Chlorophyll Accumulation Research Articles

IReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation.

Seedling de-etiolation is one of the key stages of the plant life cycle, characterized by a strong rearrangement of the plant development and metabolism. The conversion of dark accumulated protochlorophyllide to chlorophyll in etioplasts of de-etiolating plants is taking place in order of ns to µs after seedlings illumination, leading to detectable increase of chlorophyll levels in order of minutes after de-etiolation initiation. The highly complex chlorophyll biosynthesis integrates number of regulatory events including light and hormonal signaling, thus making de-etiolation an ideal model to study the underlying molecular mechanisms. Here we introduce the iReenCAM, a novel tool designed for non-invasive fluorescence-based quantitation of early stages of chlorophyll biosynthesis during de-etiolation with high spatial and temporal resolution. iReenCAM comprises customized HW configuration and optimized SW packages, allowing synchronized automated measurement and analysis of the acquired fluorescence image data. Using the system and carefully optimized protocol, we show tight correlation between the iReenCAM monitored fluorescence and HPLC measured chlorophyll accumulation during first 4h of seedling de-etiolation in wild type Arabidopsis and mutants with disturbed chlorophyll biosynthesis. Using the approach, we demonstrate negative effect of exogenously applied cytokinins and ethylene on chlorophyll biosynthesis during early de-etiolation. Accordingly, we identify type-B response regulators, the cytokinin-responsive transcriptional activators ARR1 and ARR12 as negative regulators of early chlorophyll biosynthesis, while contrasting response was observed in case of EIN2 and EIN3, the components of canonical ethylene signaling cascade. Knowing that, we propose the use of iReenCAM as a new phenotyping tool, suitable for quantitative and robust characterization of the highly dynamic response of seedling de-etiolation.

CsRVE1 promotes seasonal greening of albino Camellia sinensis cv. Huangkui by activating chlorophyll biosynthesis.

Seasonal greening is a crucial survival strategy for albino tea cultivars, during which dysfunctional chloroplasts recover and chlorophyll biosynthesis increases in albino leaves. However, the regulatory mechanisms of seasonal greening in albino tea plants remain unclear. Here, we report that CsRVE1, a nuclear-located Myb-like transcription factor, can positively modulate the seasonal greening of albino Camellia sinensis cv. Huangkui leaves by activating the expression of genes involved in light harvesting and chlorophyll biosynthesis. The transcriptional expression of CsRVE1 increased during seasonal greening and was tightly correlated with increases in the expression of genes involved in light harvesting (CsLhcb) and chlorophyll biosynthesis (CsCHLH, CsHEMA1 and CsCAO). In vivo and in vitro molecular analyses showed that CsRVE1 can directly bind to the promoters of CsLhcb, CsCHLH and CsPORA, eventually leading to chlorophyll accumulation in tea leaves. Furthermore, transient suppression of CsRVE1 in tea leaves led to a decrease in target gene expression. In contrast, the overexpression of CsRVE1 in Arabidopsis led to chlorophyll increases and the activation of AtLhcb, AtPORA, AtCHLH, etc. These results identify CsRVE1 as an important promoter of seasonal greening that functions by regulating genes involved in chlorophyll biosynthesis in albino tea plants and shed new light on the regulatory mechanisms of leaf phenotypes in plants.

Auxin inhibits chlorophyll accumulation through ARF7-IAA14-mediated repression of chlorophyll biosynthesis genes in Arabidopsis.

Auxin is a well-known important phytohormone in plant that plays vital roles in almost every development process throughout plant lifecycle. However, the effect of auxin on the metabolism of chlorophyll, one of the most important pigments involved in the photosynthesis, was intertwined and the underlying mechanism remained to be explored. Here, we found the auxin-defective yuc2 yuc6 double mutant displayed dark-green leaf color with higher chlorophyll content than wildtype, suggesting a negative regulatory role of auxin in chlorophyll biosynthesis. The chloroplast number and structure in mesophyll cells were altered and the photosynthetic efficiency was improved in yuc2 yuc6. In addition, the chlorophyll level was significantly improved during seedling de-etiolation in yuc2 yuc6 mutant, and decreased dramatically under IAA treatment, confirming the inhibitory role of auxin in chlorophyll biosynthesis. The analyses of gene expression in mature leaves and de-etiolation seedlings suggested that auxin suppressed the expression of many chlorophyll biosynthesis genes, especially PROTOCHLOROPHYLLIDE OXIDOREDUCTASE A (PORA) and GENOMES UNCOUPLED 5 (GUN5). Yeast-one-hybrid and luciferase assays demonstrated that the AUXIN RESPONSE FACTOR 2 (ARF2) and ARF7 bind to the promoter of PORA and GUN5 to suppress their expression with the help of INDOLE-3-ACETIC ACID14 (IAA14). Collectively, our research explicitly unraveled the direct inhibitory role of auxin in chlorophyll biosynthesis, and provided new insight into the interplay between auxin signaling and chlorophyll metabolism.

