Acalypha Indica Research Articles

Biosynthesis, characterization, and In-Vitro Anticancer effect of Plant-Mediated Silver Nanoparticles using Acalypha Indica Linn: In-Silico Approach

Biosynthesis, characterization, and In-Vitro Anticancer effect of Plant-Mediated Silver Nanoparticles using Acalypha Indica Linn: In-Silico Approach

In-silico and in-vitro studies to identify potential inhibitors of SARS-CoV-2 spike protein from Omani medicinal plants

In the quest for novel therapeutic agents against SARS-CoV-2, the proposed study explores the potential of traditional Omani medicinal plants, focusing on the efficacy of natural ligands against the virus's Spike protein. Among 437 identified medicinal plants across Oman, 47 species that are documented for their traditional use in treating respiratory infections, with 30 species' ligands available were chosen for analysis. Molecular docking was performed using Autodock Vina on these ligands, yielding 406 unique ligands post-duplication removal. The binding affinities of target-ligand complexes were precisely determined, ranking them by interaction strength. This process identified Corilagin, a phytochemical from the Acalypha indica plant (locally known as Aeyan Al Aqrada), as the most promising inhibitor. Subsequent analyses using GROMACS for molecular dynamics simulation confirmed its binding stability and interaction dynamics of the Corilagin-protein complex. The in-vitro studies further validated Corilagin's inhibitory effect on SARS-CoV-2, demonstrating a remarkable 92 % inhibition at 0.5 mM concentration. Dilution studies to ascertain the IC50 value revealed Corilagin's high potency at a micromolar level (IC50 = 2.15 ± 0.13 μM), underscoring its potential as a drug candidate for SARS-CoV-2 treatment. These findings highlight the significance of ethnomedicine and in-silico methodologies in drug discovery, offering promising directions for future antiviral research.

HPLC Profile of Bioactives and Antioxidant Potential in Acalypha indica Extract

Acalypha indica is a plant found in many parts of Africa and Asia. The plant is used for the treatment of many respiratory diseases, management of cancers, diabetes and other diseases. The leaves of Acalypha indica were harvested, washed and shade dried. They were later extracted with methanol. The methanolic extract of Acalypha indica was subjected to DPPH radical scavenging assay, total antioxidant and HPLC quantification of phenols and flavonoids. The result showed a percentage yield of 5.72 %. Also the antioxidant capacity of Acalypha indicia based on the phosphomolybdenum method showed 13.60 ± 0.21 mg AAE/g extract. The DPPH radical scavenging potential of Acalypha indica showed that at 100 – 1000 µg/ml, the extract of Acalypha indica inhibited 7.2 ± 0.35 – 65.13 ± 1.53 % of DPPH radical as compared with standard gallic acid. The phenolics and flavonoids detected through HPLC are shown in Table 3. The phenolics and polyphenols with higher quantity in Extract of Acalypha indica are naringenin (169.91 mg/100 g), gallic acid (131.51 mg/100 g), rutin (76.72 mg/100 g), syringic acid (35.75 mg/100 g), ferulic acid (30.10 mg/100 g) and quercetin (11.36 mg/100 g). The results showed that Acalypha indica possesses antiradical, total antioxidant properties including a wide range of phenols and polyphenols. Therefore the extract can be a source of dietary supplement. This plant extract is a source of medicine if further research is applied.

Biogenic synthesis of porous CuO nanoparticles: Synergistic effects in photocatalytic dye degradation and electrochemical energy storage applications

