Acne Therapy Research Articles

Endocrine Disorders and Hormonal Therapy for Adolescent Acne

Endocrine Disorders and Hormonal Therapy for Adolescent Acne

Research advances on mesenchymal stem cells and their derivatives in the treatment of acne scars

Acne is a common chronic inflammatory disease of the skin that often occurs on the face, and acne scars are often secondary to the healing process of acne, which often leads to impaired appearance and psychological disorders of patients. The current treatment for acne scars is extremely difficult. With the development of regenerative medicine, stem cell transplantation has become a new treatment for acne scars. In recent years, it has been reported that stem cells and their derivatives can effectively antagonize the formation of acne scars. Therefore, this paper briefly reviews the basic and clinical researches on the treatment of acne scars with various mesenchymal stem cells and their derivatives, aiming to provide theoretical basis and reference for the stem cell therapy of acne scars.

Fibrous palygorskite clays as versatile nanocarriers for skin delivery of tea tree oils in efficient acne therapy

This paper presents a facile approach to develop palygorskite (Pal), a fibrous clay mineral, as a delivery system of tea tree oil (TTO) for topical acne therapy. The obtained TTO-Pal composite showed an efficient loading of TTO (27.4%) with a selective accumulation of terpine-4-ol and 1,8-cineole (two major antimicrobial TTO constituents), sustained release of TTO at skin physiological conditions (pH5.4, 32 °C) and superior skin sebum (2.2 g/g) absorbability. In vitro toxicological assessments showed that the Pal incorporation strategy significantly reduced the acute contact toxicity of TTO. The antimicrobial results revealed a preferable bacteriostatic effect for the TTO-Pal system towards opportunistic dermal pathogens (Escherichia coli, Staphylococcus aureus and Propionibacterium acnes) over the beneficial bacterium (Staphylococcus epidermis). Moreover, TTO-Pal based formulations exhibited pronounced clinical therapeutic efficacy in treating facial acne by rapidly reducing inflamed lesions, modulating skin sebum overproduction and restoring barrier function. This is the first report of using fibrous clay as a biocompatible nanocarrier system for topical delivery of essential oils in efficient management of facial acne with both in vitro and in vivo evidences, which may open perspectives for fibrous clay-drug delivery system in topical application and expand the high added value development of this mineral resource in the advanced healthcare fields.

Risk factors of severe acne vulgaris: Systematic review and meta-analysis

Severe forms of acne vulgaris are accompanied by a pronounced decrease in the quality of life and lead to scarring. Early and effective acne therapy is the prevention of the formation of persistent cosmetic defects.Aim. To identify risk factors and predictors for the development of severe forms of acne vulgaris.Materials and methods. Article searches were performed in PubMed, Google Scholar and the Cochrane Central Registry of Controlled Trials over the period from 15 years to 10/25/2021 for the keywords ‘severe acne’, ‘predictors’ and ‘risk factors’. A meta-analysis was prepared using the Review Manager software (RevMan 5.4.1), The Cochrane Collaboration (2020) in line with international guidelines for systematic reviews and meta-analyzes (PRISMA).Results. 38 publications were included in the review. Risk factors for the development of severe forms of acne vulgaris can be divided into nonmodifable (presence of acne in close relatives, male gender, oily skin type, changes in genes) and modifable (increased BMI, consumption of dairy products, Fast digesting carbohydrates, etc.) In the meta-analysis, a statistically signifcant increase in the risk of severe forms of acne vulgaris in the presence of a history of acne in a close family member was found to be 2.54 times (95 % CI [1.63, 3.91]). It was also found that men have a higher risk of developing severe forms than women (1.16 times {95 % CI [1.04, 1.30]}). People with a BMI higher than 25 (2.54 times {95 % CI [1.63, 3.91]}) and those who consume dairy products 1.59 times more often than three times a week (95 % CI [1.37, 1.85]).Conclusion. Understanding the main risk factors for the development of deep forms of acne vulgaris will help to suspect a severe course of the disease in the early stages and to prescribe effective therapy in time. Male gender, family history, elevated BMI, and dairy consumption suggest a high likelihood of early development of severe acne.

