Pigment Accumulation Research Articles

Transcriptomic study of the role of MeFtsZ2-1 in pigment accumulation in cassava leaves.

MeFtsZ2-1 is a key gene for plant plastid division, but the mechanism by which MeFtsZ2-1 affects pigment accumulation in cassava (Manihot esculenta Crantz) through plastids remains unclear. We found that MeFtsZ2-1 overexpression in cassava (OE) exhibited darker colors of leaves, with increased levels of anthocyanins and carotenoids. Further observation via Transmission Electron Microscopy (TEM) revealed no apparent defects in chloroplast structure but an increase in the number of plastoglobule in OE leaves. RNA-seq results showed 1582 differentially expressed genes (DEGs) in leaves of OE. KEGG pathway analysis indicated that these DEGs were enriched in pathways related to flavonoid, anthocyanin, and carotenoid biosynthesis. This study reveals the role of MeFtsZ2-1 in cassava pigment accumulation from a physiological and transcriptomic perspective, providing a theoretical basis for improving cassava quality.

Autophagy-regulated ethylene synthesis mediates fruit ripening by affecting the accumulation of lycopene, sugars and organic acids in tomato

Autophagy is a universal cellular process in eukaryotes that plays a critical role in plant growth and stress response. However, the role of autophagy in fruit ripening is largely unknown. Here, we demonstrated that most autophagy-related genes (ATGs) were up-regulated during tomato (Solanum lycopersicum L.) fruit ripening. By using mutants of different autophagy pathway genes (ATG6, ATG10, ATG18a), we revealed that the deficiency of autophagy delayed the ripening of fruit. Compared with wild-type (WT), the production of ethylene was significantly reduced and the accumulation of lycopene was delayed in atg mutants during fruit ripening. We also observed the contents of glucose and fructose were both significantly decreased in atg mutants compared with WT, while the content of organic acids showed the opposite trend. Additionally, the negative regulator of ethylene production, APETALA2a (AP2a), interacted with ATG8 through a specific ATG8-interacting motif (AIM) and could be degraded through the autophagy pathway. These results demonstrate that autophagy plays a critical role in fruit ripening by regulating ethylene production and the accumulation of pigments, sugars and organic acids in tomato.

Effect of Phosphorus on the Growth, Pigmentation and Lipid Accumulation in Microalgae Picochlorum sp.

Picochlorum sp. algae are recognized for their abundant bioactive compounds, which include valuable substances such as astaxanthin, carotenoids, fatty acids, and lutein. The significant total lipid content found in Picochlorum sp. algae presents diverse opportunities for utilization across the realms of bioenergy, food, and pharmaceuticals. phosphorus concentrations ranging from 0.02 g/L to 0.16 g/L, the growth of Picochlorum sp. did not exhibit any significant differences across these concentrations. However, the chlorophyll and carotenoid contents peaked in the control group and at phosphorus concentrations of 0.02 g/L and 0.04 g/L, respectively, after 12 days of cultivation. In contrast, the lipid content increased in both the control group and at a phosphorus concentration of 0.16 g/L after 9 days of cultivation.

Growth, Physiological, and Biochemical Responses of a Medicinal Plant Launaea sarmentosa to Salinity

Launaea sarmentosa is a valuable medicinal plant with adaptability in saline areas, but it is still unclear how it responds to salinity. For the first time, the present study examined the plant’s changes under different soil salinities generated by 50, 100, 200, and 400 mM NaCl in order to elucidate its responses in terms of growth, photosynthesis, water uptake, osmotic adjustment, ion homeostasis, and oxidative stress defense to salinity. The results showed that the plant’s growth was enhanced by 50 mM NaCl with an 18.07% increase in dry biomass compared to the control, whereas higher salinity levels reduced its growth with a 6.39–54.85% decrease in dry biomass. The plant’s growth response indicates that it had tolerance to salinity levels up to 400 mM NaCl. The accumulation of photosynthetic pigments, including chlorophylls (a + b) and carotenoids, was enhanced under salinity, except for a reduced accumulation under 400 mM NaCl. Relative water content decreased while proline content increased in the salt-stressed plants. Moreover, the salt-stressed plants reduced their K+ and NO3− content along with increases in Na+ and Cl− content. The high salt stress level also caused oxidative stress in the plants, which was revealed through the accumulation of malondialdehyde and hydrogen peroxide content. In addition, the salt-stressed plants had increased total phenolic content and the activities of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase. These physiological and biochemical changes suggest that L. sarmentosa evolved adaptive mechanisms in photosynthesis, osmotic adjustment, ion homeostasis, and antioxidant defense for growing under salt stress.

