review prove the structural-modifying effect of Alflutop in OA of various localization and substantiate its widespread use in this disease in rheumatological, traumatological and orthopedic practice.

Mesenchymal Stem Cells and Activation of Reparative Processes in the Brain and Retina

Mesenchymal Stem Cells and Activation of Reparative Processes in the Brain and Retina

Use of magnetotherapy in complex treatment and rehabilitation of patients with osteoarthritis (based on consensus of specialists for therapists and general practitioners of polyclinics)

Difficulties in treating patients with rheumatological diseases are due to the complexity and versatility of pathogenesis, frequent comorbidity and advanced age of the majority of such patients. The use of non-pharmacological methods of treatment, including physical methods, enhances the analgesic and anti-inflammatory effect, improves tissue trophism and promotes the activation of reparative processes, slows down the progression of the disease and generally improves the quality of life of patients. In the complex treatment and rehabilitation of rheumatological patients, the use of magnetotherapy seems to be a method that is well substantiated by the data of numerous fundamental and modern clinical studies.

EMBRYOGENESIS, LEUKOCYTIC PROFILE AND LIVER MORPHOLOGY OF PREGNANT RATES UNDER THE ACTION OF CITRIC ACID

The article presents the results of studies on the effects of citric acid on the female’s body. The studies were performed on white laboratory rats of the Wistar line, which at the age of 3 - 3.5 months with a body weight of 192 - 210 g were divided into two groups of 4 individuals in each. The animals of the experimental group were fed by citric acid at a concentration of 80 mg/l of drinking water during the period physiological and puberty, fertilization and pregnancy. A control group of female rats was fed by water. The animals had free access to drinkers and food. Indicators of embryogenesis, leukocyte profile of blood and body weight of animals, weighting factor, histological changes of the liver were determined.
 Female rats were found to have no abortions or premature births, however, the number of corpora lutea of pregnancy decreased by 19.2%, although there was no significant difference in control. The number of implantation sites per female was significantly lower by 28.8%. The mortality rate of embryos was 15.8%, which is 3.8 times higher than in intact animals. Regarding white blood cells, the analysis of the results indicates a tendency to increase the percentage of eosinophils in 2 times in the relation to the animals in the control group on the background of a probable increase in leukocytes by 42.4%.
 The applied dose of citric acid caused certain changes in the macro- and microstructure of the liver. It is macroscopically established that under the influence of citric acid the organ is slightly enlarged, sluggish consistency and changed its color from light red to light brown. The relative weighting factors of the liver are probably higher in the relation to the control (52.12 ± 8.16 g/kg vs 43.38 ± 1.96 g/ kg).
 The results of morphological studies indicate a partial violation of the lamellar structure of the organ in the centro-lobular region, which is due to turbid swelling of the cytoplasm of hepatocytes. In most cells, the contours are blurred, the cytoplasm is poorly stained, their nuclei are weakly basophilic, and hepatocytes with a lysed nucleus in a necrobiosis condition also occur. However, among dystrophic altered cells are clearly isolated hepatocytes with a uniformly colored basophilic cytoplasm, nucleus, rich in chromatin, which indicated the activation of reparative processes. Kupffer cells in this area are mostly round, located in the lumen of the sinusoid. Therefore, occurs a violation of lipoprotein complexes of cells and increase the permeability of cell membranes, which is reflected in the development of protein dystrophy with varying degrees of severity. Activation of reparative processes of hepatocytes is also determined.

Новая методика двустороннего самофиксирующегося шва при кесаревом сечении

Objective. To improve the technique for cesarean section and reduce the incidence of postoperative complications using selflocking surgical (anchor) sutures. Design and results. The study involved 86 patients who underwent cesarean delivery at the age from 18 to 40 years, similar in demographic parameters, somatic status, parity, pregnancy complications and the number of cesarean sections in history. Two groups of patients were allocated: the first (main) group – 46 women, in whom a double-sided single-row suture with a selflocking suture anchor without reverse tip was used to repair a wound on the uterus (patent RU2729747C1); the second (comparison group) – 40 women; a double-row suture to repair the uterine wound involving multifilament sutures was used. For a number of characteristics (the duration of operation, the duration of suturing of the uterus, the restoration of physical activity during the first day, the need for analgesics, the activity of reparative processes in the myometrium), the advantage of a singlerow suture anchor was noted. Conclusion. The developed method provides conditions for the activation of reparative processes in the myometrium and potentiates rapid rehabilitation of patients after surgery. However, further research is needed to introduce the method into widespread clinical practice. Key words: cesarean section, incompetent uterine scar, isthmocele, double-sided single-row suture, self-locking suture anchor

