Accurate Replication Research Articles

Mini-ridge filter designs for conformal FLASH proton therapy

The present study designed a mini-ridge filter capable of generating a 30 mm spread-out Bragg peak (SOBP) specific to the proton FLASH beamline for use in proton FLASH mouse studies. Proton FLASH therapy delivers ultra-high dose rates exceeding 40 Gy/s, necessitating instantaneous SOBP generation via static method. The TOol for PArticle Simulation (TOPAS) was used to model the FLASH beamline, which enabled accurate replication of beam characteristics identified from measurements, and ensured accurate dose calculations. Comparisons between simulated and measured peak widths and R80d values, accurate to within 1 mm, verified the accuracy and reliability of Monte Carlo code. Two mini-ridge filters of different materials were designed and optimized using MATLAB, simulated with TOPAS for SOBP verification, and fabricated using 3D-printing or computer numerical control manufacturing. The results indicated strong agreement between the simulated and measured SOBP widths for the polylactic acid mini-ridge filter but with discrepancies observed in the range and flatness measurements, which may be due to the use of 3D printing. By contrast, the aluminium alloy mini-ridge filter achieved excellent results, generating a 31.6 mm SOBP width with 0.89% flatness. Therefore, the present study validated the accuracy of our Monte Carlo code in simulating the FLASH beamline and the feasibility of our mini-ridge filters.

An interaction network in the polymerase active site is a prerequisite for Watson-Crick base pairing in Pol γ.

The replication accuracy of DNA polymerase gamma (Pol γ) is essential for mitochondrial genome integrity. Mutation of human Pol γ arginine-853 has been linked to neurological diseases. Although not a catalytic residue, Pol γ arginine-853 mutants are void of polymerase activity. To identify the structural basis for the disease, we determined a crystal structure of the Pol γ mutant ternary complex with correct incoming nucleotide 2'-deoxycytidine 5'-triphosphate (dCTP). Opposite to the wild type that undergoes open-to-closed conformational changes when bound to a correct nucleotide that is essential for forming a catalytically competent active site, the mutant complex failed to undergo the conformational change, and the dCTP did not base pair with its Watson-Crick complementary templating residue. Our studies revealed that arginine-853 coordinates an interaction network that aligns the 3'-end of primer and dCTP with the catalytic residues. Disruption of the network precludes the formation of Watson-Crick base pairing and closing of the active site, resulting in an inactive polymerase.

Organ-on-a-Chip Approach for Accelerating Blood-Brain Barrier Nanoshuttle Discovery.

Organ-on-a-chip, which recapitulates the dynamics of in vivo vasculature, has emerged as a promising platform for studying organ-specific vascular beds. However, its practical advantages in identifying vascular-targeted drug delivery systems (DDS) over traditional in vitro models remain underexplored. This study demonstrates the reliability and efficacy of the organ-on-a-chip in screening efficient DDS by comparing its performance with that of a conventional transwell, both designed to simulate the blood-brain barrier (BBB). The BBB nanoshuttles discovered through BBB Chip-based screening demonstrated superior functionality in vivo compared to those identified using transwell methods. This enhanced effectiveness is attributed to the BBB Chip's accurate replication of the structure and dynamics of the endothelial glycocalyx, a crucial protective layer within blood vessels, especially under shear stress. This capability of the BBB Chip has enabled the identification of molecular shuttles that efficiently exploit the endothelial glycocalyx, thereby enhancing transendothelial transport efficacy. Our findings suggest that organ-on-a-chip technology holds considerable promise for advancing research in vascular-targeted DDS due to its accurate simulation of molecular transport within endothelial systems.

