Acid In Guinea Pigs Research Articles

Studying immunotherapeutic properties of the conjugate based on BCG antigens with betulonic acid in guinea pigs infected with <i>Mycobacterium scrofulaceum</i>

Studying immunotherapeutic properties of the conjugate based on BCG antigens with betulonic acid in guinea pigs infected with <i>Mycobacterium scrofulaceum</i>

Evaluation of anti-inflammatory effect of omega 3 polyunsaturated fatty acid in Guinea pigs

: We need drugs to decrease inflammation as well as have very less side effects. Review of literature mentioned anti-inflammatory action of Omega 3 fatty acids. Hence, we selected this drug. Our aim was to find the anti-inflammatory effect of Omega 3 fatty acid using animal model.: The study is objective animal study where measurement of inflammation was done by observational methodology, for the present study four groups were made- 6 Guinea pigs in each group. Control guinea pigs were fed distilled water, second group standard drug indomethacin 4mg/ kg body wt, test group 1 received 1200mg/kg body wt, test group II received 2400mg/kg body wt of Omega 3 fatty acid. All drugs given orally 30 minutes before exposure to UV radiation of 30 seconds duration was given and inflammation was observed at 6, 12 and 24 hrs after UV exposure in the form of scoring.Statistical significance was calculated using Anova method. For statistical analysis we have used SPSS system version 20. Inflammation was observed in all the four groups of Guinea pigs, there was significant anti-inflammatory effect of omega 3 fatty acid in the dose of 1200mg/kg body wt and 2400mg/kg body wt at 12 and 24 hours. Omega 3 fatty acids 1200mg/kg body wt orally produced significant anti-inflammatory effect against UV radiation induced inflammation in Guinea pigs at 12 and 24 hrs. However, standard drug could produce significant anti-inflammatory effect only at 24 hrs in comparison to control group. The anti-inflammatory effects of test 1, test 2 groups and standard group are comparable.

Aerosolization Performance, Antitussive Effect and Local Toxicity of Naringenin-Hydroxypropyl-β-Cyclodextrin Inhalation Solution for Pulmonary Delivery.

The aim of present study was to evaluate the feasibility of a naringenin-hydroxypropyl-β-cyclodextrin (naringenin-HPβCD) inhalation solution for pulmonary delivery. Naringenin, a flavanone derived from citrus fruits, has been proven to exhibit excellent peripheral antitussive effect. To address the limitation of its poor oral bioavailability and low local concentration in the lung, a naringenin-HPβCD inhalation solution was prepared for pulmonary delivery. The aerosolization performance of formulation was evaluated by next generation impactor (NGI). Both dose-dependent and time-dependent antitussive effects of naringenin-HPβCD inhalation solution on acute cough induced by citric acid in guinea pigs were investigated. In vitro toxicity of naringenin-HPβCD inhalation solution in pulmonary Calu-3 cells was evaluated by MTS assay, and in vivo local toxicity investigation was achieved by assessing bronchoalveolar lavage (BALF) and lung histology after a 7-day inhalation treatment in guinea pigs. Fine particle fraction (FPF) of the formulation was determined as 53.09%. After inhalation treatment of 15min, naringenin-HPβCD inhalation solution within the studied range of 0.2-3.6mg/kg could dose-dependently reduce the cough frequency with the antitussive rate of 29.42-39.42%. Naringenin-HPβCD inhalation solution in concentration range of 100-400μM did not decrease cell viability of Calu-3 cells, and the maximum effective dose (3.6mg/kg) was non-toxic during the short-term inhalation treatment for guinea pigs. In conclusion, naringenin-HPβCD inhalation solution was capable for nebulization and could provide rapid response with reduced dose for the treatment of cough.