Complex treatment of experimental purulent wound of soft tissues

Objective. To study the impact of estrogens and liposomes on course of healing process in purulent wounds of soft tissues in experiment.
 Маterials and methods. Еxperimental investigation was done on 21 sheep. In the laboratory animals purulent-inflammatory process was simulated. Тhe laboratory animals were distributed into two groups: a control - 6 sheep and investigated - 15. All the animals obtained conventional therapy. In laboratory animals of the investigated group estrogens and liposomes were added to complex of the conventional treatment measures. The healing process course was controlled, using cytological, biochemical, planimetric and mathematic-statistical methods of investigation.
 Results. Application of combined estrogen-liposomal therapy in complex treatment of experimental purulent wound of soft tissues have enhanced the levels of оxyprolene, ribonucleic and deoxyribonucleic acids in the wound area, raised a value of estradiol/testosteronic index, accelerated the wound cleansing, the granulations creation and shortened the wound healing process by (2.12 ± 0.24) days.
 Conclusion. Including of estrogens and liposomes into the treatment complex for experimental purulent wound of soft tissues enhances cellular biosynthetic activity, activity of reparative processes in the wound area, accelerates the wound cleansing and the granulation creation, shortens the wound healing process and may be applied for improvement of the purulent wounds treatment in elderly and senile patients.

Abstract 164: Alteration of DNA methylation, DNA repair and epithelial-mesenchymal transition in colorectal cancer cell lines by S-adenosylmethionine treatment

Abstract Global DNA hypomethylation can be observed along with the aging of normal cells, and it is also related to tumor initiation and progression. S-adenosylmethionine (SAM) is a universal methyl donor molecule, used as a dietary supplement. SAM is involved in DNA methylation processes, thereby it may have a favorable effect on gene expression of cancer-associated genes through epigenetic modifications, but may also influence DNA folding during repair processes. Our aim was to analyze the effect of SAM treatment on global and promoter-specific DNA methylation level, gene expression, DNA integrity, cell cycle and the proliferation of two different colorectal cancer cell lines (HT-29, SW480). HT-29 and SW480 cells were treated with SAM in different concentrations (0, 0.5, 1 mmol/l) for 48 hours. Global DNA methylation status was analyzed by bisulfite pyrosequencing of long interspersed nuclear element-1 (LINE-1) retrotransposons. Promoter-specific DNA methylation alterations were determined by Reduced Representation Bisulfite Sequencing (RRBS) method. Gene expression changes were detected using Human Transcriptome Array 2.0 (HTA 2.0). DNA integrity analysis was performed with γH2AX ELISA, immunostaining and Comet Assay. Flow cytometry measurement and Sulforhodamine B (SRB) assay were assessed for cell cycle and proliferation determination. Global and promoter-specific DNA methylation alterations, as well as decreased expression (p< 0.05) of genes, that are involved in epithelial-mesenchymal transition were observed after SAM treatment. Increased phosphorylation of H2AX (74.9, 166.5, 200.6 pM) and decreased micronucleus number (1.47, 0.76, 0.45% of cells) were referred to the activation of reparative processes, that was supported by the changes of comet tail lengths. Proportion of cells was decreased in the G0/G1 (48.4, 28.5, 20.4%) phase; however, it was increased in both S (45.7, 61.7, 67.0%) and G2/M (6.0, 10.7, 12.5%) phases. Significant (p< 0.05) decrease of cell proliferation (99.5, 78.0, 70.6%) was also detected with SRB assay. SAM is able to alter the DNA methylation pattern of tumor cells and can induce DNA repair. Activation of these processes can lead to cell cycle arrest, decreased proliferation, and inhibition of epithelial-mesenchymal transition. Tumor cells could be targeted by SAM through different pathways; therefore, it may enhance the effect of chemotherapeutic agents. Citation Format: Sára Zsigrai, Alexandra Kalmár, Krisztina Andrea Szigeti, Zsófia Brigitta Nagy, Barbara K. Barták, Gábor Valcz, Orsolya Galamb, Titanilla Dankó, Zsolt Tulassay, Péter Igaz, Béla Molnár. Alteration of DNA methylation, DNA repair and epithelial-mesenchymal transition in colorectal cancer cell lines by S-adenosylmethionine treatment [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 164.

