Dye Acid Orange Research Articles

ZnCr-LDHs with dual adsorption and photocatalysis capability for the removal of acid orange 7 dye in aqueous solution

Abstract This study aims to explore the potential of layered double hydroxides (LDHs) materials for the treatment of contaminated waters by organic pollutants, using a combined dual process, namely adsorption-photocatalysis. A series of LDHs of ZnCr-X where (X = Cl, SO4 and CO3) were synthesized and characterized by several techniques such as DRX, FTIR, BET, TEM, and UV-Visible spectrophotometry. The pollutant acid orange 7 (AO7) is efficiently adsorbed on the surface of the three LDHs. The adsorption depends on the nature of the intercalated anion. The adsorption percentage of AO7 achieved for the intercalated anions SO 4 2 - , CO 3 2 - and Cl−, was 25, 32 and 49.5% respectively. We find that ZnCr–SO4 LHD is the most effective photocatalyst for AO7 dye decomposition in the UV and visible range and its photocatalytic activity is preserved during three cycles of photocatalytic tests. Such remarkable properties allow considering ZnCrSO4-LDH as a promising photocatalyst with high activity, long-term durability and excellent applicability.

Experimental investigation on dye wastewater treatment with reverse electrodialysis reactor powered by salinity gradient energy

A novel advanced oxidation process technology for organic wastewater treatment was proposed in this paper. The working principle is that a low-grade heat (LGH) is first converted into a salinity gradient energy (SGE), and then the SGE is converted into the degradation energy of organic pollutions by a reverse electrodialysis (RED) reactor. To verify the feasibility of converting a SGE into a degradation energy by a RED reactor, an experimental investigation of dye wastewater treatment with a RED reactor powered by SGE had been done. The results showed that the output current of RED reactor, operating parameters of electrode rinse solution (ERS) and degradation circulation modes have effects on the decolorization of acid orange 7 (AO7) dye wastewater. Under the present experimental conditions, after 20 min treatment, the decolorization efficiencies of AO7 dye wastewater for the independent degradation circulation mode reached 99.93% and 96.52% in the anodic and cathodic loops respectively, while that for the synergetic degradation circulation mode reached 82.85%.

Bio-based Substances From Compost as Reactant and Active Phase for Selective Capture of Cationic Pollutants From Waste Water.

Alumina porous monoliths were successfully fabricated using a simple and reproducible synthesis dispersing gamma alumina phase from commercial boehmite (GAB) in water containing water-soluble bio-based substances (BBSs) obtained from composted biowaste. The wet mixture obtained was shaped in form of small spheres and then dried and calcined at 500°C in order to burn the organic matter and obtain mesoporous monoliths. They were successively functionalized with BBSs in order to introduce BBS functional groups and obtain an efficient adsorbing system. Therefore, in this work, BBSs acted as template/binder for the production of monoliths and as functionalizing agent of the produced monoliths. The reference powders, deeply studied in a published article (Sadraei et al., 2019b), and the monoliths of GAB before and after functionalization were characterized by means of x-ray diffraction to evidence their crystal structure, Fourier transform infrared spectroscopy for evaluating the presence of BBSs on the supports, thermogravimetric analysis to measure the thermal stability of the materials and quantify the functionalizing BBS amount immobilized on the supports, nitrogen adsorption at 77 K for the investigation of the surface area and porosity of the systems, and zeta potential measurements to analyze the effect of BBS immobilization on the surface charge of the supports and to predict the type of interaction, which can be established with substrates. Finally, the systems were applied in removal of pollutants with different charge, polarity, and molecular structure, such as dyes (crystal violet and acid orange 7) and contaminants of emerging concern (carbamazepine and atenolol). Only the cationic dye CV is captured by the adsorbing material, and this allows envisaging a possible use of the functionalized monoliths for selective adsorption of cationic substrates.

