Acetohydroxamic Acid Research Articles

Towards catch-up therapy: evaluation of nucleophilic active pharmaceutical ingredients for the treatment of percutaneous VX poisoning, in-vial and in-vitro studies

Dermal exposure to low volatility organophosphorus chemical warfare agents (OP CWA) poses a great risk to the exposed person. Due to their lipophilic nature, these compounds rapidly absorb into the skin, leading to the formation of a “dermal reservoir” from which they slowly enter the bloodstream causing prolonged intoxication. Traditionally, strategies to counter the toxicity of such substances consist of chemical decontamination/physical removal of the residual agent from the skin surface (preferably as soon as possible following the exposure) and administration of antidotes in the case of intoxication signs. Hence, these strategies are unable to counter a substantial amount of the agent, which accumulates inthe dermal reservoir. More than a decade ago, the concept of a “catch-up therapy” intended to neutralize the dermal reservoir was suggested. Herein, we describe examples of potential “catch-up therapy” lotions - vehicles designed to deliver small nucleophilic molecules into the skin and potentially decompose the remaining CWA before it reaches the blood stream. Eleven nucleophilic compounds, based on approved drugs, were initially screened. They were then tested in various binary solutions, for their detoxification efficacy and degradation ability towards lipophilic OP CWA models such as dibutylphosphofluoridate and o-nitro-phenyl diphenyl phosphate, as well as the nerve agent VX, by means of kinetic 31P NMR and UV–Vis spectroscopy. Of these, the potassium and diethyl ammonium salts of acetohydroxamic acid (AHAK and AHA DEA) in (DMSO/H2O 1:4) were found to be the most active nucleophiles, hydrolyzing VX in practical time scales (t1/2 = 5.28 and 6.78 min, respectively). The vehicle solution DMSO/H2O 1:4 promoted the penetration of substantial amounts of AHA K and AHA DEA through excised pig skin in in-vitro studies, suggesting that such formulations may serve as useful CWA nucleophilic scavengers for both on and within -skin detoxification. These findings may pave the way to a more efficacious treatment against low volatility OP CWA percutaneous poisoning.

Radiolytic degradation of sulphonated BTP and acetohydroxamic acid under EURO-GANEX process conditions

In this work, we present a study which tackles the stability of molecules involved in EURO-GANEX aqueous phase, the acetohydroxamic acid (AHA) and the sulphonated BTP (SO3-Ph-BTP), taking into account factors such as the known AHA hydrolysis, acidity of the media, and the radiolysis of AHA and SO3-Ph-BTP. To better understand the system, the radiolysis of AHA and SO3-Ph-BTP in HNO3 has been studied first separately, at low doses (up to 50 kGy) compared with those expected for the recycled organic phase. The concentration after exposure to gamma radiation of AHA and its hydrolysis products, as well as of SO3-Ph-BTP, was measured for the first time by using Quantitative Raman Spectroscopy (QRS), showing that this technique can be used as a reliable tool for assessing the stability of these systems. In addition, the proton concentration and extraction efficiency of each assay were monitored and assessed. Our study extends the knowledge about the resistance to radiation of extractants involved in the EURO-GANEX aqueous phase, showing greater robustness when both molecules are irradiated together, and contributes not only to design reliable simulation strategies of the long-term EURO-GANEX process performance, but also to develop analysing tools for further processes implementations.

Application of Microwave Radiation to Produce Uranium Dioxide Powder from Its Trioxide

The possibility of producing UO2 powder from UO3 using microwave radiation (power 300 W) in the presence of organic compounds with amino groups: carbohydrazide (CH), acetohydroxamic (AHA), and aminoacetic (glycine) acids has been investigated. It was found that in these processes in an oxygen-free atmosphere, UO2 is produced from UO3, and in an air, U3O8. It was shown that under the action of microwave radiation in the presence of CH and AHA, powdered UO2 are synthesized from UO3. The physical properties of the powders obtained (bulk density with tapping 2.6–2.7 g/cm3, specific surface area up to 3.2 m2/g, moisture content less than 0.1 wt %) meet the requirements for ceramic-quality powders in the production of fuel pellets.