This study explores the biogenic synthesis of CuO nanoparticles functionalized with Solanum nigrum, using Acalypha indica leaf extract as a reducing agent. The biogenically synthesized CuO nanoparticles were comprehensively characterized by various analytical techniques, involves X-ray diffraction analysis (XRD), BET surface area analysis (BET), energy-dispersive X-ray spectroscopy (EDAX), X-ray photoelectron spectroscopy (XPS), UV–Visible spectral analysis (UV), Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, photocatalytic degradation, antimicrobial assay, cytotoxicity assay, electrochemical analysis, and linear sweep voltammetry studies. The XRD analysis revealed end centered monoclinic phase with a 10 nm average crystalline size. BET analysis showed a mesoporous structure with a surface area of 11.54 m2/g. XPS and EDAX confirmed the presence of Cu2+ and O2− ions. SEM and TEM images indicated spherical, and porous nanostructures. UV–Visible spectroscopy identified an active optical range of 508–518 nm (2.40–2.45 eV), suggesting potential photocatalytic activity. The photocatalytic degradation efficiencies of Methylene blue (98.8 %) and Rhodamine B (95 %) were achieved after 100 min of irradiation. Antimicrobial tests demonstrated effectiveness against Staph. aureus, E. coli, Shigella flexneri, and Candida tropicalis. The nanoparticles exhibited notable cytotoxicity against colorectal cancer cells with IC50 values of 58.66 μg/ml and 66.78 μg/ml. Additionally, a symmetric supercapacitor electrode using these nanoparticles achieved a specific capacitance of 375 Fg−1 at the current density of 1 Ag−1. This research underscores the versatile applications of biogenic CuO nanoparticles in photocatalysis, antimicrobial treatments, cancer therapy, and energy storage.

Formulation And Evaluation of Herbal Tablets Containing Ethanolic Extract of Acalypha indica –A Quality By Design (Qbd) Approach

Formulation And Evaluation of Herbal Tablets Containing Ethanolic Extract of Acalypha indica –A Quality By Design (Qbd) Approach

LC[sbnd]HRMS-based metabolomics on Acalypha indica with different altitude and antioxidant activity

Achalypha indica is a weed that has medicinal properties. This study aims to determine the metabolite profile and evaluate the antioxidant activity of A. indica from areas with different altitudes, Tuban (20 to 50 masl; TBN), Banyuwangi (60 to 90 masl; BB), Magetan (300 to 600 masl; MGT), and Malang (500 to 800 masl; MLG). The ethanol extract of A. indica was analyzed for total phenolics and antioxidant activity. Separation and identification of samples were carried out using liquid chromatography-high-resolution mass spectrometry (LCHRMS), then classified by Principal Component Analysis (PCA). The total phenol value ranged from 6.83 to 10.67 mg GAE /g of dry powder, with an antioxidant IC50 of 86.09 to 118.96 µg/mL. LCHRMS successfully identified 83 metabolites. PCA analysis was able to clearly distinguish each sample based on the altitude of the growing location with a total variance value reaching 82 % (PC-1: 57 %, and PC-2: 25 %). Heat map analysis that observes variations in metabolite composition in each sample shows an increase in the content of flavonoid compounds in the lowlands under certain geographical conditions. Therefore, the height of the growing location has a significant influence on the metabolite profile and antioxidant activity. These findings are very important for assessing the quality, safety, and efficacy of medicinal plant raw materials.

Urine Glucose Detection Via Gold Nanoparticle Formation Using 3D-Connector Microfluidic Paper Based Analytical Devices

A metabolic disorders that have experienced a significant increase in the world are diabetes mellitus. Diabetes is caused by two main factors: the first is damage to pancreatic beta cells, which prevents insulin from being produced, and the second is impaired insulin secretion and function. Chronic diabetes, if not treated properly, can lead to acute complications including eye, kidney, lung, nerve, and even death. Diabetes can be diagnosed through blood and urine. In general, glucose detection is carried out using invasive methods that use blood samples, which can cause pain and discomfort for users. Current research is developing non-invasive glucose detection using urine samples. This research aims to develop non-invasive glucose detection technology using 3D-connector μPADs (Microfluidic Paper Based Analytical Devices) which have the advantages of being safe, easy, and simple. The three-dimensional connector on the device functions as a connector to facilitate the coordination of fluid flow in the sample zone and detection zone. The glucose detection method uses gold (III) chloride as a gold nanoparticle (AuNPs) precursor, an aqueous extract of Acalypha indica Linn as a stabilizing agent, sodium hydroxide (NaOH) as a catalyst, and glucose in artificial urine as a sample. Method validation results using imageJ software indicated linearity with a coefficient of determination value (R2) of 0.9714, precision with a %RSD value (Relative Standard Deviation) of 2.69, and an accuracy level ranging from 92.22-99.23%.

