ABTS Free Radical Research Articles

Isolation, In Vitro Antioxidant Capacity, Hypoglycemic Activity and Immunoactivity Evaluation of Polysaccharides from Coriandrum sativum L.

Coriander (Coriandrum sativum) is a classical medicinal and edible herb as well as a spice, but the physicochemical and biological properties of its polysaccharides have not been fully studied. In this study, the polysaccharides were extracted using an ultrasonic-assisted method and purified from fresh coriander, and then the coriander polysaccharide (CSP) fraction was separated using an agarose gel Q-Sepharose Fast Flow column. The total sugar content, protein content and monosaccharides composition of CSPs were determined using a phenol–sulfuric acid method, Coomassie Brilliant Blue method and HPLC. The structural characterization was detected using ultraviolet spectrophotometry and FT-IR spectroscopy. DPPH and ABTS free radicals were used to explore their antioxidant activities, while the inhibitory abilities of α-amylase and α-glucosidase were used to evaluate their hypoglycemic activity. After that, the immunomodulatory and antitumor activities were investigated using macrophage RAW264.7 and HepG2 cells as the targets. The results showed that the total sugar and protein contents of CSPs were 66.90 ± 1.44% and 1.06 ± 0.32%, respectively. CSPs were mainly composed of fucose, rhamnose, arabinose, galactose, glucose, galacturonic acid and glucuronic acid, with a molar ratio of 1.13:15.11:9.60:25.98:1.55:44.33:2.29, and may be an acidic heteropolysaccharide containing pyran rings, α- and β-glycosidic bonds and glucuronic acid. Results from in vitro experiments of biological activities showed that the IC50 of CSPs for scavenging DPPH and ABTS free radicals were 0.759 mg/mL and 1.758 mg/mL, respectively; the IC50 values for inhibiting the activities of α-amylase and α-glucosidase were 0.634 mg/mL and 2.178 mg/mL, respectively; the CSPs with a concentration of 25~200 μg/mL showed no obvious toxicity to macrophage RAW264.7, and when treated with 100 μg/mL of CSPs, the relative cell phagocytosis capacity and secreted nitric oxide amount of RAW264.7 were 153.75 ± 12.01% and 133.56 ± 5.37%, respectively; CSPs showed a concentration-dependent ability to inhibit the growth of HepG2 cells within the test concentration of 0.25–2.0 mg/mL. Summarizing the results, due to their excellent antioxidant, immunomodulatory and anti-tumor activities, the coriander acid polysaccharides were expected to show good potential in comprehensive development of food and medicine.

Enhancing Probiotic, Sensory, and Antioxidant Properties of Polygonatum sibiricum Red. and Crataegus pinnatifida Bunge through Lactic Acid Fermentation for Functional Food Development

Abstract This study explores the use of Polygonatum sibiricum Red. and Crataegus pinnatifida Bunge as edible-medicine homology ingredients through lactic acid fermentation to enhance probiotic content, sensory quality, and antioxidant properties. Using a mixed culture of Lactobacillus plantarum AS1.2437 and Lactobacillus casei CICC21019, the fermentation significantly increased lactic acid bacteria counts, improved taste, and boosted antioxidant activities, particularly in reducing Fe3+ and scavenging DPPH and ABTS free radicals (P < 0.05). During fermentation, changes in reducing sugars, total acids, superoxide dismutase (SOD) activity, and amino acid compositions were monitored. Results showed significant increases in total phenols, flavonoids, SOD activity, and polysaccharide content (P < 0.05). Essential amino acids increased by 4.86%, and therapeutic amino acids rose from 50.88% to 53.59%. The Boltzmann model effectively captured these changes, achieving a high coefficient of determination (R2 > 0.99), indicating strong predictive accuracy. Key organic acids like lactic, acetic, and tartaric acids contributed to enhanced antioxidant activity. This research provides a comprehensive understanding of the physicochemical, medicinal, and antioxidant properties of the fermented Polygonatum sibiricum Red. and C. pinnatifida Bunge (PsR.-CpB.) enzyme, establishing a theoretical foundation for developing functional foods aligned with the edible-medicine homology concept.

