Accumulation Of Amounts Research Articles

Analysis of Waist-to-Hip Ratio with Total Cholesterol Levels in Obese and Non-Obese Employees at El Tari Airport Kupang

Increased cholesterol levels are one of the causes of obesity. One type of obesity is central obesity, characterized by the amount of accumulation of fat in the abdominal area. Waist-to-Hip Ratio (WHR) measurement is more potent than Body Mass Index (BMI) in predicting cardiovascular disease risk. WHR is also used to identity the occurrence of central obesity, which is a risk factor for elevated cholesterol levels. This study aimed to analyze the difference in total cholesterol levels in the group of normal and above normal WHR employees at El Tari Airport, Kupang. This study was an observational analytic type with the cross-sectional method and used venous blood samples to measure total cholesterol levels and WHR. Data analysis using independent sample t-test. The results of the independent sample t-test showed a significant difference in total cholesterol levels between obese employees (above normal WHR) and non-obese employees (normal WHR). The significance result is 0.027 (p < 0.05) in the independent sample t-test. This indicates that WHR can be used to identify central obesity in a person and also person with above normal WHR tend to have high total cholesterol levels.

Numerical simulation and field study on predicting wind-blown sand accumulation in sand mitigation measures of the Ganquan railway

The accumulation of sand induced by wind poses a significant challenge to the safety and maintenance of railways in arid and desert regions. Accurate calculation and prediction of sand accumulation are crucial for ensuring continuous railway operation. This research is centered on the region significantly impacted by sand accumulation along the Ganquan Railway. Wind speed, wind direction, and sand carrying capacity data near this section were monitored. Using the collected wind speed, wind direction, and wind-sand flow density data, numerical simulations were conducted using the Computational Fluid Dynamics (CFD) method to predict the amount of sand accumulation within the sand mitigation measures of the Ganquan Railway. Monitoring results indicate that the dominant wind direction in spring and summer is due west, while in autumn and winter it is southwest, with an average wind speed of 12 m/s. A positive correlation was observed between wind-sand flow density and wind speed. The wind-sand flow density above 2 m was nearly zero, indicating that the wind-sand flow structure is concentrated within 2 m from the ground, with an average wind-sand flow density of 3.50×10−5 kg/m3. Through numerical simulation, the characteristics of the wind field and sand accumulation distribution within the calculation domain were determined. A relationship equation between sand accumulation mass and width over time was derived. Initially, the sand accumulation width increases uniformly and then stabilizes, while the sand accumulation mass rises uniformly to a plateau before in-creasing rapidly. From these findings, the optimal period for sand removal was identified as between 350 and 450 days after the sand mitigation measures are put into operation.

All-natural, thermally conductive, flame-retardant electrical insulating lignocellulose-mica composite film fabricated via in-situ mineralization

Miniaturized, integrated, and functional electronic devices generate a large amount of heat accumulation during operation, which significantly affects the reliability, durability, and safety of electronic devices. In severe cases, it may even lead to fires. Therefore, the development of insulation composite materials with thermal conductivity and flame-retardant properties is particularly important. In this study, we propose an in-situ mineralization strategy to prepare insulation composite materials with thermal conductivity and flame-retardant properties. By dissolving and preparing a gel film, Al3+ and Zn2+ retained inside the lignocellulose are in situ mineralized to form Al(OH)3 and ZnO, and introducing inorganic blocks with high insulation performance (industrial waste mica tailings and synthetic mica). The prepared film has excellent tensile strength (117.4 MPa), low dielectric loss, high dielectric strength (83.8 kV mm−1), high insulation resistance, four times higher thermal conductivity than the unmineralized film, and excellent flame-retardant properties. Meanwhile, the environmental impact of the composite film is much lower than that of petrochemical-based plastic films and can easily degrade under natural conditions within 32 days. It is a composite material that meets the standards of the new electrical insulation field, and the in-situ mineralization strategy can also provide ideas for the preparation of high-performance lignocellulose-based materials.

The relationship of visceral adiposity with endothelial functions and subclinical atherosclerosis in obese individuals.

