Accelerated Shelf Life Testing Method Research Articles

Shelf-life Estimation of Dried Chili in Vacuum Packaging

This research aimed to build a model for estimating the shelf life of dried chilies in vacuum packaging stored at any storage temperature. The shelf-life estimation of dried red chilies was carried out based on the change rate of dried chilies quality parameters using the ASLT (Accelerated Shelf-Life Testing) method with the Arrhenius approach. The shelf-life estimation using the ASLT method was carried out by conditioning the storage room, which could accelerate the degradation reaction, stored at temperatures above room temperature. Prior to storage, red chilies were dried until their moisture content reaches 10%. Then it was packaged in vacuum packaging made of PE (polyethylene) plastic and stored at 35°C. During the storage period, the observation was conducted on some quality parameters, namely moisture content, color, and vitamin C content. The same experiments were also carried out at 40°C, 45°C, and 50°C to obtain different rate constant values. The shelf-life estimation results from the Arrhenius equation model were selected based on the critical parameter, in this study the critical parameter was the decrease of vitamin C content. The shelf life of dried red chilies that were packed in vacuum stored at room temperature (25°C) could maintain its quality up to 365 days. Keywords: Arrhenius, ASLT, Red chili, Vacuum packaging, Shelf life,

Estimation of Shelf Life of Pegagan Tea (Centella asiatica (L) Urban) using Accelerated Shelf Life Testing (ASLT) Method with Arrhenius Model

This research aimed to evaluate the shelf life of pegagan (Centella asiatica (L) Urban) tea using the Accelerated Shelf Life Testing (ASLT) method by applying the Arrhenius Model since its widely known for its various health benefits, including its ability to enhance cognitive function and exhibit antioxidant effects. The ASLT method was employed to accelerate the chemical and physical changes in the product, thereby enabling a shorter estimation of shelf life compared to conventional methods. In this research, samples of pegagan tea were packaged in paper sacks, aluminum foil, and vacuum-sealed plastic and stored at temperatures of 15, 30, and 45 °C for 28 days, with observations conducted every 7 days. The Arrhenius Model was utilized to predict the rate of chemical reactions in the product, taking into account the temperature's influence on these changes. The data were analyzed using Analysis of Variance (ANOVA) at a 5% confidence level. If the ANOVA results showed significant differences, Duncan Multiple Range Test (DMRT) was further conducted. The results of the observations indicated that storage temperature and packaging type significantly influenced the yield value and pH value, but had no significant effect on the moisture content of pegagan tea. The estimated shelf life of pegagan tea for 28 days using paper sack packaging at temperatures of 15, 30, and 45 °C was 173, 616, and 195 days, respectively. For aluminum foil packaging, the estimated shelf life was 189, 661, and 205 days at the same temperatures. As for vacuum-sealed plastic packaging, the estimated shelf life was 551, 192, and 597 days at the respective temperatures. The findings of this research are expected to provide valuable information for manufacturers and consumers regarding the shelf life of pegagan Tea. Additionally, the research aims to offer a scientific basis for appropriate packaging and storage practices to better maintain the product's quality.

Sensory Quality Analysis (Texture) and Moisture Content to Predict the Shelf Life of Bakpia Filled Seaweed (Eucheuma cottonii)

Aims: Bakpia is a snack made from a mixture of mung beans and sugar wrapped in pastry and baked. Bakpia is a popular light snack consumed by the Indonesian community. This research aims to determine the shelf life of seaweed-filled bakpia and regular bakpia using the Accelerated Shelf Life Test (ASLT) method.
 Place and Duration: The study was conducted at the Chemistry Laboratory of Pangkep State Polytechnic of Agriculture and the Chemistry Laboratory of Makassar Ministry of Labaoratory Industry from February to May 2023.
 Study Design: The research design involved using the ASLT method to analyze the minimum quality limit at which the bakpia cookies product starts to be rejected by consumers. Samples of the product were obtained from a local mini-market that sells bakpia. The parameters observed were the initial and final moisture content during storage at 15°C, 30°C, and 45°C, as well as the texture before and after the bakpia cookies product was rejected by consumers.
 Results: Estimating the shelf life of seaweed bakpia tausa and bakpia tausa using the ASLT method can use a degradation reaction approach (absorption of water content) as a critical parameter. The kinetic reactions of degradation (absorption of water content) of seaweed bakpia tausa and bakpia tausa follow the first order reaction. The shelf life of seaweed bakpia tausa and bakpia tausa uses the ASLT method through a different approach to the critical parameter of water content for each product. At storage temperatures between 15°C and 45°C, the shelf life of bakpia tausa is longer than that of seaweed bakpia tausa. The average shelf life of seaweed bakpia tausar at 30°C is 59.5 days while bakpia lausa is 64.8 days (more than two months).
 Conclutions: The shelf life of seaweed bakpia tausa and bakpia tausa uses the ASLT method through a different approach to the critical parameter of water content for each product. At storage temperatures between 15°C and 45°C, the shelf life of bakpia tausa is longer than that of seaweed bakpia tausa. The average shelf life of seaweed bakpia tausar at 30°C is 59.5 days while bakpia lausa is 64.8 days (more than two months).

