Plant Acacia Research Articles

Toxic effects of purified phenolic compounds from Acacia nilotica against common cutworm

Phenolics are the most abundant secondary metabolites of plants that are widely distributed in the plant kingdom. In the last few years, the development and identification of phenolic compounds from various plants have become a major area of the environment and health-related research. In the present study, different phenolic compounds were purified from the bark of the medicinally important plant Acacia nilotica which is rich in polyphenols and were evaluated for their insecticidal potential against a polyphagous pest, Spodoptera litura (Fab.). The compounds viz. Catechin, Chlorogenic acid, and Umbelliferone were purified from ethyl acetate-acetone (E-AE) fraction using Semi-preparative HPLC and were identified using melting point determination, Nuclear Magnetic Resonance (NMR), and Mass Spectroscopy (MS). These phenolic compounds recorded detrimental effects on the growth and development of second instar larvae (6 days old) of S. litura. The larval growth, survival, adult emergence, pupal weight, and different nutritional indices were adversely affected by the various concentrations of these purified compounds. The findings revealed the insecticidal potential of polyphenols obtained from the bark of A. nilotica, which can provide an alternative for resistance management, as these plant phytochemicals are highly effective against insecticide-resistant insect pests.

Phytochemical screening of Acacia auriculiformis leaf ethanol extract

Indonesia is one of the countries that has a very diverse biodiversity of more than 38,000 plant species, about 55% of which are endemic plants. Based on data from the WHO (World Health Organization), approximately 3.4 billion people in developing countries depend on traditional medicine derived from plants. In fact, about 20,000 plant species originating from the Indonesian Tropical Forests are widely used as ingredients for natural medicines by residents throughout Indonesia, one of which is the acacia plant. (Data from the Ministry of Environment in 2010 The purpose of this study was to determine the content of secondary metabolites contained in the ethanol extract of acacia leaves. phytochemical tests that have been carried out using ethanol solvent, obtained secondary metabolite content in the ethanolic extract of acacia leaves, namely alkaloids, flavonoids, phenols and saponins while terpenoid compounds were not found. This is because the solvent used is ethanol which is polar. Alkaloid compounds, flavonoids, and saponins are polar and generally can be attracted by polar solvents such as ethanol. Terpenoid compounds were not found because these compounds are non-polar and can be dissolved by non-polar solvents such as n-hexane.

Isolation, Docking and In Silico ADME-T Studies of Acacianol: Novel Antibacterial Isoflavone Analogue Isolated from Acacia leucophloea Bark

The plant Acacia leucophloea (Roxb.) Willd. of the family Fabaceae is of paramount importance in Indian medicine. We sought to evaluate the in vitro anti-microbial activity of A. leucophloea stem bark extract together with its phytochemical characterization and exploration of drug-likeness attributes. In vitro Kirby-Bauer disc-diffusion and tube-dilution assays were exploited for determining the anti-microbial activity of the methanolic bark extracts against several bacterial test strains. Spectral characterization of the isolated phytoconstituents was performed using spectroscopy techniques viz., UV, IR, 1H NMR, and mass spectroscopy followed by the in silico studies like docking and ADME-T studies. The crude methanolic extracts were active against all the bacterial test strains, albeit weakly or moderately, as indicated by the zone of inhibition and minimum inhibitory concentration in the anti-microbial assays. The isolated phytoconstituent was identified to be 3-(3,4-dihydro-5-methoxy-2H-chromen-6-yl)-2,5- dimethoxy-2H-chromen-7-ol (hereby coined as acacianol), a novel isoflavone analog. Acacianol demonstrated a strong binding affinity towards the bacterial DNA gyrase over clorobiocin, especially in the case of cavity 4 with no predicted toxicities in silico, except skin sensitization and chromosome damage.