Quaternary‐Ammonium‐Based Gels with Varied Alkyl Chains for the Efficient Removal of Toxic Acid Orange 7

AbstractWe report the design and synthesis of thiol‐norbornene based ingenious gels with pendent quaternary ammonium substituents on the norbornene backbone that are conducive to remove toxic anionic azo dye acid orange 7 (AO7) from wastewater. Three different alkyl side chains on the dangling quaternary ammonium moiety were chosen prudently and their performance towards removal of azo dye was scrutinised. The characterisation of all the precursor monomers and the ensuing gels were accomplished by 1H, 13C NMR and Mass spectrometry. The influence of extent of crosslinking on the solvent uptake ability was investigated and the swelling nature of the gels were correlated with the solubility parameter of different solvents. The surface morphology of the gels as visualised by FE‐SEM analysis, revealed macropores in the range 4–12 μm. The relationship between the composition of quaternary ammonium moiety on the gels and their viscoelastic properties was realised. The dye removal kinetics as observed from UV‐Vis spectra were best correlated with the pseudo‐second‐order model and the adsorption isotherm of dye removal process was best fitted with the Langmuir isotherm model with high correlation coefficient. The material was found to be fairly recyclable up to 10th cycles with loss of only 8% efficiency. To the best of our knowledge, this is the first ever report on the removal of anionic dye based on thiol‐norbornene photo crosslinked network. We believe that this design and composition‐property relationship will enthuse the researchers to develop efficient chemical adsorbents based on environmentally benign systems.

Adsorption of acid orange 7 using green synthesized CaO/CeO2 composite: An insight into kinetics, equilibrium, thermodynamics, mass transfer and statistical models

The present research reported the co-precipitation mediated synthesis of mixed metallic oxide composite, CaO/CeO2 using ultrasound-assisted extract of Eichhornia crassipes leaves. Fourier Transform Infrared Spectroscopy (FT-IR), Thermo-gravimetric/Differential Thermal analysis (TG/DTA), X-ray Diffraction (XRD), and Brunauer-Emmet-Tellet (BET) isotherms ensured the formation of the mixed oxide. Further, the CaO/CeO2 composite was tested in the anionic azo dye, Acid Orange 7 (AO7) removal process. The optimal removal of 92.68% was attained in the initial solution pH of 2.0 with the composite dosage of 0.1 g for 10 mg/L of AO7 concentration, operating at the temperature of 301 K. Response Surface Methodology (RSM) and Artificial Neural Network (ANN) techniques were employed to model the dye removal process. A second-order quadratic model from Box-Behnken Design (BBD) predicted and optimized the dye removal percentage with high degree of statistical accuracy (Fcal > Ftab at df=9; p < 0.0001). Likewise, a three-layered ANN model using the Levenberg–Marquardt backpropagation algorithm well predicted the dye adsorption process with the least root mean square error values (RMSE 0.3020). Further, the mean impact value (MIV) method identified pH0 as the most influential batch variable in the AO7 dye adsorption process.

Adsorption behavior and mechanism of acid orange 7 and methylene blue on self-assembled three-dimensional MgAl layered double hydroxide: Experimental and DFT investigation

The removal of toxic ionic dyes is an urgent issue in the water pollution control field. Herein, the self-assembled three-dimensional MgAl layered double hydroxide (3D-MA-LDH) have been synthesized via the soft-template synthesis employing sodium dodecyl sulfate (SDS) as soft template and can be successfully applied in the anionic azo dye Acid Orange 7 (AO7) and cationic heteroaromatic compound methylene blue (MB) removal. The maximum removal capacities were 485.6 and 58.3 mg·g−1 for AO7 and MB, respectively. The removal behavior for AO7 and MB fitted the Langmuir and Freundlich model, respectively, and revealed a diffusion process contained chemical adsorption. X-ray diffraction, Fourier transform spectroscopy, and X-ray photoelectron spectroscopy demonstrated that anion exchange dominated the AO7 removal, while MB was adsorbed via hydrophobic interaction between MB and DS. Density functional theory further confirmed the interaction, wherein the bonding distance was 1.622 Å between AO7 and 3D-MA-LDH’s layer. MB showed strong affinity toward DS with no dependence of bond connection to be stably anchored on the 3D-MA-LDH. In addition, the recyclability experiments exibited that 3D-MA-LDH could be stably reused for at least five times. This work provided a new approach for cleaning ionic dyes and broadened the application field of 3D- LDHs.