Study on the Extraction Technology and Antioxidant Capacity of Rhodymenia intricata Polysaccharides.

A red alga named Rhodymenia intricata was explored, and the extraction technology and antioxidant capacity of its polysaccharides were investigated. The crude polysaccharides were extracted using the ultrasound-assisted water extraction method, precipitated by alcohol, and purified using the trichloroacetic acid method. Subsequently, the scavenging rates of polysaccharides on hydroxyl, DPPH, and ABTS free radicals, were determined both prior to and following purification to evaluate their antioxidant activity. Extraction technology was optimized to improve polysaccharide yield, and the optimal parameters were as follows: particle size 100 mesh, material-liquid ratio 1:84 (g/mL), ultrasonic time 30 min, and extraction for 95 min at 80 °C. The maximized extraction rate of crude polysaccharides was 37.78 ± 0.15%. The obtained crude polysaccharides were purified with different concentrations of trichloroacetic acid, and the purification effect was evaluated according to protein removal rate and polysaccharide retention rate, which could reach 62.61 ± 1.82% and 96.10 ± 1.60%, respectively. Infrared spectrum analysis suggested that Rhodymenia intricata polysaccharide might be α-pyranose. The Congo red test illustrated that the polysaccharide contained a triple helix structure. In the antioxidant activity assessment, the scavenging rates of polysaccharide prior to purification for RIP-1 (10 mg/mL) for hydroxyl, DPPH, and ABTS free radicals were observed to achieve maximum values of 94.71 ± 0.13%, 42.80 ± 7.12%, and 76.30 ± 5.20%, respectively. In contrast, the scavenging rates of polysaccharide following purification for RIP-2 (10 mg/mL) for the same free radicals reached maximum values of 94.10 ± 0.27%, 32.37 ± 0.78%, and 98.30 ± 0.34%, respectively. Notably, these scavenging rates exhibited a dose-dependent relationship. These results demonstrated the potential of the extraction method for polysaccharides from Rhodymenia intricata, and for adding value to the by-product for its potential application as an antioxidant in food and pharmaceutical products.

Assessment of the Effect of Berberine on Metalloprotease Enzymes Inhibition and Antioxidant Activity: Possible Application in Skin Aging

Skin aging has been defined to enclose both intrinsic and extrinsic aging. Phytochemicals are frequently used for developing skin care formulations and could protect the skin’s epidermal and dermal layers, consisting mainly of elastin and collagen, from UV radiation. Berberine is an isoquinoline alkaloid and a biologically active component from plant sources. Our objective was to assess Berberine’s anti-aging capabilities by conducting elastase and collagenase enzyme inhibition and kinetic studies and to also evaluating its antioxidant capacity with three different methods. Furthermore, heat stability, pH and sun protection factor (SPF) of the formulated cream containing 1.5% berberine was evaluated. The elastase and collagenase IC50 values of berberine were estimated to be 47.54 and, 22.16 µg/mL respectively. Berberine was determined as an un-competitive inhibitor of elastase and collagenase. It scavenged DPPH and ABTS free radicals with IC50 values of 66.81 and 180.5 µg/mL respectively. 210.387 mg/L of berberine was equivalent in reducing power of 176 mg/L of ascorbic acid. SPF and pH value of cream containing berberine was found to be 12.3 and 5.62 respectively. In conclusion, these findings suggest that Berberine is a promising candidate for use as an active ingredient in cosmeceuticals, offering a natural approach to enhance skin health and reduce the visible signs of aging.

