Absorption Spectroscopy Techniques Research Articles

Nanostructured Silver Thin Film: Using Successive Ionic Layer Adsorption and Reduction Method

The Successive Ionic Layer Adsorption and Reduction (SILAR) method was utilized to produce nanostructured silver thin films on a glass substrate. Ag nanoparticles were grown using SILAR parameters such as silver nitrate (AgNO3), reduction time, reduction agent in an aqueous solution of hydrazine hydrate with various concentrations, and growth cycles. In this study, X-ray diffraction (XRD), scanning electron microscopy (SEM), and UV-visible-NIR absorption spectroscopy techniques were used to assess the produced silver thin film nanostructure. The size and spreading of the formed silver nanoparticles are observed to grow with the reducing agent and eventually saturate for constant growth cycles. As a result, the size and the distribution of the produced Ag nanoparticles grow uniformly. Higher growth cycles, however, caused Ag nanoparticles to lose their homogeneity and change into gravel-shaped particles. Therefore, silver nanoparticles that have been produced with the ideal SILAR conditions can be employed as an extremely sensitive, repeatable substrate with an exceptionally efficient factor. Moreover, the Effect of the concentration of HyH on the properties of SILAR-grown silver thin films has been extensively studied.

Possibility of lithium sulfate borate-based glass doping with Ni and Mn ions in energy storage application

This work presents the study of nickel manganese lithium sulfate borate-based glasses for energy storage application. The glasses component system, 0.2(NiO–MnO2)-0.8(xLi2S–B2O3) with x = 0.5, 1.0 and 1.5 mol, respectively, was synthesized by conventional melt-quench method. The glass structural motif was preliminarily confirmed by X-ray diffraction (XRD) technique. The oxidation states and local structural information around Ni and Mn atoms were characterized by X-ray absorption spectroscopic technique (XAS; Beamline 5.2, SLRI, Thailand). Interestingly, the oxidation states of Ni and Mn ions in all prepared vitreous samples clearly exhibited Ni2+ and Mn2+/3+. Moreover, the local bonding distances of Ni–O, Mn–O and S–O were also revealed by the extended x-ray absorption fine structure (EXAFS) measurement. In order to study a possibility of prepared glasses in energy storage application, the glass cathodes were successfully prepared and investigated using an electrochemical study with CV measurement.

Combining XAS with XRD for elucidating the complex structural behavior of pre-lithiated SnO anode materials for Lithium-ion batteries

Tin-based oxides anode materials have been widely studied to aim at higher performance for next-generation lithium-ion batteries (LIBs) due to the high theoretical specific capacity of Sn (reaching 994 mAh.g−1). However, the volume variation of Sn/LixSn transformation changes during the lithiation prevents a common application of these materials in nowadays used batteries. To understand the lithiation pathways, in this work, the degree of pre-lithiation of tin-base anode materials was investigated via combined X-ray diffraction (XRD) and X-ray absorption spectroscopy (XAS) techniques. The experimental results confirm the formation of LiSn, Li7Sn3, Li5Sn2, Li13Sn5, Li7Sn2, and Li22Sn5 phases. The degree of pre-lithiation plays a critical role in determining the electrochemical characteristics of LixSn alloy. This work provided useful and new insights in the future development of advanced anode materials for LIBs.

Room-temperature synthesis of CsPbBr3@CsPb2Br5 microcrystals and their upconversion luminescence

In this work, a convenient and environmental-friendly aqueous-phase method is used to synthesize CsPbBr3@CsPb2Br5 microcrystals (MCs) at room temperature, of which structural and optical properties are characterized by powder X-ray diffraction (PXRD), absorption, and photoluminescence (PL) spectroscopic techniques. Two obvious absorption peaks at 307 and 515 nm are attributed to CsPb2Br5 matrix and CsPbBr3 impurity, respectively. Full-width at half-maximum (FWHM) of PL for MCs is 83.5 meV at room temperature, and its emission peak is observed at 518 nm. With the temperature changed from 80 to 340 K, the fitted value of the exciton binding energy is 84.1 meV from temperature-dependent steady-state PL. Upconversion luminescence (UCL) can be observed obviously under near-infrared femtosecond laser pulse excitation, which is compared with normal luminescence from steady-state and time-resolved PL measurements. The experimental investigations show that CsPbBr3@CsPb2Br5 MCs have excellent stability against thermal, water and light irradiation, which will provide a new choice for the commercial applications to lasering and displaying.