Analysis on the difference of skin surface lipids during blue light therapy for acne by lipidomics.

Acne is a chronic inflammatory skin disease of the sebaceous glands of the hair follicles, caused by a variety of factors and tends to recur, causing skin damage and psychological stress to patients. Blue light (415nm) is a popular physical therapy for acne, however, studies on the effects of blue light on skin surface lipids (SSL) have not been exhaustively reported. So, we want to investigate the difference in SSL before and after acne treatment with blue light and to reveal the potential mechanism of acne treatment with blue light from the lipid level. SSL samples were collected and physiological indicators (moisture content, transepidermal water loss (TEWL), sebum content and pH) were measured. By using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) with multivariate data analysis methods to obtain specific information on the lipid composition. Analysis of the physiological index data showed a significant increase in moisture content (p = 0.042), pH (p = 0.000) and a significant decrease in sebum content(p = 0.008) in the after treatment area (AT group), while there was no significant change in TEWL values. A total of 2398 lipids were detected by lipidomics analysis and 25 differential lipids were screened. Triradylglycerols (TGs), isoprenoids and hopanoids being the potential differential lipids. Among the lipids associated with the skin barrier, only monounsaturated fatty acids (MUFA) (p = 0.045) were significantly increased. This study revealed significant changes in SSL after blue light treatment for acne, suggesting that blue light exposure may cause changes in the relative lipid content and redistribution of lipid components, and that whether it damages the skin barrier requires further study.

The Anti-Inflammatory Effects of Cannabidiol (CBD) on Acne.

Acne is the most common skin condition in the United States and affects approximately 85% of people ages 12–24. As a multifactorial disease, the pathogenesis of acne involves overproduction of sebum, irregular shedding of the cutaneous cells, accretion of Cutibacterium acnes at the pilosebaceous unit, and inflammation. To date, conventional therapies for acne include topical retinoids, over-the-counter bactericidal agents, and systematic treatments, such as oral antibiotics and isotretinoin. However, the potential for significant side effects and risk of antibiotic resistance remain limitations in these therapies, in turn reducing patient compliance and adherence to acne treatment regimens. Therefore, the use of natural plant-derived treatments or phytotherapeutics as an alternative or adjuvant to conventional treatments is attractive to patients due to their safety and minimal risk for side effects. Cannabidiol (CBD) is a non-psychoactive phytocannabinoid of the Cannabis sativa (hemp) plant. The therapeutic use of CBD has been implicated in many diseases with an inflammatory aspect, including cancers, neurodegeneration, immunological disorders, and dermatological diseases. However, the use of CBD for acne treatment remains a novel window of opportunity. Herein, we summarize the available and relevant data, highlighting the potential use of CBD in acne for its anti-inflammatory properties. To that extent, CBD and other cannabis constituents such as cannabis seeds were found to reduce inflammation and expression of inflammatory cytokines including TNF-α and IL-1β when evaluated in acne-like conditions. Treatment with these cannabis extracts was also found to be safe and well tolerated, further strengthening the prospect of CBD as an anti-inflammatory therapeutic for acne.

