Mixture Of Acetic Acid Research Articles

Novel Bis-thiazoles with pyridine and 1,4-Dihydropyridine linkers as potential anti-Alzheimer agents

A series of bis-hydrazonothiazoles with pyridine and 1,4-dihydropyridine linkers was synthesized using 2,2′-[(2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-yl-1-ylidene)]bis(hydrazine-1-carbothioamide) and 2,2′-[(2,6-dimethylpyridine-3,5-diyl)bis(ethan-1-yl-1-ylidene)]bis(hydrazine-1-carbothioamide) as starting materials, which were reacted with 1-aryl-2-bromoethanone to form the desired bis-hydrazonothiazoles. Additionally, an alternative synthetic strategy involved the simple oxidation of 1,4-dihydropyridine linkers using a mixture of aqueous sodium nitrite and acetic acid to produce pyridine linkers. The synthesized compounds were subjected to molecular docking studies against the protein 1EVE (one of the AD bio-markers) to evaluate their 3-D orientation, flexibility, and conformational changes upon binding—key factors for optimizing drug design and therapeutic efficacy. The binding affinities of the synthesized compounds ranged from −1.14 to −11.64 kcal/mol, compared to -6.78 to -10.24 kcal/mol for the reference drugs. Statistical analysis revealed no significant difference in binding affinity between Donepezil and compounds 5c, 7a, 7b, and 8a, indicating similar therapeutic potential. Differences in binding affinities among the approved drugs underscored their distinct interaction profiles with the target protein. Molecular docking and SwissADME predictions helped prioritize molecules based on drug-likeness and pharmacokinetic profiles, identifying candidates for in-vivo validation and enhancing the drug development process.

Reaction of aroylpyruvic acids methyl esters with 4-aminobenzoic acid. Antioxidant activity of the obtained compounds

4-{[(2Z)-4-Aryl-2-hydroxy-4-oxobut-2-enoyl]amino}benzoic acids were synthesized by reacting methyl esters of aroylpyruvic acids with 4-aminobenzoic (p-aminobenzoic) acid in glacial acetic acid in the presence of anhydrous sodium acetate. 4-{[(2Z)-4-Aryl-1-methoxy-1,4-dioxobut-2-en-2-yl)]amino}benzoic acids were synthesized by reacting the above reagents in a mixture of glacial acetic acid–ethanol (1:1) without adding anhydrous sodium acetate. Structure of the obtained compounds was confirmed by 1H and 13C{1H} NMR and IR spectroscopy. The antioxidant activity of the synthesized compounds was studied.

Obtaining Lignin from Nutshells under Mild Extraction Conditions and Its Use as a Biostimulant in Tomato Seedlings

Biostimulants are an important alternative to improve and promote higher efficiency in cropping systems. Although the biostimulant industry has been developing for several years, there are still areas of opportunity for new sources of biostimulants as well as new ecofriendly extraction techniques that allow for a circular economy and the reuse of waste. Lignin is a heteropolymer that constitutes about 40% of the plant cell wall. A great source of lignin is agrowastes, giving it added value. Recently, its use has been tested in agronomy as a carrier of nutrients and pesticides. Walnuts are produced on a large scale in Northern Mexico, and the shell represents between 15 and 40% of its total weight. However, to obtain this biopolymer, to date, non-environmentally friendly techniques have been used; for this reason, it is necessary to find extraction alternatives to make this proposal sustainable. In this work, the obtaining and characterization of lignin through mild extraction conditions from nutshells and its evaluation as a biostimulant on the growth of tomato seedlings are reported. Lignin was extracted by hydrolysis with a mixture of acetic acid and distilled water (65:35 v/v). The results showed that it was possible to obtain 15% (w/w) lignin using mild solvents, evidenced by thermogravimetric analysis (TGA), proton magnetic nuclear resonance (H-RMN), and infrared (IR). Subsequently, lignin solutions were prepared at different concentrations, 0, 10, 50, and 100 ppm, and applied via foliar weekly to tomato seedlings. A greater fresh weight of the stem was found with 10 and 50 ppm, and the height and the fresh biomass increased with the three concentrations (10, 50, and 100 ppm), concluding that lignin extracted from nutshells using mild conditions can act as a plant biostimulant.

