Acoustic Radiation Research Articles

Metabolic-Glycoengineering-Enabled Molecularly Specific Acoustic Tweezing Cytometry for Targeted Mechanical Stimulation of Cell Surface Sialoglycans.

In this study, we developed a novel type of dibenzocyclooctyne (DBCO)-functionalized microbubbles (MBs) and validated their attachment to azide-labelled sialoglycans on human pluripotent stem cells (hPSCs) generated by metabolic glycoengineering (MGE). This enabled the application of mechanical forces to sialoglycans on hPSCs through molecularly specific acoustic tweezing cytometry (mATC), that is, displacing sialoglycan-anchored MBs using ultrasound (US). It was shown that subjected to the acoustic radiation forces of US pulses, sialoglycan-anchored MBs exhibited significantly larger displacements and faster, more complete recovery after each pulse than integrin-anchored MBs, indicating that sialoglycans are more stretchable and elastic than integrins on hPSCs in response to mechanical force. Furthermore, stimulating sialoglycans on hPSCs using mATC reduced stage-specific embryonic antigen-3 (SSEA-3) and GD3 expression but not OCT4 and SOX2 nuclear localization. Conversely, stimulating integrins decreased OCT4 nuclear localization but not SSEA-3 and GD3 expression, suggesting that mechanically stimulating sialoglycans and integrins initiated distinctive mechanoresponses during the early stages of hPSC differentiation. Taken together, these results demonstrated that MGE-enabled mATC uncovered not only different mechanical properties of sialoglycans on hPSCs and integrins but also their different mechanoregulatory impacts on hPSC differentiation, validating MGE-based mATC as a new, powerful tool for investigating the roles of glycans and other cell surface biomolecules in mechanotransduction.

Acoustic radiation from multilayered materials using the reciprocity principle.

Application of the reciprocity principle to evaluate the acoustic radiation from arbitrary multilayered fluid and solid materials is described. To include the effect of shear motion in surrounding media, including viscosity in a fluid, equations for the acoustic radiation from such materials under point force excitation are developed in terms of reflection and transmission coefficients for longitudinal and shear waves. Calculations for forcing on either side of the layered material and in arbitrary directions, and for any asymmetric layer arrangements, are conducted. The frequency range of the calculations is not restricted by thin-plate or thick-plate theory. The test case for the radiation from plates embedded in a viscous and attenuating fluid has been investigated in detail. The effect of viscosity and attenuation has been quantified and shown to be significant at high frequencies. Application to the problem of flow noise is also briefly discussed.

Multi‐Frequency Ultrasound Directed Self‐Assembly

AbstractUltrasound‐directed self‐assembly (DSA) utilizes the acoustic radiation force associated with a standing ultrasound wave field to organize particles dispersed in a fluid medium into specific patterns. State‐of‐the‐art ultrasound DSA methods use single‐frequency ultrasound wave fields, which only allow organizing particles into simple, periodic patterns, or require a large number of ultrasound transducers to assemble complex patterns. In contrast, this work introduces multi‐frequency ultrasound wave fields to organize particles into complex patterns. A method is theoretically derived to determine the operating parameters (frequency, amplitude, phase) of any arrangement of ultrasound transducers, required to assemble spherical particles dispersed in a fluid medium into specific patterns, and experimentally validated for a system with two frequencies. The results show that multi‐frequency compared to single‐frequency ultrasound DSA enables the assembly of complex patterns of particles with substantially fewer ultrasound transducers. Additionally, the method does not incur a penalty in terms of accuracy, and it does not require custom hardware for each different pattern, thus offering reconfigurability, which contrasts, e.g., acoustic holography. Multi‐frequency ultrasound DSA can spur progress in a myriad of engineering applications, including the manufacturing of multi‐functional polymer matrix composite materials that derive their structural, electric, acoustic, or thermal properties from the spatial organization of particles in the matrix.

Three-Dimensional Shear Wave Elastography Using Acoustic Radiation Force and a 2-D Row-Column Addressing (RCA) Array.