On the cardiovascular system, obesity accelerates atherosclerosis progression, inducing pathophysiological changes that are detectable already from young adults. Endothelial dysfunction is one of the earliest vascular alterations observed in obesity. In this study, we aimed to determine endothelial functions and carotid intima-media thickness in patients with obesity without overt cardiovascular disease. The study was conducted with 112 individuals with obesity without overt cardiovascular disease and any chronical diseases (BMI>30 kg/m2) (84 female, 28 male, mean age: 46.3±11.2 years) and 49 healthy individuals with no diseases (33 female, 16 male, mean age: 44.6±10.2 years). All patients were examined for endothelial functions by the flow-mediated dilatation (FMD) method and carotid intima-media thicknesses (CIMT). All measurements were performed by the same imaging specialist, averaging 3 different measurements. In addition to the body mass index and waist circumference visceral adiposity index (VAI) and triponderal mass index (TPI) also calculated. The percentage of FMD obtained by brachial artery ultrasound was significantly lower, visceral adipose tissue, perirenal adipose tissue thicknesses measured by abdominal ultrasound and CIMT were significantly thicker in Group 1 compared to Group 2. FMD had a negative significant correlation with body mass index, visceral adipose tissue thickness, perirenal adipose tissue thickness, and waist and hip circumferences, and carotid intima-media thickness and CIMT had a significant correlation with visceral adipose tissue thickness, perirenal adipose tissue thickness, VAI, TPI and waist, hip circumferences. Individuals with obesity have impaired endothelial functions and greater carotid intima-media thicknesses compared to healthy individuals. This impairment in endothelial functions is proportional to the amount of visceral and perirenal fat accumulation. Parameterss reflecting visceral fat distribution such as VAI and TPI are also related with these impairment.

A contemporary view of the mechanisms of formation of new contaminants in vegetable oils – esters of monochloropropanediols and high molecular weight carboxylic acids

Monochloropropanediols (MCPD) and their esters with high molecular weight carboxylic (fatty) acids are new types of technological contaminants present in refined deodorized oils and other foods.The development of measures to minimize the content of MCPD esters in vegetable oils, which are an important component of various food systems, is a priority direction of modern foreign and domestic research in the field of ensuring food safety. The effectiveness of such measures should be based on ideas about the mechanisms of formation of MCPD esters and the identification of their predecessors (precursors) present in the lipid complex of oil seeds, as well as those formed during their processing.Systematized statistically reliable scientific knowledge about the mechanisms of formation of MCPD esters and their derivatives is still missing. Presumably, the formation of MCPDs and their esters with fatty acids should correlate with the presence of chlorine compounds in oilseed raw materials and in the oil itself; chlorine-containing pesticides and biologically active substances (fertilizers) used in agricultural production are one of the sources.Thus, the goal of the research is a systematic and comprehensive synthesis of ideas about precursors and mechanisms of formation of MCPD esters.As a result of analysis and synthesis of literature sources, it has been established that the main precursors of MCPD esters are DAG, TAG and various chlorine-containing compounds. The main factors influencing the rate of formation and amount of accumulation of MCPD esters are high temperatures (more than 120°C), duration of heat treatment, the presence of free fatty acids, antioxidants and moisture. Different chlorine compounds appear to have different activities in the reactions leading to the formation of MCPD esters. The presence of antioxidants in lipid systems can inhibit the formation of 3-MCPD ester, and the effectiveness of the process is determined by the type of antioxidant. According to the degree of increase in inhibitory ability, the most applicable antioxidants in lipid systems can be ranked as follows: α-tocopherol, BOA, BOT, AP, PG and TBHQ. The role of the composition of fatty acids in the formation of MCPD esters still remains unclear; however, there is indirect evidence indicating the influence of the composition of fatty acids on the level of accumulation of MCPD esters, which determines the feasibility of an in-depth study of this issue.

