Density, ρ, refractive index, nD, speed of sound, u, and viscosity, η, of the betaine + dl-lactic acid (1:2), betaine + dl-lactic acid (1:5), betaine + dl-lactic acid-water (1:1:1), betaine-levulinic acid (1:2), betaine-citric acid-water (2:1:6), l-proline + levulinic acid (1:2) and l-proline + dl-lactic acid (1:1) deep eutectic solvents were measured at several temperatures from 293.15 K to 343.15 K and at atmospheric pressure. From the experimental data, coefficients of thermal expansion, α, isentropic compressibility, ks, and molar refraction, Rm, were calculated. Moreover the thermal behavior of these DESs, from glass transition to degradation temperature, was studied by differential scanning calorimetry and thermogravimetric analysis.