Selecting Plant Types to Control Air Pollution and Developing Software to Plan Green Open Space in the Urban Area

The decrease in air quality becomes one of the development impacts that must be controlled deliberately. Planting trees is crucial for reducing the total number of dust and gas pollutants as it can restrain air pollution through absorption and adsorption. This research aimed at (a) determining the most appropriate types of plants for controlling air pollution and (b) developing software to plan the need for Green Open Space (GOS) in the urban city. The criteria of assessment for measuring the ability of a plant to absorb pollutant gas consisted of: (a) the density level of tree-crown, (b) plant combination, (c) thickness level of leaf, (d) the total number of leaves, and (e) plant distance. Meanwhile, the criteria for assessing the ability of the plant to adsorb dust particles comprised: (a) the roughness of leaf surface structure, (b) leaf width, (c) density level of tree-crown, (d) texture of stem skin surface, and (e) density level of the twig. The results of the research demonstrated that plants of Angsana (Pterocarpus indicus), Kihujan (Samanea saman), and Acacia (Acacia auriculiformis) were very appropriate for absorbing pollutant gas. Meanwhile, the good plants for adsorbing dust particles involved Angsana, Kihujan, Acacia, Tanjung (Mimusops elengi), Kersen (Mutingia calabura), Ketapang (Terminalia cattapa), and red Dadap (Erythrina crista-galli) by the level of adsorption ability around 65-75%. The successfully developed software could present suitability between Green Open Space (GOS) calculated manually and the one counted by software.

Phytochemical composition of different plant parts of Acacia nilotica (L.) and their medicinal values

Plant-derived medicines are long being used for the prevention and treatment of various human ailments. For the last few decades, plants are widely being explored for their active ingredients due to their immense potential in the treatment of critical illnesses. Thus, in recent years, exponential growth can be seen in the field of herbal medicines. Medicinal plants are a unique source of valuable phytochemicals. Their use in different medicine systems is gradually increasing due to their cost-effectiveness, easy availability and natural origin with fewer or no side effects. Acacia nilotica (L.) is a member of the family Fabaceae, commonly found in tropical and sub-tropical regions and the plant is widely known for its enormous medicinal values. Every plant part of A. nilotica is a source of many bioactive important secondary metabolites that are widely useful for the cure of various human diseases and the development of new drugs. An exhaustive literature survey revealed that tannins, flavonoids, alkaloids, polyphenols, fatty acids and carbohydrates are present as major classes of phytochemicals in different plant parts of A. nilotica. These phytochemicals exhibit significant antioxidant, anti-inflammatory, antibacterial, antifungal, antidiarrheal, antihypertensive, antispasmodic, anthelmintic, antiplatelet aggregation, anticancer and antiviral activities. The present review is aimed to organize the comprehensive information available on phytochemical composition and medicinal properties of different plant parts of A. nilotica viz. leaves, bark, flowers, seeds, pods, gum and roots. The study is useful to explore the therapeutic potential of different plant parts of A. nilotica which will further help in the development of new promising, safe, cost-effective drugs with a high therapeutic index from the different parts of the Acacia plant.

Limited ant co-occurrence and defensive mutualism in Acacia plants in a West African savanna.

Our understanding of the role of fire and effect of ant species composition, beyond their diversity and abundance, on the effectiveness of mutualism defence is limited. Most of our knowledge of ant–plant defence in tropical Africa is biased towards East African savannas which have richer soil, higher primary productivity and a more diverse arthropods and mammal community than West African savannas. We assessed the diversity of ant species associated with Acacia species in the Pendjari Biosphere Reserve in the Dahomey Gap, and their impacts on elephant damage. Elephant damage, ant diversity and abundance were measured in stands of five Acacia species. Eleven ant species were identified in the Acacia stands. The composition of these ant communities varied across Acacia species. Pair of ant species co-occurred in only 2 % of sampled trees, suggesting a strong competitive exclusion. Within this annually burnt environment, ants were rare on small trees. The intensity of elephant-caused branch breaking did not vary between trees with ants and trees without ants, suggesting limited Acacia–ant mutualism. Such limited biotic defence may mask strong physical and chemical defence mechanisms of Acacia trees against elephant damage. Ant assemblages in West Africa, unlike those in the more productive East Africa, are particularly species-poor. However, there is a convergence between these two regions in low rate of ant co-occurrence which might indicate strong competitive exclusion. Our study suggests that such low ant species richness while limiting the efficacy of mutualism in controlling mega-herbivore damage may mask a strong defence syndrome.

Two New Antiprotozoal Diterpenes From the Roots of Acacia nilotica.