Skin microbiome reconstruction and lipid metabolism profile alteration reveal the treatment mechanism of Cryptotanshinone in the acne rat

BackgroundAcne has become one of the most prevalent skin disorders, affecting mostly young people's physical and mental health globally. Cryptotanshinone (CPT) is a potential drug for acne, but its mechanism of acne treatment has not been thoroughly studied on the microbiota. Till date, only a few studies are directed to the impact of acne therapy on skin microbiota and lipid metabolites. PurposeThe action mechanism of CPT treatment of acne was investigated by the strategy of microbiome integration with lipidomics. MethodsThe 16Sr DNA sequencing was used to detect skin microbiota composition, and absolute quantitative lipidomics was utilized to identify lipid metabolites profiles levels. Four key proteins of the glycolysis pathway were detected with the immunochemistry method. Antibacterial analysis was used to evaluate CPT treatment of acne. ResultsCPT significantly inhibited Staphylococcus epidermidis and Staphylococcus aureus. Combination of the skin microbiome and lipidomics analysis, 29 types of differentially expressed flora (DEFs) and 782 differentially expressed lipid metabolites (DELMs) were significantly altered, especially Staphylococcus, Corynebacterium, Ralstonia, Enhydrobacter, Burkholderia, and Streptococcus. Cer was mainly regulated by Staphylococcus and Corynebacterium, whereas TG and DG were mainly regulated by Ralstonia, Enhydrobacter, Burkholderia, and Streptococcus. The glycolysis pathway was significantly regulated by Staphylococcus on CPT treatment of acne. The energy metabolism, lipid metabolism, immune system, glycan biosynthesis, and metabolism could be reversed by CPT. ConclusionCPT might help acne rats rebuild their skin microbiota and alter lipid metabolism signatures. Furthermore, since skin microbes and skin lipid metabolites have a close correlation and are both regulated by CPT, the findings potentially provide a research foundation for the discovery of biomarkers of skin microbiome imbalance and targeted treatment of acne development mechanisms.

Topical dapsone in the treatment of acne: a systematic review.

Acne is one of the most common dermatological disorders. Initial therapies for acne include topical retinoids, benzoyl peroxide, and topical clindamycin. However, patients who fail initial therapies may benefit from alternative topicals, including dapsone gel. To analyze the current literature studying the efficacy of topical dapsone in the treatment of acne. PubMed, Embase, and Cochrane Library were systematically searched for clinical trials examining the efficacy of topical dapsone in the treatment of acne. Fourteen studies were included in the analysis. Dapsone monotherapy showed a treatment success rate of 40.1-69.4% for dapsone gel 5% and 29.8-47.0% for dapsone gel 7.5% when used for 12-16 weeks. In all studies, inflammatory lesions decreased by a larger percentage than noninflammatory or total lesions. Dapsone gel was also studied in combination with various other acne treatments, including doxycycline, oral isotretinoin, benzoyl peroxide, and topical retinoids. While mild treatment-related adverse effects, most commonly consisting of skin irritation, occurred in 2.0-75.0% of participants, no major treatment-related adverse effects were reported. Limitations of the study include variable treatment regimens making it difficult to compare results across studies. Additionally, adverse effects and skin irritation were reported differently, and potential selection biases exist in the randomized trials. Dapsone gel offers a safe and promising alternative therapy for patients with difficult to treat acne or those who experience adverse effects to first-line therapies.

New Developments in Topical Acne Therapy.

Acne vulgaris is a common chronic inflammatory disease with a multifactorial pathogenesis. Although myriad acne treatments are available, current options may not be sufficient because of a lack of efficacy, limited tolerability, or burden of cost to patients. In this review, we highlight recently approved topical acne treatments, as well as those currently in clinical trials. Novel formulations of tretinoin, tazarotene, and minocycline provide modifications of and improvements to existing products. Trifarotene, a novel fourth-generation retinoid, has demonstrated improved tolerability compared with existing topical retinoids. Clascoterone is a novel first-in-class antiandrogen that topically addresses the hormonal etiology of acne. The late-phase clinical trials pipeline consists of agents with bactericidal and anti-sebum mechanisms. Although it is evident that acne treatments continue to evolve, it is important to recognize the need for further comparative studies among new and existing agents to define optimal treatment algorithms that address not only safety and efficacy but also cost-effective care.

Insights from a Box-Behnken Optimization Study of Microemulsions with Salicylic Acid for Acne Therapy.