The influence of increased seawater HCO3− concentration on the survival, growth and physiology of the eelgrass Zostera marina

Inorganic resources are a key determining factor in the growth process of seagrass; however, the process and mechanisms involved in the promoting effect of increased seawater HCO3− concentration on seagrass growth and propagation under controlled conditions are poorly understood. We subjected shoots of the eelgrass Zostera marina to different seawater HCO3− levels [2 (control), 5, 10, 15 and 20 mmol L−1] for 6 weeks under controlled, mesocosm conditions. We measured eelgrass response in terms of survivorship, growth, propagation, productivity, carbonic anhydrase, leaf pigmentation and carbohydrate concentrations. The ramet frequency of Z. marina reached 58.7% when exposed to 5 mmol L−1, which was 1.3 times higher than that of shoots under the control condition. Survival analysis combined with growth assessment suggested that the optimal range of seawater HCO3− concentration for the propagation of Z. marina shoots was 4.8–5.5 mmol L−1. The promotion of survival and propagation of Z. marina that stemmed from seawater HCO3− concentration mainly depended on the increase in chlorophyll content and accumulation of nonstructural carbohydrate. The total chlorophyll content of Z. marina leaves exposed to 5 mmol L−1 was 1.1 times higher than that of shoots under the control condition. The results provide valuable data that could prove helpful in the development of efficient artificial propagation technology for Z. marina shoots.

Effect of ultra-violet light radiation on Scenedesmus vacuolatus growth kinetics, metabolic performance, and preliminary biodegradation study.

This study presents the effect of ultra-violet (UV) light radiation on the process kinetics, metabolic performance, and biodegradation capability of Scenedesmus vacuolatus. The impact of the UV radiation on S. vacuolatus morphology, chlorophyll, carotenoid, carbohydrates, proteins, lipid accumulation, growth rate, substrate affinity and substrate versatility were evaluated. Thereafter, a preliminary biodegradative potential of UV-exposed S. vacuolatus on spent coolant waste (SCW) was carried out based on dehydrogenase activity (DHA) and total petroleum hydrocarbon degradation (TPH). Pronounced structural changes were observed in S. vacuolatus exposed to UV radiation for 24h compared to the 2, 4, 6, 12 and 48h UV exposure. Exposure of S. vacuolatus to UV radiation improved cellular chlorophyll (chla = 1.89-fold, chlb = 2.02-fold), carotenoid (1.24-fold), carbohydrates (4.62-fold), proteins (1.44-fold) and lipid accumulations (1.40-fold). In addition, the 24h UV exposed S. vacuolatus showed a significant increase in substrate affinity (1/Ks) (0.959), specific growth rate (µ) (0.024h-1) and biomass accumulation (0.513g/L) by 1.50, 2 and 1.9-fold respectively. Moreover, enhanced DHA (55%) and TPH (100%) degradation efficiency were observed in UV-exposed S. vacuolatus. These findings provided major insights into the use of UV radiation to enhance S. vacuolatus biodegradative performance towards sustainable green environment negating the use of expensive chemicals and other unfriendly environmental practices.