Effect of acetohydroxamic acid on the extraction of plutonium by dilute tri-n-butyl phosphate/n-dodecane in the presence of a high concentration of Al(NO3)3

Effect of acetohydroxamic acid on the extraction of plutonium by dilute tri-n-butyl phosphate/n-dodecane in the presence of a high concentration of Al(NO3)3

Insights into the selective adsorption mechanism of a multifunctional thioether-containing hydroxamic acid on separation of wolframite from fluorite

Both thioether and hydroxamate groups are excellent iron ligands. Herein, to improve the properties of hydroxamic acid and the recovery of tungsten-minerals, a surfactant containing thioether and hydroxamate groups, 2-(benzylthio)-acetohydroxamic acid (BTHA), was introduced to separate wolframite from fluorite. The properties, predicted by Density Functional Theory (DFT) calculations and Molecular Dynamics (MD), indicated that BTHA, as a multifunctional surfactant (i.e., thioether and hydroxamate groups), could enhance its hydrophobicity and selectively adsorb on the desired mineral surfaces. The experimental results of micro-flotation and contact angles confirmed BTHA had a strong collecting ability and good selectivity toward wolframite versus fluorite. The adsorption mechanism, surveyed through zeta potential, solution chemical analysis and XPS, demonstrated only electrostatic adsorption on fluorite surfaces, while chemisorption and hydrogen bonding would be produced on wolframite surfaces. The thioether and hydroxamate groups could co-absorb onto wolframite's surfaces to form FeS bonds and five-membered hydroxamate-(O, O)-Fe rings.

Countercurrent Actinide Lanthanide Separation Process (ALSEP) Demonstration Test with a Simulated PUREX Raffinate in Centrifugal Contactors on the Laboratory Scale

An Actinide Lanthanide Separation Process (ALSEP) for the separation of trivalent actinides (An(III)) from simulated raffinate solution was successfully demonstrated using a 32-stage 1 cm annular centrifugal contactor setup. The ALSEP solvent was composed of a mixture of 2-ethylhexylphosphonic acid mono-2-ethylhexyl ester (HEH[EHP]) and N,N,N′,N′-tetra-(2-ethylhexyl)-diglycolamide (T2EHDGA) in n-dodecane. Flowsheet calculations and evaluation of the results were done using the Argonne’s Model for Universal Solvent Extraction (AMUSE) code using single-stage distribution data. The co-extraction of Zr(IV) and Pd(II) was prevented using CDTA (trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid) as a masking agent in the feed. For the scrubbing of co-extracted Mo; citrate-buffered acetohydroxamic acid was used. The separation of An(III) from the trivalent lanthanides (Ln(III)) was achieved using citrate-buffered diethylene-triamine-N,N,N′,N″,N″-pentaacetic acid (DTPA), and Ln(III) were efficiently back extracted using N,N,N′,N′-tetraethyl-diglycolamide (TEDGA). A clean An(III) product was obtained with a recovery of 95% americium and curium. The Ln(III) were efficiently stripped; but the Ln(III) product contained 5% of the co-stripped An(III). The carryover of Am and Cm into the Ln(III) product is attributed to too few actinide stripping stages, which was constrained by the number of centrifugal contactors available. Improved separation would be achieved by increasing the number of An strip stages. The heavier lanthanides (Pr, Nd, Sm, Eu, and Gd) and yttrium were mainly routed to the Ln product, whereas the lighter lanthanides (La and Ce) were mostly routed to the raffinate.

Synthesis, Crystal Structures and Catalytic Property of Oxidovanadium(V) Complexes with N’-(4-Oxopentan-2-ylidene)nicotinohydrazide and 4-Bromo-N’-(4-oxopentan

A pair of structurally similar oxidovanadium(V) complexes with the general formula [VOLL’], with the hydrazone compounds N' -(4-oxopentan-2-ylidene)nicotinohydrazide (H 2 L 1 ) and 4-bromo- N' -(4-oxopentan-2-ylidene)benzohydrazide (H 2 L 2 ), and the acetohydroxamic acid (HL’) as ligands, have been synthesized and structurally characterized by physico-chemical methods and single crystal X-ray determination. Single crystal X-ray analysis indicates that the V atoms in the complexes are in octahedral coordination, with the ONO donor atoms of the hydrazone ligands, and the OO donor atoms of the acetohydroxamate ligands, as well as an oxido O atom. The complexes showed good property for the catalytic epoxidation of styrene.

