Acid Phosphatase Acp Research Articles

Effects of melatonin and metformin in preventing lysosome-induced autophagy and oxidative stress in rat models of carcinogenesis and the impact of high-fat diet

Imbalanced glucose tolerance and insulin resistance remain important as high cancer risk factors. Metformin administration to diabetic patients may be associated with a reduced risk of malignancy. The combined effects of the hormone melatonin and metformin in oncology practice have shown positive results. The relevance of our study is to find out the role of specific biomarkers of lysosome destruction and oxidative stress data in carcinogenesis models. The present study was designed to investigate the comparative synergic effect of peroral antidiabetic metformin (MF) and pineal hormone melatonin (MEL) administered alone and in combination in two different rat’s models of mammary tumour proliferation in vivo (N-methyl-N-nitrosourea, NMU or 7,12-dimethylbenz[a]anthracene, DMBA). We have studied the processes of lysosomal destruction (alanyl aminopeptidase AAP, leucyl aminopeptidase LAP, acid phosphatase AcP, β-N-acetylglucosaminidase NAG, β-galactosidase β-GD and β-glucuronidase β-GR) caused by evaluated oxidative stress in three types of tissues (liver, heart, and spleen) in female Sprague–Dawley rats fed a high-fat diet (10% of total fat: 2.5% from lard and 7.5% from palm olein). Our results revealed an increase in the activity of the studied lysosomal enzymes and their expression in a tissue-specific manner depending on the type of chemical agent (NMU or DMBA). MANOVA tests in our study confirmed the influence of the three main factors, type of tissue, chemical impact, and chemopreventive agents, and the combinations of these factors on the lysosomal activity induced during the process of cancerogenesis. The development and induction of the carcinogenesis process in the different rat models with the high-fat diet impact were also accompanied by initiation of free-radical oxidation processes, which we studied at the initial (estimated by the level of diene conjugates) and final (TBARS products) stages of this process. The combined effects of MEL and MF for the two models of carcinogenesis at high-fat diet impact for AAP, LAP, and AcP showed a significant synergistic effect when they impact together when compared with the effects of one substance alone (either MEL or MF) in the breast cancer model experiments. Synergistic effects of limiting destructive processes of lysosomal functioning β-GD enzyme activity we obtained in experiments with MEL and MF chemoprevention for both models of carcinogenesis for three tissues. The statistical SS test allowed us to draw the following conclusions on the role of each lysosomal parameter analyzed as an integral model: NAG > AcP > β-GD > β-GR > AAP > LAP.

Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme.

Compared with natural enzymes, nanozymes based on carbonaceous nanomaterials are advantages due to high stability, good biocompatibility, and the possibility of multifunctionalities through materials engineering at an atomic level. Herein, we present a sensing platform using a nitrogen-doped graphene quantum dot (NGQD) as a highly efficient fluorescent peroxidase mimic, which enables a colorimetric/fluorescent dual-modality platform for detection of hydrogen peroxide (H2O2) and biomolecules (ascorbic acid-AA, acid phosphatase-ACP) with high sensitivity. NGQD is synthesized using a simple hydrothermal process, which has advantages of high production yield and potential for large-scale preparation. NGQD with uniform size (3.0 ± 0.6 nm) and a single-layer graphene structure exhibits bright and stable fluorescence. N-doping and ultrasmall size endow NGQD with high peroxidase-mimicking activity with an obviously reduced Michaelis–Menten constant (K m) in comparison with natural horseradish peroxidase. Taking advantages of both high nanozyme activity and unique fluorescence property of NGQD, a colorimetric and fluorescent dual-modality platform capable of detecting H2O2 and biomolecules (AA, ACP) with high sensitivity is developed as the proof-of-concept demonstration. Furthermore, the mechanisms underlying the nanozyme activity and biosensing are investigated.