The effect of natural antioxidant from clove (Syzygium aromaticum L.) on the oxidative stability of crude oil produced by the traditional wet rendering process of coconut (Cocos nucifera L.)

Coconut oil produced by the traditional wet rendering process may contain free fatty acids and water which lead to rancidity and shorter shelf life. Previous studies proved that cloves (Syzygium aromaticum L.), as a natural source of antioxidant eugenol, can increase coconut oil oxidation stability. This research aimed to investigate the effects of clove addition during the wet rendering process on the Peroxide Value of coconut oil and to estimate shelf life using Accelerated Shelf Life Testing (ASLT) and Artificial Neural Network (ANN). Clove powder was added into coconut milk prior to oil rendering or into coconut oil, followed by oil storage at 28, 63, and 100°C. ASLT method predicted that adding 1 g clove/kg coconut milk and 5 g clove/kg coconut oil gave the best result by improving oil shelf life from approximately 11 days (control) to 14 days, while the ANN method predicted that adding 10 g clove/kg coconut milk gave the best result by improving estimated oil shelf life to 47 days at 28oC. Coconut oil produced from clovetreated coconut milk had the best oxidative stability during storage. Activation energies and activation enthalpies of coconut oil oxidation increased due to clove addition and therefore, improved stability during storage.

Estimation of vegetable leather shelf life from a combination of beluntas leaves (Pluchea indica L.) and seaweed (Eucheuma cottonii) with various types of packaging using the ASLT (accelerated shelf life testing) method of the arrhenius model

Beluntas leaves vegetable leather is a new processed product, so its shelf life is currently unknown. This research utilised the ASLT (Accelerated Shelf Life Test) Arrhenius model to assess the shelf life of vegetable leather in three different forms of packaging, including aluminum foil, combination (aluminum foil-polyethylene), and polypropylene. The research includes two replications and a descriptive design. Three different types of packing were used to store vegetable leather for a month at oven temperatures of 30°C, 35°C, and 40°C (28 days). Every week, on days 0, 7, 14, 21, and 28, observations were made about the moisture content and sensory factors (texture, color, and aroma). The shelf life of vegetable leather was calculated using the parameter data using the Arrhenius model ASLT method with Microsoft Excel 2010. The results indicated that vegetable leather beluntas leaves packaged in aluminum foil had a shelf life of 179.53 days (5 months, 27 days), while in combination packaging (aluminum foil-polyethylene), they had a shelf life of 44.20 days (1 month, 13 days). In polypropylene packaging, the product had a shelf life of 35.94 days (1 month and four days) according to order 1 of the Arrhenius model ASLT method.

The Shelf-Life Prediction of Black Garlic Chili Sauce and “Cahyo” Garlic Chili Sauce with Accelerated Shelf-Life Testing (ASLT) Method Based on The Arrhenius Model

Sambal is often found in various menu variants served in the Indonesian food industry; this is a supporting factor for creating bottled chili sauce. In this research, fermented black garlic chili sauce was made with black garlic as raw material to provide added value in the form of antibacterial and antioxidant compounds, as well as giving a novelty value to the product as well as a differentiator in the elements of color, aroma, consistency, and taste of the product. The final product consumers receive is compared to "Cahyo" garlic chili sauce produced by PT. Deltasari Indah Restaurant. Sambal contains oil/fat, which is quickly rancid, thus affecting the element of consumer acceptance. Therefore, it is necessary to predict the shelf life of bottled chili products. This research aims to predict the shelf life of the black garlic chili sauce and “Cahyo” garlic chili sauce products in bottles produced by PT. Deltasari Indah Restaurant with the ASLT method, the Arrhenius model approach; Knowing the difference in the shelf life of bottled chili products stored at different temperature conditions; and determining the proper temperature and storage conditions to get the maximum shelf life for packaged chili products. This study uses the Accelerated Shelf-Life Testing method with the Arrhenius model. Based on the pH value parameter, the shelf life of black garlic chili sauce was 33 days, and “Cahyo” garlic chili sauce was 45 days. These two results are the results of the shelf life obtained from calculations at 20˚C and 25˚C then the average value is taken.