Degradation of AO7 with Magnetic Fe3O4-CuO Heterogeneous Catalyzed Sodium Percarbonate System

Magnetically recyclable Fe3O4-CuO was synthesized by a one-step hydrothermal method and characterized by scanning electron microscopy coupled with energy dispersive spectrometer (SEM-EDS) and X-ray diffraction (XRD). The degradation of azo dye acid orange 7 (AO7) by percarbonate (SPC) activated with Fe3O4-CuO was studied. The effects of Fe3O4-CuO catalyst loading, SPC concentration, pH value, and common chloride ions on AO7 degradation in the Fe3O4-CuO/SPC system were evaluated. The main reaction mechanism of AO7 degradation was analyzed. The results show that Fe3O4-CuO could effectively activate SPC to degrade AO7 and the reaction was accelerated with the increase of Fe3O4-CuO dosage. The increase of SPC dosage was favorable for the degradation of AO7, but excessive SPC dosage inhibited the degradation of AO7. Common ions (e.g., Cl-) in dye wastewater could promote the degradation of AO7, and the degradation rate increased with increasing concentration of Cl-. The reaction mainly occurred on the surface of the catalyst, and·OH was identified as the main active species for the degradation of AO7. The catalyst Fe3O4-CuO showed excellent stability owing to the high catalytic activity remaining after 4 cycles of repeated use. The Fe3O4-CuO/SPC system achieved a high mineralization rate in the process of decolorization of AO7.

Enhancing the acid orange dye degradation efficiency of Mg-based glassy alloys with introducing porous structure and zinc oxide

Abstract Mg-based alloys exhibit a good degradation performance in wastewater treatment containing dyes. The degradation performance of as-spun Mg crystalline ribbon, Mg78Zn22 (MZ) semi-amorphous ribbon and Mg73Zn22Ca5 (MZC) amorphous ribbon on acidic orange dye (AO II) solution is improved apparently by citric acid (CA) solution immersion. The degradation rate constant k of MZ ribbon after immersing for 1 h reaches 0.084 min−1, showing a much higher degradation efficiency than other immersed ribbons. Compared with the immersed Mg and MZC ribbons, the surface of immersed MZ ribbon has a developed loofah porous structure and an appropriate crystallization. Here, the crystallization induced porous structure is composed of Zn and ZnO, which can generate more nascent hydrogen [H] by transporting Mg atoms to surface and more O 2 • − radical by supplying photo-catalytic ZnO under visible light and then enhance the degradation of AO II dye. This work not only proposes a new method to improve the degradation efficiency of AO II dye by Mg-based alloys, but also provides a new insight into the microstructure and properties of Mg-based alloys.

Novel quantification of formation trend and reaction efficiency of hydroxyl radicals for investigating photocatalytic mechanism of Fe-doped TiO2 during UV and visible light-induced degradation of acid orange 7

A detailed mechanistic investigation of the hydroxyl radical (•OH) formation and organic pollutant degradation over transition metal-doped and undoped TiO2 photocatalysts was performed by the quantitative measurement of •OH and the identification of intermediate products under various experimental conditions. The Fe-doped TiO2 as a typical subject was prepared, characterized and used to degrade an azo dye Acid Orange 7 (AO7). It is indicated that the enhanced photocatalytic activity of Fe-doped TiO2 for AO7 degradation was attributed to the increase in surface area, the facilitated charge transfer via Fe-dopant, and a red shift of absorbable wavelength, maintaining a great formation of •OH under visible irradiation. The oxidation of H2O by holes was estimated as the major pathway of •OH formation rather than the reduction of dissolved O2 by electrons, and their formation trends reached to approximately 75% and 25%, respectively. Meanwhile the synergistic effect of Fe-dopant produced nearly 10% of extra •OH by visible light photoactivation. The intermediate products and pathways of AO7 degradation varied greatly with different photocatalysts and conditions of the process, involving several reaction mechanisms such as the azo bond cleaving, naphthalene oxidation, desulfonation, and hydroxylated products generation. Through the quantification of •OH-reacted efficiency we proposed, a stoichiometry of •OH affecting overall reaction mechanisms in the TiO2-assisted photodegradation of AO7 was further established. This study can provide new insights on how to better clarify the variation regularity of organic pollutant degradation from different treatments of the •OH-based advanced oxidation processes.