The powdered roots of the medicinal plant Acacia nilotica were extracted with hexane and ethyl acetate, and the extracts were subjected to column chromatography for the isolation of potentially bioactive compounds and their screening against kinetoplastid pathogens. NMR and HREI mass spectrometric analyses identified two new diterpenes, characterized as 16, 19-dihydroxycassa-12-en-15-one (Sandynone, 1) and (5S, 7R, 8R, 9R, 10S, 13Z, 17S)-7,8:7,17:16,17-triepoxy-7,8-seco-cassa-13-ene (niloticane B, 2). The previously reported (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-diene-7,17-diol (3), (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-dien-7-ol-17-al (4), and (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-dien-7-ol (5) a, mixture of stigmasterol (6a) and sitosterol (6b), and lupeol (7) were also isolated. Several column fractions displayed significant activity against a panel of Trypanosoma and Leishmania spp., and from the most active fraction, compound 4 was isolated with high purity. The compound displayed high activity, particularly against T. brucei, T. evansi, and L. mexicana (0.88–11.7 µM) but only a modest effect against human embryonic kidney cells and no cross-resistance with the commonly used melaminophenyl arsenical and diamidine classes of trypanocides. The effect of compound 4 against L. mexicana promastigotes was irreversible after a 5-h exposure, leading to the sterilization of the culture between 24 and 48 h.

Novel Fusarium mutualists of two Euwallacea species infesting Acacia crassicarpa in Indonesia

ABSTRACT Several species in the Euwallacea fornicatus complex have emerged as important pests of woody plants globally, particularly in habitats where they are invasive aliens. These beetles live in obligate symbioses with fungi in the genus Fusarium. In this study, we identified Euwallacea spp. and their fungal mutualists that have emerged as pests of planted Acacia crassicarpa in Riau, Indonesia. Morphological identification and phylogenetic analyses of the mitochondrial cytochrome oxidase c subunit I (COI) gene confirmed that E. similis and E. perbrevis are the most abundant beetles infesting these trees. Multilocus phylogenetic analyses of their fungal mutualists revealed their nonspecific association with six Fusarium species. These included F. rekanum and five novel Fusarium mutualists within the Fusarium solani species complex (FSSC), four of which reside in the Ambrosia Fusarium Clade (AFC). These new species are described here as F. akasia, F. awan, F. mekan, F. variasi, and F. warna.

KEANEKARAGAMAN JENIS TUMBUHAN BAWAH TANAMAN AKASIA (Acacia mangium Willd) DI BUKIT PENGHIJAUAN MANDOR KABUPATEN LANDAK

Acacia mangium plantations in Bukit Penghijauan has grown by various species of understoreys. This study aims are to examine uderstoreys diversity and determine the amount of species and dominant of understoreys that’s growing under Acacia stands in Bukit Penghijauan Mandor Landak District. The study used a survey method with a systematic sampling of squares. Laying of plots was carried out by purposive sampling in area where are acacia plants. The size of plot is 10 m x 10 m, devided in to 25 sample plots of size 2 m x 2 m. Prymary data on taken involve species name, amount of individuals each species and herbarium specimens as identification device. The study result swowed that understoreys that be discovered under acacia stands in Bukit Penghijauan Mandor Landak District as much 21 species. The domination that species is paku resam (Gleichenia linearis Burm Clarke), rumput bede (Paspalum conjugatum Berg) and akar rambat (Psychotria sarmentosoides).Keywords : Bukit penghijauan, Species diversity, Understoreys.

Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya.

The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical approaches, we decipher specific plant-feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya. Cold-anthrone test indicative of recent plant feeding showed that fructose positivity rates were similar in both sand fly sexes and between those sampled indoors and outdoors. Analysis of derived sequences of the ribulose-1,5-bisphosphate carboxylase large subunit gene (rbcL) from fructose-positive specimens implicated mainly Acacia plants in the family Fabaceae (73%) as those readily foraged on by both sexes of Phlebotomus and Sergentomyia. Chemical analysis by high performance liquid chromatography detected fructose as the most common sugar in sand flies and leaves of selected plant species in the Fabaceae family. Analysis of similarities (ANOSIM) of the headspace volatile profiles of selected Fabaceae plants identified benzyl alcohol, (Z)-linalool oxide, (E)-β-ocimene, p-cymene, p-cresol, and m-cresol, as discriminating compounds between the plant volatiles. These results indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor- bait technologies control strategies.