The effect of fungicides on the physiological and biochemical state of lupine plants after seed treatment

Goal. To assess the effect of fungicides on the content of chlorophyll and the activity of antioxidant enzymes (peroxidase, catalase) in lupine plants after seed treatment.
 Methods. Laboratory and vegetation studies were carried out in the laboratory of analytical chemistry of pesticides of pesticides of the Institute of Plant Protection. The seeds of yellow lupine (Lupinus luteus L., variety Obriy) were treated with combined fungicides from different chemical classes: triazoles (cyproconazole, difenoconazole, tebuconazole, prothioconazole), phenylpyrroles (fludioxonil), carboxamides (carboxin), dithiocarbamates (thiram). Determination of the content of fungicides in plants was carried out using chromatographic methods of analysis. Chlorophyll content, peroxidase and catalase activity were determined by physicochemical methods according to generally accepted methods.
 Results. Fungicides activated the antioxidant systems of plants, depending on their physicochemical properties (in particular, the octanol-water partition coefficient Kow) and their content in plants (C, mg/kg). An increase in peroxidase activity (up to 89% compared to the control) was established within 30 days, which indicates the activation of antioxidant processes aimed at maintaining oxidative homeostasis in plants. The change in catalase activity under the influence of fungicides was inversely correlated with peroxidase activity, which is explained by the formation of adaptive mechanisms of ROS homeostasis. The high activity of peroxidase, compared to catalase, testifies to the important role of this enzyme in the redox reactions of plant resistance under the influence of fungicides. The total chlorophyll content under the action of fungicides, increased by 6—20%, compared to the control.
 Conclusions. Seed treatment with fungicides affects the course of redox reactions in plants, causing changes in the activity of key enzymes. The high activity of peroxidase against the background of fluctuations in catalase activity indicates the direct effect of fungicides on the state of antioxidant systems and the important role of peroxidase in maintaining ROS homeostasis. The stimulatory effect of fungicides on the accumulation of chlorophyll at the initial stages of growth of lupine plants was established, the content of which exceeded the control indicator by 6—20% by development phase. Changes in the activity of antioxidant enzymes (catalase and peroxidase) and the content of chlorophyll can serve as criteria for determining the levels of adaptation of lupine plants to the stress effect of fungicides.

Dynamics of accumulation of photosynthetic pigments in leaves <i>Trititrigia Cziczinii</i> Tzvel.

The productivity of plants depends on the efficiency of photosynthetic pigments, of which chlorophyll is the most important. In the conditions of sod-podzolic medium loamy soils of the Moscow region, the features of the accumulation of photosynthetic pigments (chlorophyll a and chlorophyll b) and the photosynthetic apparatus of a new synthetic grain crop Trititrigia cziczinii Tzvel. were studied. (Pamyati Lyubimovoj variety, line № 3202) compared to the winter wheat variety (Rubezhnaya) obtained by remote hybridization. The dynamics of the accumulation of chlorophyll a and chlorophyll b in the leaves of grain crops was measured during the generative development of plants starting from the earing phase (BBCH-scale code 52-53) on a spectrophotometer. The research results showed that all the studied varieties have their peaks of accumulation of the amount of chlorophyll a and chlorophyll b, depending on their biological characteristics. In winter wheat, the maximum amount of the sum of the two pigments fell on July 2 and amounted to 3,3 mg/g of raw leaf mass. In the trititrigia variety of the Pamyati lyubimovoj, the peak of pigment accumulation was noted almost a month later (July 30). The amount of pigments was 4,45 mg/g of raw mass. Trititrigia line № 3202 accumulated the maximum amount of chlorophyll (4,20 mg/g of raw leaf mass) even later than the Pamyati Lyubimovoj variety – August 5. The higher content of photosynthetic pigments in the cells of trititrigia leaves, compared with winter wheat, may indicate its high adaptive characteristics. And the peaks of chlorophyll accumulation, shifted by almost a month, indicate the long-term work of the assimilation apparatus and the uniqueness of the new synthetic culture. The leaf area measured in a straight linear way was different for all the studied objects. The largest leaf area was noted in the variety of trititrigia in Pamyati Lyubimovoj – 21,2 thousand m2/ha on July 13. The maximum leaf area at line № 3202 was 14.4 thousand m2/ha, then it gradually decreased. Rubezhnaya winter wheat had a significantly lower leaf area compared to trititrigia samples (the maximum was 6.1 thousand m2/ha), then the activity of the active assimilating surface decreased.

Comparative Transcriptomic and Physiological Analyses Reveal Key Factors for Interstocks to Improve Grafted Seedling Growth in Tangor.

