ABTS Radical Cation Decolorization Assay Research Articles

Novel Multi-Target Agents Based on the Privileged Structure of 4-Hydroxy-2-quinolinone.

In this work, the privileged scaffold of 4-hydroxy-2quinolinone is investigated through the synthesis of carboxamides and hybrid derivatives, as well as through their bioactivity evaluation, focusing on the ability of the molecules to inhibit the soybean LOX, as an indication of their anti-inflammatory activity. Twenty-one quinolinone carboxamides, seven novel hybrid compounds consisting of the quinolinone moiety and selected cinnamic or benzoic acid derivatives, as well as three reverse amides are synthesized and classified as multi-target agents according to their LOX inhibitory and antioxidant activity. Among all the synthesized analogues, quinolinone-carboxamide compounds 3h and 3s, which are introduced for the first time in the literature, exhibited the best LOX inhibitory activity (IC50 = 10 μM). Furthermore, carboxamide 3g and quinolinone hybrid with acetylated ferulic acid 11e emerged as multi-target agents, revealing combined antioxidant and LOX inhibitory activity (3g: IC50 = 27.5 μM for LOX inhibition, 100% inhibition of lipid peroxidation, 67.7% ability to scavenge hydroxyl radicals and 72.4% in the ABTS radical cation decolorization assay; 11e: IC50 = 52 μM for LOX inhibition and 97% inhibition of lipid peroxidation). The in silico docking results revealed that the synthetic carboxamide analogues 3h and 3s and NDGA (the reference compound) bind at the same alternative binding site in a similar binding mode.

In Vitro Antioxidants Activities of Extract of Tri-Kasorn-Mas remedy and its plant ingredients

Tri-Kasorn-Mas remedy is one of the Thai traditional medicines notified in the list of Herbal Medicinal Products A.D. 2021 of Thailand for the treatment of fatigue and maintaining body balance of patients. However, there have been no scientific reports on the antioxidant activities and antioxidant capacity. This study aimed to investigate antioxidant activities, and total phenolic and flavonoid contents of the three extracts of the Tri-Kasorn-Mas remedy and their mixture. The remedy plants and each ingredient were extracted by 95% ethanol maceration. These studies determined antioxidant activity by DPPH radical scavenging assay, ABTS radical cation decolorization assay, Ferric ion reducing antioxidant power assay (FRAB) and chemical contents by total phenolic and flavonoid content methods. The yield of Tri-Kasorn-Mas remedy was 26.68%. The results found that the Tri-Kasorn-Mas remedy showed strong antioxidant activity with an EC50 value of 9.37 ± 0.82 × 10–3 mg mL–1 by DPPH radical scavenging assay and 7.91 ± 0.01 × 10–3 mg mL–1 by ABTS radical cation decolorization assay. These extracts also were stronger activity than the positive control. In addition, J. multifida was the highest antioxidant activity than other ingredients. The results of this study can be preliminarily concluded that the Tri-Kasorn-Mas remedy has antioxidant activity and total phenolic compounds that are in high values.

Repurposing Hazelnut Waste Products for a Sustainable Economy: A Metabolomic Analysis of Cuticles and Shells to Highlight Their Antioxidant Potential and Inhibitory Activity against Verocytotoxic Escherichia coli

This study evaluated the functional components of hazelnut waste products (cuticles and shells) for potential use in the circular economy. HPLC-HRMS and ABTS assay were used to determine the molecules of waste products with functional properties and antioxidant capacity, respectively. The antioxidant capacity of hazelnut cuticles and shells was tested using two different methods of extraction (EtOH 50/50 and acetone 40/70) by ABTS radical cation decolorization assay. The growth inhibition effect of different extracts against porcine O138 E. coli F18+ was evaluated by the microdilution bacterial growth method and confirmed with the determination of minimal inhibitory concentration (MIC). The results showed that acetone extraction produced a higher yield of metabolites compared to ethanol extraction, and the cuticles exhibited higher antioxidant and antimicrobial potential. The acetone/water extraction led to the identification of 738 signals compared to 453 detected after EtOH/water extraction. Through metabolomic analysis, it was found that the cuticles presented a much higher quantitative and qualitative polyphenolic profile than did the shells. In addition, the ABTS assay revealed that the cuticles had a higher antioxidant capacity than did the shells. Thus, we believe that hazelnut-derived waste products show a high potential for use in a circular and sustainable economy.