Re: Inhibition of Urease Activity by Different Compounds Provides Insight into the Modulation and Association of Bacterial Nickel Import and Ureolysis

No AccessJournal of UrologyUrological Survey1 Dec 2020Re: Inhibition of Urease Activity by Different Compounds Provides Insight into the Modulation and Association of Bacterial Nickel Import and Ureolysis Dean G. AssimosMD Dean G. AssimosDean G. Assimos More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001280.02AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Re: Inhibition of Urease Activity by Different Compounds Provides Insight into the Modulation and Association of Bacterial Nickel Import and Ureolysis." The Journal of Urology, 204(6), p. 1375 Suggested Reading : Evaluation of biofilm induced urinary infection stone formation in a novel laboratory model system. J Urol 2018; 199: 178. Link, Google Scholar : Long-term studies of urease-induced crystallization in human urine. J Urol 1994; 152: 208. Link, Google Scholar : Infection stones. J Urol 1989; 141: 753. Link, Google Scholar : A randomized trial of acetohydroxamic acid for the treatment and prevention of infection-induced urinary stones in spinal cord injury patients. J Urol 1988; 140: 318. Link, Google Scholar © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 204Issue 6December 2020Page: 1375-1375 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dean G. Assimos More articles by this author Expand All Advertisement PDF DownloadLoading ...

Rate determining step of reduction Np(VI) by organic reagents from 30%TBP/Kerosene to nitrate solution

Abstract A series of single stage trials were conducted using six reducing reagents to investigate the rate determining step during Np(VI) reduction back-extraction from 30%TBP/Kerosene to HNO3 solution under the same conditions. The six reducing reagents included: acetohydroxamic acid, N,N-dimethylhydroxylamine, N,N-diethylhydroxylamine, 2-hydroxyethylhydrazine, methylhydrazine and N,N-dimethylhydrazine. Moreover, the back-extraction of Np(V) from 30%TBP/Kerosene to HNO3 solution without reducing reagent was conducted with the same concentrations of Np and HNO3. The experimental results indicate the rate of Np(VI) reduction in the two-phase system is determined mainly by the rate of Np(V) mass transfer from 30%TBP/Kerosene to HNO3 solution, whose mass transfer kinetics is far slower than previously estimated. The rate determining step is a slow chemical reaction as: NpO2NO3·TBP = NpO2 + + NO3 − + TBP.

Untargeted GC-MS investigation of serum metabolomics of coronary artery disease patients.

Recent advances in metabolomics provide tools to investigate human metabolome in order to establish new parameters to study different approaches towards diagnostics, diseases and their treatment. The present study focused on the untargeted identification of metabolites in serum of patients with coronary artery disease who were under treatment at the time of sample collection. AUCs (Area Under the Curves) from different peaks were considered for the analysis and comparison purposes. The metabolome was studied using GC–MS (Gas Chromatography Mass Spectrometry) and the metabolites were identified with NIST (The National Institute of Standards and Technology) and Wiley library matches. A total of 17 metabolites were identified and focused on to compare with the metabolome of healthy individuals. T test analysis found significant differences in alanine, malonic acid, ribitol, D-glucose, mannose (P < 0.001), acetohydroxamic acid, N-carboxyglycine, and aminobutyrate (P < 0.05). Principal Component Analysis of serum metabolites data found three components out of 17 metabolites; RC1 (Acetohydroxamic acid, alanine, D-glucose, malonic acid, mannose, N-carboxy glycine and ribitol), RC2 (Heptadecanoic acid, hexadecanoic acid, octadecanoic acid and Trans-9-octadecanoic acid), RC3 (Aminobutyrate, D-sorbit, gamma lactone, valine, benzene propanoic acid and lactic acid). No correlation was found among the components.

Synthesis and Structure-Activity Relationship Studies of N-monosubstituted Aroylthioureas as Urease Inhibitors.