Glucagon mechanisms of therapeutic and prophylactic action of drinking mineral waters

Glucagon is one of the main hormones that regulate energy production, but its role in the implementation of the therapeutic and preventive potential of drinking mineral waters has not been sufficiently studied. Many of the effects of glucagon are related to the insulin supply of carbohydrate and lipid metabolism, and insulin takes an active part in the mechanism of action of mineral waters on various functional systems of the body. In an experiment in healthy animals, the stimulating effect of medium-mineralized water on the secretion of glucagon and the concentration of lipid precursors of enegretic metabolism was proved. It was found that activation of reparative processes and inhibition of acidic gastric secretion in rats with experimental gastroduodenal ulcer are associated with an increase in glucagon secretion under the influence of course intake of mineral water. It was found that in the case of metabolic syndrome, rats develop circadian rhythm disorders in the system of glucagon regulation of energy-homeostasis, which can be compensated by internal intake of mineral water. The hypothesis about the stimulating effect of drinking mineral waters on the interconnected insulin-glucagon regulation of the energy supply of sanogenetic reactions is analyzed.

Experience of using atraumatic ointment bandages based on peruvian balsam in the complex treatment of patients with chronic wound defects of the lower limbs

The article reflects the results of a retrospective non-comparative study, the objects of which were 34 outpatient comorbid patients (15 of which are over 60 years old) with chronic wounds of the lower limbs developed against the background of varicosity, post-thrombotic disease, chronic arterial insufficiency of the lower limbs, diabetes mellitus or their combination. Their local treatment was carried out using atraumatic ointment dressing «Branolind N» containing Peruvian balsam. There were 23 women (67.6 %) and 11 men (32.4 %). In microbiological study prior to the beginning of treatment in 31 patients the growth of a pathogen of wound infection was revealed; in 19 patients — Staphylococcus aureus in monoculture and in various associations, in 6 patients — Pseudomonas aeruginosa in monoculture, in other cases — other pathogens. In 3 patients the pathogen was not detected. The average wound size was 34 cm2 . The phase of the wound process was additionally confirmed by cytological studies. After treatment the average area of the wound defect decreased by 10 cm2 and made 24 cm2 on average. Full healing of the wound defect occurred in 11 patients, the others had granulation and active marginal epithelization. There was also a decrease in bacterial semination of wounds, a change in composition of infection agents to less aggressive monoflora, and cytologically — a decrease in signs of inflammation against the background of significant activation of reparative processes.

Application efficiency of complex pharmacotherapy schemes at combined mycotoxicosis in young bulls

The article presents data on the study of the effectiveness of tetra-p feed additive and toxin adsorbents Minazel plus and Sorbitox introduced into feed, naturally contaminated with mycotoxins. The experiment was conducted on young bulls of 9 months age, which for 3 months got the preparations in diets in the following concentrations: the first group – tetra-p – 5.0 kg/t of feed, Minazel plus – 1.5 kg/t of feed, the second group – tetra-p – 5.0 kg/t of feed, Sorbitox (1.5 kg/t of feed). The results of the experiment determined that the use of tetra-p in combination with preparations with sorption activity had a pronounced therapeutic effect on the body of young bulls. An improvement in the clinical condition of animals was observed from 14-16 days of the experimental period and was manifested by an increase in appetite, normalization of the gastrointestinal tract, as well as by an increase in body weight gain by 11.7 % and 10.3 %. Blood biochemical parameters were characterized by an increase in the level of total protein by 12.4–15.8 % (p ≤ 0.01), albumin by 18.0–21.3 %, urea by a factor of 1.53–1.44 (p ≥ 0.01), cholesterol by 1.4–1.56 times and triglycerides by 1.6 times. The activity of liver enzymes decreased by 18.3–21.4 % (ALT) and by 25.7–27.1 % (AST). It has been proved that the developed scheme improves the effectiveness of treatment of mycotoxicosis in animals by the optimizing effect on metabolism, restoration of impaired homeostasis, activation of reparative processes of the liver tissue and increased deposition, activation and utilization of pollutants, produced by microscopic fungi.