Acoustic radiation force (ARF)-based shear wave elastography (SWE) is a clinically available ultrasound imaging mode that noninvasively and quantitatively measures tissue stiffness. Current implementations of ARF-SWE are largely limited to 2-D imaging, which does not provide a robust estimation of heterogeneous tissue mechanical properties. Existing 3-D ARF-SWE solutions that are clinically available are based on wobbler probes, which cannot provide true 3-D shear wave motion detection. Although 3-D ARF-SWE based on 2-D matrix arrays have been previously demonstrated, they do not provide a practical solution because of the need for a high channel-count ultrasound system (e.g., 1024-channel) to provide adequate volume rates and the delicate circuitries (e.g., multiplexers) that are vulnerable to the long-duration "push" pulses. To address these issues, here we propose a new 3-D ARF-SWE method based on the 2-D row-column addressing (RCA) array which has a much lower element count (e.g., 256), provides an ultrafast imaging volume rate (e.g., 2000 Hz), and can withstand the push pulses. In this study, we combined the comb-push shear elastography (CUSE) technique with 2-D RCA for enhanced SWE imaging field-of-view (FOV). In vitro phantom studies demonstrated that the proposed method had robust 3-D SWE performance in both homogenous and inclusion phantoms. An in vivo study on a breast cancer patient showed that the proposed method could reconstruct 3-D elasticity maps of the breast lesion, which was validated using a commercial ultrasound scanner. These results demonstrate strong potential for the proposed method to provide a viable and practical solution for clinical 3-D ARF-SWE.

Bridge-Borne Noise Induced by High-Speed Freight Electric Multiple Units: Characteristics, Mechanisms and Control Measures

The high-speed freight electric multiple unit (EMU) is one of the important development directions for railway freight transportation. To investigate the bridge radiation noise induced by the freight EMU, a noise prediction model consisting of the containers–vehicle–track–bridge dynamic model, finite element model, and boundary element model are established and validated. Through simulation, the bridge radiation noise under different train loading conditions is compared, and the noise radiation mechanism is revealed. Moreover, the noise reduction effect of the noise wall is studied, and the influences of noise wall heights and sound absorption materials are investigated. Results indicate that the bridge sound power and the sound pressure levels (SPLs) of near-field points increase slightly with train loads in the frequency range below 20 Hz and above 125 Hz, with a maximum increase of about 6.8 dB. The structure resonance, intense local vibration, and high acoustic radiation efficiency cause strong bridge radiation noise. The noise wall can realize a good overall noise reduction effect in the sound shadow zone; nevertheless, SPLs increased in areas between the bridge and the noise wall. The ground reflection affects the superposition of transmitted, reflected, and diffracted sound waves, which causes nonlinear relationships of noise reduction effects with the noise wall height. From the perspective of human hearing sensitivity, the loudness levels of typical field points increase with the frequency in the range of 20~80 Hz, and SPLs below 25 Hz are less than the threshold of hearing. Setting the noise wall can effectively reduce the loudness levels, and the reduction effect increases with the noise wall height.

Transdermal drug delivery mediated by acoustic vortex beam

Ultrasound-mediated transdermal drug delivery exhibits various advantages such as biocompatibility, controllability and safety, which attracts plenty of interests within biomedical field. Current researches mostly emphasizes the acoustic cavitation generated by planar or focused waves while neglecting other physics that occur during transportation. Our experimental study illustrates the presence of an acoustic vortex (AV) beam that exhibits a lower acoustic intensity and typically means a lower dose of inertial cavitation, yet achieves a more efficient delivery. Such a result calls for the fundamental mechanism of ultrasound-mediated transdermal transfer using the AV beam. In this work, according to our knowledge, the AV beam is firstly introduced to ultrasound-mediated transdermal medication delivery. The transversal acoustic radiation force (T-ARF), which is the primary characteristic carried by the acoustic vortex beam, and its contribution to the transport enhancement are investigated. It is shown that a focused AV (FAV) beam with a maximal acoustic pressure of 200 kPa induces a pN-level T-ARF, which promotes the enlargement of pores on the stratum corneum and thereby enhances the permeability, as compared with a zero-order (non-vortex) counterpart. This contribution of the T-ARF is validated by the experimental transport on the cellulose membrane, which exhibits a significantly increased membrane porosity and delivery efficiency. The favorable results introduce the new degree of freedom into the ultrasound-mediated transdermal drug transport based on AV beam, and thereby promotes the development of a combined control strategy for more precise and efficient transdermal drug delivery in conjunction with the administration of acoustic cavitation.