Regulatory T cells limit age-associated retinal inflammation and neurodegeneration

BackgroundAgeing is the principal risk factor for retinal degenerative diseases, which are the commonest cause of blindness in the developed countries. These conditions include age-related macular degeneration or diabetic retinopathy. Regulatory T cells play a vital role in immunoregulation of the nervous system by limiting inflammation and tissue damage in health and disease. Because the retina was long-considered an immunoprivileged site, the precise contribution of regulatory T cells in retinal homeostasis and in age-related retinal diseases remains unknown.MethodsRegulatory T cells were selectively depleted in both young (2–4 months) and aged (18–23 months) FoxP3-DTR mice. We evaluated neuroretinal degeneration, gliosis, subretinal space phagocyte infiltration, and retinal pigmented epithelium morphology through immunofluorescence analysis. Subsequently, aged Treg depleted animals underwent adoptive transfer of both young and aged regulatory T cells from wild-type mice, and the resulting impact on neurodegeneration was assessed. Statistical analyses employed included the U-Mann Whitney test, and for comparisons involving more than two groups, 1-way ANOVA analysis followed by Bonferroni’s post hoc test.ResultsOur study shows that regulatory T cell elimination leads to retinal pigment epithelium cell dysmorphology and accumulation of phagocytes in the subretinal space of young and aged mice. However, only aged mice experience retinal neurodegeneration and gliosis. Surprisingly, adoptive transfer of young but not aged regulatory T cells reverse these changes.ConclusionOur findings demonstrate an essential role for regulatory T cells in maintaining age retinal homeostasis and preventing age-related neurodegeneration. This previously undescribed role of regulatory T cells in limiting retinal inflammation, RPE/choroid epithelium damage and subsequently photoreceptor loss with age, opens novel avenues to explore regulatory T cell neuroprotective and anti-inflammatory properties as potential therapeutic approaches for age-related retinal diseases.

Aging and intraocular pressure homeostasis in mice.

Age and elevated intraocular pressure (IOP) are the two primary risk factors for glaucoma, an optic neuropathy that is the leading cause of irreversible blindness. In most people, IOP is tightly regulated over a lifetime by the conventional outflow tissues. However, the mechanistic contributions of age to conventional outflow dysregulation, elevated IOP and glaucoma are unknown. To address this gap in knowledge, we studied how age affects the morphology, biomechanical properties and function of conventional outflow tissues in C57BL/6 mice, which have an outflow system similar to humans. As reported in humans, we observed that IOP in mice was maintained within a tight range over their lifespan. Remarkably, despite a constellation of age-related changes to the conventional outflow tissues that would be expected to hinder aqueous drainage and impair homeostatic function (decreased cellularity, increased pigment accumulation, increased cellular senescence and increased stiffness), outflow facility, a measure of conventional outflow tissue fluid conductivity, was stable with age. We conclude that the murine conventional outflow system has significant functional reserve in healthy eyes. However, these age-related changes, when combined with other underlying factors, such as genetic susceptibility, are expected to increase risk for ocular hypertension and glaucoma.

Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat

Pigment accumulation is an important trait related to wheat domestication, but there remains a limited understanding of its molecular mechanism. The genetic control of the red glume trait by a dominant allele, Rg-B1, on 1BS was reported in the last century, but the underlying gene and its molecular basis remained elusive. Here, we identified TraesTSP1B01G005700 (G57) encoding an R2R3-MYB transcription factor (TF) as the candidate Rg-B1 gene controlling red glume color by a combination of genome-wide association study (GWAS), bulked segregant RNA-sequencing (BSR-Seq), map-based cloning, and RNA-seq. TheRg-B1locus had zero to five duplicate copies only one of whichhadhightranscriptional activity. Genetic evidence suggested that promoter sequence variation in G57 in the glumes leads to high expression of G57, resulting in the red glume phenotype. G57 could bind to the promoters of anthocyanin synthesis genes TaCHS, TaF3ʹH, and TaUFGT, activating their expression and contributing to anthocyanin accumulation in wheat glume. G57 also played a pivotal role in up-regulating expression of genes TaDREB1C and TaFLO2 associated with increased grain weight, thereby causing increased grain weight. Our research offers a better understanding of the molecular basis of red glume in bread wheat.

Seasonal dynamics of photosynthetic pigments (chlorophylls a, b and carotenoids) in woody plants of the Altai Botanical Garden

This article reflects the results of studies of photosynthetic pigments (chlorophylls a, b and carotenoids) in 14 species of deciduous plants (trees and shrubs) and one coniferous Juniperus sabina L. in the Altai Botanical Garden during two growing seasons. The following species have a high accumulation of pigments: Acer tataricum L., Juglans cinerea L., Tilia cordata L., Corylus avellana L., Cotoneaster lucidus Schlecht., Lonicera tatarica L. For the coniferous species Juniperus sabina L. low levels of chlorophylls a, b and carotenoids are characteristic. Berberis thunbergii DC is also distinguished by a low accumulation of photosynthetic pigments. Minor differences between the species were noted in the content of carotenoids, twelve species were included in the group with low and medium content of this pigment. There is no definite pattern in the accumulation of carotenoids, and the data on the types and number of yellow pigments do not differ significantly. As a result of the conducted studies, a correlation was revealed between the content of carotenoids and the content of chlorophyll a in most species. The data obtained indicate the presence of species specificity in the content of chlorophylls a, b and carotenoids. Also, the photosynthetic apparatus of woody plants is sensitive to light and water conditions

Phloretic acid requires the insulin/IGF-1 pathway and autophagy to enhance stress resistance and extend the lifespan of Caenorhabditis elegans.

Objective: In humans, aging is associated with increased susceptibility to most age-related diseases. Phloretic acid (PA), a naturally occurring compound found in Ginkgo biloba and Asparagus, exhibits has potential as an anti-aging agent and possesses antioxidant, anti-inflammatory, and immunomodulatory properties. This study aimed to investigate the effects of PA on longevity and stress resistance in Caenorhabditis elegans (C.elegans) and the mechanisms that underlie its effects. Methods: First, we examined the effects of PA on lifespan and healthspan assay, stress resistance and oxidative analysis, lipofuscin levels. Second, we examined the insulin/insulin-like pathway, mitochondria, autophagy-related proteins, and gene expression to explain the possible mechanism of PA prolonging lifespan. Results: Our findings demonstrated that PA dose-dependently extended the C.elegans lifespan, with 200μMPA showing the greatest effect and increased the C.elegans lifespan by approximately 16.7%. PA enhanced motility and the pharyngeal pumping rate in senescent C.elegans while reducing the accumulation of aging pigments. Further investigations revealed that daf-16, skn-1, and hsf-1 were required for mediating the lifespan extension effect of PA in C.elegans since its impact was suppressed in mutant strains lacking these genes. This suggests that PA activates these genes, leading to the upregulation of downstream genes involved in stress response and senescence regulation pathways. Furthermore, PA did not extend the lifespan of the RNAi atg-18 and RNAi bec-1 but it attenuated SQST-1 accumulation, augmented autophagosome expression, upregulated autophagy-related gene expression, and downregulated S6K protein levels. These findings suggest that the potential life-extending effect of PA also involves the modulation of the autophagy pathway. Conclusion: These findings results highlight the promising anti-aging effects of PA and warrant further investigation into its pharmacological mechanism and medicinal development prospects.