Multi-objective optimization of hot embossing process for high-quality glass micro gratings

This study proposed a multi-objective optimization strategy to produce high-quality glass micro gratings. The applied methods include factor screening, surface response methodology (RSM), non-dominated sorting genetic algorithm (NSGA-II) and decision-making. Specifically, the significant factors were selected among several process parameters in hot embossing through screening experiments. Afterward, the regression models for the transferred ratio, uniformity of glass microstructures and warpage concerning those significant process parameters were established. Meanwhile, the significance of selected factors and their interactions was analyzed. Based on the regression models, the Pareto front was obtained by using NSGA-II program, where the top 10 solutions were applied in glass hot embossing. Finally, a decision-making strategy was employed to further choose a feasible solution that allows the fabrication of glass micro gratings with a high transfer ratio, high uniformity, low warpage, low surface roughness, and no defect. The experimental results indicated that embossing temperature has the most significant effect on the replication accuracy of glass micro gratings, followed by embossing force and embossing time. However, warpage was not significantly sensitive to any process parameters in this hot embossing process. The defect-free glass micro gratings could be eventually fabricated under the optimal process parameters, achieving a transfer ratio and uniformity of higher than 95 % and 83 %, respectively. Moreover, their Ra surface roughness and warpage can be lower than 13 nm and 1.5 μm. The satisfactory quality of embossed micro gratings revealed that the proposed multi-objective optimization strategy is feasible in glass hot embossing.

Microscopic analysis of mechanical anisotropy and damage evolution of 3D printed rock-like samples under uniaxial compressive tests

Three-dimensional printing (3DP) offers valuable insight into the characterization of natural rocks and the verification of theoretical models due to its high reproducibility and accurate replication of complex defects such as cracks and pores. In this study, 3DP gypsum samples with different printing directions were subjected to a series of uniaxial compression tests with in situ micro-computed tomography (micro-CT) scanning to quantitatively investigate their mechanical anisotropic properties and damage evolution characteristics. Based on the two-dimensional (2D) CT images obtained at different scanning steps, a novel void ratio variable was derived using the mean value and variance of CT intensity. Additionally, a constitutive model was formulated incorporating the proposed damage variable, utilizing the void ratio variable. The crack evolution and crack morphology of 3DP gypsum samples were obtained and analyzed using the 3D models reconstructed from the CT images. The results indicate that 3DP gypsum samples exhibit mechanical anisotropic characteristics similar to those found in naturally sedimentary rocks. The mechanical anisotropy is attributed to the bedding planes formed between adjacent layers and pillar-like structures along the printing direction formed by CaSO4·2H2O crystals of needle-like morphology. The mean gray intensity of the voids has a positive linear relationship with the threshold value, while the CT variance and void ratio have concave and convex relationships, respectively. The constitutive model can effectively match the stress–strain curves obtained from uniaxial compression experiments. This study provides comprehensive explanations of the failure modes and anisotropic mechanisms of 3DP gypsum samples, which is important for characterizing and understanding the failure mechanism and microstructural evolution of 3DP rocks when modeling natural rock behavior.

Optimizing Reverse-Engineered Finite Element Models for Accurate Predictions of Experimental Measurements

This study investigates the challenges of reverse engineering in finite element modelling of sheet metal forming, specifically for the Volvo XC90 front door inner component. Advanced models incorporating anisotropic behaviour of steel and non-linear friction are compared against actual real-world measurements. The methodology involves simplifying complex continuous parameters into more manageable representative data sets and assessing model accuracy under both uniform and varied blank holder force settings, guided by measured contact pressure distributions. Although the results indicate an improvement in accuracy, they underscore the need for additional methodological improvements and more accurate replication of tooling effects to enhance the fidelity and effectiveness of these models.