Pendugaan Umur Simpan Sambal Gami Dikemas dalam Jar Polyethylene Terephthalate (PET)

<em>“Sambal gami” is a typical sauce from Bontang, East Kalimantan, but there has been no research estimating its shelf life. This study aimed to determine the rate of deterioration and estimate the shelf life of “sambal gami” using the Accelerated Shelf Life Testing method based on the Arrhenius approach. “Sambal” was packaged in a Polyethylene Terephthalate plastic jar and stored at 25°C, 30°C and 40°C. Parameters observed during storage Free Fatty Acid (FFA) levels and the number of microbes. The results showed the smallest activation energy value to activate the shelf life of sambal gami, the parameter of FFA levels, namely in the zero-order reaction with linear regression y = 0.3404x + 1.5304, R2 value = 0.9796, quality constant of 0.336 and has shelf life of 6 days at 25°C. Based on the parameters of the total number of microbes that occur in the first-order reaction, with linear regression y = 0.2491x + 7.9356, R2 value = 0.9854, the quality degradation constant is 0.263 and has a shelf life of 5 days at 25°C. The headspace obtained on the package is 40.8%. An excellent standard index for headspace is 10% of pack height.</em>

Shelf Life of Combination Nori Sargassum sp. and Eucheuma spinosum with Alumunium Foil Packaging Based on the Accelerated Shelf Life Test Method

The purpose of this research was to determine the shelf life of Combination Nori Sargassum sp. and Eucheuma spinosum with the Accelerated Shelf Life Test (ASLT) method Arrhenius Model using Alumunium Foil packaging. This research was conducted at the Laboratory of Fisheries Product Processing, Faculty of Fisheries and Marine Sciences, Padjadjaran University from October to November 2020. Sargassum sp. is a genus of brown algae (Phaeophyta) used as in the food industry and non-food industry. Sargassum sp. cannot form nori sushi sheets because it lack carageenan. Eucheuma spinosum is a genus of red algae (Rhodophyta) used to complement the lack of carageenan in Sargassum sp. Alumunium foil is a metalized plastic package composed of hermetic, flexible and opaque metal that has high protection againts light, water vapor and gas. The determination of the shelf life of the ASLT method is carried out using parameters of environmental conditions that can accelerate the product quality degradation by storing the product at high risk temperature. The observation used in determining the shelf life of Combination Nori Sargassum sp. and Eucheuma spinosum is a sensory test and water content test with 35 days in the storage at a temperature of 25oC and 35oC. The calculation results of the Arrhenius model used texture parameter as critical parameters that had the lowest Activation energy (Ea) in determining the shelf life of combination nori which packaged with alumunium foil. The results showed the value of texture parameters based on the order 0 reaction with the Arrhenius plot Ln K = -2.8019, 1/T = 97.596 and Ea = 193.92. The shelf life of combination nori Sargassum sp. and Eucheuma spinosum that stored at room temperature (25oC) was around 137 days.

Accelerated shelf life test method with arrhenius approach for shelf life estimation of tongkol ‘euthynnus affinis’ balado in cans

Many people in Indonesia consumed tongkol fish by processing it into balado tongkol. The process carried out to extend the shelf life of the product is by packing it in cans. Information related to shelf life was needed for canned food, so that it can guarantee that the food was still good for consumption. This study aims to determine the shelf life of balado tongkol in cans from TEFA Fish Canning with the Arrhenius method. Arrhenius is the method of estimating shelf life used was ASLT (Accelerated Shelf Life Test). The ASLT method requires a relatively short testing time with an acceptable level of accuracy. This research was conducted at the TEFA Fish Canning and Food Processing Laboratory of Politeknik Negeri Jember in March – July 2021. Estimating the shelf life used the reaction approach that increases the TBA number as a critical parameter. The kinetic reaction of increasing the TBA number in canned balado tongkol followed the ordo 0 reaction. The results showed that the shelf life of balado tongkol in cans at room temperature was 24,6 weeks.