Smart Blade Count Selection to Align Modal Propagation Angle with Stator Stagger Angle for Low-Noise Ducted Fan Designs

The rotor–stator interaction noise is a major source of fan noise. Especially for low-speed fan stages, the tonal component is typically a dominant noise source. A challenge is to reduce this tonal noise, as it is typically perceived as unpleasant. Therefore, in this paper, we analytically, numerically and experimentally investigate an acoustic effect to lower the tonal noise excitation. Our study on an existing low-speed fan indicates a reduction in tonal interaction noise of more than 9 dB at the source if the excited acoustic modes propagate parallel to the stator leading edge angle. Moreover, a design-to-low-noise approach is demonstrated in order to apply this effect to two new fan stages with fewer stator than rotor blades. The acoustic design of both fans is determined by an appropriate choice of the rotor and stator blade numbers in order to align the modal propagation angle with the stator stagger angle. The blade geometries are obtained from aerodynamic optimization. Both fans provide similar aerodynamic but opposing acoustic radiation characteristics compared to the baseline fan and a significant tonal noise reduction resulting from the impact of the modal propagation angle on noise excitation. To ensure that this effect can also be applied to other low-speed fans, a design rule is derived and validated.

Acoustic Radiation Forced Impulse of the Liver and the Spleen, Combined with Spleen Dimension and Platelet Count in New Ratio Scores, Identifies High-Risk Esophageal Varices in Well-Compensated Cirrhotic Patients.

Acoustic radiation forced impulse (ARFI) is an integrated ultrasound method, measuring stiffness by point shear wave elastography. To evaluate the diagnostic performance of the ARFI of the liver and the spleen, combined with spleen dimension and platelet count, in predicting high-risk esophageal varices (HRVs) in cirrhotic patients, a prospective and cross-sectional study was conducted between February 2017 and February 2021. The following ratio scores were calculated based on ARFI measurements: ALSDP (ARFI Liver-Spleen Diameter-to-Platelet Ratio Score), ASSDP (ARFI Spleen-Spleen Diameter-to-Platelet Ratio Score), ASSAP (ARFI Spleen-Spleen Area-to-Platelet Ratio Score), and ALSAP (ARFI Liver-Spleen Area-to-Platelet Ratio Score). In 100 enrolled subjects, spleen ARFI, ASSDP, and ASSAP were significantly associated with HRVs in the prospective short- and long-term follow-ups and in the cross-sectional study (p < 0.05), while ALSDP and ALSAP were associated with HRVs only in the prospective long-term follow-up and cross-sectional study (p< 0.05). ASSAP was the best ARFI ratio score for HRVs at the long-term follow-up [value of area under curve (AUC) = 0.88], although all the ARFI ratio scores performed better than individual liver and spleen ARFI (AUC > 0.7). In our study, ARFI ratio scores can predict, in well-compensated cirrhotic patients, the risk of developing HVRs in short- and long-term periods.

A High-Performance Flexible Hydroacoustic Transducer Based on 1-3 PZT-5A/Silicone Rubber Composite.

In recent years, hydroacoustic transducers made of PZT/epoxy composites have been extensively employed in underwater detection, communication, and recognition for their high energy conversion efficiency. Despite the ease with which these transducers can be formed into complex shapes, their lack of mechanical flexibility limits their versatility across various sizes of underwater vehicles. This study introduces a novel flexible piezoelectric composite hydroacoustic transducer (FPCHT) based on a 1-3 PZT-5A/silicone rubber composite and an island-bridge flexible electrode, which can break the limitations of existing hydroacoustic transducers that do not have flexibility. The finite element method is used to optimize the structural parameters of high-performance 1-3 FPC. A large-sized (187 mm × 47 mm × 5.12 mm) FPC is fabricated using an improved cutting-filling method and packaged into the FPCHT. Compared with the planar rigid PZT/epoxy composite hydroacoustic transducer (RPCHT) of the same size, the TVR (186.5 db) of the FPCHT has increased by about 7 dB, indicating that it has better acoustic radiation performance and electroacoustic conversion efficiency. Furthermore, its electroacoustic performance exhibits excellent stability under different bending states. Therefore, the FPCHT with high electroacoustic performance is an ideal substitute for the existing RPCHT and promotes the development of hydroacoustic transducers towards flexibility and portability.