Adsorption Equilibrium, Kinetics, and Column Breakthrough Data for Aqueous Solutions of Binary-Acid and Ternary-Acid Mixtures of Acetic Acid, Butyric Acid, and Lactic Acid on IRN-78 Ion-Exchange Resin at Initial pH Levels of ∼3–7 and at 25–55 °C

Adsorption Equilibrium, Kinetics, and Column Breakthrough Data for Aqueous Solutions of Binary-Acid and Ternary-Acid Mixtures of Acetic Acid, Butyric Acid, and Lactic Acid on IRN-78 Ion-Exchange Resin at Initial pH Levels of ∼3–7 and at 25–55 °C

C–H Activation: A Versatile Tool for the Synthesis of Niclosamide and Its Derivatives

AbstractA novel strategy has been developed for the direct and regioselective ortho-acetoxylation of N-(2-benzoylphenyl)benzamides through C–H activation using a catalytic amount of Pd(OAc)2 (5 mol%) and a stoichiometric amount of PhI(OAc)2 in a mixture of acetic anhydride and acetic acid. By using this protocol, a new series of niclosamide derivatives was produced in good yields. This is the first report on the synthesis of niclosamide and its derivatives by means of C–H functionalization. This newly developed method offers several advantages such as high regioselectivity, operational simplicity, and good to excellent yields. It provides a short three-step process for the synthesis of niclosamide involving acid–amine coupling, ortho-acetoxylation through C–H activation, and deacylation.

Photopolymerization of Imprinted Polymer with Dummy Template for the Recognition of Hydroquinone in Aqueous Medium

This study' purposes are to synthesize molecularly imprinted polymer (MIP) with hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) using p-xylene under ultraviolet curing at 405 nm for the recognition of hydroquinone (HQ) in aqueous medium. The template was extracted from the polymer with a mixture of methanol and acetic acid (9:1) by volume (v/v). The Fourier transform infrared (FTIR) spectrum of MIP (after wash) showed the absence of peak at the range of 840–860 cm−1, which represented the stretching outside the aromatic plane C–H at the para position (p-xylene). Field emission scanning electron microscope (FESEM) micrograph showed that the MIP had cavities compared to non-imprinted polymer (NIP). The MIP (MIP-Pxy) with ratio (monomer:crosslinker) 0.25 and 1.00% template gave the highest uptake of hydroquinone (HQ) in aqueous solution, which implied more specific recognition (highest KD value). The rebinding of HQ onto MIP-Pxy was best described by both isotherm (Langmuir and Freundlich) and kinetic model (pseudo-first and -second). The MIP was successfully synthesized using p-xylene, able to recognize HQ and was very selective to p-CP. Implication of the study, the synthesized MIP can be used for recognition and sensing materials for HQ and any similar molecules.

Electrochemical Synthesis of Isoquinoline Sulfone Compounds and Study of their Inhibition of Acetolactate Synthase Enzyme Activity

AbstractBoth of sulfone compounds and isoqunoline derivatives are important compounds. In this paper, Isoquinoline C1 and C3 Sulfone compounds have been synthesized by electrochemical means using isoquinoline N‐Oxides and some sodium benzenesulfinate as reactants, inexpensive and easily available sodium iodide as electrolytes, and a mixture of acetonitrile, water and acetic acid as reaction solvents. By using the method, a series of isoquinoline sulfone compounds have been synthesized in high yields. The reaction does not need oxidants and catalysts, and the reaction conditions are mild, environmentally friendly, efficient and suitable. In addition, we also carried out ALS enzyme inhibition experiments on the synthesized isoquinoline sulfone compounds, six of the isoquinoline sulfone compounds synthesized herein were found to inhibit ALS enzyme at a high rate, four of them inhibited ALS enzyme at a rate comparable to that of the commercialized herbicides Halosulfuron and Broadstrike, and the other two compounds also inhibited ALS enzyme at a rate higher than that of Halosulfuron and Broadstrike. Therefore, it is possible to find lead compounds with good herbicidal activity potential from these 6 compounds.