CORN FOR GRAIN IN CONTINUOUS GROWING UNDER DIFFERENT FERTILIZER SYSTEMS AND WEATHER CONDITIONS

On the experimental field of the Poltava State Agricultural Experimental Station named after M. I. Vavilov of the The Institute of Pig Breeding and Agro-Industrial Production of the National Academy of Agrarian Sciences of Ukraine, during 1984-2023, on typical chernozem in conditions of unstable moisture of the Left-Bank Forest-Steppe, were conducted researches to study the continuous growing of corn for grain and its productivity. Precipitation is the leading factor in providing corn with available moisture in this region. Different hydrothermal conditions, especially the amount of moisture during the agricultural year and the vegetation, led to sharp fluctuations in grain yield over the years, which occurred in a wide range. The analysis of the average values ​​of corn productivity for each 10 consecutive years of conducting these studies made it possible to establish that during the first decade of observations, the dynamics of this indicator in subsequent periods took place in the paradigm of its growth, albeit at a different level. Maize grain yield and its correlation with different weather conditions and fertilization systems, regardless of the replacement of maize hybrids, ranged from direct to inverse and from low to high. Long-term application of different doses and ratios of organic and mineral fertilizers contributed to changes in both quantitative and qualitative indicators of humus in the soil. It was determined that the content of total carbon in the fertilized areas was higher by 3,0 and 3,3 relative percent compared to the unfertilized ones. Special attention was paid to the ratio between humic and fulvic acids, which directly depends on the fertilization system. The different effect of this agrotechnical measure on the amount of humus accumulation in the soil is shown. Thus, in areas without fertilizers (control), its accumulation occurs at the level of an average indicator and a weak effect of organic acids on the mineral part of the soil, while under the organo-mineral fertilization system, this process takes place intensively, and the mineral part remains almost unchanged.

Larval occurrence prediction of Dendrolimus punctatus based on fuzzy integrated evaluation method

AbstractTo improve the prediction accuracy of Dendrolimus punctatus larvae of overwintering, first and second generation and to provide a scientific basis for effective prevention and control of D. punctatus, six mathematical models of fuzzy comprehensive evaluation method were used to predict accumulative amount with the data of 1989–2014. The cumulative amount for the overwintering and the first generation was predicted in 2015, 2016, 2017, and 2018. The forecasted results of six models were all level 1, which was consistent with the actual results. Five forecasted results of six models and the actual results were level 1. The actual accumulative amount of D. punctatus for the second generation was level 1 in 2015, 2016, and 2017, which were consistent with the six models' forecasted results.

Dendrimers as Drug Delivery Platforms of the Mechanisms, Benefits, and Use in Anti-Pain: Systematice Literature Review

Background: Efforts to reduce the side effects of long-term NSAID use have prompted exploration of transdermal drug delivery, which offers advantages such as avoiding first-pass metabolism, reducing pain, allowing sustained drug release, and improving patient compliance. Dendrimers, specifically polyamidoamine (PAMAM), are attracting attention for their potential in drug delivery. PAMAM has a three-dimensional structure of monodisperses with high amino group density and internal voids suitable for drug encapsulation. Methods: This research method uses the literature review method which begins with the collection of journals to be reviewed. The article search was conducted on the Pubmed database. Researchers searched with the keyword dendrimer. The two main synthesis methods are convergent and divergent dendrimers. Results: : Drug molecules can be trapped in dendrimers through hydrogen, hydrophobic, or electrostatic bonds. The advantages of dendrimers include improved solubility, permeability, and targeted drug delivery, while the disadvantages are high cost, toxicity and aggregation. This system has been used in several pain medications. Conclusions: Dendrimers are macrostructures with unique characteristics such as nanoscopic size, multifunctional surface, and high branching, making them ideal as drug delivery systems. Ketoprofen with PAMAM-type dendrimers showed that PAMAM dendrimers could significantly increase the accumulative amount of permeation of both drugs after 24 hours, compared with the drug suspension without PAMAM dendrimers. Dendrimers are multi-branched monodisperse macrostructures, derived from Greek.

Mandelonitrile lyase MDL2-mediated regulation of seed amygdalin and oil accumulation of Prunus Sibirica

