Action Of Additives Research Articles

Simulation study on additive-promoted microwave dehydration of lignite

ABSTRACT Microwave dehydration technology has been widely used in coal processing methods due to its unique advantages. Adding additives with high dielectric loss to coal can enhance the microwave absorption capacity of coal, so as to effectively improve the dehydration process. In order to understand the change and distribution of multi-fields in the microwave drying process of lignite under the action of different additives, verify the experiment and reduce the cost and number of experiments, this paper established a multi-field coupling model of electromagnetic field and heat and mass transfer of multiphase porous media based on COMSOL Multiphysics, so as to simulate the microwave drying process of lignite. The results show that the distribution areas of electromagnetic field, temperature field, and vapor in coal are basically the same, while the distribution of liquid water is just the opposite. With the enhancement of additive promotion effect, the parts with strong electromagnetic field strength increase, the hot spots of temperature field increase, the maximum temperature, and minimum temperature increase, and the water content in coal samples decreases. The promotion effect of additives on the microwave dehydration process of lignite is consistent with the experiment.

Organic Electrolyte Additives for Aqueous Zinc Ion Batteries:Progress and Outlook.

Aqueous zinc ion batteries (AZIBs) are considered one of the most prospective new-generation electrochemical energy storage devices with the advantages of high specific capacity, good safety, and high economic efficiency. Nevertheless, the enduring problems of low Coulombic efficiency (CE) and inadequate cycling stability of zinc anodes, originating from dendrites, hydrogen precipitation and passivation, are closely tied to their thermodynamic instability in aqueous electrolytes, which significantly shortens the cycle life of the battery. Electrolyte additives can solve the above difficulties and are important for the advancement of affordable and reliable AZIBs. Organic electrolyte additives have attracted widespread attention due to their unique properties, however, there is a lack of systematic discussion on the performance and mechanism of action of organic electrolyte additives. In this review, a comprehensive overview of the application of organic electrolyte additives in AZIBs is presented. The role of organic electrolyte additives in stabilizing zinc anodes is described and evaluated. Finally, further potential directions and prospects for improving and directing organic electrolyte additives for AZIBs are presented.

Clarifying the mechanism of actions of suppressor, brightener and sodium chloride in acid copper electrodeposition: A multidisciplinary approach for a many variable problem

Acid copper (AC) electrodeposition still a cardinal process for many fields, from electronic to decorative industries. Moreover, AC electroplating is a necessary step for Ni-free production cycles which are trending given the increased awareness of the toxicity of Ni2+ salts. State-of-the-art AC baths are formulated with a set of three organic additives, which are needed to achieve coatings with proper mechanical and optical features. However, despite the widespread use of these substances, their mechanism of action and the role of NaCl during the deposition are not clearly defined. A multidisciplinary approach has been employed to obtain insights on the mechanism of action of the organic additives on the Cu surface. This approach is based on electron microscopy electrochemical analysis supported by X-ray based techniques and computational calculations and simulations. An electrical field was included in molecular dynamics to delve the interactions between active species within the electrodeposition.

Revealing the Limitation Induced by Hydroxyl in Regulating Solvation Structure of Zn2+ and Overcoming Challenges with Hybrid Additives towards Highly StableZinc Anodes.

In the field of electrolyte design for aqueous zinc-ion batteries (AZIBs), additives containing hydroxyl have been demonstrated to effectively modulate the solvation structure of Zn2+. However, reported studies typically focus solely on the effectiveness of hydroxyl while neglecting the issues that emerge during solvation structure regulation. The strong electron-attracting capability of Zn2+ attracts electrons from the oxygen in hydroxyl, thereby weakening the strength of hydroxyl, the hydrogen evolution reaction (HER) is also pronounced. This work innovatively reveals the limitation of hydroxyl-containing additives and proposes a synergistic regulation strategy based on hybrid additives. Arginine with a high isoelectric point is introduced into the electrolyte system containing hydroxyl additives. The protonation effect and electrostatic attraction of arginine enable it to absorb protons at the anode released by the weakened hydroxyl, thereby compensating for the limitation of hydroxyl additives. Under the synergistic action of hybrid additives, the Zn|Zn battery achieved stable deposition/stripping for over 1200 hours under 10 mA cm-2 and 10 mAh cm-2. Moreover, the Zn|Cu battery cycled for over 570 hours with a high Coulombic efficiency of 99.82%. This study presents a pioneering perspective for the further application of AZIBs.