Evaluation of clinical efficacy of Wundahyl ointment in dermatology

Topical treatment of inflammatory skin diseases and trophic ulcers remains a serious problem in modern dermatology. The main difficulties in the treatment of wounds and inflammatory skin diseases are exacerbated by the complexity and variety of changes that occur during their healing. Of great importance in the treatment of diseases and skin lesions are drugs of natural origin.Objective — to determine the clinical efficacy of Wundahyl ointment in the treatment of inflammatory skin diseases.Materials and methods. This article contains the findings of the study of clinical efficacy of Wundahyl ointment in the treatment of 26 patients with inflammatory skin diseases. The age of the patients was from 12 to 63 years. 12 of them were men and 14 – women. The diagnoses of patients were psoriasis, eczema, plantar fissures, trophic ulcers, chronic ulcerative pyoderma and «clean wounds» after removal of the neoplasms.Results and discussion. The ointment was applied to the affected areas 2—3 times per day during 7—14 days. Additional treatment was not used. At 4—5 days, most patients noted a decrease in exfoliation and infiltration of the lesions. When using Wundahyl ointment in patients with chronic eczema of palms, eczema of fingertips, dry asteotic eczema, plantar cracks, coin­like eczema, pain was reduced on 2—3 days and epithelization of cracks was observed on 7—9 days.Conclusions. The results of the study indicate that Wundahyl ointment is an effective drug for the local treatment of inflammatory skin diseases of various origins. The drug provides activation of reparative processes (granulation and epithelization), is well tolerated and does not cause side effects.

Специфіка лікування деструктивних змін у верхівковому періодонті у хворих на туберкульоз легень

Introduction. The prevalence of a number of socially dangerous illnesses in Ukraine, including pulmonary tuberculosis, is quite high. Considerable groups of patients, in addition to anti-TB treatment, also require adequate dental care, taking into account the general somatic status.The aim of the study. To evaluate the effectiveness of the use of antibiotics in the endodontic treatment of destructive forms of apical periodontitis in patients with pulmonary forms of active tuberculosis.Materials and methods. An endodontic treatment of 23 teeth in 19 patients aged 21-39 years (30,6±5,7) with regard to destructive apical periodontitis has been performed. Therapeutic manipulations were carried out within the framework of the existing protocols. In addition to standard procedures, cotton swabs with 5% solution of kanamycin sulfate were introduced into the root canal to create the stores. In the control group aged 20 – 44 years (32,5±7,6) – 24 persons (25 teeth) with a similar dental pathology, treatment was carried out according to the standard scheme indicated in the protocol, an additional drug treatment of root canals with an antibiotic was not conducted. The control of long-term results was performed after 6 and 12 months according to clinical and radiological data.Study results and their discussion. The analysis of the immediate results confirmed the reduction in the number of complications requiring repeated endodontic interventions and other therapeutic care activities by 27% compared to the control group. According to digital dental radiography, the use of the complex of kanamycin sulfate + calcium-containing paste in endodontic treatment accelerates the processes of restoration of bone structures in the destruction zone and allows in 12 month period to restore periodontitis in 72.0% of cases.Conclusions. The inclusion of kanamycin sulfate in the protocol of endodontic treatment of destructive processes in apical periodontitis in patients with active forms of pulmonary tuberculosis improves the remote results due to the sanitation and prevention of the formation of specific infection centers in the maxillofacial area and activation of reparative processes.

Morphologic and Morphometric Parameters of the Liver Tissue of Laboratory Animals After Modeling of Chronic Stress

Objective: to study changes in the morphologic and morphometric parameters of the liver tissue in male Wistar rats under chronic Ortiz stress. Material and methods. The study was conducted on 50 male Vistor rats. The experimental group of the animals was subjected to 10-day chronic stress by Ortiz. The morphologic and morphometric parameters of the liver tissue of the animals exposed to chronic stress were studied. Results. The morphologic changes in the liver of the animals which had sustained chronic stress were characterized by impaired blood circulation and degenerative changes of hepatocytes. At the same time, a statistically significant increase in the percentage of binuclear hepatocytes (p = 0.046) and functional cariocellular index (p = 0.03) was found. Conclusion. The effect of chronic stress leads to a change in the morphologic and morphometric parameters of the liver tissue with activation of reparative processes.

