Abundance Of Heterotrophic Bacteria Research Articles

Metagenomic analysis reveals abundance of mixotrophic, heterotrophic, and homoacetogenic bacteria in a hydrogen-based membrane biofilm reactor

Heterotrophic microorganisms are frequently observed in hydrogenotrophic denitrification systems and are presumed to contribute to their improved performance. However, their roles and metabolic pathways in the hydrogen-based membrane biofilm reactor (H2−MBfR) system remain unclear. The objective of this study was to elucidate the underlying mechanisms driving heterotrophic denitrification. For this purpose, metagenomic analysis was conducted on an H2−MBfR showing higher denitrification performance, focusing on the metabolic function of the microbial community. Functional genes related to H2 oxidation, organic carbon metabolism, and denitrification were the major targets of interest. This analysis revealed a substantial number of genes associated with the oxidation of organic carbon compounds in the biofilm, suggesting its potential for heterotrophic denitrification. Investigation of the genes of interest in metagenome-assembled genomes (MAGs) has demonstrated a predominance of mixotrophs or heterotrophs rather than obligate autotrophs. Notably, MAGs exhibiting the highest abundance of genes of interest were affiliated with Hydrogenophaga and Thauera, implying their significant role in denitrifying the H2−MBfR as mixotrophs utilizing both H2 and organic substrates. The identification of 11 MAGs, presumed to originate from homoacetogens suggested that acetate might contribute to the proliferation of heterotrophs. Based on these metagenomic findings, possible metabolic pathways were identified to explain heterotrophic denitrification within the H2−MBfR biofilms.

Protozoan communities serve as a strong indicator of water quality in the Nile River

The relationship between the protozoan communities and environmental variables was studied in the Nile River to evaluate their potential as water quality indicators. Protozoans were sampled monthly at six sampling sites in the Nile's Damietta Branch across a spatial gradient of environmental conditions during a 1-year cycle (February 2016–January 2017). The Protozoa community was comprised of 54 species belonging to six main heterotrophic Protozoa phyla. The abundance (average, 1089 ± 576.18 individuals L−1) and biomass (average, 86.60 ± 106.13 μg L−1) were comparable between sites. Ciliates comprised the majority of protozoan species richness (30 species), abundance (79.72%), and biomass (82.90%). Cluster analysis resulted in the distribution of protozoan species into three groups, with the most dominant species being the omnivorous ciliate Paradileptus elephantinus. Aluminium, fluoride, and turbidity negatively affected abundance and biomass, while dissolved oxygen and potassium positively impacted biomass. Of the dominant species recorded over the study area, the amoebozoa Centropyxis aculeata was associated with runoff variables, while the bacterivorous ciliates Colpidium colpoda, Glaucoma scintillans, and Vorticella convallaria were related to the abundance of heterotrophic bacteria, phytoplankton biomass, and total organic carbon. Total dissolved salts, PO4, NH3, NO2, dissolved oxygen, and total organic carbon were the strongest causative factors for protozoa distribution. The α-Mesosaprobic environment at site VI confirmed a high load of agricultural runoffs compared to other sites. This study demonstrates that protozoans can be a potential bioindicator of water quality status in this subtropical freshwater river system.

Spatial and diel variations of bacterioplankton and pico-nanoeukaryote communities and potential biotic interactions during macroalgal blooms

We investigated spatial heterogeneity and diel variations in bacterioplankton and pico-nanoeukaryote communities, and potential biotic interactions at the extinction stage of the Ulva prolifera bloom in the Jiaozhou Bay, Yellow Sea. It was found that the presence of Ulva canopies significantly promoted the cell abundance of heterotrophic bacteria, raised evenness, and altered the community structure of bacterioplankton. A diel pattern was solely significant for pico-nanoeukaryote community structure. >50 % of variation in the heterotrophic bacterial abundance was accounted for by the ratio of Bacteroidota to Firmicutes, and dissolved organic nitrogen effectively explained the variations in cell abundances of phytoplankton populations. The factors representing biotic interactions frequently contributed substantially more than environmental factors in explaining the variations in diversity and community structure of both bacterioplankton and pico-nanoeukaryotes. There were higher proportions of eukaryotic pathogens compared to other marine systems, suggesting a higher ecological risk associated with the Ulva blooms.