Hydrocyanic acid poisoning in goats due to ACACIA (Acacia leucophloea) plant and its therapeutic management

Hydrocyanic acid poisoning in goats due to ACACIA (Acacia leucophloea) plant and its therapeutic management

Investigation of the Potential Use, Phytochemical and Element Contents of Acacia Plant Seeds Grown in Wild Form, Considered as Environmental Waste.

In this study, the effect of altitude on oil amounts, antioxidant activity, polyphenol content and mineral contents of Acacia seeds collected from two different locations (up to 1100 m above sea level) was investigated. Total carotenoid and flavonoid contents of Acacia seeds were detected as 0.76 (Konya) and 1.06 µg/g (Taşucu-Mersin) to 1343.60 (Konya) and 184.53 mg/100 g (Taşucu-Mersin), respectively. Total phenol contents and antioxidant activity values of Acacia seeds were identified as 255.11 (Konya) and 190.00 mgGAE/Taşucu-Mersin) to 64.18% (Konya) and 75.21% (Taşucu-Mersin), respectively. The oils extracted from Acacia seeds in Konya and Mersin province contained 62.70% and 70.39% linoleic, 23.41% and 16.03% oleic, 6.45%and 6.04% palmitic and 2.93% and 4.94% stearic acids, respectively. While 3,4-dihydroxybenzoic acid amounts of seeds are determined as 3.89 (Konya) and 4.83 mg/100 g (Taşucu-Mersin), (+)-catechin contents of Acacia seeds were identified as 3.42 (Konya) and 9.51 mg/100 g (Taşucu-Mersin). Also, rutintrihydrate and ferulic contents of Acacia seeds were found as 23.37 (Konya) and 11.87 mg/100 g (Taşucu-Mersin) to 14.74 mg/100 g (Konya) and 1.12 mg/100 g (Taşucu-Mersin), respectively. Acacia seeds collected from Konya and Mersin contained 4003.75 and 3540.89 mg/kg P, 9819.12 and 16175.69 mg/kg K, 4347.47 and 5078.81 mg/kg P, 2195.77 and 2317.90 mg/kg Mg, 1015.75 and 2665.60 mg/kg S and 187.53 and 905.52 mg/kg Na, respectively.

A Synthetic Bioactive Peptide Derived from the Asian Medicinal Plant Acacia catechu Binds to Dengue Virus and Inhibits Cell Entry.

Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represent an attractive alternative for development into anti-DENV drugs due to their safety and their diverse biological and chemical properties. We recently reported novel bioactive peptides extracted from the Asian medicinal plant Acacia catechu that efficiently inhibited all four DENV serotypes. In this study, we investigated the anti-DENV activity of a synthetic bioactive peptide derived from this plant. The most effective peptide (designated Pep-RTYM) inhibited DENV infection with a half-maximal inhibition concentration value of 7.9 μM. Time-of-addition study demonstrated that Pep-RTYM interacted with DENV particles and inhibited cellular entry. Pep-RTYM at 50 μM significantly reduced DENV production in Vero-kidney epithelial cells about 1000-fold, but it could decrease the virus production in Huh7 hepatocyte cells approximately 40-fold. Binding of Pep-RTYM to DENV particles may prevent virus interaction with cellular receptor and subsequent virus entry. This finding suggests a potential role of Pep-RTYM in the development of a novel anti-DENV drug.

Plant Growth-Promoting Rhizobacteria Isolated from Degraded Habitat Enhance Drought Tolerance of Acacia (Acacia abyssinica Hochst. ex Benth.) Seedlings.