Significant improvement in cervical repair after surgery under the influence of specific anti-inflammatory therapy

The article describes the features of the management of patients with cervical dysplasia. After the surgical treatment of cervical neoplasia, patients experience abundant serous or serous bleeding from the genital tract, which leads to marked discomfort and secondary infection of the cervix. Secondary infection contributes to the prolongation of epithelialization of the cervix and the occurrence of a relapse of the disease.It emphasizes the importance of the prevention of postoperative symptoms that cause discomfort in women, secondary infection of the wound surface and prolongation of epithelialization of the cervix. These symptoms occur after trauma to the cervix due to an increase in prostaglandins, histamine and pro-inflammatory cytokines.The study showed that benzydamine hydrochloride reduces the abundance and duration of postoperative secretions from the wound surface by suppressing tissue edema and inhibiting the synthesis of proinflammatory cytokines – tumor necrosis factor and interleukin-1, which leads to increased reparative processes of the cervix epithelium. Benzydamine hydrochloride reduces capillary permeability, stabilizes lysosomal membranes, inhibits the production of adenosine triphosphate and other high-energy compounds in the processes of oxidative phosphorylation, inhibits the synthesis or inactivate prostaglandins, histamine, bradykinin, cytokines, complement factors and other endogenous non-specific “damaging factors”. It blocks the interaction of bradykinin with tissue receptors, restores impaired microcirculation and reduces pain sensitivity in the focus of inflammation, also affects the thalamic pain sensitivity centers (local blockade of PgE1, PgE2 and PgF2 alpha synthesis). The analgesic effect is due from indirect decrease in the concentration of biogenic amines, which have algenic properties, and an increase in the pain sensitivity threshold of the receptor apparatus.Thus, benzydamine hydrochloride Tantum Rosa® is the only non-steroidal anti-inflammatory agent for topical use in gynecology, which provides rapid epithelialization of the cervix and elimination of pathological secretions due to the specific inhibition of pro-inflammatory cytokines and activation of reparative processes in the epithelium.

DYNAMICS OF MORPHOLOGICAL CHARACTERISTICS OF THE WOUND PROCESS IN PATIENTS WITH ODONTOGENIC FLEGMONS IN ANTIOXIDANT THERAPY

Aim. This research was designed to conduct a morphological analysis of the purulent wound exudate in the complex treatment of patients with odontogenic phlegmon of maxillofacial area (MA) by the antioxidant “Rexod” and antihypoxant “Mafusol”.Materials and methods. The objects of the study were 62 patients (aged 18 to 50 years) with odontogenic phlegmon who were divided into 3 groups. Group I (n=20) – the traditional treatment was supplemented by the intravenous drip injection of the antioxidant "Rexod"; group II (n=21) – the traditional treatment was combined with the intravenous injection of the antioxidant "Rexod" and antihypoxicant "Mafusol"; Group III (n=21) had the traditional treatment. The debridement of the purulent wound included a treatment with 0.06% sodium hypochlorite solution in all groups of patients in the first phase of the wound process; in the second phase the patients in all main groups were treated with the "Soderm" ointment dressings; in the comparison group were used the bandages with methyluracil ointment. In the third phase the gel "Kontraktubeks" was used to prevent the formation of rough scar tissue. There was carried out a morphological examination of smearsimprints of the wound exudate.Results. The microscopic analysis of the cellular landscape of the exudate revealed a significant number of polynuclears which are probably the segmented neutrophilic leukocytes and typical eosinophils; the number of mononuclear cells represented by typical lymphocytes was 4 times less; a single presence of macrophages, plasmocytes, fibroblast-like and epithelioid cells. The signs of degeneration were identified in the cells of histiogenic origin.Conclusion. As a result, there was revealed a gradual increase of overall amount of cells in the wound exudate in all study groups during the treatment, however, this process was pronounced to a greater extent in the patients of group I and II. The obtained result indicates the direct dependence of the activation of reparative processes in the wound from the applied medicines.