Interaction between phytoplankton and heterotrophic bacteria in Arctic fjords during the glacial melting season as revealed by eDNA metabarcoding.

The hydrographic variability in the fjords of Svalbard significantly influences water mass properties, causing distinct patterns of microbial diversity and community composition between surface and subsurface layers. However, surveys on the phytoplankton-associated bacterial communities, pivotal to ecosystem functioning in Arctic fjords, are limited. This study investigated the interactions between phytoplankton and heterotrophic bacterial communities in Svalbard fjord waters through comprehensive eDNA metabarcoding with 16S and 18S rRNA genes. The 16S rRNA sequencing results revealed a homogenous community composition including a few dominant heterotrophic bacteria across fjord waters, whereas 18S rRNA results suggested a spatially diverse eukaryotic plankton distribution. The relative abundances of heterotrophic bacteria showed a depth-wise distribution. By contrast, the dominant phytoplankton populations exhibited variable distributions in surface waters. In the network model, the linkage of phytoplankton (Prasinophytae and Dinophyceae) to heterotrophic bacteria, particularly Actinobacteria, suggested the direct or indirect influence of bacterial contributions on the fate of phytoplankton-derived organic matter. Our prediction of the metabolic pathways for bacterial activity related to phytoplankton-derived organic matter suggested competitive advantages and symbiotic relationships between phytoplankton and heterotrophic bacteria. Our findings provide valuable insights into the response of phytoplankton-bacterial interactions to environmental changes in Arctic fjords.

Do weathered microplastics impact the planktonic community? A mesocosm approach in the Baltic Sea

Microplastics (MPs) are ubiquitous pollutants of increasing concern in aquatic systems. However, little is still known about the impacts of weathered MPs on plankton at the community level after long-term exposure. In this study, we investigated the effects of weathered MPs on the structure and dynamics of a Baltic Sea planktonic community during ca. 5 weeks of exposure using a mesocosm approach (2 m3) mimicking natural conditions. MPs were obtained from micronized commercial materials of polyvinyl chloride, polypropylene, polystyrene, and polyamide (nylon) previously weathered by thermal ageing and sunlight exposure. The planktonic community was exposed to 2 μg L-1 and 2 mg L-1 of MPs corresponding to measured particle concentrations (10–120 μm) of 680 MPs L-1 and 680 MPs mL-1, respectively. The abundance and composition of all size classes and groups of plankton and chlorophyll concentrations were periodically analyzed throughout the experiment. The population dynamics of the studied groups showed some variations between treatments, with negative and positive effects of MPs exhibited depending on the group and exposure time. The abundance of heterotrophic bacteria, pico- and nanophytoplankton, cryptophytes, and ciliates was lower in the treatment with the higher MP concentration than in the control at the last weeks of the exposure. The chlorophyll concentration and the abundances of heterotrophic nanoflagellates, Astromoeba, dinoflagellate, diatom, and metazooplankton were not negatively affected by the exposure to MPs and, in some cases, some groups showed even higher abundances in the MP treatments. Despite these tendencies, statistical analyses indicate that in most cases there were no statistically significant differences between treatments over the exposure period, even at very high exposure concentrations. Our results show that weathered MPs of the studied conventional plastic materials have minimal or negligible impact on planktonic communities after long-term exposure to environmentally relevant concentrations.

