After reviewing a number of researches that demonstrate the effectiveness of a number of compounds containing the acetylenic amine group as antimicrobials and antiproliferative activity, our goal is to prepare new acetylenic amine derivatives from the compound 2-(2-((2,6- dichlorophenyl)amino)phenyl)acetic acid using the Mannich reaction by the react salt of a carboxylic acid with propargyl bromide to produce the compound (prop-2-yn-1-yl 2-(2-((2,6- dichlorophenyl)amino)phenyl)acetate) (1), which was refluxed with a number of secondary amines and formaldehyde in presence of the copper chloride as a catalyst to obtain 7 compounds (4-(dialkylamino)but2-yn-1-yl 2-(2-((2,6-dichlorophenyl)amino)phenyl)acetate)(2-8). The physical and spectral properties of the prepared compounds were measured, and the bioassay conducted for some substances by investigating their antibiotic activity. The two compounds (1,2) were also selected to test their anticancer activity against one of its types, a breast cancer cell line (MCF-7), using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using multiple concentrations compared to the normal cell line (WRL-68). The previous results showed the effect of the two compounds on the cancer cell line more than its effect on the normal cell line, the results showed significant differences (p≤0.0001) by calculating the (IC50) when treating compounds (1,2) for MCF-7 cancer cells at (250,300) µg/ml and for normal cells at (983,1292) µg/ml respectively. It is believed that the reason for its effectiveness is because contains acetylenic amine in compound (1) in addition to morpholine ring in compound (2), which research has proven to be effective in multiple medicines. Some pharmacokinetic activities of the prepared compounds have been predicted to support the discovery of new drugs using the Swiss ADME website.