Acid In Biological Material Research Articles

THE ESTIMATION OF NUCLEIC ACIDS IN SOME ALGAE AND HIGHER PLANTS

Several current methods for the extraction and estimation of nucleic acids in biological materials were applied to Euglena and other plants. The efficiency of both the preliminary extractions for acid-soluble-P and lipid-P and the subsequent extraction of the nucleic acids was studied. A relatively high acid concentration (15% TCA) was required to directly extract all the acid-soluble phosphates. These conditions appeared to remove a small amount of the RNA. Lower acid concentrations as used in the Ogur–Rosen method (2% PCA) failed to extract all the acid-soluble phosphates. By using a modification of the Ogur–Rosen initial extraction method, the acid-soluble phosphates were quantitatively extracted without loss of RNA. After removal of the acid-soluble phosphates and lipid phosphates, the plant nucleic acids were quantitatively extracted by either the Schmidt–Thannhauser or Schneider methods. In many of the plants tested, the presence of pentose-containing polysaccharides, protein degradation products, or polyphosphate (algae only) interfered in estimations based on either the Schneider or Schmidt–Thannhauser procedures. Such interfering substances in the Schmidt–Thannhauser method were eliminated by the use of an anion exchange resin. Details are given of a modified Schmidt–Thannhauser procedure which should be suitable for a wide range of plants. The modified procedure may be simplified for Euglena and some higher plant tissues depending on the nature and quantities of interfering substances present. Methods are also given for the quantitative separation of plant RNA nucleotides by paper chromatography and by ion exchange paper chromatography.

The determination of bile acids in bile and duodenal contents by quantitative paper chromatography bile acids and steroids 71

A method is described for the determination of bile acids in biological material by quantitative paper chromatography. It has been applied to the analysis of rat, rabbit and human bile and of human duodenal contents.

Effects of a new coronary vasodilator on the general and coronary hemodynamics and myocardial metabolism of man.