Evaluation of Microbiological and Chemical Properties of Soils as a Result of Anthropogenic Denudation

Excessive agricultural intensification adversely affects soil quality, particularly in hilly terrain, leading to increased erosion. Anthropogenic denudation, intensified by tillage erosion, results in the displacement of soil material from hilltops and shoulders to their bases. The research hypothesis posits that tillage erosion adversely affects the microbiological and chemical properties of soils, especially at the hilltops of intensively cultivated areas. The study aimed to assess the microbiological and chemical properties of Luvisols cultivated under conventional plowing in the moraine region of the Southern Krajna Lakeland, Poland. The evaluation focused on the results of soil sample analyses taken from the hilltops and foothills of eroded mounds. Microbiological investigations included determining the abundance of actinomycetes, filamentous fungi, heterotrophic bacteria, cellulolytic microorganisms, copiotrophs, and oligotrophs. Additionally, pH values and the contents of phosphorus, potassium, magnesium, total organic carbon, and nitrogen were determined. A higher abundance of bacteria, actinomycetes, and copiotrophs was observed at the foothills. Statistically significant differences due to slope effects were noted for all chemical parameters, with higher concentrations of organic carbon, nitrogen, potassium, and phosphorus found in the foothill areas. Understanding denudation processes can contribute to sustainable soil resource use and agrocenosis conservation.

Ultra-stable mixotrophic denitrification coupled with anammox under organic stress for mainstream municipal wastewater treatment

Sulfur-based autotrophic denitrification (SAD) coupled with anammox is a promising process for autotrophic nitrogen removal in view of the stable nitrite accumulation during SAD. In this study, a mixotrophic nitrogen removal system integrating SAD, anammox and heterotrophic denitrification was established in a single-stage reactor. The long-term nitrogen removal performance was investigated under the intervention of organic carbon sources in real municipal wastewater. With the shortening of hydraulic retention time, the nitrogen removal rate of the mixotrophic system dominated by the autotrophic subsystem reached 0.46 Kg N/m³/d at an organic loading rate of 0.57 Kg COD/m³/d, with COD and total nitrogen removal efficiencies of 82.5 % and 94 %, respectively, realizing an ideal combination of autotrophic and heterotrophic systems. The 15NO3−-N isotope labeling experiments indicated that thiosulfate-driven autotrophic denitrification was the main pathway for nitrite supply accounting for 80.6 %, while anammox exhibited strong competitiveness for nitrite under the dual electron supply of sulfur and organic carbon sources and contributed to 65.1 % of nitrogen removal. Sludge granulation created differential functional distributions in different forms of sludge, with SAD showing faster reaction rate as well as higher nitrite accumulation rate in floc sludge, while anammox was more active in granular sludge. Real-time quantitative PCR, RT-PCR and high-throughput sequencing results revealed a dynamically changing community composition at the gene and transcription levels. The decrease in heterotrophic denitrification bacteria abundance indicated the effectiveness of the operational strategy for introduction of thiosulfate and maintaining the dominance of SAD in denitrification process in suppressing the excessive growth of heterotrophic bacteria in the mixotrophic system. The high transcriptional expression of sulfur-oxidizing bacteria (SOB) (Thiobacillus and Sulfurimonas) and anammox bacteria (Candaditus_Brocadia and Candidatus_Kuenenia) played a crucial role in the stable nitrogen removal.

Simultaneously enhanced autotrophic–heterotrophic denitrification in iron-based ecological floating bed by plant biomass: Metagenomics insights into microbial communities, functional genes and nitrogen metabolic pathways