A Physicochemical Assessment of Acid-induced Post-inhalation Cough in a Guinea Pig Animal Model

A study was conducted with a Guinea Pig (GP) cough model to demonstrate that post-inhalation (PI) cough can be correlated and predicted with the acidity constant of the chemical stimulant. The test animals were exposed to nebulized solutions of citric acid and maleic acid. Cough responses were measured by audio, video, and respiratory flow signals via whole-body plethysmography. Nonparametric one-way ANOVA (Kruskal-Wallis) of cough counts showed statistically significant dose dependence for both citric acid (p<0.0001) and maleic acid (p<0.0001). For citric acid, post-hoc Dunn’s test indicated elevated cough response at the higher concentrations (p ≤ 0.0001 for 200 mM, and 300 mM) compared to control, i.e., isotonic saline (SL). The cough count at 100 mM citric acid was not significantly different from SL (p>0.05). Similar results were obtained for maleic acid exposures, with cough response at the higher concentrations significantly different compared to SL (p=0.0039 for 50 mM, p<0.0001 for 100 mM), while cough counts at 30 mM were not different compared to SL. The GP model is able to differentiate the cough response of maleic acid vs. citric acid, and the threshold of cough (1-2 coughs in 2 min) for maleic acid (~30 mM) is lower than that for citric acid (~100 mM). The lower threshold of cough for maleic acid as compared to citric acid is consistent with the mechanism of action mediated by protons, in a dose dependent way. A prediction of the cough response from citric acid to maleic acid was confirmed by this study. Experiments with nebulized acids confirmed expectations that the threshold of cough for citric acid in GP is at 100 mM and for maleic acid at 10- 30 mM. The lower cough threshold for maleic vs. citric acid is consistent with proton-mediated mechanism of action.

Reproductive performance and gestational effort in relation to dietary fatty acids in guinea pigs

BackgroundDietary saturated (SFAs) and polyunsaturated (PUFAs) fatty acids can highly affect reproductive functions by providing additional energy, modulating the biochemical properties of tissues, and hormone secretions. In precocial mammals such as domestic guinea pigs the offspring is born highly developed. Gestation might be the most critical reproductive period in this species and dietary fatty acids may profoundly influence the gestational effort. We therefore determined the hormonal status at conception, the reproductive success, and body mass changes during gestation in guinea pigs maintained on diets high in PUFAs or SFAs, or a control diet.ResultsThe diets significantly affected the females’ plasma fatty acid status at conception, while cortisol and estrogen levels did not differ among groups. SFA females exhibited a significantly lower body mass and litter size, while the individual birth mass of pups did not differ among groups and a general higher pup mortality rate in larger litters was diminished by PUFAs and SFAs. The gestational effort, determined by a mother’s body mass gain during gestation, increased with total litter mass, whereas this increase was lowest in SFA and highest in PUFA individuals. The mother’s body mass after parturition did not differ among groups and was positively affected by the total litter mass in PUFA females.ConclusionsWhile SFAs reduce the litter size, but also the gestational effort as a consequence, PUFA supplementation may contribute to an adjustment of energy accumulations to the total litter mass, which may both favor a mother’s body condition at parturition and perhaps increase the offspring survival at birth.

Morphometric evaluation of wound healing in burns treated with Ulmo (Eucryphia cordifolia) honey alone and supplemented with ascorbic acid in guinea pig (Cavia porcellus).

BackgroundIn the context of the search for cost-efficient treatments, Ulmo (Eurcyphia cordifolia) honey is an excellent alternative for treating burn wounds and could have a profound medical, social, and economic impact. Ascorbic acid is an enzymatic co-factor necessary for the synthesis of collagen and the proliferation of fibroblasts and has been proposed as a coadjuvant to strengthen the healing effects of honey. The aim of this work was to evaluate by morphometric studies the healing wounds caused by burns treated with Ulmo honey alone and supplemented with ascorbic acid in guinea pig (Cavia porcellus).MethodsFifteen guinea pigs were used and divided randomly into three groups: positive control (C+), experimental with unsupplemented honey (H), and experimental with supplemented honey (SH). A uniform deep burn covering 1 cm2 of the back skin was performed. The following indices were calculated for the morphometric study: superficial contraction index of the wound, deep contraction index of the wound, wound severity index, global healing index, global contraction index, and dermal proliferation area.ResultsThe superficial contraction index of the wound, global healing index, global contraction, and dermal proliferation area values of the experimental with supplemented honey group were higher than the other groups (P < 0.05).ConclusionsAccording to these results, the combination of honey with an antioxidant (ascorbic acid) promotes an appropriate action to support the healing effect. This study showed that by supplementing the Ulmo honey with ascorbic acid, the healing and contraction effects can be strengthened in burn wounds compared to unsupplemented honey. These results were proof of the synergy between honey and ascorbic acid in healing burn wounds.