Xylooligosaccharide recovery from sugarcane bagasse using β-xylosidase-less xylanase, BsXln1, produced by Bacillus stercoris DWS1: Characterization, antioxidant potential and influence on probiotics growth under anaerobic conditions

Xylooligosaccharides (XOS) are excellent prebiotic which improve health through selective modulation of beneficial gut microbiome. Its production from agroresidues using microbial xylanase is considered as sustainable and economic approach. In this study a xylanase producing bacterium isolated from decaying wood soil was phylogenetically identified and designated as Bacillus stercoris DWS1. Xylanase (BsXln1) purified from the bacterium had pH and temperature optima of 7 and 37–60 °C, respectively, and it retained 85 % activity upon preincubation at 60 °C for 40 min. Indicating its moderate thermostability. Zymogram analysis of partially purified BsXln1 revealed its molecular weight of ∼35 kDa. B. stercoris DWS1 produced 200 U mL−1 of BsXln1 in presence of 1.5 % sugarcane bagasse (SCB) as carbon source; which was enhanced to 591 U mL−1 through optimization of cultural conditions. Xylan extracted from SCB was morphologically and structurally characterized, and then depolymerized by BsXln1 to yield XOS (400 mg g−1). Analysis of purified XOS by TLC, followed by ESI-MS showed predominance of xylobiose and xylotriose. XOS exhibited in vitro antioxidant activities against DPPH and ABTS free radicals, however, it had limited prebiotic activity on Lactobacillus plantarum and Lactobacillus fermentum under anaerobic condition. In conclusion, the xylanase, BsXln1, produced by B. stercoris DWS1 can be used in food industries for efficient production of bioactive XOS from agroresidues.

Light Intensity Effects on Productivity and Post-Harvest Quality in Perilla frutescens Cultivated in CEA

Leafy vegetables, mainly lettuces, are currently the main crop cultivated in controlled environment agriculture (CEA), including vertical farming and plant factories. There is a rising demand to expand this portfolio with a wider variety of underutilized edible plants containing various bioactive compounds and sensory properties seeking to enrich human diets. However, the optimal cultivation conditions for these underutilized plants significantly differ from those optimized for lettuce, basil, and other popular CEA crops. Therefore, this study aims to explore the impacts of light-emitting diode (LED) lighting intensity (photosynthetic photon flux density, PPFD) on green leaf Perilla frutescens cultivated in CEA. Plants were grown under four levels of LED lighting PPFDs from 150 to 300 µmol m−2s−1 for 4 weeks. Plant biomass productivity, soluble sugar contents, antioxidant properties (DPPH, ABTS free radical scavenging activities, FRAP antioxidant power), and total contents of phenolic compounds in leaves were evaluated at harvesting time. Further, harvested plant material was stored in the dark, at +6 °C, and the water content, water loss and transpiration rate, leaf sugar contents, and antioxidant properties were monitored 1, 3, and 5 days after harvesting. The summarized data suggest that higher cultivation lighting PPFD results in better harvest quality preservation during post-harvest storage.

Effects of Carbon Dots/PVA Film Combined with Radio Frequency Treatment on Storage Quality of Fried Meatballs.

The combination of carbon dots/polyvinyl alcohol (CDs/PVA) active film and radio frequency (RF) was performed to improve the storage quality of fried meatball samples. The microbicidal effect of RF, and the antioxidant and antibacterial activities of CDs/PVA film were investigated. The CDs/PVA film can effectively restrain the growth of B. subtilis, S. aureus, and E. coli, and eliminate DPPH and ABTS free radicals. RF exerts inhibitory effects on C. albicans, B. subtilis, and E. coli. For meatball samples, CDs/PVA+RF20 can extend the refrigerated shelf life from 2 w to 6 w. At the sixth week, the total bacterial count (TBC) in CDs/PVA+RF20 group (3.72 log CFU/g) was remarkably lower than those in polyethylene terephthalate (PET) group (7.78 log CFU/g) and CDs/PVA (6.41 log CFU/g) group. CDs/PVA+RF20 can also inhibit the increase in TBARS and POV values. The results manifest the feasibility of CDs/PVA+RF as a novel mild pasteurization or preservation technology.

Microcapsule Preparation and Properties of Flavonoid Extract from Immature Citrus reticulata 'Chachiensis' Peel.