Peculiarities of biochemical and mineral composition of lettuce <i>Lactuca sativa</i> L. grown from seeds after long-term storage in the International Space Station

Seed storage of agricultural crops in the conditions of International Space Station (ISS) is an effective method of quick plant breeding aimed to enhance the adaptability, and improve the biologically active compound accumulation and crop yield. Biochemical and mineral composition (ICP-MS) of lettuce grown in greenhouse from seeds stored for one year at the ISS was assessed. High varietal differences in lettuce tolerance to the effect of micro-gravity and radiation were evaluated using the values of yield, biometrical parameters, antioxidant status and plant mineral composition. Among the cultivars studied, i.e. Petrovich, Synthesis, Moskovsky parnikovy, Picnic, Cavalier and Bouquet, only the cvs. Retrovich, Synthesis, Moskovsky parnikovy and Picnik retained seed viability. Significant yield increase was recorded for the cv. Moskovsky parnikovy, while the cvs. Petrovich, Synthesis and Picnic demonstrated a tendency to the yield decrease. Improvement of photosynthetic pigment accumulation due to seed space storage was recorded for the cv. Moskovsky parnikovy and, particularly, the latter cultivar differed from the other cvs. due to the significantly higher proline level and lower MDA levels in leaves. Though space stress did not cause any significant decrease in antioxidant accumulation of plants, the latter demonstrated valuable changes in mineral composition. Among the 23 elements tested, V, Se. Fe and Cr showed higher concentrations compared to those in the control plants. Positive correlations between Ca, Sr, Cd, Cp, Mg, Mo and Pb were recorded (r > 0.9; p < 0.001).

The RUBY reporter for visual selection in soybean genome editing.

Current systems to screen for transgenic soybeans (Glycine max) involve laborious molecular assays or the expression of fluorescent markers that are difficult to see in soybean plants. Therefore, a visual system for early screening of transgenic plants would increase the efficiency of crop improvement by genome editing. The RUBY reporter system, which consists of three genes encoding betalain biosynthetic enzymes, leading to the accumulation of purple pigment in transgenic tissue, has been employed in some plants and dikaryon fungi. Here, we assessed the RUBY reporter for visual verification during soybean transformation. We show that RUBY can be expressed in soybean, allowing for visual confirmation of transgenic events without the need for specialized equipment. Plants with visible accumulation of purple pigment in any tissue were successfully transformed, confirming the accuracy of the RUBY system as a visual indicator. We also assessed the genetic stability of the transgene across generations, which can be performed very early, using the cotyledons of the progeny. Transgene-free seedlings have a distinct green color, facilitating the selection of genome-edited but transgene-free soybean seedlings for harvest. Using the RUBY system, we quickly identified a transgene-free Gmwaxy mutant in the T1 generation. This system thus provides an efficient and convenient tool for soybean genome editing.

Seasonal dynamics of photosynthetic pigments (chlorophylls a, b and carotenoids) in woody plants of the Altai Botanical Garden

This article reflects the results of studies of photosynthetic pigments (chlorophylls a, b and carotenoids) in 14 species of deciduous plants (trees and shrubs) and one coniferous Juniperus sabina L. in the Altai Botanical Garden during two growing seasons. The following species have a high accumulation of pigments: Acer ta-taricum L., Juglans cinerea L., Tilia cordata L., Corylus avellana L., Cotoneaster lucidus Schlecht., Lonicera tatarica L. For the coniferous species Juniperus sabina L. low levels of chlorophylls a, b and carotenoids are characteristic. Berberis thunbergii DC is also distinguished by a low accumulation of photosynthetic pigments. Minor differences between the species were noted in the content of carotenoids, twelve species were included in the group with low and medium content of this pigment. There is no definite pattern in the accumulation of carotenoids, and the data on the types and number of yellow pigments do not differ significantly. As a result of the conducted studies, a correlation was revealed between the content of carotenoids and the content of chlorophyll a in most species. The data obtained indicate the presence of species specificity in the content of chlorophylls a, b and carotenoids. Also, the photosynthetic apparatus of woody plants is sensitive to light and water conditions.