Shelf life prediction of straw mushrooms (Volvariella volvacea) based food enhancer using accelerated shelf life testing method

Food shelf life is related to food safety since consumers need to be informed an expired date of the food product. This study aims to determine shelf life of natural food enhancer based on straw mushroom (Volvariella volvacea) using Accelerated Shelf Life Testing (ASLT). Determination of shelf life was carried out at 30, 40, 50°C followed by descriptive sensory (colour, aroma, taste) and moisture content analysis for 28 days. Further data of those parameter over storage time were plotted into a graph to obtain a linear regression equation of zero-order and first-order. Then relationship between ln slope (k) and storage temperature 1/T (°K) was plotted in a linear regression to determine activation energy. Equation of linear regression from the lowest activation value then was used to calculate the shelf life of the product. The taste parameter on zero-order was used in shelf life determination as the critical quality because it has the smallest activation energy as 1708.39 cal/mol. The Arrhenius equation then was calculated using this value to obtain degradation constant (k). The results showed that shelf life of the straw mushroom based food enhancer were 77.58 days at 30°C, 70.85 days at 40°C and 65.07 days at 50°C.

SHELF LIFE OF NORI FROM Gracilaria sp. WITH ALUMUNIUM FOIL PACKAGING BASED ON THE ACCELERATED SHELF LIFE TEST METHOD

This research was conducted at the Laboratory of Fisheries Products Processing, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Jatinangor. The purpose of this research is to determine the shelf life of Nori from Gracilaria sp. with the Accelerated Shelf Life Test (ASLT) method Arrhenius Model using Aluminum Foil packaging. Aluminum foil packaging is a package composed of hermetic, flexible, and opaque metal so that it has high protection properties against water vapor, light, grease and gas. The determination of the shelf life of the ASLT method is carried out using parameters of environmental conditions that can accelerate the process of product quality degradation, namely by storing the product at several temperatures above normal storage temperature. The observations used in determining the shelf life of Gracilaria sp. using a sensory test and a water content test with storage for 35 days, at a temperature of 25oC and 35oC. The result of the Arrhenius model calculation, the appearance parameter is selected as the critical parameter because it has the lowest Activation Energy (Ea) in determining the shelf life of Gracilaria sp. which is packaged using PE plastic. The results show that the parameters used to determine the shelf life of the product are the taste parameters based on the order 1 reaction with the Arrhenius Plot Ln K = 0.026 – 1318.4 (1 / T) and Ea of 2619.66 Kj/mol. The shelf life of Nori Gracilaria sp. if stored at 25oC temperature is 89 hari 7 jam.

The development of a functional adaptogenic beverage, using plant extracts of Centella asiatica and Hoodia gordonii

A functional beverage of adaptogenic action based on plant raw materials was developed using dry plant extracts of Centella asiatica and Hoodia gordonii to impart functional and biologically active properties. An organoleptic analysis, namely, the free profiling method, was used to compose the beverage recipe. Based on the constructed profilograms, the optimal ratio of the blend of juices and teas, which is 1: 0.65, was chosen. In beverage, the basic physicochemical parameters of the finished functional drink are determined, such as: solids content, total (titratable) acidity, hydrogen index (pH) of coloring substances (anthocyanins), macronutrients (Na, K, Mg, Ca) and trace elements (Fe, Cu, Zn, Mn). In accordance with the requirements of the technical regulation of Customs Union 021 “On Food Safety”, the adaptogenic beverage was tested for the content of toxic elements: arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg). As a result, the content of toxic elements doesn’t exceed the maximum permissible concentration. In the scientific work, we determined the shelf life of a functional beverage of adaptogenic and anti-alcohol action. Using the accelerated shelf life testing method (ASLT), which is based on the Arrhenius model, we determined the shelf life of the beverage = 3.7 months.