A high-intensity low-frequency acoustic generator based on the Helmholtz resonator and airflow modulator.

The high-intensity low-frequency acoustic sources have essential applications in acoustic biological effects research, airport bird repelling, and boiler ash removal. However, generating high-intensity low-frequency acoustic waves in open space is difficult. In this paper, a low-frequency acoustic generator with a resonant cavity used to enhance the acoustic intensity in open space was developed, which is an aerodynamic acoustic generator to radiates a high-intensity acoustic wave of 52Hz. Some experiments were carried out to measure this generator's internal flow field and radiated acoustic field characteristics, including the propagation characteristics at 100m. The experimental results show that the resonant enhancement effect is presented near the predetermined resonance frequency, and the enhanced value is about 4dB. The acoustic intensity for 52Hz at 1m position is 124dB. By combining the Helmholtz resonator with the airflow modulator, the airflow resonance in the resonator enhances the air pressure pulsation inside the chamber and increases the disturbance of acoustic radiation to the air. So as to improve the sound intensity and radiation efficiency in the low-frequency range.

Extraordinary stability of surfactant‐free bubbles suspended in ultrasound

AbstractGravity‐induced drainage is one of the main destabilizing mechanisms for soap bubbles and foams. Here we show that solely through acoustic levitation without introducing any chemical stabilizers, liquid drainage in the bubble film can be completely inhibited, therefore leading to a significant enhancement of bubble lifetime by more than two orders of magnitude and enabling the bubble to survive puncturing by a needle. Based on sound simulation and force analysis, it has been found that acoustic radiation force, exerted on both the inner and outer surfaces of the levitated bubble, acts in opposite directions, thus providing a squeezing effect to the bubble film. The hydrostatic pressure that induces drainage has been balanced by the acoustic radiation pressure exerted on both sides of the film, which is at the origin of the sound stabilization mechanism. This study provides new insights into the interplay between sound and soap bubbles or films, thus stimulating a wide range of fundamental research concerning bubble films and expanding their applications in bio/chemical reactors.

Vibration and acoustic characteristics of acoustic black hole plates with variable elastic modulus

Acoustic black hole (ABH), as a new wave manipulation technique, shows excellent applications in vibration and noise reduction of structures. Nowadays, most ABHs use materials with a fixed elastic modulus, limiting their low-frequency performance. Herein ABH plates with variable elastic modulus (VM-ABH) is designed, and its vibration and acoustic radiation characteristics are investigated by using numerical analysis. The results show that the vibration response of VM-ABH has a decrease of 5–13.2[Formula: see text]dB relative to that of the uniform texture ABH (UT-ABH) in the frequency range of 10–5000[Formula: see text]Hz, and the degree of energy aggregation is significantly improved. Moreover, the sound pressure level was reduced by 3.6 dB. Meanwhile, by linearly varying the elastic modulus in the center region of the VM-ABH, the effects of gradient index and terminal elastic modulus on the damping characteristics and dynamic response are revealed. The research results provide new objects for the study of vibration and noise reduction of ABH.

Two dimensional shear wave elastography—Basic principles and current applications

AbstractUltrasound‐based elastography techniques are increasingly available on modern ultrasound systems. The application of elastography extends the utility of ultrasound, yielding new information about the impact of disease on tissue biomechanics. By modifying the B‐mode imaging pulse, an Acoustic Radiation Force Impulse produces shear waves in selected tissue. Quantifying the speed of the resultant shear wave provides an indirect assessment of tissue stiffness, with stiffer tissue producing faster shear wave movement than softer tissue. Two main types of ultrasound shear wave elastography are currently in use. Point shear wave elastography uses one or two pulses to measure shear wave speeds over a small fixed area. Alternatively, two‐dimensional shear‐wave elastography provides a colour elastogram map over a larger area, allowing the operator to select specific regions of interest to measure shear wave speed. This article will discuss the essential physics principles, and clinical uses of two‐dimensional shear wave elastography, aiming to provide sonographers with a basic understanding of the technology and current applications.