The present study brings to attention a method to develop salicylic acid-based oil in water (O/W) microemulsions using a tensioactive system based on Tween 80, lecithin, and propylene glycol (PG), enriched with a vegetable oat oil phase and hyaluronic acid. The systems were physically characterized and the Quality by design approach was applied to optimize the attributes of microemulsions using Box–Behnken modeling, combined with response surface methodology. For this purpose, a 33 fractional factorial design was selected. The effect of independent variables namely X1: Tween 80/PG (%), X2: Lecithin (%), X3: Oil phase (%) was analyzed considering their impact upon the internal structure and evaluated parameters chosen as dependent factors: viscosity, mean droplet size, and work of adhesion. A high viscosity, a low droplet size, an adequate wettability—with a reduced mechanical work—and clarity were considered as desirable for the optimal systems. It was found that the optimal microemulsion which complied with the established conditions was based on: Tween 80/PG 40%, lecithin 0.3%, oat oil 2%, salicylic acid 0.5%, hyaluronic acid 1%, and water 56.2%. The response surface methodology was considered an appropriate tool to explain the impact of formulation factors on the physical properties of microemulsions, offering a complex pattern in the assessment of stability and quality attributes for the optimized formulation.

Progress of different treatment modalities to limit the use of antibiotics in the treatment of acne

Acne vulgaris is one of the most common skin diseases, affecting mainly teenagers. Its treatment procedure is complex, with a long duration of medication. Antibiotics are the most preferably prescribed drugs for the treatment of acne. The long-term use of antibiotics leads to various adverse effects such as the disruption of indigenous flora and resistance. Therefore, numerous therapeutic protocols such as antimicrobial stewardship have been proposed to limit the haphazard use of oral antibiotics. This review emphasizes different topical drugs, systemic alternative drugs, laser, and light therapy, as effective therapies for acne. This review also briefly reflects the efficacy of fire needle therapy—a traditional Chinese therapy—for acne.

Current causes of isotretinoin ineffectiveness in acne therapy

<h3></h3> Акне является заболеванием, характеризующимся избыточной продукцией кожного сала, фолликулярным гиперкератозом, размножением <i>Cutibacterium acnes</i> и воспалением. Системный ретиноид изотретиноин (13-цис-ретиноевая кислота), предназначенный для терапии узловато-кистозных форм угрей, стал препаратом выбора при лечении среднетяжелых и тяжелых форм акне и широко используется в клинической практике. Особое внимание уделяется исследованиям, направленным на разработку оптимальных схем применения данного лекарственного средства. Однако в ряде случаев наблюдаются невосприимчивость пациентов к его применению или развитие рецидивов акне после первого курса лечения. Факторами риска повторного возникновения акне после проведенного курса терапии изотретиноином могут являться тяжелая форма заболевания, мужской пол, неадекватная кумулятивная доза или недостаточная продолжительность терапии, молодой возраст пациентов, а также отягощенный по акне семейный анамнез. Важным направлением в повышении эффективности лечения и выявлении пациентов с резистентными формами акне является детализация вероятных причин неэффективности изотретиноина с целью персонификации курсового режима и уточнения терапевтической тактики применения препарата.

The use of isotretinoin in acne therapy in early childhood and its effect on the occurrence of acne symptoms later in life. Eight-year follow-up.