Article1 July 1959EFFECTS OF A NEW "CORONARY VASODILATOR" ON THE GENERAL AND CORONARY HEMODYNAMICS AND MYOCARDIAL METABOLISM OF MANELMERICE TRAKS, M.D., DONALD B. HACKEL, M.D., SALVATORE M. SANCETTA, M.D., F.A.C.P.ELMERICE TRAKS, M.D.Search for more papers by this author, DONALD B. HACKEL, M.D.Search for more papers by this author, SALVATORE M. SANCETTA, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-51-1-31 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe continued pharmacologic search for drugs capable of producing a positive increase in the coronary blood of man is an important phase in the management of patients with coronary artery disease. It is a distressing fact that many substances which pharmacologically appear to be promising later fail to fulfill this promise in the course of direct clinical trial. The clinical use of such "vasodilator" substances is often predicated upon the physiologic observation that they produce an increased coronary blood flow in animal preparations, without considering the possibility that the increase in flow may represent solely a heightened myocardial demand induced...Bibliography1. AhlquistHobentachWoodbury RPBRA: A pharmacologic study of N-methyl-N-cinnamyl-2-phenyl-propylamine, J. Am. Pharm. A. (Scient. Ed.) 38: 425, 1949. CrossrefGoogle Scholar2. BarkerSummerson SBWH: The colorimetric determination of lactic acid in biological material, J. Biol. Chem. 138: 535, 1948. CrossrefGoogle Scholar3. DaviesBrink PWF: Microelectrodes for measuring oxygen tension in animal tissues, Rev. Scient. Instruments 13: 524, 1942. CrossrefGoogle Scholar4. EckenhoffHafkenschiel JEJH: The effect of nikethamide on coronary blood flow and cardiac oxygen metabolism, J. Pharmacol. and Exper. Therap. 91: 362, 1947. MedlineGoogle Scholar5. EckenhoffHafkenschielLandmesser JEJHCM: The coronary circulation in the dog, Am. J. Physiol. 148: 582, 1947. CrossrefMedlineGoogle Scholar6. EckenhoffHafkenschielLandmesserHarmel JEJHCMM: Cardiac oxygen metabolism and control of the coronary circulation, Am. J. Physiol. 149: 634, 1947. CrossrefMedlineGoogle Scholar7. FoltzRubinSteigerGazes ELAWADC: The effects of intravenous aminophylline upon the coronary blood-oxygen exchange, Circulation 2: 215, 1950. CrossrefMedlineGoogle Scholar8. FriedemannHaugen TEGE: Pyruvic acid determination of keto acids in blood and urine, J. Biol. Chem. 147: 415, 1943. CrossrefGoogle Scholar9. GoodaleHackel WTDB: Measurement of coronary blood flow in dogs and man from rate of myocardial nitrous oxide desaturation, Circul. Res. 1: 502, 1953. CrossrefMedlineGoogle Scholar10. GoodaleLubinEckenhoffHafkenschielBanfield WTHJEJHWG: Coronary sinus catheterization for studying coronary blood flow and myocardial metabolism, Am. J. Physiol. 152: 340, 1948. CrossrefMedlineGoogle Scholar11. KienGomollSherrod GAAWTR: Coronary vasodilator action of cinnamyl vonedrine, Federation Proc. 15: 446, 1956. Google Scholar12. SilberKatz ENLN: Coronary dilators and angina. Reappraisal, J. A. M. A. 153: 1075, 1953. CrossrefMedlineGoogle Scholar13. Somogyi M: Determination of blood sugar, J. Biol. Chem. 160: 69, 1945. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Cleveland, Ohio*Received for publication September 15, 1958.From the Departments of Medicine and Pathology, Cleveland Metropolitan General Hospital and Western Reserve University, Cleveland, Ohio.†With the technical assistance of GLADYS HECKMAN, R. N., HANNA JANOUSKOVEC, R. N., and EDNA STONE, A. B., Cleveland, Ohio.Supported by grants from the U. S. Public Health Service (H-863) (Dr. Hackel), The William S. Merrell Co., and the Cleveland Area Heart Society (Dr. Sancetta), and performed in part during tenure of a Research Fellowship from the American Heart Association (Dr. Traks).‡Vasoflex is the trademark of the Wm. S. Merrell Co., Cincinnati, Ohio. It was supplied as a parenteral solution containing 5 mg./ml.Requests for reprints should be addressed to Elmerice Traks, M.D., Cleveland Metropolitan General Hospital, 3395 Scranton Road, Cleveland 9, Ohio. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited bySYMPATHOMIMETIC DRUGSIsoproterenol and cardiovascular performancePROBLEMS IN THE TREATMENT OF DECOMPRESSION SICKNESS (AND TRAUMATIC AIR EMBOLISM)Bibliography 1 July 1959Volume 51, Issue 1Page: 31-38KeywordsClinical trialsCoronary arteriesDrugsFellowshipsHeartHemodynamicsHospital medicineMedical servicesStable coronary artery diseaseVasodilators ePublished: 1 December 2008 Issue Published: 1 July 1959 PDF downloadLoading ...

The determination of aminophenols and aminobenzoic acids: A study of extraction procedures applicable to biological materials

Extraction procedures (solvent and ion-exchange resin) have been studied with regard to their potential use in the determination of aminophenols, aminobenzoic acids and 3-hydroxyanthranilic acid in biological materials.

The determination of uric acid in biological materials.

Research Article| February 01 1958 The determination of uric acid in biological materials A. E. Steel A. E. Steel 1The Royal Bath Hospital, Harrogate, Yorkshire Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1958) 68 (2): 306–309. https://doi.org/10.1042/bj0680306 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation A. E. Steel; The determination of uric acid in biological materials. Biochem J 1 February 1958; 68 (2): 306–309. doi: https://doi.org/10.1042/bj0680306 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1958 CAMBRIDGE UNIVERSITY PRESS1958 Article PDF first page preview Close Modal You do not currently have access to this content.