BackgroundThe Prunus sibirica seeds with rich oils has great utilization, but contain amygdalin that can be hydrolyzed to release toxic HCN. Thus, how to effectively reduce seed amygdalin content of P. sibirica is an interesting question. Mandelonitrile is known as one key intermediate of amygdalin metabolism, but which mandelonitrile lyase (MDL) family member essential for its dissociation destined to low amygdalin accumulation in P. sibirica seeds still remains enigmatic. An integration of our recent 454 RNA-seq data, amygdalin and mandelonitrile content detection, qRT-PCR analysis and function determination is described as a critical attempt to determine key MDL and to highlight its function in governing mandelonitrile catabolism with low amygdalin accumulation in Prunus sibirica seeds for better developing edible oil and biodiesel in China.ResultsTo identify key MDL and to unravel its function in governing seed mandelonitrile catabolism with low amygdalin accumulation in P. sibirica. Global identification of mandelonitrile catabolism-associated MDLs, integrated with the across-accessions/developing stages association of accumulative amount of amygdalin and mandelonitrile with transcriptional level of MDLs was performed on P. sibirica seeds of 5 accessions to determine crucial MDL2 for seed mandelonitrile catabolism of P. sibirica. MDL2 gene was cloned from the seeds of P. sibirica, and yeast eukaryotic expression revealed an ability of MDL2 to specifically catalyze the dissociation of mandelonitrile with the ideal values of Km (0.22 mM) and Vmax (178.57 U/mg). A combination of overexpression and mutation was conducted in Arabidopsis. Overexpression of PsMDL2 decreased seed mandelonitrile content with an increase of oil accumulation, upregulated transcript of mandelonitrile metabolic enzymes and oil synthesis enzymes (involving FA biosynthesis and TAG assembly), but exhibited an opposite situation in mdl2 mutant, revealing a role of PsMDL2-mediated regulation in seed amygdalin and oil biosynthesis. The PsMDL2 gene has shown as key molecular target for bioengineering high seed oil production with low amygdalin in oilseed plants.ConclusionsThis work presents the first integrated assay of genome-wide identification of mandelonitrile catabolism-related MDLs and the comparative association of transcriptional level of MDLs with accumulative amount of amygdalin and mandelonitrile in the seeds across different germplasms and developmental periods of P. sibirica to determine MDL2 for mandelonitrile dissociation, and an effective combination of PsMDL2 expression and mutation, oil and mandelonitrile content detection and qRT-PCR assay was performed to unravel a mechanism of PsMDL2 for controlling amygdalin and oil production in P. sibirica seeds. These findings could offer new bioengineering strategy for high oil production with low amygdalin in oil plants.

Embedded catalysts prepared by grinding crystallization and their catalytic performance in dry reforming of methane: Domain-limited nanostructures for high activity and stability

Dry reforming of methane (DRM) takes advantage of greenhouse gases (CH4 and CO2) and mitigates their eco-environmental consequences. However, the biggest obstacle for DRM reactions has been the design of catalysts with high resistance to sintering and carbon deposition. In this paper, the embedded bimetallic nickel-cobalt@ silicalite-1 (S1) catalysts (NiCo@S1), with high resistance to sintering and lower carbon deposition, were prepared by grinding crystallization method. The physicochemical characterizations demonstrated that the embedded catalyst for NiCo@S1 could effectively control the size and dispersion of the active metal Ni–Co in the catalyst compared to the supported catalysts for 10Ni/SiO2 and 10Ni/S1, which further enhance the interaction between the active metal phase and the carrier S1, and greatly minimize the amount of carbon accumulation on the catalysts. After being subjected to a reaction at 700 °C for 6 h, the 10Ni2.5Co@S1 exhibited a significant performance improvement. The instantaneous conversion rate of CH4 increased from 63.17% to 86.39% (an increase of 23.22%), and the instantaneous conversion rate of CO2 rose from 70.20% to 88.62% (an increase of 18.42%). Additionally, carbon deposition reduced from 14.58% to 0.82% (a decrease of 13.76%). Therefore, the conceptual design provided a new perspective for designing Ni-based dry reforming catalysts.

Ultrahigh-Speed 3D DNA Walker with Dual Self-Protected DNAzymes for Ultrasensitive Fluorescence Detection and Intracellular Imaging of microRNA.

Herein, a dual self-protected DNAzyme-based 3D DNA walker (dSPD walker), composed of activated dual self-protected walking particles (ac-dSPWPs) and track particles (TPs), was constructed for ultrasensitive and ultrahigh-speed fluorescence detection and imaging of microRNAs (miRNAs) in living cells. Impressively, compared with the defect that "one" target miRNA only initiates "one" walking arm of the conventional single self-protected DNAzyme walker, the dSPD walker benefits from the secondary amplification and spatial confinement effect and could guide "one" target miRNA to generate "n" secondary targets, thereby initiating "n" nearby walking strands immediately, realizing the initial rate over one-magnitude-order faster than that of the conventional one. Moreover, in the process of relative motion between ac-dSPWPs and TPs, the ac-dSPWPs could cleave multiple substrate strands simultaneously to speed up movement and reduce the derailment rate, as well as combine with successive TPs to facilitate a large amount of continuous signal accumulation, achieving an ultrafast detection of miRNA-221 within 10 min in vitro and high sensitivity with a low detection limit of 0.84 pM. In addition, the DNA nanospheres obtained by the rolling circle amplification reaction can capture the Cy5 fluorescence dispersed in liquids, which achieves the high-contrast imaging of miRNA-221, resulting in further ultrasensitive imaging of miRNA-221 in cancer cells. The proposed strategy has made a bold innovation in the rapid and sensitive detection as well as intracellular imaging of low-abundance biomarkers, offering promising application in early diagnosis and relevant research of cancer and tumors.