In this study, the ecological floating bed supporting with zero-valent iron (ZVI) and plant biomass (EFB-IB) was constructed to improve nitrogen removal from low-polluted water. The effects of ZVI coupling with plant biomass on microbial community structure, metabolic pathways and functional genes were analyzed by metagenomic sequencing, and the mechanism for nitrogen removal was revealed. Results showed that compared with mono-ZVI system (EFB-C), the denitrification efficiencies of EFB-IB were effectively enhanced, with the higher average NO3−-N removal efficiencies of 22.60–59.19%. Simultaneously, the average NH4+-N removal efficiencies were 73.08–91.10%. Metagenomic analyses showed that EFB-IB enriched microbes that involved in iron cycle, lignocellulosic degradation and nitrogen metabolism. Plant biomass addition simultaneously increased the relative abundances of autotrophic and heterotrophic denitrifying bacteria. Network analysis showed the cooperation between autotrophic and heterotrophic denitrifying bacteria in EFB-IB. Moreover, compared with EFB-C, plant biomass addition increased the relative abundances of genes related to iron cycle, lignocellulose degradation and glycolysis processes, ensuring the production of autotrophic and heterotrophic electron donors. Therefore, the relative abundances of key enzymes and functional genes related to denitrification were higher in EFB-IB, being beneficial to the NO3−-N removal. Additionally, the correlation analysis of nitrogen removal and functional genes verified the synergistic mechanism of iron-based autotrophic denitrification and plant biomass-mediated heterotrophic denitrification in EFB-IB. In summary, plant biomass has excellent potential to improve the nitrogen removal of iron-based EFB from low-polluted water.

Application of alkali-heated corncobs enhanced nitrogen removal and microbial diversity in constructed wetlands for treating low C/N ratio wastewater.

Lack of carbon source is the main limiting factor in the denitrification of low C/N ratio wastewater in the constructed wetlands (CWs). Agricultural waste has been considered as a supplementary carbon source but research is still limited. To solve this problem, ferric carbon (Fe-C) + zeolite, Fe-C + gravel, and gravel were used as substrates to build CWs in this experiment, aiming to investigate the effects of different carbon sources (rice straw, corncobs, alkali-heated corncobs) on nitrogen removal performance and microbial community structure in CWs for low C/N wastewater. The results demonstrated that the microbial community and effluent nitrogen concentration of CWs were mainly influenced by the carbon source rather than the substrate. Alkali-heated corncobs significantly enhanced the removal of NO2--N, NH4+-N, NO3-N, and TN. Carbon sources addition increased microbial diversity. Alkali-heated corncobs addition significantly increased the abundance of heterotrophic denitrifying bacteria (Proteobacteria and Bacteroidota). Furthermore, alkali-heated corncobs addition increased the copy number of nirS, nosZ, and nirK genes while greenhouse gas fluxes were lower than common corncobs. In summary, alkali-heated corncobs can be considered as an effective carbon source.

Growth performance and physiological responses of striped catfish (Pangasianodon hypophthalmus) under different carbohydrates supplemented biofloc aquaculture systems

Biofloc technology is booming in inland aquaculture sector across the world especially in the places where water scarcity is high and land cost is expensive. Currently, the lack of appropriate selection and application feasibility of suitable carbohydrate in water quality management and fish health in biofloc aquaculture system are the major concern for wider adaptability of the technology. Therefore, the present experiment investigated the effect of different carbohydrate sources on overall wellbeing of striped catfish (Pangasianodon hypophthalmus) fingerlings. The experiment was randomly designed in four treatments (T1, T2, T3 and T4) and one control in triplicates in indoor tanks (1.0 m3). T1, T2, T3 and T4 groups supplemented with tapioca flour, sugarcane bagasse, molasses and jaggery, respectively under biofloc system at C:N ratio of 15:1, whereas the control group was not supplemented with carbohydrate source. Pre-acclimatized 525 fingerlings (7.2 ± 0.03 cm, 7.4 ± 0.003 g) were randomly stocked (35 nos. m−3) in the tanks and reared for 90 days. Overall, better water quality, fish performance and health status were observed in all the carbohydrates supplemented treatments than the control. Higher abundance of heterotrophic bacteria (5.88–118.18%) and biofloc associated microorganisms in jaggery supplemented biofloc, significantly (P ≤ 0.05) reduced total ammonia nitrogen, nitrite‑nitrogen, and increased nitrate‑nitrogen concentrations than other treatments. In the T4 treatment, jaggery supplementation enhanced biofloc volume, which influenced enhancement of fish body weight gain, specific growth rate, and improvement of feed conversion ratio than other biofloc treatments. Enhanced gut microbial population and digestive enzymes activity in fish significantly enhanced protein efficiency ratio, apparent net protein utilization, and flesh protein content in the T4 treatment. Fish showed significantly (P ≤ 0.05) higher haematological, immunological, and improved antioxidative responses in the T4 treatment than other treatments. Consumption of biofloc significantly (P ≤ 0.05) improved intestinal villi microarchitecture as higher villi surface area, width and height were detected in the T4 treatment. Collectively, the findings suggested that jaggery was the suitable carbohydrate source in terms of overall improvement of the culture system including growth performance and health status of P. hypophthalmus, if applied appropriately in biofloc aquaculture system. The present study comprehensively addressed carbohydrate sources, biofloc formation, total ammonia nitrogen management, fish growth and health resulting in better adoptability of biofloc technology by farmers in aquaculture.