Strategies for regulating crystallinity and surface quality based on solvent multistage volatilization

In this paper, Bi2212 superconducting thin films were prepared with metal acetate as salt source and a mixture of acetic acid and N,N-dimethylformamide (DMF) as solvent. The volatilization process of the solvent was regulated by changing the ratio of the mixed solvent, which in turn achieved the regulation of the surface morphology and crystallinity of the Bi2212 superconducting films. The mechanism of the mixed solvents on the crystallinity and surface quality of Bi2212 superconducting films was systematically investigated. The results showed that the Bi2212 superconducting films were all (00l) epitaxial growth. The grain size of Bi2212 increased continuously with the increase of the proportion of acetic acid in the solvents. The grain size of the largest grain size was 43.8 nm when the proportion of acetic acid was 80 %. However, the high proportion of acetic acid in the mixed solvents reduced the surface quality of Bi2212 thin films. The minimum surface roughness was 15.0 nm at 40 % acetic acid. All the samples exhibited good superconducting properties. This work provides a research basis for the systematic preparation of multicomponent oxide thin films.

Unveiling enhanced sound absorption in coconut wood through hemicellulose and lignin modification

Coconut wood (Cocos nucifera L.) is lightweight and has variable quality, making it a potential candidate for manufacturing sound absorption boards. However, its sound absorption coefficient needs enhancement to optimize its effectiveness in this application. This study aims to enhance its sound absorption properties using eco-friendly hydrogen peroxide and acetic acid treatments. This treatment modified the carbohydrate polymers (hemicellulose and cellulose) and lignin structures in the wood cell wall. The novelty of this approach lies in using these chemicals to improve acoustic performance significantly. Coconut wood samples were treated with a 1:1 acetic acid and hydrogen peroxide mixture for 20, 40, 60, and 80 min. Characterization techniques such as FTIR, XPS, and XRD, and 3D optical profilometry analyzed changes in chemical functionalities, crystallinity, and surface roughness. Sound absorption coefficients were measured using the impedance tube method. Results showed a significant improvement in sound absorption for all treated samples, especially at 60 min. The treatment also enhanced surface roughness, air permeability, porosity, and pore sizes, contributing to better sound absorption. This proposed treatment method addresses environmental consciousness and enhances the sustainable use and utilization of coconut wood.

تخليق وتحديد الطور الصلب البوليمري المطبوع بايون الكالسيوم في السيرم وتطبيقها في عينات سيرم

This study was aimed to synthesis new molecular imprinted polymers from different monomers which useful for determination of calcium ion in different serum samples. Calcium ion play an important role in blood clotting and bone mineralization. In plasma, 40 percent of circulating calcium is bound to proteins, 10 percent is in the form of inorganic complexes, and 50 percent is present as free (ionized) calcium. In this study, Blood samples were taken from patients with type 2 diabetes from Kadhimiya Hospital in order to determine the concentration of calcium ions in the blood, enter it into the column, and then calculate the final concentration in order to know the amount of dose taken by the patient. To acquire the highest adsorption capacity, molar ratios of the template, monomer, and cross-linking agent, as well as solvents and multiple monomers were investigated. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) were used to analyze the calcium ion polymer. The elution of calcium has a small effect on the surfaces of the three-dimensional network structure. Calcium (II) ions were successfully eluted using a mixture of methanol and acetic acid. The calcium absorption capacities were7.989 µmol/g and 8.250 µmol/g (Qmax), respectively. Solid-phase extraction (SPE) syringes packed with ionic imprinted polymers (IIPs) were used to selectively separate and preconcentration the calcium (II) ion from serum to determine the calcium ion by flame atomic absorption spectroscopy (FAAS). Through the results obtained and compared to the atomic absorption device, which is considered the most accurate and sensitive device for the elements, there is no difference in the results, and because the atomic absorption device test is expensive and needs electricity all the time, we can use the molecular printing technique to separate, assign, and concentrate the elements.

Functionalized ionic liquids as an efficient sorbent for solid-phase extraction of tetracyclines in bovine milk

In this research, a novel ionic liquid-based sorbent, N,N,N-trimethyl-3-(triethoxysilyl)propan-1-aminium bromide silica gel (SiO2-NH2), was successfully synthesized through chemical interaction. The synthesized adsorbent was first applied as a stationary phase for the preconcentration of tetracycline antibiotics (TCAs) at trace level from milk samples via solid-phase extraction. The structure of SiO2-NH2 was studied by Fourier-transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Energy-Dispersive X-ray Spectroscopy (EDX), and Scanning Electron Microscopy (SEM) to verify the successful synthesis based on the existence of ionic liquid on the surface of silica gel. The factors impacting a solid-phase extraction procedure were investigated, such as types of material, sample volume, breakthrough volume, and elution conditions. In addition, we also compared self-synthetic SPE cartridges with commercial cartridges with the assistance of LC-MS/MS. The SiO2-NH2 effectively retained and separated all three tetracycline antibiotics, including tetracycline (TC), oxytetracycline (OT), and chlortetracycline (CT), with a recovery efficiency of 99.4 % to 100 %. The maximum recovery was obtained when the analytes were eluted using a suitable mixture of acetonitrile, methanol, and acetic acid (2:2:1, v/v/v) for a volume of 1 mL. The enrichment factors of TC, OT, and CT were 110 mL, 150 mL, and 100 mL, respectively. The detection limits for TC, OT, and CT were 0.360, 0.279, and 0.390 mgL−1, corresponding with lower than 5 % relative standard deviation (RSD).