Effects of transportation stress on antioxidation, immunity capacity and hypoxia tolerance of rainbow trout (Oncorhynchus mykiss)

Transportation is common and necessary in aquaculture practice. A series of physiological changes in fish are caused by transportation stress. The present study investigated the effects of transportation of rainbow trout Onchorhynchus mykiss. Juvenile rainbow trout were placed in plastic bags, and sampled before transportation (P1), two hours after transportation (P2) and 48 h after transportation (P3). The water temperature, dissolved oxygen (DO), ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N) and nitrite nitrogen (NO2–-N) were analyzed. The relative expression of genes involved in stress and immunity (IL-1β, IL-8, TNF-α, LYZⅡ, HSP70 and HSP90), hypoxia tolerance (hif-1α, GLUT-1) and NF-kB pathway related (NF-kB, Ikb and Ikb2) were analyzed by RT-QPCR. In addition, oxidative damage, antioxidant and immune parameters (the activity of malondialdehyde MDA, superoxide dismutase SOD, catalase CAT and total antioxidant capacity T-AOC, acid phosphatase ACP, alkaline phosphatase AKP and total nitric oxide synthase NOS) were determined. The level of DO at P2 point was significantly decreased compared with P1 and P3 point. The NH4+-N concentration was significantly higher than that at P1 and P3. Further, there was a significant increase in HSP70 expression in gill and muscle during transportation. The expression of TNF-α, IL-1 β and IL-8 in gill and IL-1 β and IL-8 in muscle were significantly up regulated during transportation. The HSP70/90, TNF-α, IL-1 β and IL-8 genes can be used as bio-indicators to monitor stress. Moreover, ACP enzyme activity in liver and muscle, AKP in gill and MDA contents in gill and muscle were significantly higher at P2. The activity of NOS and T-AOC in gill was significantly higher at P3, and showed an increasing trend. In general, this study provides basial information of transportation, and will be helpful to choose more suitable conditions for successful transportation of juvenile rainbow trout.

Impact of Technogenic Saline Soils on Some Chemical Properties and on the Activity of Selected Enzymes

The study was based on saline soils with surface mineral layers impacted by the waste produced by the soda plant in Poland. The activity of selected enzymes (catalase CAT, alkaline AlP, and acid phosphatase AcP), pH in KCl, content of the clay, total organic carbon (TOC), total nitrogen (TN), total exchangeable bases (TEB), electrical conductivity (ECe), CaCO3, and concentration of available phosphorus AP were investigated in the soil next to the soda plant. Based on the enzyme activity, the following were calculated: enzymatic pH indicator AlP/AcP, the resistance index (RS), resilience index (RL), relative changes (RCh), and the time index (TI). The soil was sampled from the mineral horizon in spring and autumn from eight (S1–S8) soil sampling sites in the area of the soda plant and from the control point (C). Soil is characterized by alkaline reaction. Statistical analysis (ANOVA, η2 effect size) showed significant variation in parameters under the influence of different sites next to soda plant. The content of TOC ranged from 4.70 to 47.7 g kg−1, and TN from 19 to 4.36 g kg−1. ECe next to the soda plant ranged between 6.87 and 204 mS cm−1. The highest values were confirmed in the soil of S1 both in spring and autumn. Higher TEB values were noted in the soil in autumn. In the soil within the impact of the soda plant, the AP content decreased and in the soil from sites S1, S3, S6, and S8 (in spring), the lowest AP content was recorded at 1.20, 4.14, 5.98, and 0.99 mg kg−1, respectively. The highest activity of AlP in spring was noted at site S1 and in autumn at site S4. In the soil next to the soda plant, the activity of AcP decreased, as compared to the control which is seen from the negative values of the coefficient of relative changes (RCh). The analysis of RCh in the catalase activities showed that in the soil from sites S1, S2, S3, S4, and S5, the activity increased, as compared to the control. The lowest values of the resistance index (RS) for phosphatases were reported in the soil of S6. Research shows that the activity of enzymes and their indexes make it possible to conduct long-term monitoring and identify the processes in soil.

Genetic markers in primary open-angle glaucoma.

To investigate possible associations between genetic markers and Primary Open-Angle Glaucoma (POAG). A number of genetic markers were typed in 84 unrelated patients with POAG and compared with a random sample of healthy individuals. The markers were Transferrin, Group Specific Component, G1m (1), G1m (2) and G3m (5) Allotypes, Adenylate Kinase, Adenosin Deaminase, Glyoxalase I and Acid Phosphatase and PCR-based markers HLA-DQA1 and D1S80. No significant differences were found except the strong association between the group of POAG patients and Acid Phosphatase ACP*C allele (chi 2 = 32.86; p < 0.0001). Since Acid Phosphatase gene is localized to chromosome 2p23, this result could be a first comprehensive step in the localization of POAG genes.