Stable microbial community in compacted bentonite after 5 years of exposure to natural granitic groundwater.

The Materials Corrosion Test (MaCoTe) at the Underground Research Laboratory in Grimsel, Switzerland, assesses the microbiology and corrosion behavior of engineered barrier components of a deep geological repository (DGR) for long-term disposal of high-level nuclear waste. Diversity and temporal changes of bentonite-associated microbial community profiles were assessed under DGR-like conditions for compacted Wyoming MX-80 bentonite (1.25 g/cm3 and 1.50 g/cm3 targeted dry densities) exposed to natural groundwater. Using culture-dependent and molecular techniques, samples taken from the outside layer of 5-year borehole modules revealed up to 66% and 23% of 16S rRNA gene sequences affiliated with Desulfosporosinus and Desulfovibrio, respectively. Putatively involved in sulfate reduction, these taxa were almost undetectable within the bentonite core. Instead, microbial profiles of the inner bentonite core were similar to uncompacted bentonite used to pack modules years earlier, and were consistent with a previously published 1-year time point, revealing no detectable microbial growth. Abundances of culturable aerobic and anaerobic heterotrophic bacteria in the uncompacted bentonite were relatively low, with less than 1,000 and 100 colony-forming units (CFUs) per gram dry weight, respectively. Nearly 5 years after emplacement, culturable heterotrophic bacterial CFUs and sulfate-reducing bacteria did not change significantly inside the bentonite core. Phospholipid fatty acid data indicated similar lipid abundance, and corresponding cell abundance estimates, for inner 5-year MaCoTe bentonite samples compared to those previously obtained for 1-year incubations. Collectively, our results provide complementary evidence for microbial stability inside highly compacted bentonite exposed to conditions that mimic engineered barrier components of a deep geological repository. IMPORTANCE The long-term safety of a deep geological repository for used nuclear fuel is dependent on the performance of the engineered and natural barriers. Microbial activity can produce chemical species that can influence the corrosion of the disposal containers for used nuclear fuel. Although previous studies have evaluated the microbiology of compacted bentonite clay within subsurface environments, these have been limited to relatively short incubations (i.e., 1 year). The current study provides a unique 5-year perspective that reinforces previous findings of growth inhibition for bentonite clay exposed to in situ subsurface conditions.

Saltwater Intrusion Impacts Microbial Diversity and Function in Groundwater Ecosystems

