Among many aspects of plant defence responses to pathogenic infection are changes in the composition of the exocellular matrix. To study potential defences in white lupin (Lupinus albus L.), suspension-cultured cells were treated for 24 h with one of three different elicitors : CuCl 2 , and two fungal elicitor preparations from purified cell walls of yeast and Colletotrichum lindemuthianum. Two subsets of exocellular proteins: ionicallybound wall proteins and proteins secreted into culture medium, were isolated, and their patterns compared following electrophoretic separation. Only a few proteins were observed in culture filtrates with dominating bands at 27, 33, and 42 kDa. About 30 proteins were observed in cell wall extracts. Changes in protein intensities evoked by elicitor treatments depended on the type of elicitor used, the age and composition of lupin cell culture, and concentration of applied fungal elicitor. Based on these observations, ten proteins were chosen for N-terminal sequencing, and sequences 5-30 amino acids long for nine proteins were obtained. Three of the major proteins sequenced were identified as acidic exocellular chitinase, polygalacturonase-inhibiting protein, and germin/oxalate oxidase.