This study assessed the role of mirrors of knowledge in the management and preservation of indigenous knowledge for sustainability and development in Ghana. The qualitative research design and structured interview were used in this study. The study focused on three categories of mirrors of knowledge: libraries (academic libraries, research libraries, special libraries, and public and community libraries), museums, and archive centers. Thematic content analysis was used to analyze the data collected via the interview. The study established that the managers and officers of the mirrors of knowledge understand and appreciate the concept of indigenous knowledge; there was the availability of technological systems and tools to facilitate the management and preservation of indigenous knowledge; and the mirrors of knowledge played a significant role in the management and preservation of knowledge. However, the absence of a collection development policy on indigenous knowledge; the outdated nature of most of the technological tools; the lack of funding; the lack of appreciation of indigenous knowledge by the staff and community members; etc. affected the quest to effectively manage and preserve indigenous knowledge for sustainable development. This study will serve as a guide for managers and policymakers of all the categories of mirrors of knowledge on the need, role, and importance of a collection development policy for indigenous knowledge, and guide in the identification and deployment of the appropriate technological systems for the management and preservation of indigenous knowledge. It will also inform the managers, policymakers, and stakeholders of indigenous knowledge of the challenges associated with the collection and management of indigenous knowledge and measures that can be deployed to help solve these problems for sustainability and development.