Interstock is an important agronomic technique for regulating plant growth and fruit quality, and overcoming the incompatibility between rootstocks and scions; however, the underlying mechanisms remain largely unknown. In this study, the effects and regulatory mechanisms of tangor grafting, with and without interstocks, on the growth and development of scions were analyzed by combining morphology, physiology, anatomy and transcriptomics. Morphological and physiological analyses showed that interstocks ('Aiyuan 38' and 'Daya') significantly improved the growth of seedlings, effectively enhanced the foliar accumulation of chlorophyll and carotenoids, and increased the thickness of leaf tissues. Using 'Aiyuan 38' as the interstock, photosynthetic efficiency and starch content of citrus seedlings improved. Transcriptomics showed that genes related to photosynthesis and photosynthetic antenna proteins were upregulated in interstock-treated seedlings, with significant upregulation of photosystem PSI- and PSII-related genes. In addition, multiple key genes may be involved in plant hormone signaling, starch and sucrose metabolism, and transcriptional regulation. Taken together, these findings provide novel insights into the role of interstocks in regulating and contributing to the growth and development of grafted seedlings, and will further define and deploy candidate genes to explore the mechanisms of rootstock-interstock-scion interactions.

Effects of Cd treatment on morphology, chlorophyll content and antioxidant enzyme activity of Elymus nutans Griseb., a native plant in Qinghai-Tibet Plateau

ABSTRACT Cd pollution is a global environmental problem. However, the response mechanism of the alpine plant Pelagia under Cd stress remains unclear. Therefore, in this study, a native plant(Elymus nutans Griseb.) of the Qinghai-Tibet Plateau was used as the material to quantify plant height, leaf number, length of leaf, crown width, root number, biomass, Dry weight malondialdehyde (MDA), free proline, superoxide dismutase (SOD), ascorbate enzyme (APX), catalase (CAT) and chlorophyll contents under different Cd concentrations. The results showed that the growth of Elymus nutans Griseb. was a phenomenon of “low concentration promotes growth, high concentration inhibited growth” under Cd treatment. It meant that 10 mg·L−1 Cd promoted the growth of leaf number, plant height, crown width and tiller number, while 40 mg·L−1 Cd inhibited the growth of root number and biomass of Elymus nutans Griseb. compare with the control. The MDA content, free proline content, SOD activity, APX activity and CAT activity of Elymus nutans Griseb. was increased with the increase of Cd treatment concentration to resist the oxidative damage caused by Cd to the body. At the same time, the accumulation of chlorophyll A, chlorophyll B and chlorophyll AB was decreased with the increase of Cd stress concentration. In addition, the carotenoid content did not change much between the control group and the treatment group, indicating that Cd treatment had little effect on it. The results could provide a reference for the mechanism of heavy metal resistance and the selection and improvement of Cd -resistant varieties of Elymus nutans Griseb.

Exogenous abscisic acid and sodium nitroprusside regulate flavonoid biosynthesis and photosynthesis of Nitraria tangutorum Bobr in alkali stress.

Abscisic acid (ABA) and nitric oxide (NO) are involved in mediating abiotic stress-induced plant physiological responses. Nitraria tangutorum Bobr is a typical salinized desert plant growing in an arid environment. In this study, we investigated the effects of ABA and NO on N.tangutorum seedlings under alkaline stress. Alkali stress treatment caused cell membrane damage, increased electrolyte leakage, and induced higher production of reactive oxygen species (ROS), which caused growth inhibition and oxidative stress in N.tangutorum seedlings. Exogenous application of ABA (15μm) and Sodium nitroprusside (50μm) significantly increased the plant height, fresh weight, relative water content, and degree of succulency in N.tangutorum seedlings under alkali stress. Meanwhile, the contents of ABA and NO in plant leaves were significantly increased. ABA and SNP can promote stomatal closure, decrease the water loss rate, increase leaf surface temperature and the contents of osmotic regulator proline, soluble protein, and betaine under alkali stress. Meanwhile, SNP more significantly promoted the accumulation of chlorophyll a/b and carotenoids, increased quantum yield of photosystem II (φPSII) and electron transport rate (ETRII) than ABA, and decreased photochemical quenching (qP), which improved photosynthetic efficiency and accelerated the accumulation of soluble sugar, glucose, fructose, sucrose, starch, and total sugar. However, compared with exogenous application of SNP in the alkaline stress, ABA significantly promoted the transcription of NtFLS/NtF3H/NtF3H/NtANR genes and the accumulation of naringin, quercetin, isorhamnetin, kaempferol, and catechin in the synthesis pathway of flavonoid metabolites, and isorhamnetin content was the highest. These results indicate that both ABA and SNP can reduce the growth inhibition and physiological damage caused by alkali stress. Among them, SNP has a better effect on the improvement of photosynthetic efficiency and the regulation of carbohydrate accumulation than ABA, while ABA has a more significant effect on the regulation of flavonoid and anthocyanin secondary metabolite accumulation. Exogenous application of ABA and SNP also improved the antioxidant capacity and the ability to maintain Na+/K+ balance of N. tangutorum seedlings under alkali stress. These results demonstrate the beneficial effects of ABA and NO as stress hormones and signaling molecules that positively regulate the defensive response of N. tangutorum to alkaline stress.