The efficient removal of the hazardous azo dye Acid Orange 7 from water using modified biochar from Pea-peels

The efficient removal of the hazardous azo dye Acid Orange 7 from water using modified biochar from Pea-peels

Lanthanum (III) incorporated chitosan-montmorillonite composite as flexible material for adsorptive removal of azo dyes from water

La (III) incorporated chitosan-montmorillonite (CS-La3+-MMT) composite was prepared and characterized using FTIR, XRD, TGA, EDX and SEM analysis. The surface area, pore volume and the pore size distribution of the CS-La3+-MMTwere calculated using the BET technique. The dye adsorption efficiency of the composite was evaluated using an aqueous solution of (50 mg/L) reactive red (RR) and acid orange (AO) dyes. The extent of adsorption in initial pH value of the solution, contact time, and initial concentration of dye were also investigated. The optimal conditions for removal of dyes were found to be: molar ratio of (CS-La3+-MMT), 1:1; pH: 4; temperature: 30 °C for 40 min in 50.00 mL of 50 mg L−1of dye solutions and 100 mg of adsorbent. The results indicated that the adsorption capacity of the CS-La3+-MMT composite was higher than the average values of those of CS, MMT, La3+ and CS/MMT. The isotherms of the adsorption process were also examined. The adsorption process were in good agreement with the Langmuir adsorption isotherm model fitting. The highest sorption efficiency of RR and AO dyes on the CS-La3+-MMT was attributed to the highly porous nature of the composite in addition to their ability to form hydrogen bond between free OH groups present in the composites with the dye molecules.

Optimization of Suitable Eco-Friendly Technology for Bioremediation of Textile Wastewaters

Water is one of the major products of nature used enormously by human beings and it is not unnatural that any growing community generates enormous waste water or sewage. As a clean environment is a prerequisite for a healthy living in any urban settlement, proper treatment and safe disposal of sewage call for prime attention. Untreated waste water can cause pollution of surface and ground waters. Many new developments in the field of sewage treatment are eventually taking place. These developments include improvements for more effective removal of pollutants and new treatment processes capable of removing pollutants not ordinarily removed by conventional methods. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa and Scenedesmus obliquus) biofuel production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Discharge of untreated domestic and industrial wastewater into aquatic bodies is posing a serious eutrophication threat, leading to a slow degradation of the water resources. A number of physical, chemical and biological methods have been developed for the treatment of wastewaters; among these, the use of microalgae is considered as a more eco-friendly and economical approaches.

Degradation of Azo Dye (Acid Orange 7) in a Microbial Fuel Cell: Comparison Between Anodic Microbial-Mediated Reduction and Cathodic Laccase-Mediated Oxidation

More than 80 per cent of wastewater from industries is discharged into receiving water bodies without any pollution control. Microbial fuel cells (MFCs) are a promising technology for the simultaneous treatment of wastewater and electricity production. With regard to azo-dye containing wastewater (e.g. from textile manufacturing), the dye may be fed via the anode chamber containing electrochemically active bacteria or via the cathode chamber containing laccase enzyme as catalyst for oxygen reduction. This study investigated which of the two approaches is the best with regard to rate of decolourization of the dye (Acid orange 7), COD reduction and electricity production. The power density was higher for the MFCDye cathode (50±4 mW m-2, COD reduction 80.4±1.2%) compared with 42.5±2.6 mW m-2 (COD reduction 69±2%) for MFCDye anode. The time required for decolourization was longer in the MFCDye anode (Shewanella oneidensis) where only 20% decolourization was obtained after 24 h compared to 80% for the MFCDye cathode. The anodic dye degradation products were unstable when exposed to air resulting in regaining of colour. In case of degradation by laccase in the cathode chamber, the decolourization products were stable and simpler in chemical structure as determined by GC-MS. This work suggests that feeding azo dyes in cathode chambers of MFCs containing laccase is a better way of treating the dyes compared to the commonly used approach of feeding the dye in the anode chamber provided enzyme activity can be sustained.

Adsorption mechanism of acid orange 7 on photocatalytic materials based on TiO2

ABSTRACTThe degradation of organic molecules in an aqueous medium using heterogeneous photocatalysis depends on the chemical composition and concentration of the organic compound, the crystalline and morphological nature of the photocatalyst, the pH of the dye dilution, and the reaction temperature. Since photocatalytic degradation is a process that occurs on the surface of the catalytic material, it is desirable to induce maximum adsorption of the organic compound. One strategy to achieve this is to functionalize the surface of the catalyst to retain the molecule of interest. In this work, we studied the interaction of acid orange 7 (AO7) with commercial TiO2-anatase powder catalyst, and with a catalyst synthesized in house using titanium tetrachloride and ethanolamine (TiO2-et). Our results indicate that there is no adsorption of the AO7 dye on the TiO2-et particles. The infrared spectrum of the TiO2-et particles is presented.

