Acetylcholinesterase In Serum Research Articles

Modification of Michael's method for determination of serum acetyl choline esterase activity.

1. Through the improvement of Michael's method to determine CHE activity, it became clear that non-enzymatic hydrolysis of acetylcholin in the incubation medium depends on pHi and pHf, and enzymatic hydrolysis depends on pHi. 2. Using corrections b and i, stable and precise values of CHE activity has been able to gained. Calculated corrections b and i are shown, former as correction of non-enzymatic hydrolysis, latter as one of enzymatic hydrolysis. 3. Titration of incubation medium with acetic acid brings us comparable values between the units of△pH/hr. and μ mole/ml/hr. or min.