Documentation On Medicinal Plants of Khanapur Forest Bidar District, Karnataka

The present study was undertaken to document medicinal plants of Khanapur forest Bidar district, karnataka the documentation of medicinal plants helps to recognize the overall ecological conditions and gives profound understanding of the economic medicinal and traditional importance of medicinal plants diversity. The present research documentation work was started in November 2023 and completed in February 2024. The present study reported that 119 Medicinal plant species belongs to 54 families distributed in Khanapur forest Bidar included angiosperms Pteridophytes and Gymnosperms. Abrus precatorius, Ephedra, Withania sominifera, Catharanthus roseus, Tinospora cardifolia, Andrographis paniculata, Phyllanthus amarus, allium sativum, Cocos nucifera, Coleus aromaticus, Curcuma longa, Martynia annua, Ocimum tenuiflorum, Tamarindus indica, Terminalia chebula,Tithonia diversifolia, Acalypha indica, Brassica juncea, Clitoria ternatea, Datura metal, Delonix elata, Euphorbia antiquorum, Luffa acutangula, Madhuca longifolia, Myristica fragrans, Santalum album and Vitex negundo are some of the important medicinal plants of Khanapur forest Bidar district of karnataka.

Documentation On Medicinal Plants of Deva Deva Vana Botanical Garden of Bidar District, Karnataka

The present study was undertaken to document medicinal plants of Deva Deva vana Botanical garden of Bidar district, karnataka. Deva Deva vana literally means ‘Forest of divine/celestial beings’ A Botanical garden maintained by the Forest department around 6 km from Bidar – Hyderabad Highway. It is home to around 200 medicinal plants and trees, and considered sacred. It is divided into around 10 gardens and a nursery which is classified according to a particular theme like Nandana vana, Ashoka vana, Navagraha vana, Rock Garden, shiva panchayath vana, Phala pushpa vana,..etc the documentation of medicinal plants helps to recognize the overall ecological conditions, conservation and gives profound understanding of the economic medicinal and traditional importance of medicinal plants diversity. The present research documentation work was started in February 2023 and completed in June 2024. The present study reported that 147 Medicinal plant species belongs to 64 families distributed and conserved in Deva Deva vana Botanical garden Bidar included angiosperms Pteridophytes and Gymnosperms. Abrus precatorius, Ephedra, Withania sominifera, Catharanthus roseus, Tinospora cardifolia, Andrographis paniculata, Phyllanthus amarus, allium sativum, Cocos nucifera, Coleus aromaticus, Curcuma longa, Martynia annua, Ocimum tenuiflorum, Tamarindus indica, Terminalia chebula,Tithonia diversifolia, Acalypha indica, Brassica juncea, Clitoria ternatea, Datura metal, Delonix elata, Euphorbia antiquorum, Luffa acutangula, Madhuca longifolia, Myristica fragrans, Santalum album and Vitex negundo are some of the important medicinal plants of Deva Deva vana Botanical garden Bidar district of karnataka.

Green Synthesis of Tannic Acid-Mediated Chromium Oxide Nanocomposites using Acalypha Indica and Carica Papaya Leaf and its Biomedical Applications

Green Synthesis of Tannic Acid-Mediated Chromium Oxide Nanocomposites using Acalypha Indica and Carica Papaya Leaf and its Biomedical Applications