Determination of Pipecolic Acid in Biological Materials

Determination of Pipecolic Acid in Biological Materials

Studies on micro-colorimetric determination of phenylacetic acid in biological materials II

Studies on micro-colorimetric determination of phenylacetic acid in biological materials II

Colorimetric Determination of Trace Quantities of Boric Acid in Biological Materials

Colorimetric Determination of Trace Quantities of Boric Acid in Biological Materials

A RAPID, SIMPLE METHOD FOR THE DETERMINATION OF REDUCED, DEHYDRO-, AND TOTAL ASCORBIC ACID IN BIOLOGICAL MATERIAL

A RAPID, SIMPLE METHOD FOR THE DETERMINATION OF REDUCED, DEHYDRO-, AND TOTAL ASCORBIC ACID IN BIOLOGICAL MATERIAL

Studies on microcolorimetric determination of phenylacetic acid in biological materials. I

Studies on microcolorimetric determination of phenylacetic acid in biological materials. I

A criticism of the indiscriminate use of the Schmidt-Thannhauser method for the fractionation of nucleic acids in biological material.

A criticism of the indiscriminate use of the Schmidt-Thannhauser method for the fractionation of nucleic acids in biological material.

α-AMINOADIPIC ACID AS A CONSTITUENT OF A CORN PROTEIN

In a study of transaminase systems Braunstein (1) learned that α-aminoadipic acid takes part in transamination. Both pigeon breast muscle mince and a purified glutamic transaminase extracted from it were active in transferring an amino group from alanine to α-ketoadipic acid. A plant aspartic transaminase was inactive with the same substrate. Experiments by Borsook and Dubnoff (2) on the synthesis of arginine by rat kidney slices showed that, in addition to the activity of glutamic and aspartic acids in transferring amino groups to citrulline, lysine was active. They suggested at that time that lysine was converted to glutamic acid. Since aminoadipic acid was active in transamination, Braunstein suggested (3) that the lysine activity in arginine synthesis was due to its conversion into aminoadipic acid. Dubnoff and Borsook (4) repeated their previous experiments using aminoadipic acid as a substrate and found that it could aminate citrulline sufficiently well to explain the activity of lysine. That one path of lysine degradation in guinea pig liver homogenate was through α-aminoadipic acid was proved by Borsook et al. (5) using radioactive L-lysine as a substrate and isolating radioactive α-aminoadipic acid from the reaction mixture. The aminoadipic acid was further degraded to α-ketoadipic acid and glutaric acid (6). Geiger and Dunn (7) and Stevens and Ellman (8) independently found that aminoadipic acid was unable to replace lysine as an essential amino acid in rats and in two bacteria used in bioassays for lysine, Leuconostoc mesenteroides and Streptococcus faecalis. The occurrence of free aminoadipic acid in biological material raises the possibility that this amino acid may be a protein constituent. In the research described herein evidence is presented that a-aminoadipic acid is part of a natural protein.

The micro-estimation of benzoic and hippuric acids in biological material.

Research Article| February 01 1951 The micro-estimation of benzoic and hippuric acids in biological material F. Dickens; F. Dickens 1Courtauld Institute of Biochemistry, Middlesex Hospital Medical School, London, W. 1 Search for other works by this author on: This Site PubMed Google Scholar Joan Pearson Joan Pearson 1Courtauld Institute of Biochemistry, Middlesex Hospital Medical School, London, W. 1 Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1951) 48 (2): 216–221. https://doi.org/10.1042/bj0480216 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation F. Dickens, Joan Pearson; The micro-estimation of benzoic and hippuric acids in biological material. Biochem J 1 February 1951; 48 (2): 216–221. doi: https://doi.org/10.1042/bj0480216 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1951 CAMBRIDGE UNIVERSITY PRESS1951 Article PDF first page preview Close Modal You do not currently have access to this content.