Significant increases in Donghong kiwifruit yield by a novel umbrella-shaped trellis system and identification of associated molecular mechanisms.

China is the largest kiwifruit producer in the world, accounting for more than half of the total. However, in terms of yield per unit area, China is much lower than the global average and lags behind that of other countries. Yield improvement is of critical importance for the current kiwifruit industry in China. In this study, an improved overhead pergolas trellis (OPT) system, namely, the umbrella-shaped trellis (UST) system, was developed for Donghong kiwifruit, which is now the second most popular and widely cultivated red-fleshed kiwifruit in China. Surprisingly, the estimated yield on the UST system was more than two times higher than that with a traditional OPT, while the external fruit quality was maintained and the internal fruit quality was improved. One of the mechanisms contributing to the yield improvement was the significant promotion of the vegetative growth of canes at 6 ~ 10mm in diameter by the UST system. The upper canopy of the UST treatment served as a natural shading condition for the lower fruiting canopy and thus had positive effects on the accumulation of chlorophylls and total carotenoids in the fruiting canopy. The most productive zones on the fruiting canes (6 ~ 10mm in diameter) contained significantly higher (P < 0.05) levels of zeatin riboside (ZR) and auxin (IAA) and ratios of ZR/gibberellin (GA), ZR/abscisic acid (ABA), and ABA/GA. A relatively high carbon/nitrogen ratio may promote the flower bud differentiation process of Donghong kiwifruit. The outcomes of this study provide a scientific basis for manifold increase in production of kiwifruit and contribute to the sustainability of the kiwifruit industry.

Dynamic changes of the contents of photoprotective substances and photosynthetic maturation during leaf development of evergreen tree species in subtropical forests.

Many studies have investigated the photoprotective and photosynthetic capacity of plant leaves, but few have simultaneously evaluated the dynamic changes of photoprotective capacity and photosynthetic maturation of leaves at different developmental stages. As a result, the process between the decline of photoprotective substances and the onset of photosynthetic maturation during plant leaf development are still poorly understood, and the relationship between them has not been quantitatively described. In this study, the contents of photoprotective substances, photosynthetic pigment content and photosynthetic capacity of leaves at different developmental stages from young leaves to mature leaves were determined by spatio-temporal replacement in eight dominant tree species in subtropical evergreen broadleaved forests. The correlation analysis found that the data sets of anthocyanins, flavonoids, total phenolics and total antioxidant capacity were mainly distributed on one side of the symmetry axis (y=x), while the data sets of flavonoids, total phenolics and total antioxidant capacity were mainly distributed on both sides of the symmetry axis (y=x). In addition, the content of photoprotective substances in plant leaves was significantly negatively correlated with photosynthetic pigment content and photosynthetic capacity but was significantly positively correlated with dark respiration rate (Rd). When chlorophyll accumulated to ~50% of the final value, the photoprotective substance content and Rd of plant leaves reached the lowest level, and anthocyanins disappeared completely; in contrast, the photosynthetic capacity reached the highest level. Our results suggest that anthocyanins mainly play a light-shielding role in the young leaves of most plants in subtropical forests. In addition, 50% chlorophyll accumulation in most plant leaves was the basis for judging leaf photosynthetic maturity. We also believe that 50% chlorophyll accumulation is a critical period in the transition of plant leaves from high photoprotective capacity (high metabolic capacity, low photosynthetic capacity) to low photoprotective capacity (low metabolic capacity, high photosynthetic capacity).