High-spatial-resolution transcranial focused ultrasound neuromodulation using frequency-modulated pattern interference radiation force

Stimulating the brain in a precise location is crucial in ultrasound neuromodulation. However, improving the resolution proves a challenge owing to the characteristics of transcranial focused ultrasound. In this paper, we present a new neuromodulation system that overcomes the existing limitations based on an acoustic radiation force with a frequency-modulated waveform and standing waves. By using the frequency-modulated pattern interference radiation force (FM-PIRF), the axial spatial resolution can be reduced to a single wavelength level and the target location can be controlled in axial direction electronically. A linear frequency-modulated chirp waveform used in the experiment was designed based on the simulation results. The displacement of the polydimethylsiloxane (PDMS) cantilever was measured at intervals of 0.1 mm to visualize the distribution of radiation force. These results and methods experimentally show that FM-PIRF has improved spatial resolution and capability of electrical movement.

Janus Particles in Acoustofluidic Setup: The Interplay between Self-Propulsion and Acoustic Trapping

Acoustic focusing of particle flow in microfluidics has been shown to be an efficient tool for particle separation for various chemical and biomedical applications. The mechanism behind the method is the selective effect of the acoustic radiation force on distinct particles. In this way, they can be selectively focused and separated. The technique can also be applied under stationary conditions, i.e., in the absence of fluid flows. In this study, the manipulation of self-propelled particles, such as Janus particles, in an acoustofluidic setup was investigated. In experiments with self-propelled Janus particles and passive beads, we explored the interplay between self-propulsion and the acoustic radiation force. Our results demonstrated unusual and potentially useful effects such as selective trapping, escape, and assisted escape in binary mixtures of active and passive particles. We also analyzed various aspects related to the behavior of Janus particles in acoustic traps in the presence and absence of flows.

Particle distributions in Lamb wave based acoustofluidics

Acoustic streaming enabled by a Lamb wave resonator (LWR) is efficient for particle trapping and enrichment in microfluidic channels. However, because Lamb waves combine the features of bulk acoustic waves and surface acoustic waves, the resulting acoustic streaming in the LWR occurs in multiple planes, and the particle flow behavior in this acoustofluidic system is largely unknown. Reported here are numerical simulations and laboratory experiments conducted to investigate the boundary conditions for particle motion inside a microvortex induced by an LWR. Upon dynamic capture, the particles’ trajectories become orbital paths within an acoustic vortex. The suspended particles encounter two distinct acoustic phenomena, i.e., the drag force resulting from acoustic streaming and the acoustic radiation force, which exert forces in various directions on the particles. When the acoustic radiation force and the fluid drag force are dominant for large and small particles in a mixed solution, respectively, the large particles reside within the vortex while the small particles remain at its periphery. Conversely, when the acoustic radiation force is dominant for both types of particles, the distribution pattern is reversed.

Frequency characteristics of an ultrasonic varifocal liquid crystal lens.

Compound lens systems with mechanical actuators are used to focus objects at near to far distances. The focal length of ultrasound varifocal liquid crystal (LC) lenses can be controlled by modulating the refractive index spatial distribution of the medium through the acoustic radiation force, resulting in thin and fast-response varifocal lenses. The frequency characteristics of such a lens are evaluated in this paper, and several axisymmetric resonant vibration modes over 20kHz are observed. The effective lens aperture decreased with the wavelength of the resonant flexural vibration generated on the lens, meaning that this parameter can be controlled with the driving frequency.

Reduced white matter maturation in the central auditory system of children living with HIV.