Transient acceleration waveform replication of electro-hydraulic systems using convex combined adaptive controller with input shaping technique

Electro-hydraulic systems are extensively utilized to generate desired acceleration waveforms to provide a vibration environment for testing the performance and reliability of objects in various industrial applications. However, as electro-hydraulic systems are often affected by some inevitable drawbacks resulted from hydraulic nonlinearities, unwanted dynamic variations and disturbances, the generated acceleration waveform is generally far behind the expectation. In this paper, a convex combined adaptive controller with input shaping technique is proposed for enhancing the transient acceleration waveform replication accuracy of electro-hydraulic systems. The proposed controller is comprised of a three variable controller at the bottom level, an input shaping technique controller at the middle level, and a convex combined adaptive controller at the upper level. The three variable controller is firstly utilized for the establishment of a fundamental closed-loop acceleration control system, and then the input shaping technique controller is constructed by introducing an offline designed inverse prefilter utilizing the multi-innovation recursive least squares algorithm and the zero magnitude error tracking algorithm. The convex combined adaptive controller at the upper level is comprised of two individual adaptive filters with high and low step sizes, which provides the merits of fast convergence rate and high tracking accuracy, and it is further exploited to address for system’s dynamic variations, model uncertainties and unexpected perturbations. Comparative experiments of the proposed controller with a manually generated random waveform and a recorded earthquake waveform as the testing inputs are conducted on a typical electro-hydraulic test bench, and the corresponding results demonstrate the feasibility and superiority of the proposed controller in improving the transient acceleration waveform replication performance of electro-hydraulic systems.

Surfaces of coastal biogenic structures: exploiting advanced digital design and fabrication strategies for the manufacturing of oyster reef and mussel bed surrogates

Coastal biogenic structures, formed by ecosystem engineering species, often feature rough surfaces characterized by intricate topographies and highly three-dimensional reliefs. Their surfaces are shaped by waves and tidal currents and reciprocally influence the ambient hydrodynamics, reflecting an equilibrium. Despite their significance, the impact of these surfaces on the ambient hydrodynamics remains underexplored due to limited knowledge of accurately replicating their complex topographies in experimental setups. The recent advent of advanced digital manufacturing presents an efficient means to manufacture highly complex, three-dimensional surrogate models for experimental modeling. This work explores the accurate replication of rough coastal biogenic structures for experimental modeling on the examples of an oyster reef and a mussel bed, utilizing a flexible design methodology and, for the first time, particle bed 3D printing with Selective Cement Activation (SCA) as a fabrication and manufacturing method. A workflow is proposed, which includes an iterative surrogate model development based on in-situ topographical features, requirements of the experimental setup, and parameters of the particle bed 3D printer with SCA. The results demonstrate the effectiveness of the methodology in achieving highly accurate surrogate surfaces of complex coastal biogenic structures by validation against a set of topographical features relevant to hydraulic roughness. Particle bed 3D printing with SCA proved to be a suitable method to manufacture complex surrogate surfaces for experimental modeling, offering advantages such as independence of production time from surface complexity. However, challenges persist in achieving exact comparability between the manufactured surrogate surface and the real coastal biogenic structures, particularly for surfaces with very high complexity. Nonetheless, the manufactured generic surrogate surfaces enable detailed investigations into the influence of complex coastal biogenic structures on the ambient hydrodynamics, thereby enhancing the understanding of the processes governing wave energy dissipation attenuation, turbulence production, and vertical mixing – critical for efficient application as a nature-based solution on coastal protection or restoration efforts.

Aphid alarm pheromone mimicry in transgenic Chrysanthemum morifolium: insights into the potential of (E)-β-farnesene for aphid resistance.