Thiourea is a classical urease inhibitor which is usually used as a positive control, and many N,N'-disubstituted thioureas have been determined as urease inhibitors. However, due to steric hindrance, N,N'-disubstituted thiourea motif could not bind urease as thiourea. On the contrary, N-monosubstituted thiourea with a tiny thiourea motif could theoretically bind into the active pocket as thiourea. A series of N-monosubstituted aroylthioureas were designed and synthesized for evaluation as urease inhibitors. Urease inhibition was determined by the indophenol method and IC50 values were calculated using computerized linear regression analysis of quantal log dose-probit functions. The kinetic parameters were estimated via surface plasmon resonance (SPR) and by nonlinear regression analysis based on the mixed type inhibition model derived from Michaelis-Menten kinetics. Compounds b2, b11, and b19 reversibly inhibited urease with a mixed mechanism, and showed excellent potency against both cell-free urease and urease in the intact cell, with IC50 values being 90- to 450-fold and 5- to 50-fold lower than the positive control acetohydroxamic acid, respectively. The most potent compound b11 showed an IC50 value of 0.060 ± 0.004μM against cell-free urease, which bound to urea binding site with a very low KD value (0.420±0.003nM) and a very long residence time (6.7 min). Compound b11 was also demonstrated to have very low cytotoxicity to mammalian cells. The results revealed that N-monosubstituted aroylthioureas bound to the active site of urease as expected, and represent a new class of urease inhibitors for the development of potential therapeutics against infections caused by urease-containing pathogens.

Siderophore‐Assisted Dissolution of Iron(III) Hydroxide Oxides from Iron‐Rich Fossil Matrices

Current paleontological techniques to separate vertebrate fossils from encasing iron-rich cements by chemical means are limited by the low solubility of common iron(III) hydroxide oxides such as hematite and goethite. This study examines novel geochemical extractions capable of selectively dissolving iron(III) hydroxide oxides, in aqueous solutions of pH 9-11, without damaging fossilised bones or teeth (hydroxidecarbonate-apatite). This involves the siderophore ligands pyridoxal isonicotinoyl hydrazone (PIH), salicylaldehyde isonicotinoyl hydrazone (SIH), and acetohydroxamic acid (aHA), whose coordination complexes with iron(III) show exceptionally high formation stability constants. The methods have been tested on natural hematite and fossil containing samples from the Riversleigh World Heritage Area in Australia. Both 0.01 mol dm-3 aHA and 0.001 mol dm-3 PIH at pH 9.7 were able to dissolve over 0.1 mmol dm-3 of the goethite coating bone fragments.

Molecular Characterization of the Purine Degradation Pathway Genes ALA1 and URE1 of the Maize Anthracnose Fungus Colletotrichum graminicola Identified Urease as a Novel Target for Plant Disease Control.

Fungal pathogenicity is governed by environmental factors, with nitrogen playing a key role in triggering pathogenic development. Spores germinating on the plant cuticle are exposed to a nitrogen-free environment, and reprograming of nitrogen metabolism is required for bridging the time needed to gain access to the nitrogen sources of the host. Although degradation of endogenous purine bases efficiently generates ammonium and may allow the fungus to bridge the preinvasion nitrogen gap, the roles of the purine degradation pathway and of the key genes encoding allantoicase and urease are largely unknown in plant pathogenic fungi. To investigate the roles of the allantoicase and urease genes ALA1 and URE1 of the maize anthracnose fungus Colletotrichum graminicola in pathogenic development, we generated ALA1:eGFP and URE1:eGFP fusion strains as well as allantoicase- and urease-deficient mutants. Virulence assays, live cell, and differential interference contrast imaging, chemical complementation and employment of a urease inhibitor showed that the purine degradation genes ALA1 and URE1 are required for bridging nitrogen deficiency at early phases of the infection process and for full virulence. Application of the urease inhibitor acetohydroxamic acid did not only protect maize from C. graminicola infection, but also interfered with the infection process of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici, the maize and broad bean rusts Puccinia sorghi and Uromyces viciae-fabae, and the potato late blight pathogen Phytophthora infestans. Our data strongly suggest that inhibition of the purine degradation pathway might represent a novel approach to control plant pathogenic fungi and oomycetes.