Antioxidant activities of ethanolic extract of Acalypha indica Linn

Antioxidant activities of ethanolic extract of Acalypha indica Linn

QUALITATIVE PHYTOCHEMICAL ANALYSIS OF ACALYPHA INDICA L. MEDICINAL PLANTS OF FAMILY EUPHORBIACEAE

A preliminary analysis was conducted on the aqueous extract of the leaves, stem, and root of Acalypha indica L. to determine its phytochemical composition. This plant is classified under the family Euphorbiaceae. This plant is well recognized in traditional medicine for its efficacy in treating a variety of diseases. The analysis was conducted using conventional methodologies. The medical importance of many phytochemical elements revealed in this plant confirms the long-standing practice of using medicinal plants as treatments for human ailments. The objective of this research was to identify the main phytoconstituents present in Acalypha indica L. medicinal plants and establish a correlation between their presence and the bioactivities shown by the plants. The plant was screened using conventional techniques, which revealed the presence of tannins, flavonoids, phenolics, saponins, steroids, cardiac glycosides, and alkaloids. The existence of these phytochemicals may be associated with the therapeutic capacity of certain plants. Additional research is required to assess the pharmacological capabilities of this plant, as well as to separate, analyse, and understand the chemical structures of the bioactive chemicals that are responsible for its activities and other therapeutic properties.

Ecotoxicological Effect on Modulation of Bioactive Molecules in the Medicinal Plant Acalypha indica

Ecotoxicological Effect on Modulation of Bioactive Molecules in the Medicinal Plant Acalypha indica

Synergistic remediation of hydrocarbons using a hybrid approach: Harnessing biosurfactant and silver nanoparticles

A biosurfactant is an amphiphilic molecule that has both hydrophobic and hydrophilic properties. Biosurfactants from microbial sources with oil degradation potential were isolated from hydrocarbon-contaminated sites. Oil displacement and emulsification tests in this study identified the biosurfactant produced. The biosurfactants produced by the isolated bacteria possess anti-microbial properties. The cell-free supernatants of Bacillus subtilis and Pseudomonas aeruginosa showed positive results for the oil displacement method. The drop collapse test supported the biosurfactant production by these isolates. The synthesis of silver nanoparticles from Acalypha indica plant leaf extract was carried out. The biosurfactants (BS) and silver nanoparticles (AgNP) was also carried out. Their application as individual and synergistic effects in the bioremediation of hydrocarbons (engine waste oil) was discussed. Gravimetric analysis was used to analyse the degradation of engine oil. Silver nanoparticles showed absorbance in the range of 200 and 400 nm. Seven discrete peaks were obtained in FT-IR, representing the main functional group of AgNPs. The reduction of silver nitrate (AgNO3) into silver nanoparticles (AgNP) occurred due to the presence of biomolecules. The SEM analysis showed that the size of the nanoparticle is between 69 and 87 nm and is crystalline. The average distribution of the diameter of the silver nanoparticles was 186.9 nm. Broader peaks of XRD reflect the influence of the experimental circumstances on the nucleation and development of the crystal nuclei and indicate smaller particle sizes. The biosurfactants showed less degradation than the synergistic method employed with biosurfactants and silver nanoparticles. Therefore, it is concluded that the silver nanoparticles influenced the biosurfactant and increased the rate of degradation of the engine waste oil.

Phytochemical Screening, Acute Toxicity and Evaluation of in vitro Antiurolithaitic Activity of Ethanolic and Methanolic Root and Leaf Extracts of Acalypha indica Linn.

Phytochemical Screening, Acute Toxicity and Evaluation of in vitro Antiurolithaitic Activity of Ethanolic and Methanolic Root and Leaf Extracts of Acalypha indica Linn.

Combatting microbial biofouling network associated with Chembarambakkam Lake water using Acalypha indica derived metabolites

AbstractThis study assessed the anti‐biofouling potential of Acalypha indica ethanolic extract against biofouling communities associated with Chembarambakkam Lake water under in vitro conditions. Water samples from the lake were collected at various seasons, and changes in various physicochemical parameters and microbial status were evaluated. Most significantly, the efficacy of the microflora associated with water‐forming biofouling or biofilm network on its susceptibility to the ethanolic extract of A. indica was investigated. Distinct changes were recorded in all the tested physico‐chemical and microbiological statuses in all the seasons. The efficacy of biofouling or biofilm network formation was found to be statistically high during the winter season. Biofouling or biofilm formation was susceptible to the ethanolic extract of A. indica. A. indica treatment brought about notable changes in the morphology of the biofouling networks. A. indica bioactive compounds such as 9,10‐dideutero octadecanal and cyclononasiloxane, octadecamethyl known for their antimicrobial and in vitro properties, effectively prevented biofilm and biofouling formation.