Nitrogen application improves salt tolerance of grape seedlings via regulating hormone metabolism.

Salt stress is a dominant environmental factor that restricts the growth and yield of crops. Nitrogen is an essential mineral element for plants, regulates various physiological and biochemical processes, and has been reported to enhance salt tolerance in plants. However, the crosstalk between salt and nitrogen in grapes is not well understood. In this study, we found that nitrogen supplementation (0.01 and 0.1 mol L-1 NH4 NO3 ) significantly increased the accumulation of proline, chlorophyll, Na+ , NH4 + , and NO3 - , while it reduced the malondialdehyde content and inhibited photosynthetic performance under salt stress conditions (200 mmol L-1 NaCl). Further transcriptome and metabolome analyses showed that a total of 4890 differentially expressed genes (DEGs) and 753 differently accumulated metabolites (DAMs) were identified. Joint omics results revealed that plant hormone signal transduction pathway connected the DEGs and DAMs. In-depth analysis revealed that nitrogen supplementation increased the levels of endogenous abscisic acid, salicylic acid, and jasmonic acid by inducing the expression of 11, 4, and 13 genes related to their respective biosynthesis pathway. In contrast, endogenous indoleacetic acid content was significantly reduced due to the remarkable regulation of seven genes of its biosynthetic pathway. The modulation in hormone contents subsequently activated the differential expression of 13, 10, 12, and 29 genes of the respective downstream hormone signaling transduction pathways. Overall, all results indicate that moderate nitrogen supplementation could improve salt tolerance by regulating grape physiology and endogenous hormone homeostasis, as well as the expression of key genes in signaling pathways, which provides new insights into the interactions between mineral elements and salt stress.

SmCIP7, a COP1 interactive protein, positively regulates anthocyanin accumulation and fruit size in eggplant

In higher plants, COP1 (Constitutively Photomorphogenic 1) acts as a central regulator of light-signaling networks and globally conditions the target proteins via the ubiquitin-proteasome pathway. However, the function of COP1-interacting proteins in light-regulated fruit coloration and development remains unknown in Solanaceous plants. Here, a COP1-interacting protein-encoding gene, SmCIP7, expressed specifically in the eggplant (Solanum melongena L.) fruit, was isolated. Gene-specific silencing of SmCIP7 using RNA interference (RNAi) significantly altered fruit coloration, fruit size, flesh browning, and seed yield. SmCIP7-RNAi fruits showed evident repression of the accumulation of anthocyanins and chlorophyll, indicating functional similarities between SmCIP7 and AtCIP7. However, the reduced fruit size and seed yield indicated SmCIP7 had evolved a distinctly new function. With the comprehensive application of HPLC-MS, RNA-seq, qRT-PCR, Y2H, BiFC, LCI, and dual-luciferase reporter system (DLR™), it was found that SmCIP7, a COP1 interactive protein in light signaling promoted anthocyanin accumulation, probably by regulating the transcription of SmTT8. Additionally, the drastic up-regulation of SmYABBY1, a homologous gene of SlFAS, might account for the strongly retarded fruit growth in SmCIP7-RNAi eggplant. Altogether, this study proved that SmCIP7 is an essential regulatory gene to modulate fruit coloration and development, serving as a key gene locus in eggplant molecular breeding.

Nocardiopsis lucentensis and thiourea co-application mitigates arsenic stress through enhanced antioxidant metabolism and lignin accumulation in rice