Effects of chemical oxygen demand and chloramphenicol on attached microalgae growth: Physicochemical properties and microscopic mass transfer in biofilm

During the wastewater treatment and resource recovery process by attached microalgae, the chemical oxygen demand (COD) can cause biotic contamination in algal culture systems, which can be mitigated by adding an appropriate dosage of antibiotics. The transport of COD and additive antibiotic (chloramphenicol, CAP) in algal biofilms and their influence on algal physiology were studied. The results showed that COD (60 mg/L) affected key metabolic pathways, such as photosystem II and oxidative phosphorylation, improved biofilm autotrophic and heterotrophic metabolic intensities, increased nutrient demand, and promoted biomass accumulation by 55.9 %, which was the most suitable COD concentration for attached microalgae. CAP (5–10 mg/L) effectively stimulated photosynthetic pigment accumulation and nutrient utilization in pelagic microalgal cells. In conclusion, controlling the COD concentration (approximately 60 mg/L) in the medium and adding the appropriate CAP concentration (5–10 mg/L) are conducive to improving attached microalgal biomass production and resource recovery potential from wastewater.

Mycosporine-like Amino Acids in Palmaria palmata (Rhodophyta): Specific Implication of Usujirene in Photoprotection.

The effect of UV radiation on the accumulation of mycosporine-like amino acids (MAAs) and pigments was investigated on red macroalga Palmaria palmata cultivated for 21 days. The data were combined with the effect of NaNO3 to further investigate the synthesis of these nitrogenous compounds. A progressive decrease in both total MAA and pigment contents was observed, with a positive effect of nitrate supply. Usujirene was the only MAA exhibiting a significantly increasing content when exposed to UV radiation, changing from 9% to 24% of the total MAA's contribution, with no variation observed with NaNO3. This suggests a specific induction or synthesis pathway of usujirene for photoprotection, while the synthesis of other MAAs could have been limited by an insufficient amount of UV radiation and/or irradiance. The photoprotective ability of some MAAs could have been impacted by nitrogen starvation over time, resulting in a limited synthesis and/or potential use of MAAs as a nitrogen source for red macroalgae. The data confirmed the multiple effects of environmental factors on the synthesis of MAAs while providing new insights into the specific synthesis of usujirene, which could find an application in the cosmetics sector as natural sunscreen or an anti-ageing agent.

Alleviation of cotton growth suppression caused by salinity through biochar is strongly linked to the microbial metabolic potential in saline-alkali soil

Biochar is a typical soil organic amendment; however, there is limited understanding of its impact on the metabolic characteristics of microorganisms in saline-alkaline soil microenvironment, as well as the advantages and disadvantages of plant-microorganism interactions. To elucidate the mechanisms underlying the impact of saline-alkali stress on cotton, a 6-month pot experiment was conducted, involving the sowing of cotton seedlings in saline-alkali soil. Three different biochar application levels were established: 0 % (C0), 1 % (C1), and 2 % (C2). Results indicated that biochar addition improved the biomass of cotton plants, especially under C2 treatment; the dry weight of cotton bolls were 8.15 times that of C0. Biochar application led to a rise in the accumulation of photosynthetic pigments by 8.30–51.89 % and carbohydrates by 7.4–10.7 times, respectively. Moreover, peroxidase (POD) activity, the content of glutathione (GSH), and ascorbic acid (ASA) were elevated by 23.97 %, 118.39 %, and 48.30 % under C2 treatment, respectively. Biochar caused a reduction in Na+ uptake by 8.21–39.47 %, relative electrical conductivity (REC) of plants, and improved K+/Na+ and Ca2+/Na+ ratio indicating that biochar alleviated salinity-caused growth reduction. Additionally, the application of biochar enhanced the absorption intensity of polysaccharide fingerprints in cotton leaves and roots. Two-factor co-occurrence analysis indicated that the key differential metabolites connected to several metabolic pathways were L-phenylalanine, piperidine, L-tryptophan, and allysine. Interestingly, biochar altered the metabolic characteristics of saline-alkali soil, especially related to the biosynthesis and metabolism of amino acids and purine metabolism. In conclusion, this study demonstrates that biochar may be advantageous in saline soil microenvironment; it has a favorable impact on how plants and soil microbial metabolism interact.