(E)-β-Farnesene (EBF) serves as the primary component of the alarm pheromone used by most aphid pest species. Pyrethrum (Tanacetum cinerariifolium) exhibits tissue-specific regulation of EBF accumulation and release, effectively mimicking the aphid alarm signal, deterring aphid attacks while attracting aphid predators. However, cultivated chrysanthemum (Chrysanthemum morifolium), a popular and economically significant flower, is highly vulnerable to aphid infestations. In this study, we investigated the high expression of the pyrethrum EBF synthase (TcEbFS) gene promoter in the flower head and stem, particularly in the parenchyma cells. Subsequently, we introduced the TcEbFS gene, under the control of its native promoter, into cultivated chrysanthemum. This genetic modification led to increased EBF accumulation in the flower stem and young flower bud, which are the most susceptible tissues to aphid attacks. Analysis revealed that aphids feeding on transgenic chrysanthemum exhibited prolonged probing times and extended salivation durations during the phloem phase, indicating that EBF in the cortex cells hindered their host-location behavior. Interestingly, the heightened emission of EBF was only observed in transgenic chrysanthemum flowers after mechanical damage. Furthermore, we explored the potential of this transgenic chrysanthemum for aphid resistance by comparing the spatial distribution and storage of terpene volatiles in different organs and tissues of pyrethrum and chrysanthemum. This study provides valuable insights into future trials aiming for a more accurate replication of alarm pheromone release in plants. It highlights the complexities of utilizing EBF for aphid resistance in cultivated chrysanthemum and calls for further investigations to enhance our understanding of this defense mechanism.

Surface modification of nickel-phosphorus plating by Ti ion implantation for chalcogenide glass lens molding

Nickel-phosphorus (Ni-P) plating has been developed as an excellent mold material in precision glass molding (PGM) for silicate optical lenses. However, the elemental diffusion and adhesion problems hinder the application of the Ni-P mold in the molding process for infrared chalcogenide glass (ChG) optics. In this study, Ti ion implantation and annealing were proposed for the surface modification of Ni-P plating. The modification mechanism of the Ti-Ni-P plating was studied, and the Ti ion distribution was simulated using the Monte Carlo method. Several characterization methods were conducted to analyze the surface composition of Ti-Ni-P plating. The mechanical properties of the plating were tested before and after implantation, proving that ion implantation improved the surface hardness of the plating. Finally, molding experiments were carried out using the Ti implanted flat and structured molds. The molded glass does not undergo interfacial reaction, and has high structural replication accuracy and higher infrared transmittance. It is validated that Ti ion implantation has a positive effect on the inhibition of the interfacial reaction in ChG molding.

Modeling vacancy-induced porosity in compositionally-graded complex alloys

A phase-field model of the void evolution is developed to investigate the vacancy-induced Kirkendall porosity within diffusion couples under isothermal annealing conditions. This model encapsulates a comprehensive computational framework for multicomponent diffusion, non-equilibrium vacancy diffusion, voids nucleation, and growth — integrating considerations for surface anisotropy and morphological instability. This novel thermodynamically consistent methodology combines the phase-field model with the vacancy diffusion model and uses thermodynamic and atomic mobility parameters defined by CALPHAD approach. The generalized nature of the method enables us to include both non-equilibrium and equilibrium vacancies, covering all cases of non-ideal/ideal sinks and sources for vacancies, whether at dislocations or void surfaces. Moreover, the model can deal with compositionally complex alloys such as Ni-based superalloys. Along with the full model, a fast variant is also suggested, providing long-time simulations at the experimentally relevant time and space scales valuable for both academic and industrial contexts. The model’s efficacy was demonstrated through precise simulation of voids evolution in a model AlCoCrTa/Ni diffusion couple, mirroring the experimental outcomes observed in the CMSX-10/Ni diffusion couple at 1525 K after 192 h of annealing. This encompassed accurate replication of diffusion profiles and tomographic analysis of voids. A very good agreement with the experimental data was observed, particularly in the distribution of pore volume-fractions and the delineation of void shapes.