Solubility measurement, thermodynamic modeling, and molecular dynamic simulation of glycylglycine in five binary solvents

Glycylglycine (Gly–Gly) is a crucial biochemical reagent widely used in cosmetics, food additives, and medicine. Using the equilibrium method, the solubility data of Gly–Gly in four binary solvents (ethanol/isopropanol/ethanediol/acetone + water) were investigated at temperatures over a range of 288.15–328.15 K under 101.3 kPa, as well as in the binary solvent of acetic acid + water from 293.15 to 333.15 K. The solubility data were correlated by Apelblat-Jouyban Acree, Van’t Hoff-Jouyban Acree, and CNIBS/Redlich-Kister models. Additionally, the radial distribution function was obtained through molecular dynamic simulation. It presented the unusual dissolution behavior of Gly–Gly in acetic acid and water mixture. The apparent thermodynamic properties, including standard enthalpy, Gibbs free energy, and entropy change of dissolution, were obtained accordingly. This article could help the industrial crystallization of Gly–Gly.

Optimization of deep eutectic solvent based ultrasonic assisted microextraction for determination of zearalenone residues in foods

A sensitive and simple ultrasonic assisted microextraction procedure based on the use of green deep eutectic solvents (DESs) was designed for the extraction of zearalenone (ZEN) residues in grains, foods and milks. Optimization of key parameters and experimental modelling were achieved by Box-Behnken design (BBD) based on surface response methodology. DES-UA-ME procedure was based on the extraction of ZEN molecules into DES prepared from a mixture of phenyl acetic acid and trimethyl glycine in a 2:1 molar ratio at pH 3.8 under ultrasonication for 11 min at 33 °C. After the phase separation step, the DES-rich phase containing ZEN was placed in an UV-Vis spectrophotometer for quantitative analysis of ZEN at 260 nm. The analytical merits of the DES-UA-ME procedure were investigated using optimum conditions for both standard calibration solutions (SCS) and matrix-matched calibration solutions (MMCS). Linearity for the SCS and MMCS was 3–800 ng mL−1 (r2:0.995) and 5–450 ng mL−1 (r2:0.992), respectively. The limit of detection (LOD) and limit of quantification (LOQ) for SCS were 0.9 ng mL−1 and 3 ng mL−1, respectively, while the LOD and LOQ for MMCs were 1.6 ng mL−1 and 5 ng mL−1, respectively. Enrichment factors for SCS and MMCS was 157 and 139 for, respectively. Validation of the DES-UA-ME procedure was tested by analysis of two certified reference materials containing ZEN. There was no significant difference between the measured amounts and the reference amounts. In the final stage, the DES-UA-ME procedure was applied to selected samples separately for both SCS and MMCS.

Potensi Pewarna Alami untuk Pengembangan Kemajuan Teknologi Sel Surya Organik

Electrospinning is a modified technology to produce nano-sized fibers which can be used in making thin layers of semiconductor dye sensitized solar cells. Flourate – doped tin oxide (FTO) substrate was placed as a target for deposition of TiO2 nanofiber synthesized from TiO2 solution. The deposition process of a thin layer of TiO2 nanofiber was carried out for 15 minutes. The TiO2 solution synthesized is a mixture of acetic acid, ethanol, polyvinylpyrrolidone (PVP), and titanium tetra – isopropoxide (TTIP). Mixing the synthesis solution was carried out for 24 hours, to obtain a homogeneous TiO2 synthesis solution. The TiO2 nanofiber thin layer deposition process was applied under an electrospinning voltage of 25 kV, the distance of the FTO substrate as the collector layer to the solution syringe was 25 cm to avoid too large solution droplets. Before the deposition stage, the surface of the FTO substrate is first formed using the etching method by coating Zn and removing the conductive layer using HCl, so that a customized conductive layer is obtained. The annealing process of the deposited TiO2 nanofiber layer was carried out for 3 hours at a temperature of 450°C. The spin coating method was carried out to deposit a layer of Poly [2- methoxy- 5- (2- ethyl hexyloxy)- 1,4- phenylene venylene] (MEH-PPV). Coating variations are carried out with 4 different layer thicknesses. MEH – PPV was prepared by mixing chlorobenzene and stirring for 18 hours. Testing and characterization was carried out using current and voltage characterization (I – V meter), as well as image characterization using Scanning Electron Microscopy (SEM) as an analysis of the influence of layers on organic solar cells.