Background: Groundwater ecosystem services provided by microbial communities are essential for the maintenance of water quality. For example, nitrate contamination is a recognised health and ecosystem issue in most groundwater systems, often alleviated through microbial processes. The effects of climate change, including increasing salinity from rising sea levels, or over-abstraction, on these communities are largely unknown. Methods: This study uses a combination of culture-dependent (growth curves, isolation of bacteria) and culture-independent (16S rRNA gene sequencing) methods to identify the potential effects of saltwater intrusion on groundwater microbes and their ecosystem functions. Results: Some groundwater microbial communities are negatively impacted by increasing chloride concentrations, including declines in bacteria responsible for nitrate and ammonia removal. These ecosystems should be prioritised for future protection from sea level rise or increased extraction of groundwater for agriculture and other uses. Other microbial communities are stimulated in the presence of chloride, often caused by an increase in abundance of salt-tolerant heterotrophic bacteria using sugars, peptides, or organic acids for energy. Conclusion: There have been no previous studies investigating the impact of chloride on Aotearoa New Zealand groundwaters. The identification of keystone species that are affected by increasing salinity, which have a disproportionately large effect on the ecosystem and low functional redundancy, is essential. Water management decisions about future abstraction limits and defences against sea level rise can be underpinned by robust scientific knowledge about microbial community sensitivity to salinity.

Soil phosphorus drives variation in diazotrophic communities in a subtropical nitrogen-rich forest

Although nitrogen (N)-fixing bacteria (diazotrophs) play a vital role in the biosphere's N cycle. However, large uncertainty exists regarding how atmospheric N deposition regulates biological N fixation (BNF) in soils and which diazotrophic taxa are responsible for this process. We targeted the nifH gene to investigate the effects of long-term N addition on the abundance and composition of diazotrophic communities in subtropical N-rich forests, using real-time quantitative polymerase chain reaction (q-PCR) and MiSeq sequencing. We conducted a field trial for seven years and included three N treatment levels (+0, +50, +150 kg N ha−1 yr−1; CK, LN, HN). With increasing N addition, the average total P concentration in the whole soil profile and the topsoil P availability decreased significantly, further reducing nitrogenase activity and nifH abundance significantly, while increasing the diversity of the diazotrophic community. After seven years of N addition, the relative abundance of uncultured Cyanobacteria significantly decreased, while those of Rhizobiales and Desulfobulbaceae significantly increased. The diazotrophic communities transitioned from free-living N fixers (uncultured Cyanobacteria-dominated) to symbiotic N fixers (Rhizobiales-dominated; HN treatment). The relative abundance of heterotrophic diazotrophic bacteria and the occasional diazotrophic species increased, resulting in diazotrophic bacteria still maintaining high competitiveness in the N-rich and/or P-limited soils. Proteobacteria (e.g., Rhizobiales) had the highest potential to participate in microbial mechanisms triggered by P scarcity. Variation partitioning analyses showed that diazotrophic community composition and diversity were mainly correlated with the soil P availability, with soil AP having the highest individual effect. Overall, this study will broaden our understanding of N fixation mechanisms and the diazotrophic community variation in N-rich forests, while also highlighting soil P as a key factor driving changes in diazotrophic communities.

Succession of bacterial biofilm communities following removal of chloramine from a full-scale drinking water distribution system

Monochloramine is used to regulate microbial regrowth in drinking water distribution systems (DWDS) but produces carcinogenic disinfection byproducts and constitutes a source of energy for nitrifying bacteria. This study followed biofilm-dispersed microbial communities of a full-scale DWDS distributing ultrafiltered water over three years, before and after removal of monochloramine. Communities were described using flow cytometry and amplicon sequencing, including full-length 16S rRNA gene sequencing. Removal of monochloramine increased total cell counts by up to 440%. Increased abundance of heterotrophic bacteria was followed by emergence of the predatory bacteria Bdellovibrio, and a community potentially metabolizing small organic compounds replaced the nitrifying core community. No increased abundance of Mycobacterium or Legionella was observed. Co-occurrence analysis identified a network of Nitrosomonas, Nitrospira, Sphingomonas and Hyphomicrobium, suggesting that monochloramine supported this biofilm community. While some species expanded into the changed niche, no immediate biological risk to consumers was indicated within the DWDS.