Potential locus W and candidate gene McPRR2 associated with pericarp pigment accumulation in bitter gourd (Momordica charantia L.) revealed via BSA-seq analysis

Pericarp color is a prominent agronomic trait that exerts a significant impact on consumer and breeder preferences. Genetic analysis has revealed that the pericarp color of bitter gourd is a quantitative trait. However, the underlying mechanism for this trait in bitter gourd remains largely unknown. In the present study, we employed bulked segregant analysis (BSA) to identify the candidate genes responsible for bitter gourd pericarp color (specifically, dark green versus white) within F2 segregation populations resulting from the crossing of B07 (dark green pericarp) and A06 (white pericarp). Through genomic variation, genetic mapping, and expression analysis, we identified a candidate gene named McPRR2, which was a homolog of Arabidopsis pseudo response regulator 2 (APRR2) encoded by LOC111023472. Sequence alignment of the candidate gene between the two parental lines revealed a 15-bp nucleotide insertion in the coding region of LOC111023472, leading to a premature stop codon and potentially causing a loss-of-function mutation. qRT-PCR analysis demonstrated that the expression of McPRR2 was significantly higher in B07 compared to A06, and it was primarily expressed in the immature fruit pericarp. Moreover, overexpression of McPRR2 in tomato could enhance the green color of immature fruit pericarp by increasing the chlorophyll content. Consequently, McPRR2 emerged as a strong candidate gene regulating the bitter gourd pericarp color by influencing chlorophyll accumulation. Finally, we developed a molecular marker linked to pericarp color, enabling the identification of genotypes in breeding populations. These findings provided valuable insights into the genetic improvement of bitter gourd pericarp color.

Emerging roles and mechanisms of lncRNAs in fruit and vegetables.

With the development of genome sequencing technologies, many long non-coding RNAs (lncRNAs) have been identified in fruit and vegetables. lncRNAs are primarily transcribed and spliced by RNA polymerase II (PolII) or plant-specific PolIV/V, and exhibit limited evolutionary conservation. lncRNAs intricately regulate various aspects of fruit and vegetables, including pigment accumulation, reproductive tissue development, fruit ripening, and responses to biotic and abiotic stresses, through diverse mechanisms such as gene expression modulation, interaction with hormones and transcription factors, microRNA regulation, and involvement in alternative splicing. This review presents a comprehensive overview of lncRNA classification, basic characteristics, and, most importantly, recent advances in understanding their functions and regulatory mechanisms.

Effect of two biocontrols on the accumulation of phenolic compounds and leaf pigments in sorghum [<i>Sorghum bicolor</i> (L.) Moench] leaves in northern Côte d'Ivoire

The aim of this study was to find an alternative to chemical control by improving sorghum's natural defense system. The effect of two biocontrols, Vacciplant and Calliet, on phenolic compound and leaf pigment levels was evaluated. Vacciplant was prepared at concentrations of 0.675; 0.9; 1.125; 1.35 and 1.575 mg/mL, while Calliet was prepared at concentrations of 4 ; 8 ; 12 ; 16 and 20 mg/mL. After preparation, the biocontrol solutions were sprayed onto sorghum leaves 60 days old. Incubation times of 24, 48, 72 and 96 hours were observed after spraying. The sorghum leaves were then harvested and freeze-dried for quantification of total phenols and leaf pigments. Results showed that Vacciplant at a concentration of 1.125 mg/mL after 72 hours of incubation allowed to have the highest contents of total phenols (76.63 mg/g MF), while Calliet at concentration of 4.00 mg/mL after 24 hours of incubation allowed to have the highest contents of total phenols (67.28 mg/g MF). With regard to photosynthetic activity, the results also showed an increase in leaf pigment content following the application of biocontrols. The use of biocontrols could therefore be an interesting alternative to chemical control in sorghum.