Photoelectrocatalytic activity of spray deposited Fe2O3/ZnO photoelectrode for degradation of salicylic acid and methyl orange dye under solar radiation

Fe2O3/ZnO thin films are synthesized onto glass and fluorine doped tin oxide (FTO) coated glass substrates by simple spray pyrolysis technique. The effect of ZnO layer on structural, optical, morphological and photocatalytic properties of films has been studied. All synthesized films are polycrystalline having rhombohedral and hexagonal crystal structures for Fe2O3 and ZnO phases respectively. The maximum photoelectrochemical performance [(Isc = 0.884 mA) and (Voc = 980 mV)] has been observed at 60 ml quantity of ZnO solution. Photocatalytic and photoelectrocatalytic degradation performances of large area (100 cm2) Fe2O3/ZnO photoelectrode employed for degradation of salicylic acid (SA) and methyl orange (MO) dye under solar light illumination has been studied. It is observed that 80 % of SA and 98 % of MO dye get degraded in 320 min and 80 min respectively. The mineralization of SA and MO dye are studied by measuring chemical oxygen demand (COD) values.

Hydrodynamics of up-flow hybrid anaerobic digestion reactors with built-in bioelectrochemical system

Understanding the electrode configuration is vital for the successful application of bioelectrochemical system (BES) in recalcitrant wastewater treatment. Especially in those traditional anaerobic processes that integrate with BES to construct effective hybrid bioreactors. Hybrid bioreactors employed granular graphite as electrode material achieved 86.62 ± 1.83% decolorization efficiency of azo dye acid orange 7 (AO7) at influent AO7 loading rate of 800 g/(m3∙d) and it was about 6% higher than that with carbon fiber brush electrodes. Such electrodes were positioned above the anaerobic sludge layer and higher efficiency (8%) than the reactors with electrodes placed beneath the sludge layer was observed. Tracer experiments and modeling of residence time distribution indicated that the fluid pattern in hybrid bioreactors was modified to plug flow pattern and had a better consummate mixing ability compared to the conventional anaerobic reactor. Simulation using computational fluid dynamics technique showcased favorable mass transfer near electrode modules. The hydrodynamics of simulation and experimental results were connected by simplifying electrode module as a porous media model. This study thus proved that hybrid bioreactors can effectively enhance wastewater treatment comprehensively through the analysis of decolorization performance and hydrodynamics.

Efficient azo dye wastewater treatment in a hybrid anaerobic reactor with a built-in integrated bioelectrochemical system and an aerobic biofilm reactor: Evaluation of the combined forms and reflux ratio

A combined process that consisted of a hybrid anaerobic reactor (HAR) with an integral bioelectrochemical system and aerobic biofilm reactor (ABFR) was established for simulated azo dye wastewater treatment (domestic wastewater containing dye acid orange 7). The split combination form that separated HAR and ABFR into two individual reactors recorded a decolorization efficiency of 81.23 ± 0.12%, which was about 8% higher than that HAR and ABFR were stacked together into a single up-flow reactor. Implementation of reflux improved the decolorization and chemical oxygen demand (COD) removal in both the processes. Decolorization efficiency achieved 97.52 ± 0.66% in split process at a reflux ratio of 1 and the COD was 89 ± 2 mg/L in the final effluent. Further increasing the reflux ratio to 3 did not have any significance in treatment performance of the reactors. This study comprehensively revealed the influence of combination forms and reflux ratio on the performance of combined process.

Removal of Acid Orange 7 dye from aqueous solutions using polyaniline-modified rice bran: isotherms, kinetics, and thermodynamics

Background: Today, due to increasing usage of dyes in various industrials and their destructive effects on health and environment, it is necessary to remove them from industrial wastes. Although there are few studies on the use of rice bran modified with polyaniline (RB/PANI) for removal of different dyes, but the effect of this adsorbent on the removal of Acid Orange 7 (AO7) dye has not been evaluated yet. Therefore, this study was conducted to investigate the removal of AO7 dye by RB/PANI as an adsorbent. Methods: The adsorbent characteristics were determined using scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy. Also, the adsorbent surface area was measured by Brunauer–Emmett–Teller (BET) technique. The method of one-factor-at-a-time was used to optimize various factors including pH, temperature, and adsorbent dosage. Results: The optimal values for the factors affecting AO7 dye removal were calculated. It was revealed that the maximum dye removal was obtained at pH = 3, temperature = 25˚C, dye concentration = 30 mg/L, adsorbent dosage = 30 mg/L, and contact time= 60 minutes. The maximum removal percentage for RB/PANI was 97.13%. It was also revealed that Langmuir isotherm is the best fitted isotherm model. Conclusion: According to the results, the polyaniline-modified rice bran could be used as an excellent adsorbent for the removal of AO7 from aqueous solutions. The maximum dye removal efficiency for AO7 was obtained at pH = 3. Also, it was revealed that AO7 dye removal follows the pseudo-secondorder kinetic model and it is a spontaneous process.