Shelf Life of Nori from Eucheuma cottonii with Aluminum Foil Packaging Based on the Accelerated Shelf Life Test Method

This research aims to determine the shelf life of Nori from Eucheuma cottonii in aluminum foil packaging using the Accelerated Shelf Life Test (ASLT) method with the Arrhenius approach model. This research was conducted using aluminum foil packaging to package Eucheuma cottonii nori products in 2 different storage temperatures, namely 25°C and 35°C. Observations were made on the 35th day with a countdown method which included sensory testing and moisture content testing. The data from the observations had analyzed the estimation of shelf life using the Arrhenius approach model. The results showed that the taste quality parameter is a critical parameter that determines the shelf life of Eucheuma cottonii nori using aluminum foil packaging. The shelf life of nori from Eucheuma cottonii using aluminum foil packaging has a shelf life of 2 months 14 days 17 hours.

Shelf Life of Nori from Gracilaria sp. with Polypropylene (PP) Packaging Based on the Accelerated Shelf Life Test Method

This research was conducted at the Laboratory of Fisheries Products Processing, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Jatinangor, West Java, Indonesia. The purpose of this research was to determine the shelf life of Nori from Gracilaria sp. with the Accelerated Shelf Life Test (ASLT) method Arrhenius Model using Polypropylene (PP) packaging. Gracilaria is a genus of red algae (Rhodophyta) used as a food for humans and various species of shellfish. Polypropylene (PP) is a shiny, clear plastic with good optical properties and tensile resistance. The determination of the shelf life of the ASLT method is carried out using parameters of environmental conditions that can accelerate the process of product quality degradation, namely by storing the product at several temperatures above normal storage temperature. The observations used in determining the shelf life of Gracilaria sp. using a sensory test and a water content test with storage for 35 days, at a temperature of 25°C and 35°C. The calculation results of the Arrhenius model used texture parameters as critical parameters because those had the lowest Activation Energy (Ea) in determining the shelf life of Gracilaria sp. which is packaged using PP plastic. The results showed the value used for determining the shelf life of nori Gracilaria sp. namely texture parameters based on the order 0 reaction with the Arrhenius plot Ln K = -2.0381 - 214.63 (1 / T) and Ea of 1784.6485. The shelf life of nori Gracilaria sp. was found to be elongated if stored at room temperature of (25°C) which was around 94 days.

The performance of beeswax coating containing vegetable oil-based lecithin as an emulsifier on weight loss and shelf life estimation of mango

Fruits are one of the agricultural commodities grown in Indonesia and mango constitutes the third largest. One of the disadvantages in transporting mangoes to long distant markets is the short shelf life when fruits are stored at the room temperature. Some efforts to maintain quality and freshness have been coating the fruits with natural wax/beeswax. This study aimed to determine the effects of beeswax coating containing vegetable oil-based lecithin on physical and weight loss in mango, as well as estimate the shelf life using the Accelerated Shelf-Life Testing (ASLT) method. The coating formulation consisted of beeswax, triethanolamine, stearic acid, vegetable lecithin (soybean: rice bran), and water. Mango coated with beeswax coating with a concentration of vegetable lecithin emulsifier were varied at 0.25, 0.5, 0.75, 1%, and control (without beeswax coating) stored at room temperature 301oK and cooler temperature 286oK. Samples were monitored for five weeks and evaluated periodically for physical change and weight loss. Beeswax coated mango exhibited slower rate of ripening than the control stored in both room and cooler temperatures. The lowest total weight loss of mangoes at both temperatures for 5 weeks with beeswax coating at 0.25% vegetable lecithin emulsifier was 7.25%. The estimation of shelf life using ASLT method and Arrheinus model to mango fruit coated with beeswax coating at 0.25% vegetable lecithin concentration as emulsifier was 36.91 days.

Shelf life study of Bolu Cukke using the Accelerated Shelf Life Testing (ASLT) method

The aim of this study was to determine the shelf life of Bolu Cukke in glazed paper packaging using the Accelerated Shelf Life Testing (ASLT) Method. Determination of shelf life using the ASLT method by knowing the critical moisture content and equilibrium content in Bolu Cukke. The results of critical moisture content and equilibrium content of Bolu Cukke indicate a number 2 indicating that it is at a critical point, shelf life is 6 days, with critical water content as much as 23.49% while the results using a 7-day labuza calculation showed no significant difference by storing it using critical water content. The results of the equilibrium value of 24.606% by producing a slope b value of 0.307 using the Henderson isothermic absorption curve with an MRD value of 3.1915. Estimation of shelf life of Bolu Cukke products using the labuza equation shows that the shelf life for Bolu Cukke products is 7 using glasin paper packaging which to protect the product from sunlight, air, and contamination of microorganisms. The change affected physically, chemically and microbiologically, which makes bolu cukke can no longer be consumed.