Weed Biology and Ecology Studies: Diversity, Dominance and Prediction of Yield Loss of Corn (Zea mays L.) Due to Broadleaf Weeds Competition in Dryland

The aim of the research was to determine the diversity, distribution, dominance, and yield loss of corn due to competition from broadleaf weeds. Descriptive research method with direct survey techniques of research objects. Data were collected using 10 sample plots measuring 1 m2, which were placed using random sampling. Observation parameters include the population and dry biomass weight of weeds and corn. The data was analyzed descriptively and comparatively by calculating density, frequency, and dominance to obtain an important value index and summation diminance ratio (SDR). The results of the research showed that 14 species of broad-leaved weeds were found with high species diversity, distribution, dominance, and abundance indices. The competitiveness and relative weighted dominance of six weed species, namely Amaranthus spinusus, Amaranthus gracilis, Synedrella nodiliflora, Acalypha indica, Ageratum conyzoides, and Alternanthera philoxeroides, are higher than those of other weed species, causing corn yield losses of 5.56%–21.90%. Therefore, these six weed species must be controlled.

Effect Of Anting-Anting Leaf Extract (Acalypha Indica L.) On Lowering Blood Glucose Rats (Rattus Norvegicus) Induced By Sukrosa

Anting-anting leaves (Acalypha indica L.) are weeds that have various properties, one of which is as a lowering blood glucose levels. The chemical content contained in the form of flavonoids that function as inhibitors of α-glucosidase, maltase, α-amylase enzymes, tannin substances are also known to function as astrigents that can inhibit glucose intake is not excessive. The purpose of this study is to determine whether there is an effect of anting-anting leaf extract (Acalypha indica L.) on reducing blood glucose levels in rats (Rattus norvegicus) induced by sucrose. This research used 20 rats (Rattus norvegicus) as test animals which were divided into 5 groups. The group consisted of negative control group, positive control and 3 dose groups. The doses used were 500 mg/kgBB, 1000 mg/kgBB and 1200 mg/kgBB. Mice were made hyperglycemia with sucrose induction which was then given anting-anting leaf extract (Acalypha indica L.) according to lower glucose levels in mice. The results obtained were continued with the two-way ANOVA test and obtained significant results indicated by p < 0.05 (0.005) which showed there was a significant effect of anting-anting leaf extract (Acalypha indica L.) on reducing blood glucose levels in sucrose-induced rats (Rattus norvegicus). Significant results were continued with the post hoc test and obtained significant results.

Five records of new and rare alien species to the flora of the United Arab Emirates (UAE)

The article presents new records for five alien species previously unknown from the flora of the United Arab Emirates (UAE) or very rare in the country – Chenopodium ficifolium (Chenopodiaceae/Amaranthaceae s. l.), Acalypha indica, Euphorbia maculata (Euphorbiaceae), Gamochaeta pensylvanica (Gnaphalium pensylvanicum), and Verbesina encelioides (Asteraceae). Some of them have been recorded for the first time from the emirate Fujairah in north-eastern part of the UAE. Euphorbia maculata, which is rare in the country, was found only in “Salman Nursery” at Masafi town and in an irrigated garden near village Bithna, Chenopodium ficifolium was found in environs of Al Siji, in Wadi Siji on the road-dam and in “Al Phoenician Nursery” at Al Dibba town, Acalypha indica L. grows as weed in a plant market on the roadside in Al Bidya and in “The Green Nursery Sales Dibba” at Al Dibba town, they are new for the flora of Fujairah and UAE at all. Gamochaeta pensylvanica was found in a small quantity in a plant market and plant nursery in the Masafi friday market, and Verbesina encelioides – was found naturalized in Khor-Fakkan (Emirate of Sharjah) – both are very rare in the UAE. Species, synonyms, spatial distribution, habitat preferences, and species taxonomy with remarks on identification and differentiation from the most similar taxa occurring in the study area, as well as the list of localities are presented. The herbarium materials were transferred to the Herbarium of the Komarov Botanical Institute (LE, St. Petersburg, Russia), the duplicates – to the Herbarium of Altai State University (ALTB, Barnaul, Russia) and the Scientific Herbarium of Fujairah (FSH, Wadi Wurayah national park, Fujairah, United Arab Emirates).