The influence of trihaloacetic acids on the hydrogen sorption properties of palladium nanoparticles-modified AB5 alloy in aprotic ionic liquid: Towards ionic liquid-based electrolytes for hydride batteries

Binary mixtures of acetic acid/trihaloacetic acids with aprotic ionic liquid – 1-ethyl-3-methylimidazolium methanesulfonate ([EMIM][MS]) are used as electrolytes for hydrogen electrosorption measurements. Preliminary studies are performed with palladium limited volume electrode to determine optimum concentration of the acid additives for hydrogen capacity and physicochemical properties of the electrolytes. The results obtained for trichloro- and tribromoacetic acid indicate that their decarboxylation occurs after mixing with [EMIM][MS] and dissolution of palladium is observed in contact with the decomposed electrolyte. Research on hydrogen capacity of palladium nanoparticles-modified AB5-type alloy is performed in mixtures of acetic acid and trifluoroacetic acid of optimum concentration. The best electrochemical performance is demonstrated by 2 M acetic acid/[EMIM][MS] mixture. The determined value of specific capacity of the investigated hydrogen absorbing material reaches 230 mAh g−1 and is close to that previously obtained from aqueous solutions of acids or bases.

Tuning bio-derived solvents for the rapid solubilization of astaxanthin-rich extracts from non-conventional bacterium Paracoccus carotinifaciens

Astaxanthin (AXT) is a ketocarotenoid widely used in food, feed, and pharmaceutical industries. Its biological sourcing is preferred over chemical methods due to higher physiological and commercial value. Paracoccus carotinifaciens, an aerobic marine Gram-negative bacterium, is known for producing a carotenoid mixture with AXT as the main component. This study explores the use of bio-based solvents, both pure and mixed, for extracting AXT-rich extracts (ARE). Using COSMO-SAC, a quantum chemistry-based thermodynamic model, we assessed the AXT-solvent affinity. The ethyl acetate: acetic acid mixture (EtOAc:AA) gave the best results, with 1.41 mg/mL of ARE. The solvent selection process was evaluated through the Eco Scale to compare with conventional methods. Next, optimization of extraction conditions resulted in 3.28 mg/mL of ARE at 78 °C, 10 min, and a solid-to-liquid ratio of 0.5 g/mL. Ultrasound-assisted extraction (UAE) was employed to tuning the mass-transfer process, leading to an increase of ARE (18.9 %) in reduced processing time. Concerning the stability of ARE in the EtOAc:AA mixture, the half-life (t1/2) reached 18 and 26 days under the light and dark conditions, respectively at 25 °C. In both light and dark conditions, positive enthalpy (ΔH) values revealed an endothermic process and both entropy (ΔS) and Gibbs free energy (ΔG) values suggest that the degradation of ARE is less disordered and non-spontaneous process. The solvent mixture was effectively reused for three cycles under optimally tuned conditions without a significant decline of ARE extraction efficiency.

Characterization of Flower Based Organic Dyes and Titanium Dioxide Thin Film for Dye Sensitized Solar Cell

In the present study, we described the organic dye extraction process from local flowers employing different methods and solvents (a mixture of acetic acid and ethanol, another is only acetic acid); especially we completed our research by exploring dye from red gerbera flower as it showed higher absorbance. Both dry and fresh petals of the flower were used for extracting dyes. The results revealed that the mixture-based dye extraction from dry red gerbera yielded a higher absorbance value of 4.579 a.u., whereas the acetic acid-based extraction from the same source exhibited a lower absorbance value of 2.778 a.u. The choice of solvent for dye extraction was found to significantly influence the absorption spectrum. Additionally, the Beer-Lambert law was employed to analyze the relationship between the absorbance and the concentration of the extracted dyes. For the electrode, a TiO2 film was created using the spin coating technique. From the XRD graph, we found the band gap energy of TiO2 which is 2.81eV (annealed at 450°C), the type of nanoparticle of the thin film. Also found a Crystallite size of 32.95nm and a specific Surface Area (SSA) of 43.048 m2.g−1, Microstrain (ε) of 5e-3 and Dislocation Density (δ) of 9.2e-4cm−2.