IntroductionAcne vulgaris is a chronic inflammatory skin disease of the pilosebaceous follicles that affects patients of all ages.AimUse of isotretinoin in the early stages of the disease to prevent subsequent lesions of acne, including prolonged treatment and acne scars at a later age.Material and methodsA retrospective, comparative study was carried between January 2010 and November 2018. The study population consisted of 90 children aged 9–18 years with acne. During treatment by isotretinoin the clinical evaluation was done every month. Patients were divided into three groups according to age. One of the qualification criteria was follow-up visits.ResultsA total of 90 children (67.8% females; mean age: 13.5 years) were enrolled. In group A (30 individuals – aged 9–11) and B (30 individuals – aged 12–13), treatment was terminated 2 months after clinical improvement (mean: 3 months). In control group C (30 individuals – aged 14–18), treatment was carried out using average cumulative dose 135 mg/kg bw/day. All groups showed up for follow-up. after 1 to 8 years. In groups A and B, 13 people underwent a second acne treatment; in 3.33% oral isotretinoin was used, in 18.33% topical treatment. In group C, 30 (100%) individuals underwent a second acne treatment; in 20% oral isotretinoin was used, and 80% required a topical treatment. Acne scars and post acne hyperpigmentation have been documented in 73.33% in group C.ConclusionsEarly, reasonable and short-term use of isotretinoin can reduce the incidence of acne in the future and reduce the occurrence of secondary acne symptoms.

Design and realization of a dual-wavelength low level light therapy for acne and face rejuvenation treatment

Low-level light therapy (LLLT) uses the light of wavelength between 400–700 nm to treat acne, reduce inflammation, stimulate collagen production, and rejuvenate the facial skin. This study designed and constructed a dual-wavelength LED LLLT device for the facial treatment. The light spectrum, power density, uniformity, stability, and safety of the device were analyzed. The proposed system consisted of an LED array with 415 and 633 nm wavelengths. Human machine interface with embedded system was used to control light intensity and treatment time. The phototherapy device is designed to be curvaceously sized to suit the face shape of Asian people. The results showed that the LLLT device emitted 633±5 nm red and 415±5 nm blue light with a linear adjustable light power density of 0-18.56 mW/cm&lt;sup&gt;2&lt;/sup&gt; and 0-3.70 mW/cm&lt;sup&gt;2&lt;/sup&gt;, respectively. The spectrum distribution of the red and blue light was relatively constant over 30 minutes of operation. The uniformity and stability of red spectrum were about 89.9% and 95.08% and blue spectrum were 87.6% and 97.08%, respectively. The experimental face’s temperature was below 31.5 . For the future study, the LED phototherapy device will be applied for clinical research in collaboration with dermatologists.Low-level light therapy (LLLT) uses the light of wavelength between 400–700 nm to treat acne, reduce inflammation, stimulate collagen production, and rejuvenate the facial skin. This study designed and constructed a dual-wavelength LED LLLT device for the facial treatment. The light spectrum, power density, uniformity, stability, and safety of the device were analyzed. The proposed system consisted of an LED array with 415 and 633 nm wavelengths. Human machine interface with embedded system was used to control light intensity and treatment time. The phototherapy device is designed to be curvaceously sized to suit the face shape of Asian people. The results showed that the LLLT device emitted 633±5 nm red and 415±5 nm blue light with a linear adjustable light power density of 0-18.56 mW/cm&lt;sup&gt;2&lt;/sup&gt; and 0-3.70 mW/cm&lt;sup&gt;2&lt;/sup&gt;, respectively. The spectrum distribution of the red and blue light was relatively constant over 30 minutes of operation. The uniformity and stability of red spectrum were about 89.9% and 95.08% and blue spectrum were 87.6% and 97.08%, respectively. The experimental face’s temperature was below 31.5 . For the future study, the LED phototherapy device will be applied for clinical research in collaboration with dermatologists.

Efficacy and tolerability of system isotretinoin and effect of this therapy on the quality of life of patients with severe and moderate acne