The use of an algal bioindicator in the assessment of different chemical remediation strategies for PAH-contaminated soils and sediments

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous petroleum-derived pollutants that tend to accumulate in soils and sediments. Among the in situ chemical oxidation technologies applied at PAH-contaminated sites are Fenton reagent (FN), persulfate ion (PS) and permanganate ion (PM). The natural phytoplankton of an aquatic environment is useful as a biological indicator of the treatment efficacy of pollutant degradation. The objective of this work was to assess the use of an algal species, native from Los Barreales Reservoir (Argentina), as an efficacy indicator of the chemical remediation of PAH-contaminated soils and sediments. Oxidations with FN, PS or PM were carried out on soils and sediments previously contaminated with two types of PAHs (phenanthrene (Ph) or Benzo(a)anthracene (Ba)), analysing pH, residual oxidant, PAH degradation and heterotrophic bacteria abundance in soils or sediments after 7 days. Then, a bioassay was carried out by incubating the obtained soils or sediments, and the native alga Scenedesmus quadricauda (Turpin, Brébisson), analysing its abundance after 7 days. S. quadricauda abundance was related to PAH degradation efficacy, residual oxidants and changes in system pH. In this sense, the application of PS produced the lowest growth of the indicator in assays both with soils and sediments. Fenton reaction results varied depending on the contaminating PAH, with the best percentages of PAH degradation and growth of S. quadricauda for Ba. PM oxidation resulted in the best development of the indicator. This study constitutes a first approach to the use of S. quadricauda as an efficiency indicator of soil/sediment chemical remediation.

Huge anthropogenic microbial load during southwest monsoon season in coastal waters of Kakinada, Bay of Bengal

To examine the influence of anthropogenic activities on the marine ecosystem near the coastal waters of the port city, Kakinada, a study was conducted to investigate the abundance of heterotrophic, indicator and pathogenic bacteria during the spring inter monsoon (SIM) and southwest monsoon (SWM) seasons. A drastic change in the marine bacteria due to the input of allochthonous bacteria during SWM was noticed. An order of magnitude higher abundance of indicators (Escherichia coli and Enterococcus faecalis) and bacterial pathogens (Proteus mirabilis and Pseudomonas aeruginosa) was observed during SWM. In contrast, Chlorophyll-a, heterotrophic bacterial abundance, Aeromonas hydrophila and Klebsiella pneumoniae were higher during SIM. A significant increase in some of the indicator and pathogenic bacterial abundance due to moderate rainfall suggests that the improper drainage system in the city could spread these bacteria, posing a considerable threat to both environment and human health.

Effect of Microbial Preparation and Biomass Incorporation on Soil Biological and Chemical Properties

In order to meet the global nutritional needs of a growing population, attempts are being made to develop strategies that can effectively offset the negative effects of intensive farming. The aim of this study was to investigate the effect of Effective Microorganisms (EM) preparation and organic matter incorporation on the soil microbiological and chemical features. The analyses included the number of heterotrophic bacteria, fungi, actinobacteria, cellulolytic, amylolytic and proteolytic microorganisms, and bacteria of the genus Azotobacter. The content of organic carbon, the total and mineral nitrogen forms of phosphorus, potassium, magnesium, and the pH were also determined. The application of an EM of higher dose combined with the manure and straw resulted in the highest abundance of heterotrophic bacteria (165.1 × 106 cfu g−1), actinobacteria (43.2 × 105 cfu g−1), cellulolytic (17.2 × 106 cfu g−1), and proteolytic bacteria (82.0 × 106 cfu g−1). The highest content of chemical parameters was always observed in the experimental variant, including biomass incorporation, accompanied by EM use. The novelty of our research is the analysis of the synergistic effect of the experimental factors studied on the microbiological and chemical parameters of arable soils.