Intercomparison of NO3 under Humid Conditions with Open-Path and Extractive IBBCEAS in an Atmospheric Reaction Chamber

We report an open-path incoherent broadband cavity-enhanced absorption spectroscopy (OP-IBBCEAS) technique for in situ simultaneous optical monitoring of NO2, NO3, and H2O in a reaction chamber. The measurement precision values (1σ) are 2.9 ppbv and 2.9 pptv for NO2 and NO3 in 2 s, respectively, and the measurement uncertainties are 6% for NO2 and 14% for NO3. Intercomparison of measured concentrations of NO2 and NO3 by open-path and extractive IBBCEAS was carried out in the SAES-ARC reaction chamber during the reaction of NO2 with O3. The measurement accuracy of OP-IBBCEAS is verified by an NO2 intercomparison and the NO3 transmission efficiency of the extractive IBBCEAS is determined by comparison against the in situ NO3 measurement. The relationship between H2O absorption cross section and its mixing ratio at 295 K and 1 atm was analysed. Due to the spectral resolution of IBBCEAS system, the strong and narrow absorption lines of H2O are unresolved and exhibit non-Beer–Lambert Law behaviour. Therefore, a correction method is used to obtain the effective absorption cross section for fitting the H2O structure. An inappropriate H2O absorption cross section can cause an overestimation of NO3 concentration of about 28% in a humid atmosphere (H2O = 1.8%). This spectroscopic correction provides an approach to obtain accurate NO3 concentrations for open-path optical configurations, for example in chamber experiments or field campaigns. The measurement precision values are improved by a factor of 3 to 4 after applying Kalam filtering, achieving sub-ppbv (0.8 ppbv) and sub-pptv (0.9 pptv) performance in 2 s for NO2 and NO3, respectively.

Ion Exchange Properties of Crosslinked and Carboxylated Peanut (Arachis Hypogaea L.) Testa Extract

After polymerization via polycondensation of catechin units in the acetone extract of peanut (arachis hypogaea L.) testa (PTE) using phosphorus oxychloride (POCl3) as a crosslinking agent, POCl3-crosslinked PTE (Crosslinked-PPTE) was functionalized by etherification (PC-PTE) using monochloroacetic acid (MCAA) in the presence of an alkali according to the method of Williamson ether synthesis. Subsequent to characterization by Fourier Transform Infrared spectroscopy (FTIR) as well as physical properties, Crosslinked-PPTE was assessed for cation exchange capacity (CEC) and pH at point of zero charge (ZPHC) following demonstrated capacity to remove heavy metal ions in single and multi-elements aqueous solutions by Atomic Absorption Spectrometry (AAS) technique while functionalized (carboxymethylated) Crosslinked-PPTE (PC-PTE) was further characterized by degree of substitution (DS). FTIR absorption bands due to -C-O-P=O, -P=O, P-O-C- and R-O-P-OH stretching vibrations were seen at 1350/1250, 1244/1246, 997/993 cm-1 respectively. Asymmetric and symmetric stretching vibrations of the -C=O of a COOH, Ar-O-C- of an aromatic ether as well as -P-O-C - of an aromatic PO43-, R-P=O of a -PO43-, -R-O-P- and R-O-P-OH of a PO43- ester created by crosslinked and carboxymethylated PTE respectively were seen at 1743, 1377, 1278, 1190, 1062 and 823 cm-1. The results obtained showed that 0.6 was the DS of PC-PTE while 6.72 meq/g and 3.57 defined the CEC and pH (zpc) respectively of Crosslinked-PPTE. Quantitative removal of Fe2+, Ni2+, Pb2+, Cu2+ and Zn2+ in laboratory simulated metal ion wastewater (LSW) to the levels of 84.4, 81.7, 76.5, 72.0 and 75.1% respectively was achieved using Crosslinked-PPTE resin in batch adsorption experiments in which the efficiency was found to depend significantly on the temperature and pH of the water as well as initial adsorbate concentration and contact time but partially on the amount of adsorbent used was observed to be independent of the speed of stirring of the wastewater. Stable, Matakana and fine powder crosslinked-PPTE which is insoluble in water and common laboratory solvents holds great potential in the purification of heavy metal ions polluted water