Combining 3D printing and injection moulding for the fabrication of polymer micro-components with internal hollow features

There is a need to cost-effectively produce polymer components with meso/micro-scale internal geometries with high replication accuracy without the use of post-processing steps. A possible process chain to produce such polymer components with internal hollow features is by combining the 3D printing (3DP) and micro-injection moulding (MIM) processes. To date, no studies were carried out to explore the feasibility of such a process chain. Consequently, this experimental study investigated the use of the 3DP lost-cores that are over-moulded using the MIM process. The first step involved the production of lost-core from a soluble polymer material where three different materials were studied: two filament-based materials (Xioneer VXL130 and AquaSys180) and one resin-based material (IM-HDT-WS). The filament-based materials were printed on an Ultimaker S5 (filament fused fabrication) and the resin-based material was printed using an Asiga Max X27 (digital light processing). In the second step, the lost core was then over-moulded with polymethyl methacrylate (PMMA) using the MIM process. After demoulding, the internal core was then dissolved using the respective dissolution method of each material to achieve a part with meso/micro scale internal features. Investigations carried out at the different stages of the process chain revealed that the best dimensional accuracy was achieved when using the IM-HDT-WS material in the 3DP of the lost-cores and their subsequent over-moulding to form the case study part internal geometry. In particular, the dimensional analysis of the replicated IM-HDT-WS lost-core geometries onto the over-moulded PMMA revealed a difference of 0% in diameter and − 3.17% in bifurcation angle of the Y1.6 channel and a difference of + 4.88% in diameter and + 11.48% in bifurcation angle of the Y0.8 channels when compared to the respective 3DP core dimensional values prior to encapsulation. However, dissolution tests revealed that the filament-based material, the Xioneer VXL130, achieved a dissolution rate of 3.5 and 4.5 h for the Y1.6 and Y0.8 channel, respectively, which was marginally faster than that of the IM-HDT-WS.

Microbial induced wettability alteration with implications for Underground Hydrogen Storage

Characterization of the microbial activity impacts on transport and storage of hydrogen is a crucial aspect of successful Underground Hydrogen Storage (UHS). Microbes can use hydrogen for their metabolism, which can then lead to formation of biofilms. Biofilms can potentially alter the wettability of the system and, consequently, impact the flow dynamics and trapping mechanisms in the reservoir. In this study, we investigate the impact of microbial activity on wettability of the hydrogen/brine/rock system, using the captive-bubble cell experimental approach. Apparent contact angles are measured for bubbles of pure hydrogen in contact with a solid surface inside a cell filled with living brine which contains sulphate reducing microbes. To investigate the impact of surface roughness, two different solid samples are used: a “rough” Bentheimer Sandstone sample and a “smooth” pure Quartz sample. It is found that, in systems where buoyancy and interfacial forces are the main acting forces, the impact of biofilm formation on the apparent contact angle highly depends on the surface roughness. For the “rough” Bentheimer sandstone, the apparent contact angle was unchanged by biofilm formation, while for the smooth pure Quartz sample the apparent contact angle decreased significantly, making the system more water-wet. This decrease in apparent contact angle is in contrast with an earlier study present in the literature where a significant increase in contact angle due to microbial activity was reported. The wettability of the biofilm is mainly determined by the consistency of the Extracellular Polymeric Substances (EPS) which depends on the growth conditions in the system. Therefore, to determine the impact of microbial activity on the wettability during UHS will require accurate replication of the reservoir conditions including surface roughness, chemical composition of the brine, the microbial community, as well as temperature, pressure and pH-value conditions.

Beyond von Neumann Architecture: Brain‐Inspired Artificial Neuromorphic Devices and Integrated Computing

AbstractBrain‐inspired parallel computing is increasingly considered a solution to overcome memory bottlenecks, driven by the surge in data volume. Extensive research has focused on developing memristor arrays, energy‐efficient computing strategies, and varied operational mechanisms for synaptic devices to enable this. However, to realize truly biologically plausible neuromorphic computing, it is essential to consider temporal and spatial aspects of input signals, particularly for systems based on the leaky integrate‐and‐fire model. This review highlights the significance of neuromorphic computing and outlines the fundamental components of hardware‐based neural networks. Traditionally, neuromorphic computing has relied on two‐terminal devices such as artificial synapses. However, these suffer from significant drawbacks, such as current leakage and the lack of a third terminal for precise synaptic weight adjustment. As alternatives, three‐terminal synaptic devices, including memtransistors, ferroelectric, floating‐gate, and charge‐trapped synaptic devices, as well as optoelectronic options, are explored. For an accurate replication of biological neural networks, it is vital to integrate artificial neurons and synapses, implement neurobiological functions in hardware, and develop sensory neuromorphic computing systems. This study delves into the operational mechanisms of these artificial components and discusses the integration process necessary for realizing biologically plausible neuromorphic computing, paving the way for future brain‐inspired electronic systems.

