Acetylcholinesterase In Rats Research Articles

Mechanisms of recovery of brain acetylcholinesterase in rats during chronic intoxication by isoflurophate.

During chronic intoxication by diisopropyl fluorophosphate (Isoflurophate, DFP; s.c. treatment on alternate days - first dose of 1.1 mg/kg, subsequent doses of 0.7 mg/kg each until the 23rd day) a partial recovery of enzymatic activity was found at 24 h after each DFP administration. Relative to maximal AChE depression at 90 min, these rises were more pronounced in the soluble portion of the enzyme than in total enzyme preparation, i.e., that containing mainly membrane-bound AChE. Moreover, from the 2nd DFP administration on, there was a persistent increase of medium-molecular-weight forms both in soluble and in total AChE. The results suggest an important role of the soluble portion of AChE and of medium forms in the process of recovery of enzymatic activity.

Chronic toxicity of technical fenitrothion in male rats

Weanling male Wistar rats were given 0.5, 1.0, 5.0 or 10.0 mg/kg body wt of technical fenitrothion ( O,O-dimethyl O-(4-nitro- m-tolyl) phosphorothioate in peanut oil by daily oral gavage for 12 months. Each month, 10 vehicle-and 5 insecticide-treated rats from each group were killed, tissues (liver, kidney, brain, spinal cord, and sciatic nerve) were preserved for morphological examination and the effects of treatment were assessed by measuring changes in activities of blood plasma pseudocholinesterase, erythrocytic and brain acetylcholinesterase (AChE), and hepatic and renal nonspecific carboxylesterases (CE) as well as routine hematology and serum biochemistry. At 12 months, administration was stopped and the survivors were held for an additional 2 months to monitor the recovery from the biological effects of the treatment. No changes were observed in the routine hematological or serum biochemical parameters measured during the treatment period at any of the doses administered. The morphological changes observed could not be related either to the vehicle or to a dose-dependent effect of fenitrothion. A dose-dependent inhibition of the tissue esterases was observed. Little change was measured at 0.5 and 1.0 mg/kg/day. Marked reductions in hepatic CE, brain, and erythrocytic AChE activities were observed at doses of 5.0 and 10.0 mg/kg/day within 1 month of treatment and remained relatively constant for the duration of treatment. Within 1 month after treatment was stopped, tissue esterase levels were within normal limits with the exception of the brain AChE in rats given the two larger doses. Within 2 months after treatment was terminated, tissue esterase activities of all treated rats were comparable to control activities.

Effects of ether and pentobarbital anaesthesia on the activities of brain acetylcholinesterase and butyrylcholinesterase in young adult rats.

Journal of NeurochemistryVolume 20, Issue 5 p. 1503-1504 Effects of ether and pentobarbital anaesthesia on the activities of brain acetylcholinesterase and butyrylcholinesterase in young adult rats1 Antonia Vernadakis, Antonia Vernadakis Departments of Psychiatry and Pharmacology, University of Colorado School of Medicine, Denver, Co. 80220, U.S.A.Search for more papers by this authorC. O. Rutledge, C. O. Rutledge Departments of Psychiatry and Pharmacology, University of Colorado School of Medicine, Denver, Co. 80220, U.S.A.Search for more papers by this author Antonia Vernadakis, Antonia Vernadakis Departments of Psychiatry and Pharmacology, University of Colorado School of Medicine, Denver, Co. 80220, U.S.A.Search for more papers by this authorC. O. Rutledge, C. O. Rutledge Departments of Psychiatry and Pharmacology, University of Colorado School of Medicine, Denver, Co. 80220, U.S.A.Search for more papers by this author First published: May 1973 https://doi.org/10.1111/j.1471-4159.1973.tb00267.xCitations: 25 1 This work was supported by USPHS Research Grant NS 09100 and a Research Scientist Development Award MH 42479 to Dr. Vernadakis from the National Insitute of Mental Health, National Institutes of Health. ‡ The authors are grateful for the technical assistance of Miss Katrina Gilmer and Miss Amelia A. Marlowe. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume20, Issue5May 1973Pages 1503-1504 RelatedInformation

Cortical acetylcholinesterase and handedness in rats.

Nachweis, dass es beim Lernprozess zu einem Anstieg der Acetylcholinesterase im kortikalen Reprasentationsareal einer trainierten Extremitat der Ratte kommt.