SummaryTo estimate a suitable index for harvesting time of white sapote (Casimiroa edulis Llave & Lex.), we investigated the changes in fruit and seed size, skin colour, seed colour, total soluble solids (TSS), sugars and polyphenol contents during fruit development in cv. ‘Cuccio’ pollinated at different times. Fruit growth showed a sigmoid curve and, therefore, was not a suitable index for maturity. Accumulated temperature (AT), which is a simple sum of hourly temperatures, or days after pollination (DAP) could be used as an index. An AT of approx. 120 103 degree hours (°C h) or 212 DAP were required for maturity of cv. ‘Cuccio’ fruit. There was a strong correlation between TSS content and percentage dry matter (DM) in pulp; therefore, a %DM ≥ 18.0% could also be used as an index of fruit maturity.Fruit skin colour, specifically the CIE a* value on the sun-exposed side, the b* value on the shaded side or seed colour were also good indicators of fruit maturity. Measurements of fruit skin colour using a colorimeter provides a convenient non-destructive detection method for fruit maturity.