Background. The relationship between acne and depression is being actively studied by the medical community. Question is depression a restriction in prescribing or a side effect when using systemic isotretinoin remains controversial. Noteworthy is the presence in the scientific literature of data on the possible positive effect of adequately and timely prescribed acne therapy on the psychoemotional state of patients with this ailment.&#x0D; Aims. The aim of our study was to evaluate the effectiveness of therapy with systemic isotretinoin in patients with moderate and severe acne, to assess the effect of the therapy on the quality of life of patients, as well as the persistence of remission after the end of the course of therapy with systemic retinoid.&#x0D; Methods. We observed 32 patients with moderate and severe acne, the average age of patients was 24 years, the duration of the disease was on average 8 years. All patients underwent therapy with systemic isotretinoin (Sotret) at an average daily dose of 0.51.0 mg/kg until a cumulative dose of 120150 mg/kg was reached. The effectiveness and tolerability of the therapy was assessed, as well as the quality of life of patients was assessed using the dermatological index of the quality of life (DQL), the dermatological akne index (DIA), the HADS scale (anxiety and depression scale). The indicators were assessed before the start of therapy, during therapy, at the end of the course of treatment with systemic isotretinoin, and also 12 months after the end of therapy.&#x0D; Results. 100% of patients achieved clinical remission as a result of treatment with systemic isotretinoin.&#x0D; Before starting therapy with systemic isotretinoin, the indices were as follows: DQL 18 (1520), HADS 10 (716) and DIA 13 (615) scores. By the end of the therapy, the indices decreased to 1 (01), 2 (04) and 1 (01) points, respectively (p 0.001).&#x0D; 12 months (year) after the end of therapy with systemic isotretinoin, the indices remained at zero or one level in all patients: DQL 1 (01), HADS 0 (01) and CIA 1 (01) points (p 0.001).&#x0D; Conclusion. A causal relationship between the intake of systemic isotretinoin and the development of depression has not been established. Systemic isotretinoin therapy was effective in all patients (100%), was well tolerated and had a positive effect on the psychoemotional status of patients with acne. 12 months after the end of therapy with the drug Erase, stable remission of the disease was noted in all patients

Sensitivity of Propionibacterium acnes towards Commercial Anti-Acne Formulations

Propionibacterium acnes are aerotolerant anaerobic, gram-positive bacilli that form part of normal flora. They produce several pro-inflammatory substances that can trigger an immune response in the host by an influx of inflammatory leukocytes into the strands, causing inflammatory lesions that leave behind scars. Repeated isolation of Propionibacterium acnes may reduce efficacy among the resistant types, clearly explaining Acne lesions' importance. The Counter acne therapies are often the first treatment choice due to the convenience of cost and time over clinical appointments. However, not all of the commercially available anti-acne formulations are supported by clinical studies. The present study was conducted to test the efficacy of selected commercial anti-acne gel formulations. The microscopic observation and biochemical studies conform to the presence of anti-acne activity. A sensitivity test was performed on all the isolates of Propionibacterium acnes by well diffusion technique. The selected over-the-counter anti-acne gel formulations failed to produce any inhibition zone.

Molecule which changed the view on skin treatment

Objective — to study the modern classification, mechanisms of action and clinical effects of vitamin A derivatives, to analyze retinoid for local treatment of various dermatoses with a universal mechanism of action at the epidermis and dermis levels.&#x0D; Materials and methods. A review of the literature and an analysis of the results of international clinical trials of drugs based on the natural retinoid of the first generation — tretinoin (retinoic acid) is presented. The works of dozens of authors since 1980s to the present day are analyzed. Most sources provide detailed information on the results of topical retinoids in acne therapy, which are the base of clinical guidelines. Long-term (6 months or more) studies of retinoic acid-based preparations carried out in recent decades have discovered the unique clinical effects of tretinoin in the treatment of skin photoaging, actinic keratosis, etc. They are achieved due to the effect of tretinoid on the nuclear receptors of keratinocytes and fibroblasts.&#x0D; Results and discussion. The molecular mechanisms of action of retinoic acid, realizing the cellular and tissue effects of the most studied retinoid, are systematized and grouped in a single review. It has been proven that a unique feature of tretinin is its ability to activate directly all subtypes of RARs- and, indirectly, RARs-nuclear receptors of skin cells. A new modern drug for external use is presented — AltrenoТМ lotion containing micronized 0.05 % tretinoin in combination with sodium hyaluronate, soluble collagen and glycerin. This combination exhibits the expected clinical efficacy in acne therapy and prevents side effects such as dryness, redness and exfoliation. AltrenoТМ is approved for use in children of 9 years of age and older.&#x0D; Conclusions. Tretinoin (retinoic acid) is a modern powerful retinoid with a universal mechanism of action, recommended for the treatment of acne.