Sa1701 Profiles of Gut Microbiota and Production of Short Chain Fatty Acids in Guinea Pigs

Sa1701 Profiles of Gut Microbiota and Production of Short Chain Fatty Acids in Guinea Pigs

Antitussive and expectorant activities of Potentilla anserina

Context The root of Potentilla anserina L. (Rosaceae) is an herbal medicine that has been used as an antitussive and expectorant drug for thousands of years in Chinese folk medicine.Objective: This study estimated the antitussive and expectorant effects of P. anserina extract to validate its traditional use.Materials and methods The antitussive and expectorant activities of the ethanol extract, aqueous extract, and polysaccharides from P. anserina were evaluated using classical animal models.Results The results showed that in three antitussive tests, the aqueous extract and polysaccharides at high and low doses significantly inhibited the frequency of cough induced by ammonia and sulfur dioxide in mice and by citric acid in guinea pigs, and increased the latent period of cough in guinea pigs. Similarly, the aqueous extract and polysaccharides also showed significant expectorant activity compared with the control in phenol red secretion experiments. Polysaccharides at dose of 600 mg/kg enhanced tracheal phenol red output by 121.1%, the ammonium chloride (positive control) at dose of 1000 mg/kg by 117.4%. However, the ethanol extract at a high dose (600 mg/kg) has antitussive activity only in the sulfur dioxide induced coughing test. Moreover, the polysaccharides at the same dose showed better bioactivity than the aqueous extract in all tests.Discussion and conclusion The results of the present study provide evidence that P. anserina can be used as an antitussive and expectorant herbal medicine and that polysaccharides may be the main active ingredients of P. anserina responsible for its bioactivities.

Impaired oxidoreduction by 11β-hydroxysteroid dehydrogenase 1 results in the accumulation of 7-oxolithocholic acid

11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) mediates glucocorticoid activation and is currently considered as therapeutic target to treat metabolic diseases; however, biomarkers to assess its activity in vivo are still lacking. Recent in vitro experiments suggested that human 11β-HSD1 metabolizes the secondary bile acid 7-oxolithocholic acid (7-oxoLCA) to chenodeoxycholic acid (CDCA) and minor amounts of ursodeoxycholic acid (UDCA). Here, we provide evidence from in vitro and in vivo studies for a major role of 11β-HSD1 in the oxidoreduction of 7-oxoLCA and compare its level and metabolism in several species. Hepatic microsomes from liver-specific 11β-HSD1-deficient mice were devoid of 7-oxoLCA oxidoreductase activity. Importantly, circulating and intrahepatic levels of 7-oxoLCA and its taurine conjugate were significantly elevated in mouse models of 11β-HSD1 deficiency. Moreover, comparative enzymology of 11β-HSD1-dependent oxidoreduction of 7-oxoLCA revealed that the guinea-pig enzyme is devoid of 7-oxoLCA oxidoreductase activity. Unlike in other species, 7-oxoLCA and its glycine conjugate are major bile acids in guinea-pigs. In conclusion, the oxidoreduction of 7-oxoLCA and its conjugated metabolites are catalyzed by 11β-HSD1, and the lack of this activity leads to the accumulation of these bile acids in guinea-pigs and 11β-HSD1-deficient mice. Thus, 7-oxoLCA and its conjugates may serve as biomarkers of impaired 11β-HSD1 activity.

Melitidin: A Flavanone Glycoside from Citrus grandis ‘Tomentosa’

Citrus grandis 'Tomentosa' is a traditional Chinese medicine, used as an antitussive. In this research, melitidin, a flavanone glycoside, was isolated from this species for the first time by using chromatographic methods. The structure was confirmed through comprehensive analyses of its ultraviolet, infrared, 1H and 13C NMR, HMBC and HMQC spectroscopic and high-resolution mass spectrometric data. Meliditin showed a good antitussive effect on cough induced by citric acid in Guinea pig, suggesting that it was a contributor to the antitussive effect of C. grandis 'Tomentosa'.