Citrus reticulata 'Chachiensis' is a citrus cultivar in the Rutaceae family, and its peel is commonly utilized as a raw material for Guangchenpi. This study used flavonoid extract from the peel of immature Citrus reticulata 'Chachiensis' (CCE) as the raw material to investigate the encapsulation ability of different wall materials (plant-based proteins, including soybean protein isolation (SPI), pea protein (PP), and zein; carbohydrates, including maltodextrin (MD), Momordica charantia polysaccharide (MCP), and gum acacia (GA); and composite wall materials of both types) on CCE. The wall material with the highest encapsulation rate was selected for the preparation of CCE microcapsules. Furthermore, the physicochemical characteristics, antioxidant capacity, bioavailability, and storage stability of the CCE microcapsules were explored. The results indicated that among all wall materials, the composite wall material PPMD had the highest encapsulation rate, which was 84.44 ± 0.34%. After encapsulation, the microcapsules tended to have a yellow color and exhibited characteristics such as system stability, low moisture content, and low hygroscopicity. In vitro antioxidant assays revealed that the encapsulation of CCE significantly increased the scavenging rates of DPPH and ABTS free radicals. In vitro gastrointestinal digestion experiments indicated that the release rate of PPMD-CCE in intestinal fluid was significantly greater than that of free CCE, ultimately reaching 85.89 ± 1.53%. Storage experiments demonstrated that after 45 days under various temperature and light conditions, the retention rate of CCE in the microcapsules was significantly greater than that of free CCE. The above findings provide new possibilities for the application of PP and plant proteins and lay a foundation for the future industrial application of CCE.

Urushiol oligomer preparation and evaluations of their antibacterial, antioxidant, and thermal stability

There have been studies published on the composition and coating uses of raw lacquers following enzymatic oxidative polymerization. The change of urushiol’ thermal stability and biological activity following polymerization to create oligomer, however, has received little attention. This work using silica gel column chromatography to separate urushiol and urushiol oligomer from polymerized raw lacquer and assessed its antibacterial, antioxidant, and thermal stability in an effort to decrease the allergenicity of urushiol and increase its application. By using gel chromatography, the urushiol oligomer were discovered to be polymers with 2–5 degrees of polymerization. According to characterization results from techniques like UV, FT-IR, and 1H NMR, urushiol was converted into urushiol oligomer by addition reactions, and C–C coupling. The findings demonstrated that the urushiol oligomer’ IC50 values for scavenging DPPH and ABTS free radicals were 40.8 and 27.4 μg/mL, respectively, and that their minimum inhibitory concentrations against Staphylococcus aureus and Staphylococcus epidermidis were 250 and 125 μg/mL. The urushiol oligomer’s thermogravimetric differential curve peak temperature (461.8 °C) was higher than urushiol’s (239.5 °C), indicating that urushiol undergoes polymerization with enhanced thermal stability. The study’s findings establish a foundation for the use of polymerized urushiol and urushiol oligomer in applications including functional materials and additives.

Unravelling the Antioxidant and Antimicrobial Effect of Tecoma Stans Extracts

Infectious diseases are a serious public health problem responsible for approximately 13.7 million deaths annually. The emergence of multidrug-resistant bacterial and fungi species highlights the urgent need to search for alternative treatment options. In this regard, medicinal plants traditionally used for the treatment of infections as well as associated symptoms constitute a starting point for the search of endogenous solutions. This study was designed to investigate the antimicrobial and antioxidant properties of Tecoma stans extracts. The extracts were prepared by maceration using hydroethanolic solution (30:70, v/v) solvent. Then the obtained extract was fractioned by liquid-liquid partitioning using different solvent with increase polarity (hexane, ethylacetate, n-butanol, and water). The qualitative composition was studied using colorimetric methods. Total phenolic and total flavonoids compounds were determine using Folin Cioclateu and Aluminium Chloride methods respectively. The antioxidant activity was performed targeting DPPH, ABTS scavenging and FRAP assays. The antimicrobial activity was done using microdilution coupled with resazurin based assay. The phytochemical study of T. stans hydroethanolic extract revealed the presence of tannins, flavonoids, polyphenols, cardiac glycosides, quinones and coumarins. metabolites. The quantity of polyphenols in T. stans hydroethanolic extract was 291,139 ± 3,164 - 882,918 ± 3,154 mgEQ/100 gMS while that of flavonoids was 3,965 ± 0,138 -303,266 ± 1,178 mgEQ/100 gMS. The T. stans hydroethanolic total extract displayed no antioxidant activity targeting DPPH and ABTS free radicals at the concentrations (SC50 >500). But extracts and fractions showed a good reducing potential (SC50 ranging from 7.89 ± 0.61 tio 39.88 ± 1.10 µg/mg). Likewise, T. stans hydroethanolic extract exhibited antimicrobial activity with the MIC spanning from 1 mg/mL to 32 mg/mL toward Escherichia coli, Staphylococcus aureus, Streptococcus .........