Phenolic content and bioactivity of two sour cherry cultivars and their products

Bioactive compounds are produced as secondary metabolites in plants. Positive correlation between presence of bioactive compounds and health benefits of plants have been reported in many studies. Sour cherry contains high content of bioactive compounds, mostly polyphenols and anthocyanins. They are mostly consumed in fresh state, but are also used to produce jam, jelly, marmalade, juice, and syrup. Aim of this study was to evaluate total phenolic content, anthocyanins, as well as the antioxidant activity in two sour cherry cultivars and their products, jams and juice, prepared using traditional recipes. Total phenolic content was determined using Folin-Ciocalteu method and antioxidant activity was assessed using ABTS radical cation decolorization assay. pH-Differential method was used to determine anthocyanin content. Marasca cultivar had higher content of phenols, anthocyanins and antioxidant activity than Oblačinska cultivar. Processing of sour cherries had a greater impact on the reduction of anthocyanin content but did not have significant effect on antioxidant activity.

Antioxidant and Antimicrobial Activity of Algal and Cyanobacterial Extracts: An In Vitro Study.

Algae and cyanobacteria, other than their nutritional value, possess different beneficial properties, including antioxidant and antimicrobial ones. Therefore, they can be considered functional ingredients in animal feed and natural substitutes for antibiotics. The aim of this study was to evaluate the antioxidant and antimicrobial capacity against porcine O138 E. coli of Ascophyllum nodosum, Chlorella vulgaris, Lithotamnium calcareum, Schizochytrium spp. as algal species and Arthrospira platensis as cyanobacteria. The antioxidant capacity was determined by ABTS Radical Cation Decolorization Assay testing at three different concentrations (100%; 75%; 50%). The growth inhibition effect of the extracts at concentrations of 25%, 12.5%, 6%, 3% and 1.5% against porcine O138 E. coli was genetically characterized by PCR to detect the presence of major virulence factors; this was evaluated by following the microdilution bacterial growth method. The ABTS assay disclosed that Ascophyllum nodosum was the compound with the major antioxidant properties (57.75 ± 1.44 percentage of inhibition; p < 0.0001). All the extracts tested showed growth inhibition activity at a concentration of 25%. Among all extracts, A. nodosum was the most effective, showing a significant growth inhibition of E. coli; in particular, the log10 cells/mL of E. coli used as a control resulted in a significantly higher concentration of 25% and 12.5% after 4 h (8.45 ± 0.036 and 7.22 ± 0.025 log10 cells/mL, respectively; p < 0.005). This also suggests a dose-dependent relationship between the inhibitory activity and the concentration. Also, a synergistic effect was observed on antioxidant activity for the combination of Ascophyllum nodosum and Lithotamnium calcareum (p < 0.0001). Moreover, to determine if this combination could affect the viability of the IPEC-J2 cells under the normal or stress condition, the viability and membrane integrity were tested, disclosing that the combination mitigated the oxidative stress experimentally induced by increasing the cell viability. In conclusion, the results obtained highlight that the bioactive compounds of algal species are able to exert antioxidant capacity and modulate O138 E. coli growth. Also, the combination of Ascophyllum nodosum and Lithotamnium calcareum species can enhance their bioactivity, making them a promising functional feed additive and a suitable alternative to antibiotics.