Optimizing Promoters and Subcellular Localization for Constitutive Transgene Expression in Marchantia polymorpha.

Marchantia polymorpha has become an important model system for comparative studies and synthetic biology. The systematic characterization of genetic elements would make heterologous gene expression more predictable in this test bed for gene circuit assembly and bioproduction. Yet, the toolbox of genetic parts for Marchantia includes only a few constitutive promoters that need benchmarking to assess their utility. We compared the expression patterns of previously characterized and new constitutive promoters. We found that driving expression with the double enhancer version of the cauliflower mosaic virus 35S promoter (pro35S × 2) provided the highest yield of proteins, although it also inhibits the growth of transformants. In contrast, promoters derived from the Marchantia genes for ETHYLENE RESPONSE FACTOR 1 and the CLASS II HOMEODOMAIN-LEUCINE ZIPPER protein drove expression to higher levels across all tissues without a growth penalty and can provide intermediate levels of gene expression. In addition, we showed that the cytosol is the best subcellular compartment to target heterologous proteins for higher levels of expression without a significant growth burden. To demonstrate the potential of these promoters in Marchantia, we expressed RUBY, a polycistronic betalain synthesis cassette linked by P2A sequences, to demonstrate coordinated expression of metabolic enzymes. A heat-shock-inducible promoter was used to further mitigate growth burdens associated with high amounts of betalain accumulation. We have expanded the existing tool kit for gene expression in Marchantia and provided new resources for the Marchantia research community.

Inhibition characteristics of pollution accumulation in high-voltage direct current conductors with XLPE and PVC film

Abstract The inhibition of pollution accumulation on the ground equipment by cross-linked polyethylene (XLPE) and polyvinyl chloride (PVC) as coating materials was investigated, and the effects of discharge voltage, power supply polarity, and the material properties of ground equipment on the characteristics of dirt accumulation were discussed. When the voltage was greater than 40 kV, the pollution accumulation of the sampling plate of the PVC-coated conductor was higher than that of the XLPE-coated conductor. Whether it was PVC or XLPE coated wire, the amount of pollution accumulation under negative polarity power was greater than that under positive polarity power. When the ground equipment was made of aluminum sheets, the amount of pollution accumulated was greater than that of the PTFE. As the film thickness increased, the amount of pollution on the aluminum fouling film decreased continuously.

Experimental evaluation of performance parameters of a domestic freezer under door opening conditions

The current study focuses on a comprehensive experimental assessment of the cooling system parameters of a domestic-type upright freezer that is equipped with shelves and test packages under periodic door opening conditions, referring to real user scenarios. The influence of varying ambient relative humidity levels as well as the load state of the cabinet are taken into account. The study reveals that both heat and moisture have a crucial effect on the average air temperature inside the cabinet, the temperature of the walls, the transient evaporation and condensation pressure behaviour of the refrigerant, as well as the compressor power, the total operation ratio of the compressor, and the overall amount of energy consumption. The specific performance experiments are conducted at 32 °C ambient and 35 %, 65 %, and 85 % relative humidity conditions with unloaded, half-loaded, and full-loaded cases. The obtained data and results are evaluated between frosting, influenced by altering ambient humidity levels, and cabinet load state under periodic door openings where the variation in energy consumption is clearly measured. Consequently, the amounts of frost accumulation in the cabinet under half and full loaded conditions at 32 °C an 85 % relative humidity conditions are observed to be about 220 g and 270 g, respectively, followed by regular door openings and closings which result in a raise in total energy consumption rate of 7.6 % and 11.2 % compared to the period before the door opening. The frost accumulation in the full loaded condition at 32 °C and 85 % RH is found to be 2.4 times greater than the unloaded condition with a relative humidity (RH) of 35 % whereas the energy consumption is 3.7 times higher.