The Evaluation of shelf life of Arabica mixed coffee drinks using accelerated shelf life testing method.

Arabica coffee drinks in this study were made from three parts of coffee beans are cascara, which is the skin of coffee fruit; green coffee beans; and roasted coffee beans. This beverage product is then packaged and stored in a ready to drink glass bottle. This study aim to determine the shelf life of mixed arabica coffee beverage products, using the ASLT method. Samples were stored under different temperature conditions: 4oC, 27oC and 37oC. The test consists of measuring pH, total dissolved solids (ppm), total sugar (oBrix), total color difference (ΔE) and total plate count (log CFU/ ml). The results showed that during storage occurred to change the physicochemical properties and the number of microbes in the mixed arabica coffee drinks. Each parameter has a different activation energy value. The higher the storage temperature was proven to the smaller the activation energy, which indicates a shorter shelf life. This arabica coffee drink has a shelf life of 4 days if stored at 4oC.

Shelflife Estimation of Seaweed Dodol in OPP Packaging by Using Accelerated Shelf Life Test Method

AbstracThis research is carried out due to the limited information on the shelf-life of food product in Indonesia, especially in South Sulawesi. In addition, there is no sufficient information on the self-life of packed seaweed dodol which produced by using scientific method. Seaweed dodol has a high economic value. The objective of this research was to estimate the self-life of seaweed dodol which is produced in traditional scale (micro, small, and middle scale bussiness) using the scientific method. Accelerated Shelf Life Testing (ASLT) Method is one of methods used in determining the shelf life of seaweed dodol based on critical water content and graining as indicators of quality deterioration. The isotermic sorption model of products was obtained from isotermic sorption curve formed from the relationship between the values of water activity (aw) and the equilibrium moisture content (Me). Moreover, five different models of isotermic sorption were tested. They were Hasley, Chen-Clayton, Henderson, Caurie, and Oswin model. The results showed that the Henderson model was the appropriate model in describing the isotermic sorption phenomena at seaweed dodol with the Mean Relative Deviatian (MRD) of 1.4. By integrating the critical moisture content (Mc), packing permeability (OPP), sample weights (Ws), the surface area of packing (A) and the saturated vapor pressure (Po) into the equation Labuza, the self-life of seaweed dodol at temperature of 30 °C and RH of 78% was 56 days.

PENENTUAN UMUR SIMPAN CUKA KAKAO MENGGUNAKAN METODE ACCELERATED SHELF-LIFE TESTING (ASLT) DENGAN PENDEKATAN ARRHENIUS

Cocoa vinegar is a fermented vinegar produced from a product of cocoa bean fermentation. The aims of this study were to determine quality change of cocoa vinegar during retention and determine its self-life by using Accelerated Shelf-Life Testing (ASLT) method by Arrhenius Equation Approach. This research was conducted by using one factor, and it was storage temperature treatment that consisting of 3 levels, 30, 40 and 50?C. Retention and observation start from 0 week until 16 weeks, with parameters that observe were acetic acid, pH, total suspended solid and turbidity. Cocoa vinegar has decreased quality during storage, at 30?C, 40?C and 50?C. During storage of the three temperatures, the temperature of 50?C suffered the most rapid deterioration with the characteristic of decreasing the acetic acid content by more than 50%, and the increase of pH, total suspended solid and turbidity on the 63rd day. Shelf life of cocoa vinegar by using the characteristic of acetic acid with linear regression equation y = -4624.x + 10.30 with R² = 0.999.at storage from temperature 10, 20, 30, 40, and 50?C respectively were 431 days (14.3 months). 246 days (8.20 months). 146 days (4.86 months). 89 days (2.96 months).and 56 days (1.86 months).
 Keywords: vinegar of cocoa, self-life prediction, ASLT method

Shelf life prediction of apple brownies using accelerated method

The aim of this research was to determine shelf life of apple brownies. Shelf life was determined with Accelerated Shelf Life Testing method and Arrhenius equation. Experiment was conducted at 25, 35, and 45°C for 30 days. Every five days, the sample was analysed for free fatty acid (FFA), water activity (Aw), and organoleptic acceptance (flavour, aroma, and texture). The shelf life of the apple brownies based on FFA were 110, 54, and 28 days at temperature of 25, 35, and 45°C, respectively.