Antioxidant Activity and Preclinical Safety of Semen persicae Extract.

Semen persicae is the dried mature seeds of Prunus persica (L.) Batsch and P. davidiana (Carr.) Franch and is commonly used in traditional Chinese medicine (TCM) formulations because of its variety of biological effects. The present study aimed to evaluate the antioxidant activity and toxicity profiles of semen persicae extract (SPE) after determining the amygdalin content (4.95%) using HPLC. Regarding the in vitro antioxidant activity, SPE with 2 mg/mL concentration scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and ABTS free radicals with rates of 51.78%, 55.47%, and 57.16%, respectively. The same concentration of SPE chelated 30.76% Fe2+. The in vitro cytotoxicity study revealed that SPE induced 92.45% cell viabilities of HEPG2 even at 2000 μg/mL. In the acute toxicity study, oral administration of SPE did not provoke mortality or any toxic signs at doses up to 2000 mg/kg bw. Repeated oral administration for 28 days at doses of 100, 300, and 600 mg/kg per day in rats did not show any toxicity signs or gross pathological abnormalities. The results of the present research provide basic reference data for SPE with a moderate effect on antioxidant activity and low toxicity for future screening of biological and pharmacological properties.

Chemical characterization and pharmacological properties of polysaccharides from Allium roseum leaves: In vitro and in vivo assays

Allium roseum is amongst the most important wild medicinal plants. It is known for its diverse biological properties, including antioxidant, antibacterial and antidiabetic activities. In this work, the polysaccharides (PARLs) were ultrasonically extracted from Allium roesum leaves then purified and analyzed by several techniques. Chemical composition and GC–MS analysis showed that the obtained polysaccharides were composed mainly of glucose (40.20 %), mannose (25.30 %), fructose (10.60 %) and galacturonic acid (15.11 %). Moreover, PARLs exhibited a potent antioxidant effect with higher capacities up to 69.61 % and 71.72 % for DPPH and ABTS free radicals, respectively. Furthermore, PARLs significantly modulated inflammatory response by reducing TNF-α, IL-6, and IL-8 pro-inflammatory mediators and promoting the anti-inflammatory IL-10 mediator in LPS stimulated THP-1 derived macrophages. The in-vivo tests proved that the extract was able to decrease carrageenan-induced rat paw swelling by around 68.15 % after 4 h of treatment. PARLs, significantly reduced the growth of U87 (glioblastoma) and IGROV-1 cancer cells with IC50 values of about 4.27 and 7.89 mg/mL respectively. This research clearly shows that Allium roseum polysaccharides can be used as natural antioxidants with anti-inflammatory and anticancer properties.

Effect of fertilizers on yield, phytochemical, and antioxidant properties of Cucurbita moschata fruits.