Drought stress (DS) is the most impacting global phenomenon affecting the ecological balance of a particular habitat. The search for potential plant growth-promoting rhizobacteria (PGPR) capable of enhancing plant tolerance to drought stress is needed. Thus, this study was initiated to evaluate the effect of inoculating Acacia abyssinica seedlings with PGPR isolated from rhizosphere soil of Ethiopia to enhance DS tolerance. The strains were selected based on in vitro assays associated with tolerance to drought and other beneficial traits such as salinity, acidity, temperature, heavy metal tolerances, biofilm formation, and exopolysaccharide (EPS) production. The strains with the best DS tolerance ability were selected for the greenhouse trials with acacia plants. The results indicate that out of 73 strains, 10 (14%) were completely tolerant to 40% polyethylene glycol. Moreover, 37% of the strains were strong biofilm producers, while 66 (90.41%) were EPS producers with a better production in the medium containing sucrose at 28 ± 2°C and pH 7 ± 0.2. Strains PS-16 and RS-79 showed tolerance to 11% NaCl. All the strains were able to grow in wider ranges of pH (4–10) and temperature (15–45°C) and had high tolerance to heavy metals. The inoculated bacterial strains significantly (p ≤ 0.05) increased root and shoot length and dry biomass of acacia plants. One of the strains identified as P. fluorescens strain FB-49 was outstanding in enhancing DS tolerance compared to the single inoculants and comparable to consortia. Stress-tolerant PGPR could be used to enhance acacia DS tolerance after testing other phytobeneficial traits.

ANALISIS POTENSI TANAMAN HUTAN INDUSTRI SEBAGAI AGEN FITOREMEDIASI CEMARAN LOGAM BERAT ASAL ABU TERBANG

Analysis of the ability of industrial forest plants such as A. crassicarpa and A. mangium can act as phytoremediation agents in absorbing heavy metals (Cd, Co and Pb) contained in coal-burning fly ash boilers that can be used as peat soil ameliorants. Provision of fly ash on peat soils has potential to pollute the soil. The purpose of this study was to analyze the effectiveness of forest indutrial plants in reducing heavy metal content in soils contaminated with fly ash and to estimate how much economic valuation is valued for the phytoremediation ability of acacia plants in the case of heavy metal contamination from pulp industry activities and paper.The results of the analysis of the ability of industrial forest plants in absorbing heavy metals show that A. crassicarpa is better at absorbing Pb metal, A. mangium is better at absorbing Co metals and Cd metal showed no significant difference between the two types of industrial forest plants

Optimal Allocation Model of Plant Community in Slope Ecological Restoration

<p>In order to effectively analyze the optimal allocation mode of plant community in the process of slope ecological restoration, 15 plants were selected and 10 plant community combinations were designed. The experiment lasted for 90 days, and the optimal allocation model of plant community was obtained. The optimal allocation pattern of plant communities in slope ecological restoration was C7: 10 plants of Amorpha fruticosa + 20 plants of Vitex negundo + 30 plants of Festuca arundinacea + 20 plants of Moxa argyi + 10 plants of Dianthus caryophylla + 20 plants of Lespedeza + 10 plants of Leucaena + 8 plants of Acacia; C8: 30 plants of Euonymus microphylla + 10 plants of Shearnut + 20 plants of Alfalfa + 30 plants of Pigeon Beans + 20 plants of Crotalaria mucronata + 20 plants of Mimosa bimucronata; C3: 20 plants of Amorpha fruticosa + 50 plants of Euonymus fortunei var. radicans + 20 plants of Aicao + 10 plants of Pigeon bean + 8 plants of Leucaena +10 plants of Dodonaea viscosa. The experimental results show that the optimal allocation model of plant community is effective in slope ecological restoration.</p>

The effectiveness of Acacia oral gel on wound healing in rabbits clinically and histopathologically

Oral mucosa considered as one of defensive mechanism of oral cavity, it is important in oral functions as eating, swallowing and even talking. Any wound or discontinuity effects on these functions so speeding the healing process with less inflammatory reactions is crucial, many herbs were used at different eras to reach this goal one of these herbs is Acacia plant. The aim of this study is to evaluate the effect of acacia oral gel extract on the wound healing. In materials and method This study include (twenty-one) male rabbits, (seven) for each group used for this study, two incisions of one centimeter length were made on buccal oral mucosa bilaterally one is treated by acacia oral gel while other left for normal healing, the follow up of the wound at twenty-four hours, three days and seven days clinically and histopathological examination were done. In result of this study, The study shows decrease in inflammatory reaction after seventy-two hours of the wound and increase in re-epithelialization rate in wound healing of acacia nilotica gel group, So it is concluded from this study that the acacia plant gel extract has anti-inflammatory effect and accelerate the wound healing of oral mucosa in rabbits.