The Application of Ethanolic Ultrasonication to Ameliorate the Triterpenoid Content Extracted from Vietnamese Ganoderma lucidum with the Examination by Gas Chromatography

AbstractIn this study, to investigate the extraction of triterpenoids from the Vietnamese red G. lucidum by ultrasound‐assisted extraction (UAE) method using ethanol as a solvent, the determination of the four single factors influence on the triterpenoid content including liquid‐to‐solid ratio, extraction power, extraction temperature, and extraction time was carried out for the performance of experiments on simultaneous effects by Central Composite design. The results showed that based on ultraviolet‐visible spectroscopy analysis, the highest yield of triterpenoids could be achieved with value of 18.3751 mg/g under optimal extraction conditions including extraction time of 60 min, ultrasonic power of 360 W, liquid‐to‐solid ratio of 38 : 1 mL/g, and ultrasonic temperature of 48 °C. To determine the characteristics of the obtained extract, the Gas chromatography‐mass spectrometry, the Fourier‐transform infrared spectroscopy, scanning electron microscope, and the thermogravimetric analyzer were employed in this study. Besides, for antioxidant capacity evaluation, the DPPH free radical scavenging assay, H2O2 scavenging assay, and the ABTS radical cation decolorization assay were carried out whereas the anticancer evaluation results denoted the potential for anticancer agents of G. lucidum extract. Therefore, it could be concluded that UAE is a promising method for triterpenoid extraction with antioxidant properties.

Effect of different pre-treatment maceration techniques on the content of phenolic compounds and color of Dornfelder wines elaborated in cold climate

This study present the effects of different pre-treatment maceration techniques (microwave, thermo-maceration, and enzymatic treatment) on the content of phenolic compounds (by UPLC–PDA) and their redox potential by cyclic voltammetry (CV), antioxidant capacity (ABTS radical cation decolorization assay and FRAP as Ferric Reducing Ability of Plasma), and other basic chemical properties of red wine made from cv. Dornfelder. The pre-treatment maceration technique significantly (p < 0.05) affected the content of total phenolics in Dornfelder must (freshly crushed grapes), with particularly high amounts in the must pre-treated by microwaves (4344.0 mg/100 mL), and the lowest in the must pre-treatment by thermo-maceration (2979.8 mg/100 mL). A positive correlation was found between the content of total phenolics and antioxidant activity (R = 0.69 and 0.52 by ABTS and FRAP assay, respectively) and individual groups of polyphenols (for ABTS assay with anthocyanins, flavonols, flavan-3-ols, respectively R = 0.60, 0.64 and 0.66, while for FRAP method only for anthocyanins R = 0.79). The highest antioxidant activity was also determined for the variant with microwave pre-treatment (must 3.31 mM and 3.05 mM Trolox/100 mL for ABTS and FRAP assay, respectively). Following the fermentation and maturation, the amount of polyphenols decreased. No thermal pre-treatment maceration methods gave lighter or redder must. After winemaking process all the samples were characterized by less red and more yellow shade.

Investigation of Genotoxic, Antimicrobial and Antioxidant Activities of Leaf and Flower Extracts of Cynara syriaca Boiss

The mutagenicity and antimutagenicity of leaves and flower extract of Cynara syriaca Boiss were studied with Ames assay in Salmonella typhimurium TA98 and TA100 strains. While leaves extract did not show any mutagenic effects against all the tester strains with or without metabolic activation, the flower extract showed mutagenic effect against TA98 strain without metabolic activation. On the other hand, it has been observed that the extracts have antimutagenic activity against mutations induced by sodium azide and daunomycin. The antimicrobial activity of extracts was determined by disc diffusion and MIC value. Both of the extracts possess weak antimicrobial activity. Cupric reducing antioxidant capacity (CUPRAC), DPPH free radical scavenging activity, and ABTS radical cation decolorization methods were carried out to determine the antioxidant activity. Among the tested antioxidant methods, the highest antioxidant capacity was determined in ABTS radical cation decolorization assay in which both of the extracts exhibited the best effect. Flower extract exhibited higher activity also in DPPH free radical scavenging.

Phytochemical Characterization of Five Edible Purple-Reddish Vegetables: Anthocyanins, Flavonoids, and Phenolic Acid Derivatives.