Development and validation of Graphite Furnace Atomic Absorption Spectrometry method and its application for clinical evaluation of blood lead levels among occupationally exposed lead smelting plant workers.

The growing interest in estimating the blood lead levels, for early detection of lead exposure, warranted a need for a validated analytical method for trace levels estimation of lead. The present study aimed to develop an analytical method for detecting trace amounts to elevated levels of lead in human blood using the Graphite Furnace Atomic Absorption Spectrometry technique and its application in evaluating blood lead levels among occupationally exposed individuals. The method validation was performed with standard test parameters including linearity, recovery, precision, method detection limit, and limit of quantification. The validation results for each performance parameter were in agreement with acceptable criteria as per standard guidelines. The correlation was observed as optimum linear (R2 = 0.998) between absorbance and lead concentration range from 0 to 10µg/dL. The recoveries for spiked samples ranged between 95 and 105%. The calculated value for the method detection limit was 0.16µg/dL and the limit of quantification was 0.51µg/dL. The precision for all spiked concentrations was below 10% of the relative standard deviation. Evaluation of lead exposure among occupationally exposed individuals revealed the study population had found average blood lead level (42.80 ± 12.47µg/dL), which was above the upper acceptable limit suggested by Occupational Safety and Health Administration, USA. The majority of system-specific symptoms were observed among study groups having mean blood lead levels above 40µg/dL. However, sociodemographic status and employment factors were found possible determinants of the prevalence of high blood lead levels.

Comparison of Digestion Methods Using Atomic Absorption Spectrometry for the Determination of Metal Levels in Plants

Trace metal elements (TMEs) are among the most important types of pollutants in the environment. Therefore, a precise determination of these contaminants in several environmental components is required for the safety assurance of living organisms. Spectroscopic analysis is an efficient technique employed to detect and determine TME contents in numerous samples. Hence, to achieve reliable and accurate results when using spectroscopic analysis, samples should be carefully prepared. In the present study, the comparison of eight digestion methods of five vegetal samples was carried out to quantify Cd, Mn, Al and Mg contents using the atomic absorption spectroscopy technique. According to the extraction techniques used in this study, results showed an outstanding difference in TME levels determined in the same vegetal sample. The results obtained indicated that the highest Mn concentrations were recorded when using the mixture of HNO3-HClO4 in the studied species: atriplex portulacoides, arthrocnemum indicum, olea europaea BCR-62, ulva lactuca and ulva lactuca BCR-279 compared to all other methods. Regarding the extraction of Cd, our results showed that heated extraction using different acids (HNO3-H2SO4-HClO4, HCl-HNO3, HNO3-HClO4, HNO3-H2SO4, HNO3-HCl-HClO4 and HNO3-HCl-H2SO4) was the most efficient in atriplex portulacoides, arthrocnemum indicum, olea europaea BCR-62, ulva lactuca and ulva lactuca BCR-279. Similarly, these heated acid digestion techniques (efficient for Cd) showed the highest levels of Al in atriplex portulacoides and arthrocnemum indicum. However, for the Mg extraction, our results revealed that the effectiveness of the method used depended on the plant species studied. Regarding these findings, the efficiency of metal quantification by AAS depends on the digestion procedure, the metallic ion to determine and the plant species.