First Report of Powdery Mildew Caused by Podosphaera xanthii on Acalypha indica in China.

Acalypha indica L. is an annual erect herb of the Euphorbiaceae family. This plant is found widely in the tropics and parts of Africa and Asia (Chakraborty et al. 2023). In China, A. indica is a vegetable and also used as a folk medicine due to its antipyretic and hemostatic, antibacterial and anti-inflammatory properties. In February 2022 and 2023, powdery mildew symptoms were observed on 70% of A. indica plants on the Hainan Medical University campus (19° 58' 53″ N; 110° 19' 47″ E) in Haikou, Hainan Province, China. Powdery mildew colonies covered the leaf surfaces and stems of affected plants, causing discoloration and defoliation. Mycelia were superficial and hyphal appressoria were nipple-shaped. Conidiophores (n =30) were unbranched, cylindrical, 66 to 150 × 10 to 15 µm, and produced three to five immature conidia in chains with a crenate outline. Foot cells (n =30) were cylindrical, straight or sometimes curved at the base, and 31 to 59 µm long. Conidia (n =100) were ellipsoid-ovoid to doliiform, 20 to 33 ×12 to 20 µm (length/width ratio = 1.3 to 2.4), with well-developed fibrosin bodies, and produced germ tubes from the lateral position. Based on these morphological characteristics, the pathogen was provisionally identified as Podosphaera xanthii (Braun and Cook 2012). The teleomorph was not observed. A specimen was deposited in the Hainan Medical University Plant Pathology Herbarium as HMAI-23. To confirm the genus identification and ascertain a putative species, genomic DNA was extracted from mycelium, conidiophores, and conidia using a fungal DNA kit (Omega Bio-Tek, USA). The rDNA internal transcribed spacer (ITS) region was amplified with primers ITS1/ITS4 (White et al. 1990) and sequenced directly. The resulting 575-bp sequence was deposited in GenBank (accession no. OR775733). A BLASTn search in GenBank of this sequence showed 99% similarity with the ITS sequences of P. xanthii on plants of Fabaceae, Malvaceae and Cucurbitaceae family from China (MH143485, MT242593, MK439611 and MH143483), Thailand (LC270779 and LC270778), Korea (MG754404), Vietnam (KM260704), and Puerto Rico (OP882310). Additionally, the 28S rDNA region was amplified using the primer pairs NL1 and NL4 (O´Donnell 1993; accession no. OR784547). This region shared 99% similarity with P. xanthii isolates (LC371333, LC270780, AB936277, and OP765401) as well. To confirm pathogenicity, five healthy potted plants of A. indica were inoculated by gently pressing a powdery mildew-infected leaf onto 15 young leaves. Five non-inoculated plants served as controls. All plants were maintained in a greenhouse at 24 to 30°C, 70% relative humidity, with a 16-h photoperiod. After 7 days, inoculated leaves showed powdery mildew symptoms whereas no symptoms were observed on control plants. The fungal colonies observed on inoculated plants were morphologically identical to those found on the originally infected leaves collected from Hainan Province. Based on the morphological characteristics and molecular identification, the fungus was identified as P. xanthii. In different countries and regions, P. xanthii has been previously reported on A. indica from Sudan and India (Amano 1986). To our knowledge, this is the first record of P. xanthii infecting A. indica in China. We are concerned that the pathogen could become a threat to the widespread planting of A. indica in the future.