Syn-tasiRnas targeting the coat protein of potato virus Y confer antiviral resistance in Nicotiana benthamiana

ABSTRACT Trans-acting small interfering RNAs (tasiRNAs) are 21-nt phased (phased siRNAs) resulting from successive DCL-catalyzed processing from the end of a double-stranded RNA substrate originating from the RDR of an AGO-catalyzed cleaved RNA at a micro RNA target site. Plant tasiRNAs have been synthesized to produce synthetic tasiRNAs (syn-tasiRNAs) targeting viral RNAs that confer viral resistance. In this study, we engineered syn-tasiRNAs to target potato virus Y (PVY) infection by replacing five native siRNAs of TAS1c with 210-bp fragments from the coat protein (CP) region of the PVY genome. The results showed that the transient expression of syn-tasiR-CPpvy2 in Nicotiana benthamiana (N. benthamiana) plants conferred antiviral resistance, supported by the absence of PVY infection symptoms and viral accumulation. This indicated that syn-tasiR-CPpvy2 successfully targeted and silenced the PVY CP gene, effectively inhibiting viral infection. syn-tasiR-CPpvy1 displayed attenuated symptoms and decreased viral accumulation in these plants However, severe symptoms of PVY infection and a similar amount of viral accumulation as the control were observed in plants expressing syn-tasiR-CPpvy3. syn-tasiR-CPpvy/pvx, which targets both PVY and potato virus X (PVX), was engineered using a single precursor. After the transient expression of syn-tasiR-CPpvy/pvx3 and syn-tasiR-CPpvy/pvx5 in N. benthamiana, the plants were resistant to both PVY and PVX. These results suggested that engineered syn-tasiRNAs could not only specifically induce antiviral resistance against one target virus but could also be designed for multi-targeted silencing of different viruses, thereby preventing complex virus infection in plants.

Analisis Efisiensi Waktu Siklus Untuk Meningkatkan Produktivitas Dengan Line Balancing Pada Proses Pengemasan Produksi Obat Diabetes di PT.OPQ

Operation Excelence is needed at manufacturing process in order to achive optimum cost. One of method is using line balancing. It work by increasing efficiency and remove bottole neck at workstation.. The purpose of this research is to increase productivity in the work path of diabetes drug production line, it means that all work stations can achieve optimum output, eliminate or reduce material accumulation by identifying line balances at work stations. By using the Line Balancing method. The method used is the Ranked Position Weight (RPW) heuristic method. As result productivity was increasing, with line efficiency from 58.36% to 80.25%, balance delay from 41.64% to 19.75%, a decrease in the amount of material accumulation between work stations on the diabetes drug production trajectory from 25 - 1175 doos / hour to 4 - 174 doos / hour, and eliminating the bottleneck on the trajectory of diabetes drug production.

A non-canonical role of ELN protects from cellular senescence by limiting iron-dependent regulation of gene expression

The ELN gene encodes tropoelastin which is used to generate elastic fibers that insure proper tissue elasticity. Decreased amounts of elastic fibers and/or accumulation of bioactive products of their cleavage, named elastokines, are thought to contribute to aging. Cellular senescence, characterized by a stable proliferation arrest and by the senescence-associated secretory phenotype (SASP), increases with aging, fostering the onset and progression of age-related diseases and overall aging, and has so far never been linked with elastin. Here, we identified that decrease in ELN either by siRNA in normal human fibroblasts or by knockout in mouse embryonic fibroblasts results in premature senescence. Surprisingly this effect is independent of elastic fiber degradation or elastokines production, but it relies on the rapid increase in HMOX1 after ELN downregulation. Moreover, the induction of HMOX1 depends on p53 and NRF2 transcription factors, and leads to an increase in iron, further mediating ELN downregulation-induced senescence. Screening of iron-dependent DNA and histones demethylases revealed a role for histone PHF8 demethylase in mediating ELN downregulation-induced senescence. Collectively, these results unveil a role for ELN in protecting cells from cellular senescence through a non-canonical mechanism involving a ROS/HMOX1/iron accumulation/PHF8 histone demethylase pathway reprogramming gene expression towards a senescence program.

Phenanthrene metabolism in Panicum miliaceum: anatomical adaptations, degradation pathway, and computational analysis of a dioxygenase enzyme.