Vegetables comprise a significant portion of our daily diet with their high content in nutrients including fiber, vitamins, minerals, as well as phenolic compounds. Vegetable consumption has been shown to be positively associated with the prevention of several degenerative diseases thanks to their bioactive compounds. Accordingly, five selected vegetables, namely, red chicory, red onion, eggplant, purple sweet potato, and black carrot were thoroughly assessed for their phenolic content in this study. For this purpose, the total phenolic and flavonoid content of these five vegetables and their antioxidant activities that are based on three common methods ABTS radical cation decolorization assay (ABTS), Cupric Ion Reducing Antioxidant Capacity (CUPRAC), and DPPH scavenging activity assay were determined. Additionally, HPLC-PDA/Electrospray ionization coupled with mass spectrometry (HPLC-PDA/-ESI+-MS)-based identification and quantification of the members belonging to polyphenols present in each vegetable were determined. Statistical correlations between antioxidant activities and the specific type of phenolic compounds, such as anthocyanins, flavonoids, anthocyanins, and phenolic acids were further elucidated. Phenolic acids (chlorogenic and syringic acids) were found to be the most abundant compounds that are present in all samples. Among the anthocyanins, cyaniding derivatives were present in all vegetables. In terms of their antioxidant activities, the analyzed vegetables were ranked as red chicory > purple sweet potato > black carrot > eggplant > red onion, in descending order. Superior antioxidant activities exhibited by red chicory and purple sweet potato were attributed to the high content of phenolic compounds, especially flavonols (quercetin-3,4-O-diglucoside) in red chicory and anthocyanins (peonidin-3-caffeoyl p-hydroxybenzoylsophoroside-5-glucoside) in purple sweet potato.

ANTIOXIDANT ACTIVITY (PHENOL AND FLAVONOID CONTENT ) OF THREE DIFFERENT CULTIVARS OF PIPER BETLE L. (PIPERACEAE)

In the present study, an attempt has been made for the estimation of total phenol and flavonoid content and their radical scavenging properties using Piper betle (L.) leaves. In that, Cultivars i.e., Nov Bangla (NB), Sirugamani-1 (SGM-1) and Halisar Sanchi (HS) were selected for this study. The total phenolic content was ranged from 95.04 to 127.33 mg/100g equivalent to gallic acid and flavonoids were ranged from 51.72 to 61.08 mg/ 100g equivalent to standards of Catechin. In vitro antioxidant activity was estimated using 1,1-diphenyl-2-picryl hydrazyl (DPPH), free radical scavenging activity, improved ABTS radical cation decolorization assay and ferric reducing antioxidant power (FRAP) assay. Among all the cultivars, The highest Phenol content (93.79%) was observed for Sirugamani-1 by DPPH method and highest Phenol content (96.12% &amp; 6791.86 (µg/g) was obtained for Halisar Sanchi by ABTS assay and FRAP activity respectively. The study revealed that the leaves of Piper betle (L.) has higher amount of antioxidant activity and it could be used for any novel drug preparation.