Efficiency of the Action of Multifunctional Additives in Lubricating Oils

Efficiency of the Action of Multifunctional Additives in Lubricating Oils

Chicken intestinal organoids: a novel method to measure the mode of action of feed additives.

There is a rapidly growing interest in how the avian intestine is affected by dietary components and feed additives. The paucity of physiologically relevant models has limited research in this field of poultry gut health and led to an over-reliance on the use of live birds for experiments. The development of complex 3D intestinal organoids or "mini-guts" has created ample opportunities for poultry research in this field. A major advantage of the floating chicken intestinal organoids is the combination of a complex cell system with an easily accessible apical-out orientation grown in a simple culture medium without an extracellular matrix. The objective was to investigate the impact of a commercial proprietary blend of organic acids and essential oils (OA+EO) on the innate immune responses and kinome of chicken intestinal organoids in a Salmonella challenge model. To mimic the in vivo prolonged exposure of the intestine to the product, the intestinal organoids were treated for 2 days with 0.5 or 0.25 mg/mL OA+EO and either uninfected or infected with Salmonella and bacterial load in the organoids was quantified at 3 hours post infection. The bacteria were also treated with OA+EO for 1 day prior to challenge of the organoids to mimic intestinal exposure. The treatment of the organoids with OA+EO resulted in a significant decrease in the bacterial load compared to untreated infected organoids. The expression of 88 innate immune genes was investigated using a high throughput qPCR array, measuring the expression of 88 innate immune genes. Salmonella invasion of the untreated intestinal organoids resulted in a significant increase in the expression of inflammatory cytokine and chemokines as well as genes involved in intracellular signaling. In contrast, when the organoids were treated with OA+EO and challenged with Salmonella, the inflammatory responses were significantly downregulated. The kinome array data suggested decreased phosphorylation elicited by the OA+EO with Salmonella in agreement with the gene expression data sets. This study demonstrates that the in vitro chicken intestinal organoids are a new tool to measure the effect of the feed additives in a bacterial challenge model by measuring innate immune and protein kinases responses.

Features of ignition and combustion of low-grade coal mixed with pyrolysis liquid

THE RELEVANCE of the study lies in the need to use pyrolysis liquid from wood processing for combustion in boilers of medium and low power as part of mixtures with coal. The unpredictability of the ignition and combustion properties of such mixtures, as well as the lack of calculation methods, predetermined a predominantly experimental approach to the study of these processes. At the same time, for the widespread implementation of such technological solutions in practice, it is necessary to have relatively simple methods for assessing the ignition and combustion characteristics of these components. THE PURPOSE. Synthesize samples of pyrolysis liquid from two different sorts of wood and perform their characterization. Develop an experimental method for studying ignition and combustion characteristics of pyrolysis liquid mixed with low-reactivity coal. Perform research on ignition delay times and the composition of released gas-phase products. Determine the main mechanisms of action of the components in the mixture of low-grade coal with pyrolysis liquid they’re during ignition and combustion. METHODS. The study was carried out using an experimental technique based on a high-temperature muffle furnace equipped with a high-speed video camera and a once-through gas analyzer. RESULTS. The article presents the dependences of the ignition delay times and the concentration curves of gas-phase products released during combustion of mixtures of low-grade coal and two types of pyrolysis liquid in the temperature range of the heating medium 600-800 °C and additive concentrations from 0 to 20 wt.%. Linear approximation dependences of ignition delay times on additive concentrations were obtained, and the mechanism of action of the additive during combustion was formulated. CONCLUSION. It was found that an increase in the concentration of the additive in the mixture leads to a stable decrease in the ignition delay times, while the amount of gas-phase products formed varied additively with respect to the composition of the mixture. The shape of the concentration curves for the release of CO and NOx indicates the independent action of the components of the mixture. The mechanism of action of additives was proposed. It was based on significant contribution of their evaporation process followed by combustion in the gas phase, which, in turn, initiats the ignition of coal.