Репаративные процессы при алло- и ксеноимплантации внеклеточного матрикса кости

В настоящее время потребность в костно-пластическом материале возрастает при проведении реконструктивно - восстановительных операций на костной ткани в травматологии и ортопедии, челюстно-лицевой хирургии, костной онкологии и в других случаях хирургической практики. Среди имплантационных материалов на основе костной ткани лидирующее место занимают импланты ауто- и аллогенного происхождения. Аутогенные импланты - лучшие с биологической точки зрения, но их достойной альтернативой являются материалы из ксенокости в силу своей доступности и биосовместимости. Цель исследования - изучение репаративных процессов в зоне регенерации при алло - и ксеноимплантации материала, полученного из костной ткани, в полуциркулярный дефект диафиза бедренной кости крыс. Материалы готовили по одинаковой технологии. Методика. Биохимические и морфологические исследования проведены на 18 крысах-самцах линии Вистар в возрасте 6-8 месяцев. Животные были распределены на 3 группы. Первая группа (n = 6) - ксеноимплантация, вторая (n = 6) - аллоимплантация, третья контроль (n = 6) - здоровые животные. Изучены биохимические показатели сыворотки крови на 60-е сут. эксперимента: активность общей щелочной фосфатазы и тартратрезистентного изофермента кислой фосфатазы, содержание кальция, фосфата, общего белка, С-реактивного белка, белковых фракций и проведены морфологические исследования. Результаты и обсуждение. Биохимические исследования белковых фракций и С-реактивного белка сыворотки крови свидетельствуют об отсутствии выраженной воспалительной реакции у крыс при алло - и ксеноимплантации внеклеточного матрикса костной ткани в области дефекта метафиза бедренной кости. В обеих опытных группах отмечена активация репаративных процессов. Гистологический анализ выявил остеоинтеграцию как аллогенных, так и ксеногенных фрагментов губчатой кости, имплантированных в область полуциркулярного дефекта бедренной кости крыс. При использовании аллоимплантатов отмечен более высокий темп их биодеградации и органотипическая перестройка оперированного участка кости реципиента. Во всех случаях показано отсутствие реакции отторжения либо инкапсуляции импланта и признаков воспаления в тканях реципиента. Полученные нами данные биохимического и гистологического исследований являются доказательством возможности использования внеклеточного матрикса ксеногенной природы для замещения дефектов костной ткани.The need for bone-plastic material is presently rapidly increasing, particularly for reconstructive and restorative surgeries on bone tissue in traumatology and orthopedics, maxillofacial surgery, bone oncology, and other cases of surgical practice. Among the implantation materials based on bone tissue the leading place is occupied by auto- and allogeneic implants. Autogenic implants are the best from the biological point of view but xenobone materials are their worthy alternative due to their availability and biocompatibility. The aim of this research was to study reparative processes in the regeneration zone during allo- and xenoimplantation of a material obtained from bone tissue into a semicircular defect in the rat femoral diaphysis. The materials were prepared using the same technology. Methods. Biochemical and morphological studies were performed on 18 male Wistar rats aged 6-8 months. The rats were divided into 3 groups. The first group (n = 6), xenoimplantation; the second group (n = 6), alloimplantation, and the third group, healthy control (n = 6). On the 60 day of experiment, biochemical indexes of blood serum were studied, including activities of total alkaline phosphatase (TCA) and tartrate-resistant isoenzyme of acid phosphatase (TrAP), calcium, phosphate, total protein (TP), C-reactive protein, protein fractions, and morphological studies. Results. Measuring serum protein fractions and C-reactive protein showed the absence of a significant inflammatory reaction to allo- and xenoimplantation of extracellular bone matrix into the defect of femoral metaphysis. Activation of reparative process was noted in both experimental groups. Histological analysis found osteointegration of both allogeneic and xenogenic fragments of the spongy bone implanted into the semicircular defect of rat femur. When alloimplants were used a high rate of their biodegradation and organotypic remodeling of the operated recipient’s bone were observed. However, in all cases, there was no evidence of a rejection reaction, encapsulation of the implant or inflammation in tissues of the maternal bed.

STUDY OF REPARATIVE PROCESSES OF BONE TISSUE IN RATS USING OSTEOPLASTIC MATERIAL KLIPDENT PL WITH MEMBRANE KLIPDENT MK AND EASY-GRAFT MATERIAL WITH HYPROSORB MEMBRANE

Acceleration of the reparative process of bone tissue after surgical interventions is an urgent issue in surgery and dentistry. One of the methods for replacing lost bone tissue is using an osteoplastic material. There is a question in the choice of better material, which will meet all the requirements of the doctor and fully perform its function. This work studies the activity of reparative processes of bone tissue in rats when the titanium screw is screwed into the femur using synthetic osteoplastic material Klipdent PL with membrane Klipdent MK (“Vladmiva” Russia) and Easy-graft material with Hyprosorb (“Sunstar” Swiss) membrane. The obtained results were compared with a control group in which the similar manipulation was performed without the use of an osteoplastic material. The experimental and morphological study was carried out. Conclusions are drawn about the effectiveness of the materials under study.