The liquefaction potential of saturated sand can be significantly reduced by inducing partial saturation in the soil. Conventional soil liquefaction mitigation methods, namely soil densification, drainage, cementing, and groundwater lowering, pose environmental concerns and are challenging to apply to pre-existing structures. However, the microbially induced partial saturation (MIPS) method is emerging as a novel and eco-friendly approach to mitigate liquefaction. The MIPS method involves microbial denitrification, which produces nitrogen gas and results in a desaturating effect in the saturated soil. The current study conducted a series of stress-controlled undrained cyclic triaxial tests on saturated sandy soil and microbially-desaturated sandy soil under different relative densities and loading conditions. In addition, the study systematically analyzed the effects of temperature and pH on bacterial activity and the denitrification process. Batch experiments were conducted to establish a relationship between the initial nitrate concentration in the bacterial media and the resulting desaturation.Comprehensive analyses of cyclic resistance curves were performed to gain a thorough understanding. Additionally, the study conducts detailed analyses of the accumulation of excess pore pressure and the resulting axial strains and deformation patterns in both treated and untreated sand. This study demonstrates that the MIPS treatment considerably enhances the liquefaction resistance of treated sand.