Introducing a force-matched united atom force field to explore larger spatiotemporal domains in molecular dynamics simulations of bitumen

This paper presents a United Atom (UA) force field for simulating hydrocarbon molecules in bituminous materials, integrating explicit hydrogens into beads with their parent atom. This method simplifies all-atom molecular models, significantly accelerating Molecular Dynamics (MD) simulations of bitumen by 10 to 100 times. Key advantages include halving the particle count, eliminating complex hydrogen interactions, and decreasing the degrees of freedom of the molecules. Developed by mapping forces from an all-atom model to the centers of mass of UA model beads, the force field ensures accurate replication of energies, forces, and molecular conformations, mirroring properties like pressure and density. It features 17 bead types and 287 interaction types, encompassing various hydrocarbon molecules. The UA force field's stability, surpassing all-atom models, is a notable achievement. This stability, stemming from smoother potential energy surfaces, leads to consistent property measurements and improved stress tensor accuracy. It enables the extension of MD simulations to larger spatiotemporal scales, crucial for understanding complex phenomena such as phase separation in bituminous materials. This foundational work sets the stage for future developments, including refining parameters and introducing new bead types, to enhance the modeling capabilities of the force field, thereby advancing the application and understanding of bituminous materials.

Modeling the dynamic fracture of concrete — A robust, efficient, and accurate mesoscale description

Dynamic loading of concrete often leads to excessive cracking and fragmentation. Since these processes are massively influenced by the underlying heterogeneity of the material, discrete modeling of the lower-scale features strongly improves the simulation results. At the same time, the computational effort is greatly increased. We therefore propose here a mesomechanical simulation approach which is efficient in terms of model generation and computational efforts even for large 3d models. Furthermore, it is robust by limiting the application of computationally less efficient cohesive zone elements to the weak transition boundary between matrix and inclusions. Intermatrix failure is considered by a smeared crack approach and element removal after a certain crack opening has been reached. We apply an improved version of the widely used RHT concrete model, which is enhanced with a principal stress criterion. The resulting approach leads to an accurate replication of experiments in terms of crack propagation and stress wave induced dynamic fragmentation.

Numerical study of a fish swimming in hydrokinetic turbine wake

The environmental effects of hydrokinetic turbines are still under investigation, reflecting the emerging status of this technology. This study investigates the interaction between hydrokinetic rotor wakes and fish swimming, revealing insights into fish biomechanics in complex flows and assessing the environmental implications of marine energy solutions. We conducted numerical simulations with the URANS approach and k−ω−SST turbulence closure model to predict three-dimensional turbulent flow in the OpenFOAM software. The hydrokinetic rotor wake was simulated employing the actuator line method, providing a computationally efficient alternative to full geometry simulations. For accurate replication of the motion of a fish-like tuna (Thunnus atlanticus), dynamic adaptive mesh discretization was employed. The results offer a comparative analysis of fish swimming performance within the wake rotor, particularly when immersed in the tip blade vortex, contrasted with scenarios where fish swim in undisturbed flow conditions. The analysis encompasses three-dimensional wake structures, force generation, efficiency, and equilibrium states (balancing drag and thrust) across varying Swimming numbers (Sw). Key findings include the enhanced attachment of the leading-edge vortex due to the caudal fin’s interaction with the tip blade vortex, resulting in improved auto-propulsive force production; a reduced tail stride frequency observed in fish swimming downstream of the rotor to achieve longitudinal force balance compared to unperturbed flow; and transverse hydrodynamic forces pushing fish radially away from the wake’s influence zone, potentially mitigating the risk of collision with turbine blades.