Evaluation of larvicidal potential of Eritrean medicinal plants against Aedes aegypti.

Larvicidal activity of three Eritrean medicinal plants was evaluated against Aedes aegypti by conducting the bioassay using WHO methods. Efficacy of the plant extracts of O. hadiense, R. officinalis and C. spinarum was evaluated against 3rd instar Aedes aegypti larvae and mortality was recorded. LC50 and LC90 of the various plant extracts were also calculated using probit analysis. The morphological analysis of treated larvae was also performed. Extracts of O. hadiense, C. spinarium and R. officinalis were prepared using different solvents viz chloroform, 70% ethanol and water. Of the screened extracts, the chloroform extracts of O. hadiense exhibited the highest larvicidal activities and has the minimum LC50 and LC90 (24mg/ml and 198.411mg/ml respectively). Chloroform extract of C. spinarium exhibited the least larvicidal activity with maximum LC50 and LC90 (736.883mg/ml and 1188.699mg/ml respectively). Microscopic analysis confirmed the changes in the Aedes aegypti larvae caused by various plants extracts. An accumulation of dark pigmentation was observed in abdominal region and in the anal papillae after contact and also showed major structural damage such as destruction of the gut.

Integrated field application of T. viride, botanicals, and fungicides for managing early blight (Alternaria solani) and enhancement of plant growth, tuber nutritional quality, and potato yield

Early blight disease, incited by Alternaria solani, is the most deleterious foliar disease of potato and other solanaceous crops. Herein, to counter the early blight disease, T. viride (Tv), botanicals [neem (N) and garlic (G)], and fungicides [flusilazole (F) and kasugamycin (K)] in different combinations applied through tuber treatment (ST) and foliar spray (FS) were evaluated for disease suppression, germination percent, plant growth promotion, photosynthetic pigments, and yield components as well as the nutritional quality of tubers under field conditions during the 2018–2019 and 2019–2020 seasons. The results of two-year field experiments revealed that the combination of Tv+ F+ N (ST) + G+K (FS) efficiently suppressed the development of the early blight disease symptoms and recorded 21.63 and 18.37% disease severity (DS) as compared to untreated control, respectively at 30 days post-treatment (dpt). During the 2018–2019 seasons, potato tubers treated with Tv +F significantly increased tuber germination (97.78%), while Tv+ F+ N treated potato tubers exhibited 98.89% tuber germination during the 2019–2020 season. Additionally, the potato plants treated with the combination of Tv+ F+ N (ST) + G+K (FS) and challenge inoculated with A. solani positively increased plant growth promotion activities like shoot and root length, fresh and dry weight of shoot and root, number of branches/plant, and induced accumulation of photosynthetic pigments like chlorophyll-a, b, and total carotenoids content during both seasons. Furthermore, under A. solani inoculated conditions, treatment with Tv+ F+ N (ST) + G+K (FS) significantly increased all yield attributing traits and other parameters associated with tuber nutritional quality such as starch (1.79-and 1.68-fold, respectively), vitamin-C (1.51-and 1.58-fold, respectively), total nitrogen (1.31-and 1.33-fold, respectively), total phosphorus (1.53-and 1.60-fold, respectively), and total potassium content (1.42-and 1.45-fold, respectively) as compared to untreated control in both seasons. So, it can be concluded that among all the treatments, integrated treatment comprising of tuber treatment with T. viride, flusilazole, and neem extract + one foliar spray of garlic extract and kasugamycin was found very effective in reducing early blight disease, promoting the plant’s growth, and increasing the yield as well as nutritional quality of tubers in the field conditions. The inclusive results indicated that integrated application might be a sustainable and eco-friendly control strategy for potato growers to manage potato early blight and achieve higher yields and nutritional tubers.