The Identification of Acetic Acid-Ethanol Mixture Using Gas Sensor Array and Ensemble Regression

Identification of acetic acid-ethanol mixtures using a commercial gas sensor array equipped with ensemble regression has been carried out. The gas sensor analysis was simple, rapid, and fast since it did not require any sample preparation. A quantitative analysis of the acetic acid-ethanol mixture was carried out to determine the sensitivity and selectivity of the sensor in distinguishing the concentration of the acetic acid and ethanol mixture. This study focuses on the coefficient of determination of 80% of the calibration data set and recovery of 20% of the testing data set. The models showed excellent performance,specifically, the Bagging and Random Forest r2 for the ethanol calibration data reached 0.91 and 0.94, respectively. The corresponding ethanol test recoveries were 99.95% and 97.84%, indicating the robustness of the model in accurately predicting ethanol concentration. Acetic acid test recoveries were 100.56% and 101.38% with r2 of 0.89 and 0.93 for Bagging and Random Forest regression, respectively. Hence, the commercial gas sensor array equipped with ensemble regression can be applied to the quantification of the acetic acid – ethanol mixture and demonstrate opportunities for the practical use of this gas sensor array in analyzing real samples, i.e. human breath or environmental monitoring samples.

Application of a Carbon Fiber Microelectrode as a Sensor for Apocynin Electroanalysis.

In this study, a carbon fiber microelectrode (CF) was applied for the investigation of the electrochemical behavior of the natural antioxidant, apocynin (APO). Given the limited solubility of APO in water, a mixture of anhydrous acetic acid (AcH) with 20%, v/v acetonitrile (AN) and 0.1 mol L-1 sodium acetate (AcNa) was used. The electrochemical properties of APO were examined through linear sweep voltammetry (LSV), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). The anodic oxidation of APO, which is the basis of the method used, proved to be diffusion-controlled and proceeded with a two-electron and one proton exchange. Both radicals and radical cations, arising from the first and second step of electrode reactions, respectively, underwent subsequent chemical transformations to yield more stable final products (EqCiEiCi mechanism). Using optimized DPV conditions, the anodic peak current of APO at a potential of 0.925 V vs. Ag/AgCl showed a good linear response within the concentration range of 2.7 × 10-6-2.6 × 10-4 mol L-1. The detection and quantification limits were determined as 8.9 × 10-7 and 2.7 × 10-6 mol L-1, respectively. The developed DPV method enabled the successful determination of APO in herbal extracts and in dietary supplements. It should be noted that this is the first method to be used for voltammetric determination of APO.

Synthesis of Bis-thiazoles Tethered 1,4-Dihydropyridine and Pyridine Linkers via Simple Oxidation and their Molecular Docking as VEGFR -TK Inhibitors.

In this study, a neoteric and expedient oxidation method is applied for a variety of Hantzsch 1,4-dihydropyridine derivatives such as 1,4-dihydro- 2,6-dimethyl-3,5-diacetylpyridine, 3,5-bis-hydrazono--2,6-dimethyl-1,4-dihydropyridine, and 3,5-bis-thiazoly-2,6-dimethyl-1,4-dihydro pyridine. This simple oxidation is based upon the in situ generation of nitrous acid from an aqueous sodium nitrite and acetic acid mixture and could be used to downgrade costs, sustain resources, and minimize chemical wastes. Also, a molecular modeling strategy was used to study the mechanism of action for various derivatives of bis-hydrazinylidene- thiazole as the protein Vascular Endothelial Growth Factor Receptor Tyrosine Kinase (VEGFR TK) inhibitor through evaluating their binding scores and modes compared with Sorafenib as a reference standard. The results revealed that the interaction of hydrazinylidene and thiazole as an anticancer Tyrosine Kinase inhibitor has been improved. Additionally, the compounds exhibiting the highest activity were assessed for their potential anticancer effects against HepG-2, MCF-7, and WI-38 cells, and the outcomes demonstrated encouraging activity against cancer.