The nutritional and functional properties of squashes are influenced by various factors, such as the stage of plant development, soil composition, and type of fertilizer. This study evaluates the impact of various organic fertilizers on Cucurbita moschata D. properties. For this purpose, squashes were fertilized using Ash at 10 kg/25 m2, bovine compost at 62.5 kg/25 m2, and a 1:1 mixture of ash and bovine compost. Negative control (without fertilizers) and positive control (NPK 20-10-10 at 2 kg/25 m2) were included. Post-harvest, the yield, carotenoids, phenolic compounds, flavonoids, and antioxidant activities were assessed. Ash fertilizer resulted in the highest number of fruits per plant (2.20 ± 0.16). Regarding flavonoids, the bovine compost yielded the highest level (428.67 ± 2.62 mg/100 g of edible portion). The mixture of ash and bovine compost produced fruits with the highest content of total carotenoids, β-carotene, lycopene, and total phenolic compounds (249.7 ± 3.68, 219.80 ± 3.41, 26.07 ± 0.41, and 575.00 ± 9.95 mg/100 g of edible portion, respectively). Moreover, carotenoids extracted from this mixture exhibited the highest inhibition of DPPH and ABTS free radicals (30.87 ± 0.65 and 47.32 ± 1.30%, respectively). This research suggests that the use of a mixture of ash and bovine compost could significantly enhance the yield of squashes and their phytochemical and antioxidant potential.

Konjac glucomannan/microcapsule of thymol edible coating reduces okra pericarp browning by regulating antioxidant activity and ROS synthesis

Okra is susceptible to browning during storage. The effects of konjac glucomannan/microcapsule of thymol edible coating (TKL) on antioxidant activity and reactive oxygen (ROS) synthesis of okra during low-temperature storage were investigated. Thymol edible coating of thymol concentration 40 mg/mL (TKL40) had a regulatory effect on okra browning. After 14 days of storage, compared with the control group, the weight loss rate of TKL was reduced by 5.26 %, the hardness was increased by 24.14 %, and the L⁎ value was increased by 31 %. Moreover, TKL40 increased the scavenging capacity of okra for DPPH and ABTS free radicals, and activated catalase and superoxide dismutase activities by promoting the accumulation of total phenolics and flavonoids. TKL40 also reduced the cell membrane damage of okra during low-temperature storage by reducing the increase of malondialdehyde and H2O2 during okra storage. Meanwhile, it delayed the increase of relative conductivity and the production of O2.-, inhibited the activity of polyphenol oxidase in the late stage, so reduced the combination of polyphenol oxidase and phenolics to reduce the browning. Therefore, TKL40 reduces okra pericarp browning by regulating antioxidant activity and ROS synthesis.

Exploring the Antimicrobial and Antioxidant Activities of Streptomyces sp. EIZ2 Isolated from Moroccan Agricultural Soil

Antibiotics play a crucial role in preserving and improving public health, saving millions of lives every year. However, their effectiveness is currently under threat due to the ability of bacteria to adapt and develop resistance to these treatments. Therefore, this study was carried out on two soil samples collected in two areas of Arba Aounate, Sidi Bennour province, Morocco, to identify natural antibiotic-producing Actinobacteria capable of combating multi-drug-resistant (MDR) bacteria. A primary screening revealed that of the 50 isolates, 16 exhibited antimicrobial activity against Pseudomonas aeruginosa ATCC 27,853, Staphylococcus aureus ATCC 25,923, Escherichia coli ATCC 25,922, and Candida albicans ATTC 60193. A secondary screening showed that of the 16 isolates, only EIZ1 and EIZ2 isolates displayed outstanding antimicrobial and antifungal activity against 6 MDR bacteria, including Escherichia coli 19L2418, Listeria monocytogenes, Proteus sp. 19K1313, Klebsiella pneumoniae 19K 929, Proteus vulgaris 16C1737, and Klebsiella pneumoniae 20B1572. These two isolates were also characterized culturally, morphologically, physiologically, and biochemically. Afterward, the amplification of 16S rRNA revealed that isolate EIZ2 was 99.06% strongly related to the genus Streptomyces. Furthermore, this extract exhibits strong antioxidant activity against DPPH and ABTS free radicals and elevated ferric-reducing antioxidant power. A significant (p < 0.0001) positive correlation was observed between antioxidant activities and total phenolic and flavonoid contents. A GC–MS analysis of the ethyl acetate extract revealed the presence of 10 compounds, mainly diethyl phthalate (97%) and benzeneacetic acid (94%). This research demonstrates that Streptomyces sp. strain EIZ2 represents a potential source of antimicrobial and antioxidant compounds. These compounds could offer considerable potential as therapeutic agents, paving the way for future developments in medical applications.