Effects of Middle Ear Packing with Collagen Polyvinylpyrrolidone and Hyaluronic Acid in Guinea Pigs

ABSTRACTObjective: The aim of this study was to evaluate otoscopic and microscopic changes produced on the healthy mucosa of the middle ear (ME) and tympanic membrane (TM) of guinea pigs after packing with a collagen polyvinylpyrrolidone (CPVP) sponge soaked in hyaluronic acid (HA). Material and methods: In 24 guinea pigs, myringotomy on the right side was created and the ME was packed as follows: Group I (n = 6): Absorbable gelatin sponge (AGS) soaked in saline solution; Group II (n = 6): AGS sponge soaked in HA, Group III (n = 6): CPVP sponge soaked in saline solution, Group IV (n = 6): CPVP sponge soaked in HA. Four weeks after miringotomy, the ME and TM integrity and residual packing material were evaluated otoscopically. Histologically, we evaluated inflammatory changes on the ME mucosa. Results: All animals in Groups I and II showed residual packing material (p < .001 ANOVA, TUKEY). Histologically, more inflammation was observed in Groups I, II, and III than in Group IV (p < .001 ANOVA, TUKEY). Group IV showed greater fibroblastic reaction (p < .02, ANOVA, TUKEY) versus other groups. Conclusion: The CPVP sponge soaked in HA used as ME packing material is biocompatible and nontoxic, because it produces minimal inflammatory changes on the healthy mucosa of the ME and TM of guinea pigs. However, more research with injured mucosa is needed to validate its usefulness in otosurgery.

Anti-Obesity Effects of Chitosan and Psyllium Husk with L-Ascorbic acid in Guinea Pigs

The aim of this study was to investigate whether L-Ascorbic acid would facilitate the anti-obesity effects of chitosan and psyllium husk in vivo. The study was carried out with male Hartley guinea pigs for 5 weeks. The results show that chitosan itself did not influence body weight gain and food efficiency ratio (FER). However, the addition of L-Ascorbic acid to chitosan decreased these parameters; the body weight gain and FER in the chitosan-2 group (high-fat diet group with 5 % chitosan containing 0.5 % L-Ascorbic acid) was significantly (p < 0.05) lower than that in F-controls (high-fat diet group), and was similar to that in controls (normal diet group). L-Ascorbic acid enhanced significantly (p < 0.05) the increases of total fecal weight and fecal fat excretion by chitosan. The addition of L-Ascorbic acid to psyllium husk did not differ from psyllium husk alone in terms of changes in weight gain, plasma lipid levels, and fat pad weight. We found that the addition of L-Ascorbic acid to chitosan influenced the reduction in body weight gain and FER, and the increase in total fecal weight and fecal fat excretion in guinea pigs fed a high-fat diet.

Antitussive Activity of Extracts from Fallopia Sachalinensis

Antitussive Activity of Extracts from Fallopia Sachalinensis Giant knotweed (Fallopia sachalinensis) and related plants are commonly used in Chinese and Japanese folk medicine. Bioactive natural products derived from them are believed to possess a variety of biologic activities. We have analyzed two polysaccharide fractions from leaves of Fallopia sachalinensis. We have focused on its activities to experimentally induced cough reflex and the changes of specific airway resistance. We have compared the observed antitussive activity with cough suppressing activity of codeine ("positive" control) and effect acquired after application of water for injection ("negative" control) using conscious male guinea pigs individually placed in a double chambers bodyplethysmograph box. Peroral administration of both polysaccharides significantly inhibited the number of coughs induced by citric acid in guinea pigs and have not significantly changed the values of specific airway resistance. Moreover, the substances in experimental animals have not provoked any notable adverse events. The biological activity observed in derivatives of Fallopia sachalinensis provides a scientific basis for the use of the plant in traditional medicines.

Pharmacokinetics of Valproic Acid in Guinea-pigs with Biliary Abnormality

To explore whether biliary cannulation, biliary obstruction or gall bladder obstruction could alter the disposition of valproic acid, guinea-pigs were subjected to common bile duct cannulation or ligation, gall bladder-neck ligation or sham surgery as a control. They were then given an intravenous (i.v.) dose of sodium valproate (50 mg kg-1) and the pharmacokinetics of valproic acid in each group were compared. In the cannulated group, significant decreases (P less than 0.05) in the area under the elimination curve (AUC), the volume of distribution at steady-state (Vdss) and the mean residence time (MRT) were observed. Significant increases (P less than 0.05) in the elimination rate constant (kz) and total clearance (CLtot) of valproic acid were noted. In the biliary obstructed guinea-pigs, the Vdss was significantly decreased (P less than 0.05). In the gall bladder obstructed guinea-pigs, there was a secondary peak of valproate plasma concentration, and the kz was significantly decreased. The biliary excretion of unchanged and conjugated valproic acid was 2.0 +/- 0.7 (s.e.m.) and 19.7 +/- 3.6 (s.e.m.)% of dose, respectively, and was almost completely reabsorbed in the enterohepatic recycling. Urinary excretion of unchanged and conjugated valproic acid, as well as non-conjugate metabolic clearance of valproic acid, were not significantly different among the four groups. The results suggest that the pharmacokinetics of valproic acid in guinea-pigs are particularly sensitive to interruption of the enterohepatic cycle. Biliary obstruction may elevate plasma concentrations owing to the decreased Vdss of valproic acid. Gall bladder obstruction may cause fluctuation of valproate plasma concentrations. The data indicate that the apparent total clearance of valproic acid is significantly less than the intrinsic clearance owing to enterohepatic recycling.

