Estimates of acquiescent response bias derived from previously published, large-scale cross-cultural surveys that used Likert-type response scales are compared. Substantial evidence for convergent validity is found, particularly in relation to the surveys that measured value preferences. High bias in responses to personally relevant items is found in nations that are high on family collectivism and on a preference for increased uncertainty avoidance. High bias in responses to descriptions of others is found in nations low in uncertainty avoidance. These findings suggest that national indicators of acquiescence have substantive cultural meaning and should not be eliminated from nation-level analyses but rather built into analyses of cultural dynamics.