Optimization of Fermentation Culture Medium for Sanghuangporus alpinus Using Response-Surface Methodology

The newly identified Sanghuangporus alpinus species of the Sanghuang mushroom genus has been found to possess significant medical benefits. However, the current artificial cultivation technology has not reached the requisite maturity. The response-surface methodology (RSM) was used to optimize the Sanghuangporus alpinus culture medium formulation and evaluate the functional activity of S. alpinus exopolysaccharides. First, a single-factor experiment was conducted to screen for optimal carbon and nitrogen sources for S. alpinus. Then, using Box–Behnken’s central composite design, a response-surface experiment was conducted to determine optimal culture parameters. Finally, the rationality of those parameters was assessed in a shaking flask experiment. The optimal culture parameters, determined through regression analysis, were 20.20 ± 0.17 g/L fructose (carbon source), 7.29 ± 0.10 g/L yeast extract (nitrogen source), and 0.99 ± 0.01 g/L dandelion. With optimization, the S. alpinus yield increased to 12.79 ± 1.41 g/L, twice that obtained from the initial culture medium. The S. alpinus exopolysaccharide exhibited an excellent antioxidant capacity, with the strongest scavenging effect noted on ABTS free radicals (lowest half-inhibitory concentration: 0.039 mg/mL). Additionally, this exopolysaccharide effectively inhibited various cancer cells, exhibiting the strongest activity against human glioma cells U251 (half-inhibitory concentration: 0.91 mg/mL). The RSM used to optimize the fermentation culture parameters of S. alpinus significantly increased the mycelial biomass. The improvement of Sanghuangporus alpinus yield through liquid fermentation and optimizing the fermentation medium could fill the existing gap in the cultivation of Sanghuangporus alpinus, as well as provide valuable data for the large-scale production of S. alpinus.

The metabolites of flavonoids with typical structure enhanced bioactivity through gut microbiota

The degradation of flavonoids helps to elucidate the mechanism of their biological activity, thus guiding individuals to better ingestion and utilization of these compounds. In this study, seven kinds of flavonoids with typical structures were in vitro fermented by fresh feces, and the degradation process of flavonoids were monitored by HPLC, and the gut microbiota constitutes were analyzed by 16 S rRNA microbiota sequencing. Results indicated that all seven kinds of flavonoids could be degraded by intestinal microbiota. In addition, the degradation products of flavonoids showed excellent antioxidant and anti-inflammatory activity, especially the degradation products of epicatechin at 24 h exhibited the strongest scavenging capacity for FRAP and ABTS free radicals, which were 287.494 ± 16.941 μM and 567.344 ± 13.477 μM, respectively. Moreover, compared with the corresponding control group, flavonoid treatment changed the composition of the gut microbiota, mainly by significantly reducing the abundance of harmful bacteria, such as Proteobacteria, and increasing the abundance of beneficial bacteria, such as Flavonifractor. This paper might provide theoretical basis and technical support for the nutritional and pharmacological applications of flavonoids.

Extraction, Identification, and In Vitro Anti-Inflammatory Activity of Feruloylated Oligosaccharides from Baijiu Distillers' Grains.