Polycyclic aromatic compounds (PAHs) are persistent organic pollutants of environmental concern due to their potential impacts on food chain, with plants being particularly vulnerable. While plants can uptake, transport, and transform PAHs, the precise mechanisms underlying their localization and degradation are not fully understood. Here, a cultivation experiment conducted with Panicum miliaceum exposed different concentrations of phenanthrene (PHE). Intermediate PHE degradation compounds were identified via GC-MS analysis, leading to the proposal of a phytodegradation pathway featuring three significant benzene ring cleavage steps. Our results showed that P. miliaceum exhibited the ability to effectively degrade high levels of PHE, resulting in the production of various intermediate products through several chemical changes. Examination of the localization and anatomical characteristics revealed structural alterations linked to PHE stress, with an observed enhancement in PHE accumulation density in both roots and shoots as treatment levels increased. Following a 2-week aging period, a decrease in the amount of PHE accumulation was observed, along with a change in its localization. Bioinformatics analysis of the P. miliaceum 2-oxoglutarate-dependent dioxygenase (2-ODD) DAO-like protein revealed a 299 amino acid structure with two highly conserved domains, namely 2OG-FeII_Oxy and DIOX_N. Molecular docking analysis aligned with experimental results, strongly affirming the potential link and direct action of 2-ODD DAO-like protein with PHE. Our study highlights P. miliaceum capacity for PAHs degradation and elucidates the mechanisms behind enhanced degradation efficiency. By integrating experimental evidence with bioinformatics analysis, we offer valuable insights into the potential applications of plant-based remediation strategies for PAHs-contaminated environments.

Study on Slump and Compressive Strength of Gangue Based on Aggregate Size Gradation

In order to solve the ecological environment pollution caused by a large amount of coal gangue accumulation and the problems of poor conveying performance and low support strength of paste filling materials. Based on the standard slump test and uniaxial compressive strength test, the slump and compressive strength (post-coagulation) of gangue paste under different aggregate size aggregations were obtained through orthogonal experiments in this study. The results show that the slump of the gangue filling paste with four types of aggregate size gradations is negatively correlated with the mass fraction, and the slump of the filling paste with a coarser aggregate content is more obviously affected by the mass fraction The increase of fine aggregate content has a significant impact on the compressive strength of the filling paste, which shows a trend of first increasing and then decreasing. When the mixing ratio of coarse aggregate and fine aggregate is 5:5, the compressive strength of the paste reaches the best. In addition, different proportions of aggregate mixing cause the filling paste to form different skeleton structures, including the skeleton void structure at 7:3 or 6:4, the skeleton dense structure at 5:5, and the skeleton suspension structure at 4:6, which are decisive for the final performance of the paste. By analyzing the experimental results of the compressive strength and slump of the gangue filling paste, it was found that the relationship between the compressive strength and slump of the gangue filling paste is a power index function. Through data fitting, it was found that the regression coefficient of the fitting function is no less than 0.97, and the fitting effect is good for evaluating the strength of the filling paste under the aggregate size grading.

Predicting reservoir sedimentation using multilayer perceptron – Artificial neural network model with measured and forecasted hydrometeorological data in Gibe-III reservoir, Omo-Gibe River basin, Ethiopia

The estimation and prediction of the amount of sediment accumulated in reservoirs are imperative for sustainable reservoir sedimentation planning and management and to minimize reservoir storage capacity loss. The main objective of this study was to estimate and predict reservoir sedimentation using multilayer perceptron–artificial neural network (MLP-ANN) and random forest regressor (RFR) models in the Gibe-III reservoir, Omo-Gibe River basin. The hydrological and meteorological parameters considered for the estimation and prediction of reservoir sedimentation include annual rainfall, annual water inflow, minimum reservoir level, and reservoir storage capacity. The MLP-ANN and RFR models were employed to estimate and predict the amount of sediment accumulated in the Gibe-III reservoir using time series data from 2014 to 2022. ANN-architecture N4-100-100-1 with a coefficient of determination (R2) of 0.97 for the (80, 20) train-test approach was chosen because it showed better performance both in training and testing (validation) the model. The MLP-ANN and RFR models' performance evaluation was conducted using MAE, MSE, RMSE, and R2. The models’ evaluation result revealed that the MLP-ANN model outperformed the RFR model. Regarding the train data simulation of MLP-ANN and RFR shown R2 (0.99) and RMSE (0.77); and R2 (0.97) and RMSE (1.80), respectively. On the other hand, the test data simulation of MLP-ANN and RFR demonstrated R2 (0.98) and RMSE (1.32); and R2 (0.96) and RMSE (2.64), respectively. The MLP-ANN model simulation output indicates that the amount of sediment accumulation in the Gibe-III reservoir will increase in the future, reaching 110 MT in 2030–2031, 130 MT in 2050–2051, and above 137 MTin 2071–2072.