Physical and Mechanical Properties of Fast Growing Polyploid Acacia Hybrids (A. auriculiformis × A. mangium) from Vietnam

Acacia plants are globally important resources in the wood industry, but particularly in Southeast Asian countries. In the present study, we compared the physical and mechanical properties of polyploid Acacia (3x and 4x) clones with those of diploid (2x) clones grown in Vietnam. We randomly selected 29 trees aged 3.8 years from different taxa for investigation. BV10 and BV16 clones represented the diploid controls; X101 and X102 were the triploid clones; and AA-4x, AM-4x, and AH-4x represented neo-tetraploid families of Acacia auriculiformis, Acacia mangium, and their hybrid clones. The following metrics were measured in each plant: stem height levels, basic density, air-dry equilibrium moisture content, modulus of rupture (MOR), modulus of elasticity (MOE), compression strength, and Young’s modulus. We found that the equilibrium moisture content significantly differed among clones, and basic density varied from pith-to-bark and in an axial direction. In addition, the basic density of AA-4x was significantly higher than that of the control clones. Furthermore, the MOR of AM-4x was considerably lower than the control clones, whereas the MOE of X101 was significantly higher than the control values. The compression strength of AM-4x was significantly lower than that of the control clones, but AH-4x had a significantly higher Young’s modulus. Our results suggest that polyploid Acacia hybrids have the potential to be alternative species for providing wood with improved properties to the forestry sector of Vietnam. Furthermore, the significant differences among the clones indicate that opportunities exist for selection and the improvement of wood quality via selective breeding for specific properties.

Phenolic profiling, biological activities and in silico studies of Acacia tortilis (Forssk.) Hayne ssp. raddiana extracts

To evaluate the phenolic profile and biological activity of the Algerian Sahara plant Acacia tortilis (Forssk.) Hayne ssp. raddiana decoction and 80% ethanolic extracts were studied. Chromatographic profiling indicated the presence of 36 phenolic compounds, including gallic acid esterified derivatives, galloylquinic derivatives and flavan-3-ols galloyl derivatives. Both extracts showed significant cytotoxic activity and a potent anti-inflammatory activity. They were effective against several multi-drug resistant bacteria, namely methicillin-resistant Staphylococcus aureus (MRSA). To understand the possible mechanism of action of MRSA inhibition activity, an in silico docking analysis was done using a virtual library of the 36 determined phenolic compounds against penicillin-binding protein (PBP2a), a protein known to be involved in MRSA resistance to β-lactam antibiotics. A. tortilis extracts showed interesting biological activities and the phenolic compounds found could be an interesting starting point for the development of cytotoxic and anti-inflammatory drugs and especially anti-MRSA antibiotics.

Survival of Acacia leucophloea and Pterocarpus indicus Under Canopy of Acacia nilotica as Nurse Plants

Acacia nilotica which is a plants of the genus Acacia which is known as one of the invasive genera. A. nilotica is very invasive due to several advantages it has such as very large seed production, seed dispersal far enough by wildlife, young plants to grow very fast and are resistant to drought and fire. Some invasive acacia species can act as nurse plants for native plants, which increases the photosynthetic efficiency of native plants, and protects native species from extreme temperatures. Therefore, it is important to know the chance of recolonizing native plants in areas that are invaded by foreign species, by looking at the performance of plants seedlings under A. nilotica stands. This study aims to determine the potential of A. nilotica invasive plants as nurse plants for local plants in East Timor. The stages in this research are the preparation of tools and materials, seeding of target species, selection of A. nilotica stands as a nurse plants, selection of healthy target species seedlings to be planted, planting of target species seedlings under A. nilotica stands, observing survival ability and species growth target. An analysis of the survival ability of the seedlings was carried out using the Kaplan-Meier survival curve. The results of this study are the angsana seedlings and kabesak in this study experienced many deaths, external factors such as high-temperature factors due to the long dry season suspected to be the cause. A. nilotica shade does not affect the survival of the angsana and kabesak seedlings, allelopathy produced by A. nilotica plants accompanied by a drought in a long period of time causes the plants is unable to act as nurse plants for kabesak and angsana plants.