Studies on the magnitude and the mechanism of cough potentiation by angiotensin-converting enzyme inhibitors in guinea-pigs: involvement of bradykinin in the potentiation.

One adverse effect of the angiotensin-converting enzyme (ACE) inhibitors used for treatment of hypertension and congestive heart failure is the production of dry coughs. Imidapril is a new type of ACE inhibitor with a very low incidence of coughs. The magnitude and the mechanism of cough potentiation of imidapril and other ACE inhibitors has been studied in guinea-pigs. In normal guinea-pigs single and repeated dosing of imidapril at 0.1 to 100 mg kg-1 had no effect on capasaicin- or citric acid-induced coughs. Single and repeated dosing of enalapril and captopril at 10 to 30 mg kg-1, respectively, significantly increased the number of capsaicin-induced coughs. Repeated dosing of 1 mg kg-1 enalapril also significantly augmented the capsaicin cough. In bronchitic guinea-pigs imidapril also had no effect on the coughs induced by the two stimulants. Enalapril and captopril significantly increased the number of coughs induced not only by capsaicin but also by citric acid. Lower doses of enalapril were enough to augment the capsaicin-induced coughs, whereas medium to large doses failed to augment the cough irrespective of the protocol of administration. Bradykinin-induced discharges of the vegal afferents from the lower airway were significantly increased by enalaprilat but not by imidaprilat. Capsaicin-induced discharges of the afferents were, on the other hand, significantly depressed by enalaprilat, but not by imidaprilat. Interestingly, enalaprilat depression of the discharges was significantly reversed by Hoe-140, a bradykinin B2 receptor blocker. In guinea-pigs pretreated with a low dose of enalapril, arterial infusion of bradykinin significantly potentiated the coughs induced by capsaicin. The results indicated that imidapril was less potent than enalapril and captopril in potentiating cough responses induced by capsaicin and citric acid in guinea-pigs, and further suggest that bradykinin might be a key substance in the mechanism of the potentiation of coughs associated with ACE inhibitors.

In vivo anti-tussive activity and structural features of a polysaccharide fraction from water extracted Withania somnifera

Anti-tussive drugs are amongst the most widely used medications worldwide; however no new class of drugs has been introduced into the market for many years. The present study aims at evaluating the structural features and in vivo anti-tussive activity of a polysaccharide fraction from water extracted Withania somnifera. Herein, we have analyzed water extracted material of Withania somnifera using chemical, chromatographic, spectroscopic and biological methods. A polysaccharide fraction (F3) containing arabinosyl, galactosyl and galacturonosyl residues were obtained by anion exchange chromatography of the water extracted material. This polymer is branched and contained (1,5)-/(1,3,5)-linked arabinofuranosyl, (1,3)-/(1,6)-/(1,3,6)-linked galactopyranosyl residues together with small amount of terminal rhamnopyranosyl and terminal arabinofuranosyl residues. Peroral administration of this pectic arabinogalactan in a dose of 50 mg kg(-1) body weight (b.w.) decreased the number of cough efforts induced by citric acid in guinea pigs like that of codeine. This study provides a scientific basis for the past and present ethnomedical uses of this plant.

Structural features and antitussive activity of water extracted polysaccharide from Adhatoda vasica

Antitussive drugs are amongst the most widely used medications worldwide; however no new class of drugs has been introduced into the market for many years. Natural compounds offer interesting pharmacological perspectives for antitussive drug development. In this study, we have analyzed a pectic arabinogalactan isolated from Adhatoda vasica by aqueous extraction and precipitation with ethanol. This polysaccharide is branched and consisted mainly of 1,3-/1,3,6-linked galactopyranosyl and 1,5-/1,3,5-linked arabinofuranosyl residues. Peroral administration of this arabinogalactan (50 mg kg −1 body weight) inhibited the number of coughs induced by citric acid in guinea pigs and slightly decreased the values of specific airway resistance.