SUBCLINICAL VITAMIN DEFICIENCY V. THE ASSAY OF SUBCLINICAL THIAMIN DEFICIENCY

Article1 September 1944SUBCLINICAL VITAMIN DEFICIENCY V. THE ASSAY OF SUBCLINICAL THIAMIN DEFICIENCYMILDRED CARLEEN HULSE, M.A., NORMAN WEISSMAN, PH.D., ELMER STOTZ, PH.D., MARSHALL CLINTON, M.D., JOSEPH W. FERREBEE, M.D.MILDRED CARLEEN HULSE, M.A.Search for more papers by this author, NORMAN WEISSMAN, PH.D.Search for more papers by this author, ELMER STOTZ, PH.D.Search for more papers by this author, MARSHALL CLINTON, M.D.Search for more papers by this author, JOSEPH W. FERREBEE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-21-3-440 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptINTRODUCTIONThe assay of subclinical thiamin deficiency is primarily a problem in defining changes in thiamin nutrition before signs or symptoms of deficiency make their appearance. Accomplishment of this end presupposes a knowledge of normal thiamin nutrition; that is, normal extracellular and intracellular thiamin concentrations, and the size and sequence of variation in these values which may precede the occurrence of clinical deficiency.1The purpose of the present study is to determine whether measurable changes in either extracellular or intracellular thiamin concentrations occur in subclinical thiamin deficiency. For comparison, observations are also reported on thiamin excretion and on pyruvate metabolism....Bibliography1. HULSEWEISSMANOWENFERREBEE MCNPSJW: Subclinical vitamin deficiency, Science, 1943, xcvii, 47. Google Scholar2. ATKINSCHULTZFREY LASCN: Ultramicrodetermination of thiamin by the fermentation method, Jr. Biol. Chem., 1939, cxxix, 471. CrossrefGoogle Scholar3. SCHULTZATKINFREYWILLIAMS ASLCNRR: Application of the sulfite cleavage of thiamin to the yeast fermentation method, Jr. Am. Chem. Soc., 1941, lxiii, 632. CrossrefGoogle Scholar4. HULSEWEISSMANROWLANDGROSSFERREBEE MCNVRJW: Subclinical vitamin deficiency. VI. Thiamine in skeletal muscle of infants and children, Am. Jr. Dis. Child., 1944, lxvii, 30. CrossrefGoogle Scholar5. HULSEWEISSMANFERREBEE MCNJW: Subclinical vitamin deficiency. IV. Plasma thiamin, Jr. Clin. Invest., 1944, xxiii, 297. Google Scholar6. KUTTNERLICHTENSTEIN TL: Micro colorimetric studies, II, Jr. Biol. Chem., 1930, lxxxvi, 671. CrossrefGoogle Scholar7. FERREBEECARDEN JWGA: A procedure for the routine determination of vitamin B1 in urine, Jr. Lab. and Clin. Med., 1940, xxv, 1320. Google Scholar8. ROBINSONMELNICKFIELD WDDH: Urinary excretion of thiamin in clinical cases and the value of such analyses in the diagnosis of thiamin deficiency, Jr. Clin. Invest., 1940, xix, 399. CrossrefGoogle Scholar9. BUEDINGWORTIS EH: The stabilization and determination of pyruvic acid in the blood, Jr. Biol. Chem., 1940, cxxxiii, 585. CrossrefGoogle Scholar10. LUPLATT GDBS: The metabolism of pyruvic acid in normal and vitamin B1-deficient states. V. The effect of exercise on blood pyruvate in vitamin B1 deficiency in man, Biochem. Jr., 1939, xxxiii, 1538. CrossrefGoogle Scholar11. BUEDINGSTEINWORTIS EMHH: Blood pyruvate curves following glucose ingestion in normal and thiamin-deficient subjects, Jr. Biol. Chem., 1941, cxl, 697. CrossrefGoogle Scholar12. STOTZBESSEY EOA: The blood lactate-pyruvate relation and its use in experimental thiamin deficiency in pigeons, Jr. Biol. Chem., 1942, cxliii, 625. CrossrefGoogle Scholar13. WILLIAMSMASONWILDERSMITH RDHLRMBF: Observations on induced thiamine (vitamin B1) deficiency in man, Arch. Int. Med., 1940, lxvi, 785. CrossrefGoogle Scholar14. FERREBEEWEISSMANPARKEROWEN JWNDPS: Tissue thiamin concentrations and urinary thiamin excretion, Jr. Clin. Invest., 1942, xxi, 401. CrossrefGoogle Scholar15. WILLIAMSMASONWILDER RDHLRM: The minimum daily requirements of thiamine of man, Jr. Nutr., 1943, xxv, 71. CrossrefGoogle Scholar16. JOHNSONDARLINGFORBESBROUHAEGAÑAGRAYBIEL RERCWHLEA: The effects of a diet deficient in part of the vitamin B complex upon men doing manual labor, Jr. Nutr., 1942, xxiv, 585. CrossrefGoogle Scholar17. FERREBEEWEISSMANPARKEROWEN JWNDPS: The thiamin content of human tissue, Res. Pub. Assoc. Nerv. and Ment. Dis., 1943, xxii, 42. Google Scholar18. PUCHERSHERMANVICKERY GWCCHB: A method to determine small amounts of citric acid in biological material, Jr. Biol. Chem., 1936, cxiii, 235. CrossrefGoogle Scholar19. SOBERLIPTONELVEHJEM HAMACA: The relation of thiamine to citric acid metabolism, Jr. Biol. Chem., 1940, cxxxiv, 605. CrossrefGoogle Scholar20. KREBS HA: The biochemical lesion in vitamin-B1 deficiency, Chem. and Ind., 1938, lvii, 213. Google Scholar21. EVANS EA: The biochemical action of the vitamins, 1942, The University of Chicago Press, Chicago. Google Scholar22. NAJJARHOLT VALE: Studies in thiamin excretion, Bull. Johns Hopkins Hosp., 1940, lxvii, 107. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Boston, Massachusetts*Received for publication September 20, 1943.From the Laboratory of Dental Medicine, Harvard School of Dental Medicine; the Medical Clinic, Peter Bent Brigham Hospital, and the Biochemical Laboratory of the McLean Hospital, Boston, Massachusetts.Aided by grants from the Williams and Waterman Fund, Research Corporation, New York City, and the Nutrition Foundation, New York City.This report is a preliminary study whose completion has been delayed by other work related to the war effort. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 September 1944Volume 21, Issue 3Page: 440-446KeywordsAttentionExcretionHospital medicineNutritionPyruvateResearch fundingResearch laboratoriesSigns and symptomsThiamine Issue Published: 1 September 1944 PDF downloadLoading ...