Experimental and computational studies on hydroxamic acids as environmental friendly chelating corrosion inhibitors for mild steel in aqueous acidic medium

In the present study, three hydroxamic acids (HAs) namely acetohydroxamic acid (AHA), benzohydroxamic acid (BHA) and oxalohydroxamic acid (OHA) were synthesized, characterized and used as inhibitors for mild steel corrosion in1M HCl using chemical, electrochemical, surface and computational methods. Results of the studies show that the HAs act as effective corrosion inhibitors and their inhibition efficiencies follow the order: OHA (96.37%) > BHA (95.69%) > AHA (93.29%). EIS study showed that studied HAs act as interface type inhibitors. Polarization study revealed that HAs demonstrate mixed-type corrosion inhibitors characteristics and adsorb on the active sites of metallic surface. . Adsorption of HAs on metal-1 M HCl interfaces followed the Langmuir adsorption isotherm model. Surface morphological analyses of inhibited and uninhibited metallic surface were carried out using SEM-EDX and XRD methods. DFT analyses showed that studied compounds act as chelating type of ligands. Effect of Keto-enol tautomerism and different possible conformational isomers on metallic corrosion inhibition was demonstrated. The conformational isomers in which >C=O and –OH (hydroxyl) groups present in same side behave as chelating ligands and form relatively more stable complex than that of conformational isomers in which >C=O and –OH groups present in opposite side. Experimental and DFT studies complimented each other well.

MOLECULAR DYNAMICS STUDIES OF FULL HUMAN MATRIX METALLOPROTEINASE 9 LIGANDED WITH N-HYDROXY-2-[(4-PHENYLPHENYL) SULFONYL-PROPAN-2-YLOXYAMINO] ACETAMIDE

The research presented in this article aimed to provide a full quarternary structure of human matrix metalloproteinase 9 (MMP9) enzyme with a ligand in the catalytic site for structure-based virtual screening. The enzyme plays an important role in wound healing of diabetic foot ulcer. By employing the primary structure of the enzyme obtained from UniProt database (UniProt:P14780), the theoretical structure of full apoenzyme of the human MMP9 (PDB:1LKG), the crystal structures of the catalytic domain (PDB:4H3X) and the hemopexin domain (PDB:1ITV) of the human MMP9, homology modeling studies have been performed. The ligand N-2-(biphenyl-4-yl-sulfonyl)-N-2-(isopropyloxy)-acetohydroxamic acid (CC27) or N-hydroxy-2-[(4-phenylphenyl)sulfonyl-propan-2-yloxyamino]acetamide (IUPAC version) from PDB:4H3X was embedded in the catalytic site of the enzyme. The modeling made use of the modules of homology modeling in YASARA structure. Subsequently, molecular dynamics (MD) simulations in YASARA structure were performed to examine the stability of the enzyme. The homology model was found stable after 5.05 ns and the lowest energy of the model was found at the 6.40 ns of the MD production run. This lowest energy snapshot was then energetically minimized and analyzed for its applicability for virtual screening. This optimized model was then stored in Mendeley Data (DOI: 10.17632/4gsb4p75gz.1).

Enamine Barbiturates and Thiobarbiturates as a New Class of Bacterial Urease Inhibitors

Urease is a therapeutic target associated with several important diseases and health problems. Based on our previous work on the inhibition of glucosidase and other enzymes and exploiting the privileged structure assigned to the (thio)barbiturate (pyrimidine) scaffold, here we tested the capacity of two (thio)barbiturate-based compound collections to inhibit urease. Several compounds showed more activity than acetohydroxamic acid as a standard tested compound. In addition, by means of a conformational study and using the Density Functional Theory (DFT) method, we identified energetically low-lying conformers. Finally, we undertook a docking study to explore the binding mechanism of these new pyrimidine derivatives as urease inhibitors.

A novel surfactant 2-(benzylthio)-acetohydroxamic acid: Synthesis, flotation performance and adsorption mechanism to cassiterite, calcite and quartz

In order to improve the floatability of cassiterite, a novel surfactant 2-(benzylthio)-acetohydroxamic acid (BTHA) was synthesized and first used as flotation collector to selectively separate cassiterite from calcite and quartz. The flotation performance and adsorption mechanism of BTHA to cassiterite were studied by micro-flotation tests, zeta potential measurements, solution chemistry analysis, XPS and DFT calculations. The results of micro-flotation tests showed that BTHA possessed not only stronger collecting ability than either octanohydroxamic acid (OHA) or benzohydroxamic acid (BHA) under weak alkaline conditions, but also better selectivity to cassiterite against calcite and quartz. The results of zeta potential and solution chemistry analysis suggested that BTHA could produce co-adsorption between molecules and ions on the surface of cassiterite. The results of XPS spectra demonstrated that Sn-BTHA complexes were formed on cassiterite surfaces while no chemical bond or new chemical state emerged on calcite or quartz surfaces after BTHA treatment. DFT calculations provided further evidence that the BTHA interacted with cassiterite mainly through CO and OH in hydroxamate groups and S atoms in BTHA had certain electronic supply abilities which would promote the flotation of cassiterite.