Arsenic (As) is a group-1 carcinogenic metalloid that threatens global food safety and security, primarily via its phytotoxicity in the staple crop rice. In the present study, ThioAC, the co-application of thiourea (TU, a non-physiological redox regulator) and N. lucentensis (Act, an As-detoxifying actinobacteria), was evaluated as a low-cost approach for alleviating As(III) toxicity in rice. To this end, we phenotyped rice seedlings subjected to 400 mg kg−1 As(III) with/without TU, Act or ThioAC and analyzed their redox status. Under As-stress conditions, ThioAC treatment stabilized photosynthetic performance, as indicated by 78 % higher total chlorophyll accumulation and 81 % higher leaf biomass, compared with those of As-stressed plants. Further, ThioAC improved root lignin levels (2.08-fold) by activating the key enzymes of lignin biosynthesis under As-stress. The extent of reduction in total As under ThioAC (36 %) was significantly higher than TU (26 %) and Act (12 %), compared to those of As-alone treatment, indicating their synergistic interaction. The supplementation of TU and Act activated enzymatic and non-enzymatic antioxidant systems, respectively, with a preference for young (TU) and old (Act) leaves. Additionally, ThioAC activated enzymatic antioxidants, specifically GR (∼3-fold), in a leaf-age specific manner and suppressed ROS-producing enzymes to near-control levels. This coincided with 2-fold higher induction of polyphenols and metallothionins in ThioAC-supplemented plants, resulting in improved antioxidant defence against As-stress. Thus, our findings highlighted ThioAC application as a robust, cost-effective ameliorative strategy, for achieving As-stress mitigation in a sustainable manner.

Genomic Survey and Expression Analysis of GLKs in Watermelon (Citrullus lanatus)

Golden2-like (GLK) genes positively regulate chloroplast development, increase crop yields, and improve fruit quality. However, there has been no comprehensive identification and characterization of GLKs in watermelon. In this study, a total of 48 ClGLKs were identified in the watermelon genome. Based on phylogenetic analysis, they were divided into five groups. ClGLKs within the same group showed a similar gene structure and conserved motif compositions. Promoter analysis indicated that cis-elements responsive to light were the most abundant, though cis-elements associated with hormones, stress, and developmental regulation were also identified in ClGLKs promoters. Expression analysis indicated significant responses of some ClGLKs to drought and CGMMV stress, suggesting that these genes may participate in responses to biotic and abiotic stresses. Phenotypic analyses revealed enhanced chloroplast development and increased thylakoid density and chlorophyll content in the pericarp of a “dark green” watermelon cultivar. ClGLK8 was identified as the homolog of GLK1-2, the genes that promote chloroplast development and chlorophyll biosynthesis in fruits, and showed significantly increased expression in accordance with chloroplast development and chlorophyll accumulation. Our results provide detailed knowledge of the ClGLKs, which will enhance efforts to further improve the fruit quality of watermelon.

Hormesis effects in tomato plant growth and photosynthesis due to acephate exposure based on physiology and transcriptomic analysis.

Hormesis is a common phenomenon in toxicology described as low-dose stimulation due to a toxin which causes inhibition at a high dose. Pesticide hormesis in plants has attracted considerable research interest in recent years; however, the specific mechanism has not yet been clarified. Acephate is an organophosphorus insecticide that is used worldwide. Here, hormesis in tomato (Solanum lycopersicum L.) plant growth and photosynthesis after acephate exposure is confirmed, as stimulation occurred at low stress levels, whereas inhibition occurred after exposure to high concentrations. We found that low acephate concentration (5-fold lower than recommended application dosage) could enhance chlorophyll biosynthesis and stimulate photosynthesis effects, and thus improve S. lycopersicum growth. A high level of acephate (5-fold higher than recommended application dosage) stress inhibited chlorophyll accumulation, decreased photosystem II efficiency and blocked antioxidant reactions in leaves, increasing reactive oxygen species levels and damaging plant growth. Transcriptomic analysis and quantitative real-time PCR results revealed that the photosynthesis - antenna proteins pathway played a crucial role in the hormesis effect, and that LHCB7 as well as LHCP from the pathway were the most sensitive to acephate hormesis. Our results showed that acephate could induce hormesis in tomato plant growth and photosynthesis, and that photosystem II and the photosynthesis - antenna proteins pathway played important roles in hormesis. These results provide novel insights into the scientific and safe application of chemical pesticides, and new guidance for investigation into utilizing pesticide hormesis in agriculture. © 2023 Society of Chemical Industry.