A comprehensive LC–MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala

The current study aims to optimize and validate a comprehensive LC–MS/MS method for the quantification of 37 phytochemicals (15 phenolic acids, 17 flavonoids, 3 non-phenolic organic acids, 1 phenolic aldehyde and 1 benzopyrene) in Achillea species. Though Achillea species were chosen as real life samples, the current method is applicable to a wide range of plant species. The developed method was fully validated in terms of linearity, accuracy (recovery), inter-day and intra-day precision (repeatability), limits of detection and quantification (LOD/LOQ) and relative standard uncertainty (U% at 95% confidence level (k = 2)). Reversed-phase ultrahigh performance liquid chromatography was optimized to achive optimum separation for 37 phytochemical compounds and to overcome the suppression effects. MS detection was performed using a triple quadrupole mass spectrometer and negative or positive ionization modes were optimized for each analyte. Multiple reaction monitoring (MRM) was used to quantify the analytes, related molecular ions and transition ions were optimized. Phytochemical screening of ethanol and methanol-chloroform extracts of root and aerial parts of A. coarctata and A. monocephala were performed by using the developed and validated LC–MS/MS method. Root and aerial parts of both species have considerable amounts of certain phenolic-nonphenolic acids (quinic, malic, fumaric, chlorogenic and vanillic acids) and flavonoids (rutin, hesperidin, isoquercitrin, apigetrin, luteolin, apigenin). Additionally, total phenolic and flavonoid amounts, antioxidant (DPPH free radical scavenging assay, ABTS radical cation decolorization assay, β-carotene lipid peroxidation test system and CUPRAC cupper reduction capacity methods), anticholinesterase, tyrosinase, urease inhibition and cytotoxic activities (on HeLa (Human Cervical Carcinoma Cell Line) of A. coarctata and A. monocephala were also investigated. It has been determined that the studied Achillea species, that are rich in total phenolic-flavonoid and chlorogenic acid contents, have high antioxidant and cytotoxic potential at the same time. According to the results of LC–MS/MS, antioxidant and cytotoxic activity studies, after detailed chemical investigation and toxicity studies on these species, A. coarctata and A. monocephala may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients in future.

A comparative evaluation: phytochemical composition and antioxidant capacity of three roselle (Hibiscus sabdariffaL.) accessions

An increased consumption and demand in international trade suggests a need for quality assurance of roselle (Hibiscus sabdariffa) products. A liquid chromatography-mass spectrometry method was developed for the determination of anthocyanin profiles of the calyces, phenolic acids and flavonoids of the leaves of Jamaica, Senegal and Malaysia accessions. Delphinidin-sambubioside, delphinidin-glucoside, cyanidin-sambubioside, cyanidin-glucoside and delphinidin were identified from the calyces of these accessions. Chlorogenic acid and its isomers, glycosylated derivatives of quercetin and kaempferol, and 5-(hydroxymethyl) furfural were identified from the leaves of these accessions. No significant differences in anthocyanins among accessions were found, except the accession from Malaysia, which contained significantly (P<0.05) higher amounts of delphinidin-glucoside and delphinidin. The total phenols determined by Folin-Ciocalteu's assay were 15.92+1.12, 17.63+0.7, and 19.25+1.10 mg g-1 for the calyces of Jamaica, Malaysia and Senegal, respectively, and 23.76+2.16, 22.28+1.77 and 20.62+1.61 mg g-1 for the leaves of Malaysia, Senegal and Jamaica, respectively, calculated as gallic acid equivalents. The Senegal accession contained a higher (P<0.05) amount of total phenols in calyces than the Jamaica accession. The antioxidant activities determined by the ABTS radical cation decolorization assay were 64.38+1.56, 60.36+3.34 and 62.49+2.69 mg g-1 for the calyces and 111.43+7.17, 121.84+12.55 and 110.68+10.27 mg g-1 for the leaves of Malaysia, Senegal and Jamaica, respectively, as Trolox equivalents. Macro- and micro-nutrient elements varied significantly (P<0.05) among accessions. This study reveals variability in phenolic, antioxidant and elemental nutrient contents and suggests that the selection of roselle accessions can influence the quality of developed products.

Influence of Eu3 + doping content on antioxidant properties of Lu2O3 sol-gel derived nanoparticles

This paper presents the synthesis of pure and europium-doped lutetium oxide (Lu2O3) powders prepared by sol-gel method. The influence of europium ion concentration into Lu2O3 nanocrystallites was investigated for first time in an in vitro system using a modified ABTS radical cation decolorization assay to determine the antioxidant activity. The crystalline structure of Lu2O3 and Eu:Lu2O3 powders was elucidated by XRD obtaining cubic phase in all system without secondary products in accordance with FT-IR results. By TEM and Scherrer equation, it was determined that Lu2O3 and Eu:Lu2O3 powders presented nearly spherical particle morphology with crystallites sizes in the range of 8 to 13nm. The antioxidant assays results revealed that europium ion enhance Lu2O3 powders antioxidant properties, showing that 12.5mol% of europium is sufficient to reach its maximum capacity.

