In this article we present, for the first time, the results of 20 years field trials of triploid white poplar (Populus alba L.) and grey poplar (Populus x canescens Sm.) clones propagated by in vitro culture in different edaphic conditions (6 clones in total). The soils of the test plots differ in fertility, chemical and physical-chemical characteristics, which had a significant impact on the plants’ capacity for survival, health and growth. Low fertile, dry, acidic sandy loam and sandy soils are the least suitable for cultivating of in vitro micropropagated clones. In this case we had sandy loam soils on glaciofluvial monomineral quartz sands. The response to adverse growing conditions largely depended on the genotypic characteristics of the plants. The grey poplar clone named Hopersky 1 cultivar has shown the highest ecological plasticity as it has broad genetic basis (it is a hybridogenic species and allotriploid).