Simultaneous carbon dioxide sequestration and nitrate removal by Chlorella vulgaris and Pseudomonas sp. consortium

Carbon dioxide and nitrogen oxides are the main components of fossil flue gas causing the most serious environmental problems. Developing a sustainable and green method to treat carbon dioxide and nitrogen oxides of flue gas is still challenging. Here, a co-cultured microalgae/bacteria system, Chlorella vulgaris and Pseudomonas sp., was developed for simultaneous sequestration of CO2 and removal of nitrogen oxides from flue gas, as well as producing valuable microalgae biomass. The co-cultured Chlorella vulgaris and Pseudomonas sp. showed the highest CO2 fixation and NO3−-N removal rate of 0.482 g L−1d−1 and 129.6 mg L−1d−1, the total chlorophyll accumulation rate of 65.6 mg L−1 at the initial volume ratio of Chlorella vulgaris and Pseudomonas sp. as 1:10. The NO3−-N removal rate can be increased to 183.5 mg L−1d−1 by continuous addition of 0.6 g L−1d−1 of glucose, which was 37% higher than that of co-culture system without the addition of glucose. Photosynthetic activity and carbonic anhydrase activity of Chlorella vulgaris were significantly increased when co-cultured with Pseudomonas sp. Excitation–emission matrix (EEM) fluorescence spectroscopy indicated that the humic acid-like substances released from Pseudomonas sp. could increase the growth of microalgae. This work provides an attractive way to simultaneously treatment of CO2 and NOX from flue gas to produce valuable microalgal biomass.

High Light Intensity Triggered Abscisic Acid Biosynthesis Mediates Anthocyanin Accumulation in Young Leaves of Tea Plant (Camellia sinensis)

There is increasing interest in the production and consumption of tea (Camellia sinensis L.) processed from purple-leaved cultivar due to their high anthocyanin content and health benefits. However, how and why seasonal changes affect anthocyanin accumulation in young tea leaves still remains obscured. In this study, anthocyanin and abscisic acid (ABA) contents in young leaves of Zifuxing 1 (ZFX1), a cultivar with new shoots turning to purple in Wuyi Mountain, a key tea production region in China, were monitored over four seasons. Young leaves produced in September were highly purplish, which was accompanied with higher anthocyanin and ABA contents. Among the environmental factors, the light intensity in particular was closely correlated with anthocyanin and ABA contents. A shade experiment also indicated that anthocyanin content significantly decreased after 168 h growth under 75% shade, but ABA treatment under the shade conditions sustained anthocyanin content. To confirm the involvement of ABA in the modulation of anthocyanin accumulation, anthocyanin, carotenoids, chlorophyll, ABA, jasmonic acid (JA), and salicylic acid (SA) in the young leaves of four cultivars, including ZFX1, Zijuan (ZJ), wherein leaves are completely purple, Rougui (RG) and Fudingdabaicha (FDDB) wherein leaves are green, were analyzed, and antioxidant activities of the leaf extracts were tested. Results showed that ABA, not other tested hormones, was significantly correlated with anthocyanin accumulation in the purple-leaved cultivars. Cultivars with higher anthocyanin contents exhibited higher antioxidant activities. Subsequently, ZFX1 plants were grown under full sun and treated with ABA and fluridone (Flu), an ABA inhibitor. ABA treatment elevated anthocyanin level but decreased chlorophyll contents. The reverse was true to those treated with Flu. To pursue a better understanding of ABA involvement in anthocyanin accumulation, RNA-Seq was used to analyze transcript differences among ABA- or Flu-treated and untreated ZFX1 plants. Results indicated that the differentially expressed genes in ABA or Flu treatment were mainly ABA signal sensing and metabolism-related genes, anthocyanin accumulation-related genes, light-responsive genes, and key regulatory MYB transcription factors. Taking all the results into account, a model for anthocyanin accumulation in ZFX1 cultivar was proposed: high light intensity caused reactive oxygen stress, which triggered the biosynthesis of ABA; ABA interactions with transcription factors, such as MYB-enhanced anthocyanin biosynthesis limited chlorophyll and carotenoid accumulation; and transport of anthocyanin to vacuoles resulting in the young leaves of ZFX1 with purplish coloration. Further research is warranted to test this model.

Overexpression of PagGRF11 in poplar resulted in excessive accumulation of chlorophyll and inhibited seedling de-etiolation

Overexpression of PagGRF11 in poplar resulted in excessive accumulation of chlorophyll and inhibited seedling de-etiolation