The Role of Chemical Activation in Strengthening Iron Ore Tailings Supplementary Cementitious Materials

The preparation of iron ore tailings (IOTs) into supplementary cementitious materials (SCMs) is an effective approach to achieve value-added utilization of industrial solid waste. This study systematically investigates the hydration pattern and strength development of Portland cement systems with the incorporation of IOTs, steel slag (SS), granulated blast-furnace slag (GBFS), and fly ash (FA) under the action of different chemical additives. The hydration products, and microstructure and pore structure of the SCMs are analyzed using X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and mercury intrusion porosimetry. The findings of this study demonstrate that chemical activation plays a significant role in the strength development of SCMs. Among the five chemical activators tested, Triethanolamine (TEA) had the greatest influence on mechanical properties. The maximum compressive strength of the SCMs at 28 days was 42.9 MPa at a dosage of 1%. Specifically, the addition of TEA promotes volcanic ash reactions, and the high fineness of SCM provides nucleation sites for hydration products. Interactions between the volcanic ash reaction and the complexation reaction of TEA have a positive effect on compressive strength development. This research expands the potential for IOTs SCMs through chemical activation methods for value-added applications.

Experimental and numerical study of the mixed lubrication under the action of magnetic ionic liquid additives

In this paper, the tribological characteristics of an oil-soluble magnetic fluid additive under mixed lubrication are studied by experiments and numerical simulation. [bmim][FeCl4] is dissolved in CF10W-40 lubricating oil as a magnetic liquid additive, and its friction coefficient is tested by a point contact friction tester at different temperatures, rotational speeds and magnetic field intensities. The transition condition of lubrication state is obtained through analyzing the Stribeck curves based on the experiments, and the strength model of boundary film is established accordingly. A mixed lubrication model is established by substituting the boundary film strength model and the surface roughness model into the hydrodynamic lubrication model based on Reynolds equation. The results show that the magnetic solution as an additive can obviously reduce friction and wear, and the effect is more obvious under the condition of magnetic field. The boundary film strength model can accurately reflect the transition characteristics of lubrication state in the presence of boundary film, and the mixed lubrication model based on boundary film strength model can more precisely reflect the tribological characteristics of friction pairs, so this study provides a new theoretical method for the related research on the influence of boundary film on lubrication characteristics.

Peculiarities of morphological indicators of quail spleen (Coturnix japonica Temminck et Schlegel, 1849) when using protein-vitamin mineral supplements