Anti-oxidant components from the aerial parts of Asterothamnus centrali-asiaticus

Two new phenolic acids 2-hydroxy-5-[(6⿲-O-(E)-caffeoyl)-β-d-glucopyranosyl]-oxybenzoic acid (1) and 2-hydroxy-5-[(3⿲-O-(E)-caffeoyl)-β-d-glucopyranosyl]-oxybenzoic acid (2) were isolated from the aerial parts of Asterothamnus centrali-asiaticus, together with five known ones (3-7). Their structures were elucidated by extensive 1D and 2D NMR studies and HRESIMS investigations. The anti-oxidant activity of the isolates was evaluated through ABTS radical cation decolorization assay. The results showed that all of them exhibited anti-oxidant activity, and compound 7 was the most active compound with an IC50 value of 7.65μg/mL.

Synthesis and Antioxidant Activity of Some New Thiazolyl–Pyrazolone Derivatives

3‐Methyl‐1‐thiocarbamoyl‐2‐pyrazolin‐5‐one has been utilized as a core for the synthesis of some 1‐(thiazol‐2‐yl)‐pyrazolin‐5‐one derivatives through diazo‐coupling reaction and/or Knoevenagel condensation followed by heterocyclization with some α‐halogenated reagents such as bromoacetone, phenacyl bromide, and ethyl bromoacetate. Base prompted addition of the core compound to an equimolar amount of phenyl isothiocyanate furnished 3‐methyl‐4‐phenylthiocarbamoyl‐1‐thiocarbamoyl‐2‐pyrazolin‐5‐one which undergoes heterocyclization with α‐halogenated reagents at the more reactive phenylthiocarbamoyl moiety to afford the corresponding 4‐(thiazol‐2‐yl)‐1‐thiocarbamoyl‐2‐pyrazolin‐5‐ones. The new synthesized thiazolyl–pyrazolone compounds were evaluated for their potential antioxidant activity by using (ABTS Radical Cation Decolorization Assay).

Evaluation of the effect of high pressure on total phenolic content, antioxidant and antimicrobial activity of citrus peels

Abstract This study aimed to assess the effect of high pressure at 300 and 500 MPa for 3 and 10 min on the phenolic compounds, antioxidant capacity and antimicrobial activity of citrus peel extracts. Total phenolic contents (TPC) and antioxidant properties of extracts were determined as free radical-scavenging ability of DPPH and using the ABTS radical cation decolorization assay. Additionally, extracts were tested for antimicrobial activity against twenty different strains of bacteria representing both Gram-positive and Gram-negative types. Citrus peel extracts demonstrated antimicrobial activity against a wide range of bacteria. The maximum level of TPC as well as antioxidant capacity were observed at 300 MPa for 3 min. Citrus peels extracts demonstrated antimicrobial activity against a wide range of microorganisms. The antimicrobial activity of orange peel extract was the highest among the four citrus peels studied. Generally, bacteria Acinetobacter and the strain Listeria innocua were more sensitive to the peel extracts.

Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves

A liquid chromatography–mass spectrometry method was developed for the simultaneous separation, and determination of natural compounds including phenolic acids and flavonoids in the leaves of Hibiscus sabdariffa. By analyzing the UV and MS data, and comparison with authenticated standards, 10 polyphenols including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin, kaempferol and their glycosides were identified together with 5-(hydroxymethyl)furfural. Major constituents in the leaves of 25 different populations from worldwide accessions were quantified and compared with each other. The total phenolic content of each accession was determined using Folin–Ciocalteu assay, ranging from 18.98±2.7 to 29.9±0.5mgGAE/g. Their in vitro antioxidant activities were measured by ABTS radical cation decolorization assay, varying from 17.5 to 152.5±18.8μmolTrolox/g. After the treatment of H. sabdariffa leaf extract, the reduction of LPS-induced NO production dose-dependently in RAW 264.7 cell indicates the extract’s potential anti-inflammatory activity.