The structure and function of phenoyl oligosaccharides in baijiu distillers' grains (BDGs) have not been identified and investigated yet. This study aimed to elucidate the major phenolic oligosaccharides present in BDGs, optimize their extraction process via a central composite design, and assess their anti-inflammatory properties utilizing the LPS-induced RAW264.7 inflammation model. The main results are as follows: feruloylated oligosaccharides (FOs) were identified as the main phenoyl oligosaccharides in BDGs with a structure of ferulic acid esterified on arabinooligosaccharide xylose. Then, the preparation process of FOs was optimized using the following conditions: pH 5, temperature 55 °C, time 12 h, xylanase addition amount 7 g/L, BDG concentration 120 g/L. Furthermore, the acquired FOs demonstrated notable scavenging activity against DPPH and ABTS free radicals, with Trolox equivalent values of 366.8 ± 10.38 and 0.35 ± 0.01 mM Trolox/mg sample, respectively. However, their efficacy was comparatively lower than that of ferulic acid. Finally, the obtained FOs could effectively inhibit the LPS-induced secretion of TNF-α, IL-6, and IL-1β and promote the secretion of IL-10 in RAW264.7 cells. Based on the above results, FOs from BDGs were determined to have certain antioxidant and anti-inflammatory activities.

Biomimetic spray coating for fruit preservation based on UiO-1 67 metal–organic framework nanozyme

The application of edible coatings for preparing composite antibacterial spray coatings for fruit preservation by incorporating antibacterial nanoparticles has gained increasing attention. Chitosan (CS) is a natural polysaccharide used as an edible coating to preserve fruit; it has properties such as reducing water loss, enhancing appearance, and improving mechanical properties. By combining it with antibacterial material, it can reduce fruit microorganisms. Cerium (Ce) has excellent antibacterial activity combined with the advantages of safety and low cost. Therefore, this study proposes a biocatalytic spray coating for fruit preservation using a CS composite metal–organic framework (CS@Ce-MOF) with strawberry as a model fruit. CS@Ce-MOFs are superior to Ce-MOFs in the aqueous stability of their chemical structure, inoxidizability, antibacterial duration, and validity. The well-characterized CS@Ce-MOF was verified to simultaneously mimic good oxidase- and apyrase-like activities. CS@Ce-MOF biocatalytic spray coating demonstrated excellent antibacterial properties against two common foodborne pathogens: Escherichia coli and the Gram-positive bacterium Staphylococcus aureus, with high killing rates of up to 94.5%. This is due to the unique structure of the CS@Ce-MOF composite, which presents a large surface area for contact with pathogens and enhances the catalytic activity of the incorporated cerium oxide nanoparticles, leading to efficient sterilization. Furthermore, the scavenging rate of DPPH and ABTS free radicals is more than 80%, indicating that CS@Ce-MOF has excellent antioxidant properties. Moreover, CS@Ce-MOF minimized the weight loss and firmness of strawberries and bananas over 7 days of ambient storage. The use of such a biocatalytic spray coating has enormous potential for preserving the quality and safety of fresh produce, reducing food waste, and promoting sustainable agricultural practices.

Synergistic stabilization of garlic essential oil nanoemulsions by carboxymethyl chitosan/Tween 80 and application for coating preservation of chilled fresh pork

Garlic essential oil (GEO) is a potential natural antioxidant and antimicrobial agent for food preservation, but its intrinsic low water-solubility, high volatility and poor stability severely limit its application and promotion. In this work, we investigated the synergistic stabilization of the GEO-in-water nanoemulsion using carboxymethyl chitosan (CCS) and Tween 80 (TW 80). Additionally, the nanoemulsion was fabricated through high-pressure microfluidization and utilized for the coating-mediated preservation of chilled pork. The garlic essential oil nanoemulsion (GEON) with 3.0 % CCS and 3.0 % TW 80 exhibited more homogeneous droplet size (around 150 nm) and narrower size distribution, while maintained long-term stability with no significant change in size during 30 d storage. Compared with free GEO, the GEONs exhibited a higher scavenging capacity to DPPH and ABTS free radicals as well as higher inhibitory effects against Escherichia coli and Staphylococcus aureus, suggesting that the encapsulation of GEO in nanoemulsion considerably improved its antioxidant and antibacterial activities. Furthermore, the results of coating preservation experiments showed that the GEON coating effectively expanded the shelf-life of chilled fresh pork for approximately one week. Altogether, this study would guide the development of GEO-loaded nanoemulsions, and promote GEON as a promising alternative for coating preservation of chilled fresh meat.