In most countries of the world, poultry farming is one of the main branches of agriculture, which provides the population with high-quality food products. To date, the positive dynamics of the growth of such a branch of industrial poultry farming as quail breeding has been noted. Quails have a complex of excellent economic and productive advantages compared to other agricultural birds – high body temperature, intensive metabolism in the body, small size, precocity, high egg productivity. The question of increasing the productivity of quail breeding, as a branch of precocious animal husbandry, has always occupied the attention of biologists, morphologists, physiologists, geneticists and technologists. This topical problem has various scientific aspects: morphological, histochemical, physiological, genetic, etc. Special attention in the conducted research is required to study the impact of additives on the body of quails, the absence of negative effects and the production of safe products for humans. We chose the spleen as a biomarker, because the spleen is an important peripheral organ of immune protection, sensitive to the action of various factors, whose morphofunctional indicators determine the magnitude and nature of the impact. The purpose of the research was to determine the effect of modern protein-vitamin mineral supplements, which are advertised and offered by producers in the poultry industry to stimulate the growth and development of the bird, on the quail body. In the studies, the features of the microscopic structure and morphometry of the spleen of control and experimental animals were used as sensitive characteristics of the action of additives. The proposed methods are aimed at researching the impact of protein-vitamin mineral supplements on the relevant industry. So, for 21 days, mineral supplements “Multilife”, “Missy” and “Standard Agro” were added to the main ration of quails. Using macrometric methods, the body weight of quails, the weight of the spleen (absolute and relative) were determined. For histological studies, pieces of the spleen were fixed in a 10 % formalin solution, and after embedding in paraffin, histological sections were prepared from it, which after deparaffinization were stained with hematoxylin and eosin, according to the methods of Brachet and Van Gieson. For electron microscopic examination, the spleen was immediately fixed with a 2.5 % solution of glutaraldehyde in a phosphate buffer with additional fixation in a 1 % solution of osmium tetroxide according to Caulfield. For the first time, the ultrastructural organization of the spleen of Japanese quail was investigated after feeding protein-vitamin mineral supplements, which may indicate the absence of their negative impact on the structural and functional state of the spleen. Important morphological structural indicators of the morphofunctional state of the spleen were determined: the relative area of the white pulp, red pulp, lymphoid nodules, periarterial lymphoid sheaths, and the contractile apparatus. According to the analysis of the morphological results obtained by us, it was investigated that the microscopic structure of the quail spleen is characterized by the presence of a support-contractile apparatus with a relative area of 4.58 ± 0.76 % and single radial trabeculae. The parenchyma is formed by white and red pulp without clear boundaries. Lymphoid nodules without bright centers, periarterial lymphoid sheaths, and ellipsoids were identified as part of the white pulp. The ultrastructural organization of the spleen of quails had peculiarities: the nuclei of leukocytes contain large nucleoli, the endotheliocytes of the central artery of the lymphoid follicle perform a phagocytic function by accumulating autophagosomes in their cytoplasm. The macroscopic, microscopic and ultramicroscopic structure of quails that were added to the main diet with the listed additives did not undergo any changes. As a result, the following supplements led to an increase in the body weight of quails: by 5.78 % when using the Multilife supplement, by 12.51 % when using the Missy supplement, and by 8.12 % when using the Standard Agro supplement, and the weight of the spleen on 7.17 %, 16.04 % and 10.54 %, respectively. The conducted studies showed that the relative weight of the organ practically did not change. At the same time, there is a trend towards an increase in the relative area of the white pulp and the supporting-contractile apparatus. Taking into account the obtained data, it can be concluded that our research confirms the feasibility of using modern protein-vitamin mineral supplements in poultry farming to stimulate the growth and development of poultry. Especially valuable is the absence of a negative effect on the morphofunctional indicators of the spleen, which was used as a biomarker in research.

OP33 Mechanistic insights on the role of ultra processed foods as a trigger/fuel for IBD

Abstract Background Ultra processed foods (UPFs) are a heterogeneous category of products that contain food additives and/or have undergone several industrial processes. The higher intake of UPFs seen in industrialized countries has been associated with increased incidence of inflammatory bowel diseases (IBD), mainly Crohn’s disease (CD). However, the specific mechanisms through which the UPFs can induce or maintain intestinal inflammation are still unknown. Therefore, most dietary interventions for IBD essentially exclude all UPFs from the menu, increasing the difficulty of long-term compliance of these diets. The main focus of my research is the study of which components may be detrimental in UPFs, in the hope that we can limit the number of UPFs that we need to exclude from our diets. Methods To understand the mechanism of action of UPFs, a translational approach is needed with complementary pre-clinical and clinical studies. We used organoid cultures from patients with CD to test the impact of food additives on the intestinal epithelium (e.g. permeability, pro-inflammatory effect). These organoid cultures are more physiologically relevant than immortalized cell lines, which lack several important aspects (e.g. genetic background, complex assembly of different cells). The results of the first tests with this platform have been submitted to ECCO (EC24-1418). Additionally, we established several protocols and collaborations to run well designed dietary intervention studies to explore the mechanisms of action of these food additives in humans. In the FOAM study, we explored the effects of 5 emulsifiers in an double blind randomized placebo controlled trial. The results of the FOAM study have also been submitted to ECCO (EC24-0858). We also explored the acceptance, feasibility, and compliance of an emulsifier free diet in healthy volunteers (EC24-1181). Results Our results support the need to better understand the role of food additives and UPFs in IBD, and the need for better evidence supporting the detrimental role of food additives. Conclusion Compliance is an important issue to consider in all dietary interventions, more than in pharmacological interventions. Strategies focusing on improving the compliance of dietary interventions can have a big impact in the applicability of these interventions in therapeutic and/or prophylactic settings. However, the current knowledge on the possible detrimental role of UPFs does not allow to pinpoint which UPFs are detrimental and which might have no effect on intestinal inflammation. Hence, there is an unmet need for pre-clinical and clinical studies focusing on the mechanisms through which these UPFs might play a role in intestinal inflammation.