Micro-determination of isocitric and cis-aconitic acids in biological material

Research Article| January 01 1944 Micro-determination of isocitric and cis-aconitic acids in biological material H. A. Krebs; H. A. Krebs 1Department of Biochemistry, University of Sheffield Search for other works by this author on: This Site PubMed Google Scholar L. V. Eggleston L. V. Eggleston 1Department of Biochemistry, University of Sheffield Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1944) 38 (5): 426–437. https://doi.org/10.1042/bj0380426 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation H. A. Krebs, L. V. Eggleston; Micro-determination of isocitric and cis-aconitic acids in biological material. Biochem J 1 January 1944; 38 (5): 426–437. doi: https://doi.org/10.1042/bj0380426 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1944 CAMBRIDGE UNIVERSITY PRESS1944 Article PDF first page preview Close Modal You do not currently have access to this content.

Additions and Corrections - Existence of an Alkali-Stable Derivative of Pantothenic Acid in Biological Materials.

Additions and Corrections - Existence of an Alkali-Stable Derivative of Pantothenic Acid in Biological Materials.

THE COLORIMETRIC DETERMINATION OF LACTIC ACID IN BIOLOGICAL MATERIAL

THE COLORIMETRIC DETERMINATION OF LACTIC ACID IN BIOLOGICAL MATERIAL

Estimation of lactic acid in biological material by oxidation with ceric sulphate

Estimation of lactic acid in biological material by oxidation with ceric sulphate

Quantitative estimation of nicotinic acid in biological material

Quantitative estimation of nicotinic acid in biological material

A METHOD TO DETERMINE SMALL AMOUNTS OF CITRIC ACID IN BIOLOGICAL MATERIAL

A METHOD TO DETERMINE SMALL AMOUNTS OF CITRIC ACID IN BIOLOGICAL MATERIAL