Event Abstract Back to Event Quality controls of Cell-Based therapies: Assessment of conditioned Dendritic Cells phenotypes by Global Gene Expression Profiles Samantha Baldassarri1, Chiara Ripamonti1, Silvia Fumagalli1 and Maria Foti1, 2* 1 University of Milano-Bicocca, Department of Surgery and Interdisciplinary Medicine, Italy 2 Genopolis, Department of Biotechnology and Bioscience, Italy Whole-cell immunotherapies have shown promising results in clinical trials. Nevertheless, the complex nature of the cellular product and the individual variability associated to their preparation makes cellular immunotherapies less well characterized. Therefore, one major challenge in the development of whole cell therapies is the ability to demonstrate a clear comparability of the cellular product obtained after the application of different differentiation settings and conditioning protocols in the manufacturing process. Research in our lab is focused towards the development of novel molecular assays that can be used as quality controls in cell-based therapies. Using a functional genomics approach we have been able to select a genetic signature of activation of DCs in vitro. We selected lists composed of 44 genes able to discriminate different DCs phenotypes. We validated the signature in vivo in DCs derived from a mouse model of inflammation. The selected signature has been further validated in human derived DCs in order to develop a quality control molecular assay for DCs-based therapies. Therefore, the basic methods to select a genetic signature of DCs that may predict in vivo functional activity and its applications in cellular immunotherapies are presented. Acknowledgements Italian Foundation for Multiple Sclerosis. FISM2009 Italian Ministry of University and Scientific Research, PRIN2009 7FP Project: FIGHT-MG 242210. 7FP Project: TOLERAGE HEALTH-F4-2008-202156. Keywords: Dendritic Cells, Immunotherapy, Gene Expression Profiling, Cell based therapies, Immune Regulation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Baldassarri S, Ripamonti C, Fumagalli S and Foti M (2013). Quality controls of Cell-Based therapies: Assessment of conditioned Dendritic Cells phenotypes by Global Gene Expression Profiles. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00915 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Maria Foti, University of Milano-Bicocca, Department of Surgery and Interdisciplinary Medicine, Milan, Italy, maria.foti@unimib.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Samantha Baldassarri Chiara Ripamonti Silvia Fumagalli Maria Foti Google Samantha Baldassarri Chiara Ripamonti Silvia Fumagalli Maria Foti Google Scholar Samantha Baldassarri Chiara Ripamonti Silvia Fumagalli Maria Foti PubMed Samantha Baldassarri Chiara Ripamonti Silvia Fumagalli Maria Foti Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.