On the Difference in the Action of Anti-Wear Additives in Hydrocarbon Oils and Vegetable Triglycerides

On the Difference in the Action of Anti-Wear Additives in Hydrocarbon Oils and Vegetable Triglycerides

Mechanisms of chemical vapor generation by aqueous boranes for trace element analysis: a rather long and unfinished story

Abstract This paper reports a summary of the studies on the mechanisms that govern the generation of hydrides and other volatile species through reaction with aqueous boranes (CVG). This derivatization reaction has been used since the early 1970s for elemental determination and speciation from the trace level down to the ultratrace level and below. An IUPAC project, concluded in 2011, reported on the mechanisms of hydrogen transfer from borane to the analytical substrate and served to remove erroneous concepts that had dominated CVG until the early 2000s. Following the conclusion of the IUPAC project, many studies have been published on the mechanisms of CVG under conditions approaching those of analyzes of real samples. These studies included the definition of more general reaction models, which valid under non-analytical conditions, the mechanism of action of additives and interferences in CVG of volatile hydrides. In addition to the analytical utility for CVG, the results represent a contribution to the knowledge of the chemistry of aqueous boranes. Other studies will be necessary to clarify still unknown aspects, among them the identity of volatile transition metal species formed by reaction of metal ions with aqueous boranes.

282 Awardee Talk: Effects of Various Feed Additives on Finishing Pig Feed Efficiency: A Review

Abstract Several feed additives have shown benefits throughout the literature in improving growth performance and carcass characteristics when fed to finishing pigs. The mechanisms of action of these feed additives may be by improving health status, energy metabolism, and/or nutrient digestibility. However, the results have been variable and have not been well summarized. In addition, few of the feed additives have a review of all the available literature, and to our knowledge, no review summarizes each feed additive in one centralized effort. Therefore, this review summarizes the available research on feed additives fed in swine diets for the grow-finish phase (Table 1). The feed additive categories chosen for this review were acidifiers, betaine, Cr, conjugated linoleic acid (CLA), Cu, direct-fed microbials (DFM), carbohydrases, proteases, phytases, multi-enzymes, essential oils (EO), L-carnitine, yeasts, and Zn. Research articles were collected from online research databases. Inclusion and exclusion criteria were utilized to select qualified research articles. The percentage difference for each response variable between the treatment and control group was calculated to determine the magnitude of effect. Results indicated that positive outcomes for each feed additive can be found; however, the magnitude of improvement varied, and many experiments did not observe statistically significant improvements in response criteria. For G:F, acidifiers (n = 65 total comparisons, 3.1% improvement across all comparisons), betaine (n = 35, 2.7%), CLA (n = 57, 3.5%), multi-enzymes (n = 29, 3.3%), DFM (n = 66, 3.3%), L-carnitine (n = 24, 2.5%), and yeasts (n = 33, 2.7%) showed relatively large and positive effects (> 2%) across a reasonable number of comparisons (n > 20) with > 14% of them being statistically ( P≤ 0.10) positive. For ADG, DFM (n = 71, 3.3%), Cu (n = 155, 2.5%), L-carnitine (n = 24, 2.1%), and multi-enzymes (n = 29, 3.1%) showed relatively large and positive effects (>2%) across a reasonable number of comparisons (n >20) with > 21% of them being statistically (P ≤ 0.10) positive. Moreover, except for betaine, Cr, CLA, and L-carnitine, most feed additives showed little, inconsistent, or non-significant effects on carcass characteristics. The improvements in carcass characteristics were probably due to the improved energy metabolism. Publication bias and the location of studies need to be considered when evaluating the data, as many studies that show no effect might not be published. Furthermore, even though the economics was not discussed in this review, the decision to include feed additives in the pig diets should consider the return on investment based on the price and the magnitude of benefit. In conclusion, this literature review provides descriptive analysis and databases for commonly used feed additives in the hope of better understanding the effect of feed additives on improving the efficiency of swine production.