Immobilisation of TiO2-P25 on a glass fibre mat: Preparation, photocatalytic activity and stability

The aim of this research was to produce an efficient catalytic system using a commercial photocatalyst immobilised onto a readily available support. The priority was a high catalytic activity and stability for the catalytic system as well as the simplest possible methodology for its preparation. The photocatalytic activity was verified by monitoring the decolourization of an anionic dye (Acid Orange 7) as a model molecule and antibiotics (sulfa-drugs mixture) under UVa and sunlight irradiation in distilled water and in artificial wastewater. The catalytic system stability was evaluated under cyclic and continuous processes. The developed optimised catalyst system contained one layer of TiO2-P25 immobilised onto a commercial glass fibre mat. This system was very simple to produce, did not require calcination prior to use and possessed a high catalytic activity, good resistance to leaching and good stability. The photocatalytic activity of the immobilised catalyst can grow asymptotically during work-up. In the cyclic process, a high activity of the catalytic system was maintained during the 11-fold use. Moreover, there was no reduction in the activity in this process at a load of 12 m3 of the Acid Orange 7 solution per 1 m2 of mat of the immobilised catalyst. The photocatalytic activity of fibreglass mat coated with TiO2 was also confirmed during exposure to sunlight.

Spectroscopic and Biophysical Interaction Studies of Water-soluble Dye modified poly(o-phenylenediamine) for its Potential Application in BSA Detection and Bioimaging

Ultrasound-assisted synthesis of water soluble poly(o-phenylenediamine) (POPD) and its doping with Acid Orange (AO), Fluorescein (Fluo) and Rhodamine-6G (R6G) dyes was carried out with a view to enhance the photophysical properties of POPD. XPS studies confirmed that doping of POPD occured through hydrogen bonding between NH group of POPD and C=O/SO−, S=O groups of the dyes. The presence of strong hydrogen bonding was also confirmed via UV-vis studies by the addition of urea and sodium chloride to the dye modified POPD adducts. Molar extinction coefficient of these adducts was found to bear a close relationship with the molecular structure. Fluorescence life time, (τf,) was found to be lowest (1.8 ns) for AO-POPD and highest (3.2 ns) for Fluo-POPD. The structure of AO-POPD was more strained, while that of Fluo-POPD was least strained. Intrinsic fluorescence decay constant, (k0f) showed increasing values for POPD, AO-POPD, Fluo-POPD, R6G-POPD as 0.071, 0.072, 0.153, and 0.172 (108 s−1), which could be correlated to the increasing strain-free molecular structure of the adducts. Circular dichroism spectra (CD) of BSA in presence of POPD and R6G- POPD revealed that it partially broke its helical structure, while Fluo-POPD and AO-POPD showed enhancement in the helical content. The 3-D fluorescence studies confirmed enhancement in hydrophobicity of POPD and R6G- POPD and increase in hydrophylicity of AO-POP and Fluo-POPD in the microenvironment of tryptophan residue-213 of BSA. Fluo-POPD and R6G-POPD adducts were chosen to find out the lowest detection limit (LOD) of BSA by differential pulse voltammetry (DPV) which was found to be 1.35 nM, and 1.65 nM using Fluo-POPD and R6G -POPD respectively. The binding constant of BSA with Fluo-POPD- and R6G-POPD was obtained as 3.98 × 106 Lmol−1 and 5.27 × 102 Lmol−1. These polymers could therefore, be used for the detection of BSA. Live cell imaging revealed that POPD nanoparticles were bound to the outer membrane of E. coli, while R6G-POPD, showed penetration into the cytoplasm and excellent labeling of E. coli. This facile technique could be used to design tunable biomarkers by tailoring the conjugated polymer with a desired dye molecule.