Evaluation of in vitro antioxidant potential of Tea (Camelia sinensis) leaves obtained from different heights of Darjeeling Hill, West Bengal

Tea (Camelia sinensis) is the most consumed beverage and is also known for its medicinal value. Tea plants grown at higher altitude are subject to enhanced oxidative stress due to high UV radiation, climatic changes, and soil conditions, compared to places at low altitude. The present study was undertaken to find out whether the antioxidant activity and the content of bioactives change with the variations of altitudes in a district of Darjeeling, where tea is cultivated most. The samples were collected from six different altitudes of Darjeeling hill, viz. 6900, 5800, 4500, 3600, 2500 and 500 feet. The assays performed included ABTS radical cation decolorization assay, DPPH radical decolorization assay, reducing power assay, total polyphenols content, tannin content and total flavonoid content. It was observed that ABTS and DPPH radical scavenging abilities were reduced with increasing altitude, suggesting probable depletion of the antioxidant bioactives on exposure to extreme climatic conditions as well as elevated UV radiations. However, changes in the major bioactives of tea like tannins and flavonoids with altitude were non-significant, suggesting that although the plant tries to cope up with extreme climatic conditions, its medicinal value remains almost unchanged with altitude.

IN VITRO ANTIOXIDANT AND ALPHA-AMYLASE, ALPHA-GLUCOSIDASE INHIBITORY ACTIVITY OF FLOWERS OF CALOTROPIS GIGANTEA

The present investigations were carried out to evaluate the in vitro antioxidant as well as antidiabetic activity of flowers of Calotropis gigantea. Different extracts (petroleum ether, chloroform and ethanolic extract) were prepared using successive solvent extraction method (soxhlet) and screened for its in vitro antioxidant activity using Diphenyl picryl hydrazyl (DPPH) radical scavenging activity, ABT S radical cation decolorization assay and nitric oxide (NO) radical scavenging activity and IC50 were calculated. In vitro antidiabetic activity was studied using α – amylase and α – glucosidase inhibitory assay. Chloroform extract, among the three extracts (i.e. petroleum ether, chloroform and ethanolic), showed maximum antioxidant activity with IC50 value of 151.23µg/ml, 73.56 µg/ml and 158.92µg/ml against DPPH radical scavenging activity, ABTS radical cation decolorization assay and nitric oxide (NO) radical scavenging activity respectively. The chloroform extract produced a significant in vitro antidiabetic activity with IC50 of 52.3µg/ml 18.2µg/ml against α – amylase and α – glucosidase enzymes but less inhibitory effect than standard acarbose.

Amelioration by chlorophytum borivilianum upon arsenic induced oxidative stress in Swiss albino mice

Background: Arsenic, a major water pollutant in India, produces toxic effects on male reproductive system due to oxidative stress. Arsenic contaminated drinking water causes several health problems such as Blackfoot disease, hypertension, diabetes mellitus, disturbances in the nervous system and cancers of liver, kidney, lung and bladder in humans. Aim: This study accessed the efficacy of Chlorophytum borivilianum in reducing arsenic-induced biochemical and nucleic acid damages in mice testis. Methods: A different group of adult Swiss albino mice was made such as control group, C. borivilianum group, sodium arsenite group and combination group. For antioxidant activity, ABTS radical cation decolorization assay was done. Body and testis weight, protein, sialic acid, ATPase activity, DNA and RNA content in the testis were also estimated. Results: Animals exposed to sodium arsenite at the dose of 4.0 mg/kg b.wt. showed significant decrease (p&lt;0.001) in testicular protein, sialic acid, ATPase activity, DNA and RNA content indicate DNA damage and apoptosis whereas combination group showed a significant increase in all the parameters. Conclusion: The results thus led us to conclude that administration of C. borivilianum at the dose of 800 mg/kg b.wt. Significantly protects against arsenic-induced oxidative stress. Keywords: ABTS Assay; Atpase; DNA; Oxidative Stress; Protein; Sialic Acid.