Study on phase transformation and lithium extraction of amblygonite using low temperature acid free roasting method

The demand for lithium resources is constantly increasing with the vigorous development of the new energy industry. Extracting lithium from various lithium containing resources through green and environmentally friendly methods has become a research hotspot and has attracted widespread attention. A method for extracting lithium from amblygonite by low-temperature acid free roasting combined with mild leaching is proposed in this paper. The lithium extraction process of amblygonite was studied, and its phase composition and phase transition behavior during calcination were discussed in detail. The thermodynamic analysis by HSC 6.0, series of experimental investigation, and characteristics tests on the roasting product by XRD were carried out. The research results indicate that the transformation of LiAl(PO4)(F, OH)→AlPO4, LiAlSi2O6, and LiF in amblygonite can be achieved through acid free roasting process. Under the action of chloride additives, this phase transformation process will be promoted, and the lithium extraction rate will also be improved. After being chlorinated and roasted at 850 °C, amblygonite is leached by HCl with a concentration of 0.6 mol/L, a liquid-solid ratio of 4 mL/g, a temperature of 95 °C, and a reaction time of 1.5 h. The lithium extraction rate can exceed 96%. The external dependence on spodumene mineral resources can be alleviated, which has a positive effect on promoting the green and healthy development of the lithium battery industry.

Research on Electroplating Bonding of Flip-Chip Under the Action of Additives

Research on Electroplating Bonding of Flip-Chip Under the Action of Additives

Assessment of injector-flow characteristics of additised and renewable diesel blends through high-speed imaging

The influence of additives inducing viscoelasticity in diesel fuel, on the in-nozzle cavitation evolution and the expelled spray morphology has been quantified by high-speed, diffused back-light and schlieren imaging applied to two single-hole true-scale transparent injectors of straight and tapered orifice layouts (so-called Spray C and D of the engine Combustion Network), as well as a five-hole configuration (Spray M). More specifically, the in-nozzle cavitating flow and its effect on near-nozzle spray formation of a non-Newtonian diesel fuel sample treated with Quaternary Ammonium Salt (QAS) additives and exhibiting viscoelastic effects, as well as biodiesel (FAME), are compared against conventional diesel fuel for the first time. The operating conditions corresponded to injection and ambient pressures in the range of 500–900 bar and 1–20 bar, respectively. It was found that viscoelasticity has an overall suppressing effect on wall-attached, or so-called geometrical, cavitation. Furthermore, the investigation revealed that the action of viscoelastic additives has the capability to enhance the magnitude of well-established longitudinal vortices, with the subsequent after-effect of leading to increased cone angles of the expelled spray. On the contrary, it tends to suppress turbulence-induced transient instabilities in a manner similar to turbulence suppression.

Regulating SEI Components of Sodium Anode via Capturing Organic-Molecule Intermediates in Ester-Based Electrolyte.