Synthesis, Biological Evaluation and Molecular Docking of Deferasirox and Substituted 1,2,4-Triazole Derivatives as Novel Potent Urease Inhibitors: Proposing Repositioning Candidate.

A series of new deferasirox derivatives were synthesized through the reaction of monosubstituted hydrazides with 2-(2-hydroxyphenyl)-4H-benzo[e][1,3]oxazin-4-one. For the first time, deferasirox and some of its derivatives were evaluated for their in vitro inhibitory activity against Jack bean urease. The potencies of the members of this class of compounds are higher than that of acetohydroxamic acid. Two compounds, bearing tetrazole and hydrazine derivatives (bioisoester of carboxylate group), represented the most potent urease inhibitory activity with IC50 values of 1.268 and 3.254 μm, respectively. In silico docking studies were performed to delineate possible binding modes of the compounds with the enzyme, urease. Docking analysis suggests that the synthesized compounds were anchored well in the catalytic site and extending to the entrance of binding pocket and thus restrict the mobility of the flap by interacting with its crucial amino acid residues, CME592 and His593. The overall results of urease inhibition have shown that these target compounds can be further optimized and developed as a lead skeleton for the discovery of novel urease inhibitors.

Pantoprazole Derivatives: Synthesis, Urease Inhibition Assay and In Silico Molecular Modeling Studies

AbstractA number of pantoprazole derivatives were synthesized and screened for their urease inhibitory properties. Some of them showed potent inhibitions against jack bean urease. All compounds showed varying degree of IC50 in the range of 25.85 to 181 μMol as compared to standard acetohydroxamic acid (AHA) (100±2.02 μMol). Derivatives bearing 5‐aryl‐1,3,4‐oxadiazole ring substitutions (aryl= pyrazyl, pyridyl and phenyl) were found to be more potent inhibitors than AHA and pantoprazole. The most promising compound, 2‐((3,4‐dimethoxypyridin‐2‐yl)methylthio)‐5‐(pyrazin‐2‐yl)‐1,3,4‐oxadiazole 12, with IC50 value of 25.85±1.21 showed remarkable urease inhibition activity. In silico molecular modeling investigation performed to rationalize the possible binding interaction and ADME properties of compounds over the active site of urease enzyme. The induced fit docking study showed that compound 12 interacted with conserved residues His593 and Arg609 located at the mouth of the urease active site flap and are essential for enzyme catalytic activity. These target compounds could be further studied as a lead skeleton for discovery of novel urease inhibitors.

Acetohydroxamate-coordinated Oxovanadium(V) Complexes with Halide Containing Hydrazones: Synthesis, Characterization, X-ray Crystal Structures, and Antibacterial Activity

Two oxovanadium(V) complexes, [VO(L)(L1)] (I) and [VOLL2] (II), where L = acetohydroxamate, H2L1 = 2-bromo-N'-(2-hydroxybenzylidene)benzohydrazide, H2L2 = 2-chloro-N'-(2-hydroxybenzylidene)benzohydrazide,have been synthesized by reaction of VO(Acac)2 with acetohydroxamic acid and hydrazone ligands, and characterized by elemental analyses, IR, UV-Vis, 1H NMR, molar conductivity, and X-ray single crystal structural determination (CIF file CCDC nos. 1911887 (I), 1911888 (II)). The hydrazone ligands coordinate to the V atoms through phenolate oxygen, imino nitrogen, and enolate oxygen atoms. The acetohydroxamate ligand coordinate to the V atoms through the two oxygen atoms. The V atoms are in octahedral coordination with the sixth site coordinated by an oxo group. The antibacterial property of the complexes and the hydrazones against the bacteria B. subtilis, E. coli, P. putida, and S. aureus were studied. Both complexes exhibit remarkable antibacterial properties on B. subtilis and S. aureus comparable to Penicillin.