School-aged children experience crucial developmental changes in white matter (WM) in adolescence. The human immunodeficiency virus (HIV) affects neurodevelopment. Children living with perinatally acquired HIV (CPHIVs) demonstrate hearing and neurocognitive impairments when compared to their uninfected peers (CHUUs), but investigations into the central auditory system (CAS) WM integrity are lacking. The integration of the CAS and other brain areas is facilitated by WM fibers whose integrity may be affected in the presence of HIV, contributing to neurocognitive impairments. We used diffusion tensor imaging (DTI) tractography to map the microstructural integrity of WM between CAS regions, including the lateral lemniscus and acoustic radiation, as well as between CAS regions and non-auditory regions of 11-year-old CPHIVs. We further employed a DTI-based graph theoretical framework to investigate the nodal strength and efficiency of the CAS and other brain regions in the structural brain network of the same population. Finally, we investigated associations between WM microstructural integrity outcomes and neurocognitive outcomes related to auditory and language processing. We hypothesized that compared to the CHUU group, the CPHIV group would have lower microstructural in the CAS and related regions. Our analyses showed higher mean diffusivity (MD), a marker of axonal maturation, in the lateral lemniscus and acoustic radiations, as well as WM between the CAS and non-auditory regions predominantly in frontotemporal areas. Most affected WM connections also showed higher axial and radial diffusivity (AD and RD, respectively). There were no differences in the nodal properties of the CAS regions between groups. The MD of frontotemporal and subcortical WM-connected CAS regions, including the inferior longitudinal fasciculus, inferior fronto-occipital fasciculus, and internal capsule showed negative associations with sequential processing in the CPHIV group but not in the CHUU group. The current results point to reduced axonal maturation in WM, marked by higher MD, AD, and RD, within and from the CAS. Furthermore, alterations in WM integrity were associated with sequential processing, a neurocognitive marker of auditory working memory. Our results provide insights into the microstructural integrity of the CAS and related WM in the presence of HIV and link these alterations to auditory working memory.

3D printed large amplitude torsional microactuators powered by ultrasound

Here, we introduce a design, fabrication, and control methodology for large amplitude torsional microactuators powered by ultrasound. The microactuators are 3D printed from two polymers with drastically different elastic moduli as a monolithic compliant mechanism, and contain precisely engineered capsules with multiple orifices that serve as stators and rotors. Secondary acoustic radiation forces generated among the encapsulated air bubbles controllably rotate the rotor that is supported and stabilized by two torsion bars. The capsules are designed according to the simulations of an analytical model that captures the dynamics of the water-air interface vibrations, which we rigorously validate using a laser Doppler vibrometer and phase-contrast imaging. An in-depth experimental sensitivity analysis is conducted to optimize the arrangement of the rotor and the stators. Integration of experimental results with finite element analysis of the twisting bars and analytical modelling of acoustic phenomena allows us to compute the secondary acoustic radiation forces and the angular displacement of the rotor for a given input pressure. The versatility of the design framework and the robust performance of the printed actuators enable the development of a new class of microscale machines and soft robotic devices that are actuated and controlled by sound waves.

Reflective vortex focusing for acoustic contact-free object rotation

The use of acoustic vortex waves with angular momentum is a promising means of transferring mechanical torque to an object without making any physical contact. However, the existing passive-conversion vortex lenses are unable to produce strong acoustic radiation torque. To address this issue, we propose a flat reflector sculpted by spiral grooves. It reflects and converts incident waves into vortex-focusing waves that generate radiation torque. Compared with a transmission-type lens, a reflection-type vortex focusing plate exhibits superior functionality in converging sound waves and creating a stronger circular intensity flow. In this work, we analyze the vortex-focusing sound by Rayleigh-Sommerfeld integral theory and boundary element simulation, confirmed by experiments. The number, depth and direction of spiral grooves determine the topological charge, focusing intensity and torque direction, respectively. We evaluate the local and total acoustic radiation forces and torques, acting on small and large fixed spherical targets in the vortex-focusing beam. As a result, spinning a centimeter-size origami pinwheel several times larger than the wavelength at 40 kHz is realized without direct contact, by utilizing the enhanced acoustic radiation torque converged by the reflector. This technology enables efficient convergence of acoustic waves and simultaneous contactless transfer of mechanical torque to an object, thereby presenting potential applications in sound energy harvesting and wireless power supplies.