Highly reversible sodium metal anodes are still regarded as a stubborn hurdle in ester-based electrolytes due to the issue of uncontrollable dendrites and incredibly unstable interphase. Evidently, a strong protective film on sodium is decisive, while the quality of the protective film is mainly determined by its components. However, it is challenging to actively adjust the expected components. This work can regulate the solid electrolyte interphase (SEI) components by introducing a functional electrolyte additive (2-chloro-1,3-dimethylimidazoline hexafluorophosphate (CDIH, namely CDI+ +PF6 - )) into FEC/PC ester-based electrolyte. Specifically, the chloride element in the CDI+ can easily react to form a NaF/NaCl-rich SEI together with the decomposition products of FEC; then the CDI+ without chlorine as a gripper to capture the organic-molecule intermediates generated during FEC decomposition to greatly reduce the content of unstable organic components in SEI, which can be confirmed by molecular dynamic simulation and experiment. Eventually, a highly reversible Na deposition behavior can be delivered. As expected, under the action of CDIH additives, the Na||Na symmetrical cell performs an excellent long-term cycling (>800h, 0.5mA cm-2 -0.5 mAh cm-2 ) and rate performance (0.5-4mA cm-2 ). Furthermore, the Na||PB full cell exhibits the outstanding electrochemical performance with small polarization.

Analysis of seizure-resistance-improvement mechanism of polymer additives by acoustic emission sensing

Analyses of the spectra of acoustic emissions produced by the deformation and fracture of frictional surfaces were used to identify the mechanisms by which polymer additives in lubricating oils improve seizure resistance. Analysis of the frequency spectra of the acoustic signal waveforms permitted the evaluation of interactions between surface asperities that were undetectable from changes in the coefficient of friction. The changes in acoustic emissions explain the action of viscosity modifiers in lubricants in relaxing the contact between surface asperities, and the sensing and analysis of acoustic emissions could provide a new method for analysing the action of lubricant additives.

Probiotic Bacillus Strains Enhance T Cell Responses in Chicken

Banning antibiotic growth promotors and other antimicrobials in poultry production due to the increasing antimicrobial resistance leads to increased feeding of potential alternatives such as probiotics. However, the modes of action of those feed additives are not entirely understood. They could act even with a direct effect on the immune system. A previously established animal-related in vitro system using primary cultured peripheral blood mononuclear cells (PBMCs) was applied to investigate the effects of immune-modulating feed additives. Here, the immunomodulation of different preparations of two probiotic Bacillus strains, B. subtilis DSM 32315 (BS), and B. amyloliquefaciens CECT 5940 (BA) was evaluated. The count of T-helper cells and activated T-helper cells increased after treatment in a ratio of 1:3 (PBMCs: Bacillus) with vital BS (CD4+: p < 0.05; CD4+CD25+: p < 0.01). Furthermore, vital BS enhanced the proliferation and activation of cytotoxic T cells (CD8+: p < 0.05; CD8+CD25+: p < 0.05). Cell-free probiotic culture supernatants of BS increased the count of activated T-helper cells (CD4+CD25+: p < 0.1). UV-inactivated BS increased the proportion of cytotoxic T cells significantly (CD8+: p < 0.01). Our results point towards a possible involvement of secreted factors of BS in T-helper cell activation and proliferation, whereas it stimulates cytotoxic T cells presumably through surface contact. We could not observe any effect on B cells after treatment with different preparations of BS. After treatment with vital BA in a ratio of 1:3 (PBMCs:Bacillus), the count of T-helper cells and activated T-helper cells increased (CD4+: p < 0.01; CD4+CD25+: p < 0.05). Cell-free probiotic culture supernatants of BA as well as UV-inactivated BA had no effect on T cell proliferation and activation. Furthermore, we found no effect of BA preparations on B cells. Overall, we demonstrate that the two different Bacillus strains enhanced T cell activation and proliferation, which points towards an immune-modulating effect of both strains on chicken immune cells in vitro. Therefore, we suggest that administering these probiotics can improve the cellular adaptive immune defense in chickens, thereby enabling the prevention and reduction of antimicrobials in chicken farming.