Anatomically-Inspired Robotic Finger with SMA Tendon Actuation for Enhanced Biomimetic Functionality.

This research introduces an advanced robotic finger designed for future generalist robots, closely mimicking the natural structure of the human finger. The incorporation of rarely discussed anatomical structures, including tendon sheath, ligaments, and palmar plates, combined with the usage of anatomically proven 3D models of the finger, give rise to the highly accurate replication of human-like soft mechanical fingers. Benefiting from the accurate anatomy of muscle insertions with the utilization of Shape Memory Alloy (SMA) wires' muscle-like actuation properties, the bonding in-between the flexor tendons and extensor tendons allows for the realization of the central and lateral band of the finger anatomy. Evaluated using the computer vision method, the proposed robotic finger demonstrates a range of motion (ROM) equivalent to 113%, 87% and 88% of the human dynamic ROM for the DIP, PIP and MCP joints, respectively. The proposed finger possesses a soft nature when relaxed and becomes firm when activated, pioneering a new approach in biomimetic robot design and offering a unique contribution to the future of generalist humanoid robots.

Timeless-Tipin interactions with MCM and RPA mediate DNA replication stress response.

The accuracy of replication is one of the most important mechanisms ensuring the stability of the genome. The fork protection complex prevents premature replisome stalling and/or premature disassembly upon stress. Here, we characterize the Timeless-Tipin complex, a component of the fork protection complex. We used microscopy approaches, including colocalization analysis and proximity ligation assay, to investigate the spatial localization of the complex during ongoing replication in human cells. Taking advantage of the replication stress induction and the ensuing polymerase-helicase uncoupling, we characterized the Timeless-Tipin localization within the replisome. Replication stress was induced using hydroxyurea (HU) and aphidicolin (APH). While HU depletes the substrate for DNA synthesis, APH binds directly inside the catalytic pocket of DNA polymerase and inhibits its activity. Our data revealed that the Timeless-Tipin complex, independent of the stress, remains bound on chromatin upon stress induction and progresses together with the replicative helicase. This is accompanied by the spatial dissociation of the complex from the blocked replication machinery. Additionally, after stress induction, Timeless interaction with RPA, which continuously accumulates on ssDNA, was increased. Taken together, the Timeless-Tipin complex acts as a universal guardian of the mammalian replisome in an unperturbed S-phase progression as well as during replication stress.

Digital Protocol to Record Occlusal Analysis in Prosthodontics: A Pilot Study.

Background: Digital technologies enable the accurate replication of occlusion, which is pivotal for stability in maximum intercuspation and dynamic occlusion. CAD softwares generates standardized occlusal morphologies requiring significant adjustments. The consideration of individual mandibular movements during restoration leads to better functional integration. This pilot study evaluates the efficacy of a novel, fully digital protocol for occlusal analysis recording in prosthodontics. Methods: Patients needing single or multiple metal-free restorations were included. Teeth underwent horizontal finish line preparation, while restorations on implants were either directly screwed or used multi-unit abutments. A digital impression (Trios 3 Intraoral Scanner) captured the mouth's elements. Dynamic occlusion was recorded via Patient Specific Motion (PSM). After the placement and functionalization of temporary restorations, subsequent scans included various elements, and CAD software (Dental system) was used for the restoration design. Restorations were milled in monolithic zirconia, pressed from CAD/CAM-milled wax, and sintered. Results: An evaluation of 52 restorations in 37 patients indicated high accuracy in restorations manufactured via the fully digital workflow. Monolithic zirconia was predominantly used. Subtractive (17.3%) and additive (7.7%) occlusal adjustments were mainly chairside. Conclusion: This study underscores the efficacy of meticulous verification measures and a centric contact system in reducing the need for clinical occlusal refinements in prosthetic restorations.