Suppressive effect of pectic polysaccharides from Cucurbita pepo L. var. Styriaca on citric acid-induced cough reflex in guinea pigs

Several water-soluble pectic polysaccharides were isolated from the pumpkin fruit biomass and characterized by composition, structural features and molecular properties. The pectic polysaccharides were tested for antitussive activity by studying the effects of citric acid-induced cough reflex in guinea pigs and reactivity of the airway smooth muscle in vivo conditions in comparison to the narcotic drug codeine. Oral administration of all pectic polysaccharides from pumpkin inhibited the number of coughs induced by citric acid in guinea pigs, but to various extents. The results indicated that the antitussive activity of the pectic polysaccharides is affected by their molecular and structural properties, whereby a synergistic action between the polysaccharide and non-carbohydrate components on the biological response has been suggested as well. The cough depressive efficacy of most of the tested polysaccharides was comparable and even higher than that of codeine. Moreover, the application of these polysaccharides provoked any side effects what is their advantage towards the conventional opioid-derived antitussive agents.

Differences between zofenopril and ramipril, two ACE inhibitors, on cough induced by citric acid in guinea pigs: role of bradykinin and PGE2

Dry and persistent cough is one of the commonest side effects experienced by patients treated with angiotensin-converting enzyme (ACE) inhibitors for the therapy of hypertension and congestive heart failure. The present study investigated the effect of zofenopril and ramipril on cough induced by citric acid in guinea pig and the involvement of bradykinin (BK) and prostaglandin E2 (PGE2) in mediating the responses of these drugs. Zofenopril (10mg/kg) or ramipril (3-10mg/kg), which is threefold more potent than zofenopril, on a mg basis, in lowering blood pressure, was orally administered daily in drinking water for 2weeks. At the end of this period, aerosol of citric acid solution (0.1M) was performed and the number of cough counted for 10min. The role of the kinin B(2) receptor was also investigated. BK and PGE2 levels in the bronchoalveolar lavage (BAL) fluid were measured after repeated oral treatment with zofenopril or ramipril (10mg/kg). Ramipril (3-10mg/kg) increased citric acid-induced cough by 40% and 60%, respectively, as compared to the vehicle control group (15.0 ± 1.8), while zofenopril (10mg/kg) was without effect. The enhancement of citric acid-induced cough caused by ramipril (10mg/kg) was reduced by the kinin B(2) receptor antagonist MEN16132 (0.25mg/kg ip). BK and PGE2 levels in the BAL fluid were increased, in comparison to the control group, after ramipril treatment, while they were unchanged after zofenopril administration. Zofenopril, contrary to ramipril, did not affect either citric acid-induced cough in the guinea pigs or BK and PGE2 production in the airways.

In vivo evaluation of the anti-asthmatic, antitussive and expectorant activities of extract and fractions from Elaeagnus pungens leaf

The leaf of Elaeagnus pungens thunb. (Family Elaeagnaceae) has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional Chinese medicine. This study was aimed at evaluating the anti-asthmatic, antitussive and expectorant activities in vivo of the ethanolic extract and fractions from the leaf of Elaeagnus pungens. The results showed that the 70% ethanolic extract increased the preconvulsive time of asthma induced by the combination of histamine and acetylcholine chloride in guinea pigs at the medium dose of 1.379 g/kg. The water fraction significantly prolonged the preconvulsive time ( P < 0.05) in guinea pigs, lengthened the latent period of cough ( P < 0.05) as well as decreased the cough frequency caused by citric acid in guinea pigs ( P < 0.01) and enhanced tracheal phenol red output in mice ( P < 0.01). Moreover, the petroleum ether fraction significantly reduced the cough frequency induced in guinea pigs ( P < 0.01) and improved tracheal phenol red output in mice ( P < 0.01). It indicated that the petroleum ether fraction and the water fraction of Elaeagnus pungens leaf were effective on anti-asthmatic, antitussive and expectorant activities, which supplied for further research on chemical constituents and pharmacological mechanisms.