Acne therapy with lasers and light including photodynamic therapy

AbstractUtilizing lasers and light sources for the treatment of acne vulgaris has gained in popularity over the past several years. Newer and safer devices have been introduced which have been shown to be beneficial for many suffering from acne vulgaris. This manuscript will review some of the most important lasers and light sources for acne vulgaris, from the beginning of our understanding of these to current day uses.

Azelaic acid and Melaleuca alternifolia essential oil co-loaded vesicular carrier for combinational therapy of acne.

Aim: Azelaic acid (AzA), a comedolytic, antibacterial, anti-inflammatory anti-melanogenic agent, prescribed against acne vulgaris is safe on skin. Its combination with another widely used anti-acne agent, tea tree oil (EO) whose delivery is limited by volatility, instability and lipophilicity constraints was attempted. Method: Solvent injection was used to prepare AzA-EO integrated ethosomes. Result: Ethosomes were transformed into carbopol hydrogel, which exhibited pseudo-plastic properties with appreciable firmness, work of shear, stickiness and work of adhesion. The hydrogel showed better permeation and retention characteristics vis-a-vis commercial formulation (AzidermTM), when evaluated in Wistar rat skin. Further, ethosome hydrogel composite was better tolerated with no side effects. Conclusion: The findings suggests that the aforementioned strategy could be a potential treatment used for acne management.

The prospects of finding new treatments for acne

An important role in the development of acne is played by the activity of the skin microflora (namely the bacteria Propionibacterum acnes) and members of the genus Staphylococcus. In the schemes of complex treatment of patients with this disease, antibiotics from among macrolides or lincosamides are mainly used. It is of interest to study the current state of susceptibility of staphylococci and propionic bacteria to these drugs, as well as to other antimicrobial agents in order to determine the prospects for expanding the arsenal of methods for treating acne. The aim of the study was to study the susceptibility of microorganisms involved in the development of acne to antimicrobials and antagonistic effects of probiotic strains of lactobacilli. We conducted a bacteriological study of the content of rash elements in 60 patients with acne. The biological characteristics of 40 strains of Staphylococcus spp. and 15 strains of Propionibacterum acnes isolated and researched. The sensitivity of microorganisms to antibiotics and antiseptics was determined by the disco-diffusion method and the method of serial double dilutions of drugs in a liquid nutrient medium. Determination of the sensitivity of acne pathogens to the antagonistic effect of lactobacilli was performed by the method of two-layer agar by reducing the number of microorganisms. The results of the study made it possible to establish that the isolated strains of bacteria show a low level of sensitivity to antimicrobials, which in medical practice are most often used for topical therapy of acne. Only 17.5 % of isolated staphylococcal strains were sensitive to erythromycin, only 45.0 % of strains of this species were sensitive to clindamycin. Strains of Propionibacterum acnes showed a higher level of sensitivity to antibiotics than staphylococci. However, the established indicators do not allow to consider highly effective means for treatment of acne on their basis. At the same time, a high level of sensitivity of acne pathogens to antiseptic drugs decamethoxine and Aeroplysinin 1 and antagonistic effect of Lactobacillus acidophilus was established. Thus, the strains of Propionibacterum acnes and Staphylococcus spp., involved in the development of acne, are characterized by high levels of resistance to erythromycin, clindamycin, chloramphenicol, benzoyl peroxide, which are now the basis of the arsenal of topical therapy for patients with this disease. There is a need to find new effective drugs for the etiotropic therapy of acne.