Short-term dynamics of nano- and picoplankton production in an embayment of the southern Benguela upwelling region

In the southern Benguela ecosystem, regenerated production and high levels of organic matter remineralisation are expected to dominate during periods of relaxation. To study microbial growth and productivity under these remineralising conditions, we measured size fractionated (micro-nanoplankton: 10–200 μm and nano-picoplankton: 0.3–10 μm) net primary production, uptake rates of nitrate, ammonium, and urea, as well as nano- and picoplankton community composition and biomass over five consecutive days in autumn (March 2018). Samples were collected from three depths (1 m, 25 m, and 50 m) at a single station in St Helena Bay and abundances of nanophytoplankton, picophytoplankton and heterotrophic bacteria were determined using flow cytometry. There were differences in productivity among days but depth-differentiation was more apparent in the rates of net primary production and nitrogen uptake, with the highest rates (rate ± SD) (NPP: 3.36 ± 1.82 μmol L−1 d−1; ρNO3-: 0.30 ± 0.18 μmol L−1 d−1; ρNH4+: 2.27 ± 0.75 μmol L−1 d−1; ρUrea: 1.38 ± 0.02 μmol L−1 d−1) measured at the surface. Ammonium and urea uptake rates were two-to six-fold higher than those of nitrate, indicating that most of the biomass was produced through regenerated production with f-ratios of 0.02–0.21. Nano-picoplankton comprised 67% of the carbon biomass and were responsible for 90% of net primary production and 79–85% of total nitrogen (i.e., nitrate + ammonium + urea) uptake. Small cell size likely conferred advantages on nano-picoplankton in the nutrient-deplete euphotic zone and the low-oxygen (<89.3 μmol L−1) conditions at 25 m and 50 m. Nitrite oxidation rates were fastest at deeper depths where heterotrophic bacteria were most abundant. Heterotrophic bacteria also contributed the most (95%) carbon biomass at all depths, suggesting a major role for these microorganisms in carbon and nitrogen cycling throughout the water column of St Helena Bay.

Simultaneous use of nitrate and calcium peroxide to control sulfide and greenhouse gas emission in sewers

The sewer system is a significant source of hydrogen sulfide (H2S) and greenhouse gases which has attracted extensive interest from researchers. In this study, a novel combined dosing strategy using nitrate and calcium peroxide (CaO2) was proposed to simultaneously control sulfide and greenhouse gases, and its performance was evaluated in laboratory-scale reactors. Results suggested that the addition of nitrate and CaO2 improved the effectiveness of sulfide control. And the combination index method further proved that nitrate and CaO2 were synergistic in controlling sulfide. Meanwhile, the combination of nitrate and CaO2 substantially reduced greenhouse gas emissions, especially the carbon dioxide (CO2) and methane (CH4). The microbial analysis revealed that the combined addition greatly stimulated the accumulation of nitrate reducing-sulfide oxidizing bacteria (NR-SOB) that participate in anoxic nitrate-dependent sulfide oxidation, while the abundance of heterotrophic denitrification bacteria (hNRB) was reduced significantly. Moreover, the presence of oxygen and alkaline chemicals generated by CaO2 facilitated the inhibition of sulfate-reducing bacteria (SRB) activities. Therefore, the nitrate dosage was diminished significantly. On the other hand, the generated alkaline chemicals promoted CO2 elimination and inhibited the activities of methanogens, leading to a decrease of CO2 and CH4 fluxes, which facilitated elimination of greenhouse effects. The intermittent dosing test showed that the nitrate and CaO2 could be applied intermittently for sulfide removal. And the chemical cost of intermittent dosing strategy was reduced by 85 % compared to the continuous dosing nitrate strategy. Therefore, intermittent dosing nitrate combined with CaO2 is probably an effective and economical approach to control sulfide and greenhouse gases in sewer systems.

Abundance of heterotrophic marine bacteria, Vibrio, and marine fungi in green seaweed Caulerpa racemosa in Sibutu, Tawi-Tawi, Philippines

Abundance of heterotrophic marine bacteria, Vibrio, and marine fungi in green seaweed Caulerpa racemosa in Sibutu, Tawi-Tawi, Philippines