The unique variety of soil enzymes found in termite mound soil results in different physico-chemical properties than the surrounding field soil. Metagenomic research of termite mound soil samples has paved a critical path in our comprehension of the ecosystem's diverse bacterial species. Despite advanced soil research, basic analytical tests on termite mound soil have not been considered. In the present study, microbial diversity of the two-termite mound soil was studied using Illumina Miseq sequencing by targeting V3-V4 regions in 16S rRNA. In termite mound soil (TMS) and adjacent field soil (AFS), the maximum bulk density, particle density, and porosity were 1.82 mg m−3, 3.33 mg m−3, 65 % and 1.25 mg m−3, 2.5 mg m−3, 50 %, respectively. The available NPK status in termite mound soil was higher than in surrounding field soil. The most abundant phylum in both samples was Proteobacteria, with abundances of 22.83 % and 32.76 % for samples R1 and R2, respectively. A noteworthy finding is that a small portion of the relative abundance is classified in the Kingdom Archaea identified upto species level of taxonomic classification.