Performance of micro-beam X-ray absorption spectroscopy at beamline 7.2W of Synchrotron Light Research Institute

The existing BL7.2W beamline at SLRI, initially developed for Macromolecular Crystallography, was upgraded with a Si-drift X-ray detector, sample micro-stage, and polycapillary X-ray lens to provide possibilities for experiments using X-ray fluorescence (XRF) imaging and micro-beam X-ray absorption spectroscopy (μXAS) techniques. The micro X-ray beam was characterized. The beam diameter of approximately 25 μm was determined from the knife-edge scan method. The measured photon flux was 3.8 × 109, 1.8 × 109, and 6.4 × 108 photons/s for 8.0, 10.0, and 12.0 keV X-rays, respectively. Imaging XRF and μXAS measurements were successfully performed on a welding joint of oxygen-free high thermal conductivity copper and AISI304 stainless steel. This opens opportunities for SLRI users to utilize this new measurement setup for elemental and chemical characterizations of small areas on various samples.

Lidar sounding of carbon dioxide and water vapor with absorption spectroscopy techniques

A possibility of monitoring greenhouse gases (CO2 and H2O) along horizontal paths in the troposphere with two-channel near-IR lidar system based on an absorption spectroscopy measurement technique is estimated. The main units and components of the IR lidar under development are described. A backscattered signal is simulated in the informative range of IR lidar for sensing target greenhouse gases.

Effect of substrate on interfacial electronic properties of ferrocene thin films

Ferrocene molecular thin films on conducting substrates have potential for novel molecular electronic devices. Based on X-ray absorption spectroscopy and photoemission spectroscopic techniques, we have analyzed electronic properties of ferrocene thin films nano-junctions on layered highly oriented pyrolytic graphite (HOPG) and epitaxially grown graphene on Ni(1 1 1) substrates. Fe L edge analysis indicates that layered structure of HOPG has significant impact on room temperature desorption of ferrocene as compared to grapheme on Ni(1 1 1) substrate. C-K edge XAS spectrum reveal that ferrocene molecules do not have preferred orientation for adsorption on grapheme grown on Ni(1 1 1) substrate. In addition, X-ray absorption spectroscopy (XAS) reveals that there is no change in iron atom oxidation state suggesting that ferroence interaction is feeble with grapheme on Ni(1 1 1) substrate and HOPG.

Retrieval and Regional Distribution Analysis of Ammonia, Sulfur Dioxide and Nitrogen Dioxide in the Urban Environment Using Ultraviolet DOAS Algorithm

Aiming at the in situ and mobile observation of urban environmental air pollution, a portable instrument using ultraviolet spectrum retrieval algorithm was developed based on the basis of Differential Optical Absorption Spectroscopy (DOAS) and multiple-pass cell technique. Typical trace gas pollutants, NH3, SO2, and NO2, were explored using their optical spectral characteristics in deep ultraviolet wavelength range from 210 to 215 nm. The gas concentration was retrieved by Lambert-Beer's law and nonlinear least square method. With an optimized optical alignment, the detection limits of NH3, SO2, NO2 were estimated to be 2.2, 2.3, and 36.2 ppb, respectively. The system was used in carrying out some cruise observations in Chengdu, China. During the entire period, the polluted gases showed varied distribution and typical daily average concentrations of NH3, SO2, NO2 were 23.2, 3.5, and 106.0 ppb, respectively. The contributions from different sources were analyzed combined with the HYSPLIT model. Results show that the portable DOAS system is a convenient and effective tool for regional distribution measurement and pollution source monitoring.

In situ grown high-valence Mo-doped NiCo Prussian blue analogue for enhanced urea electrooxidation.

Mo-doped NiCo Prussian blue analogue (PBA) electrocatalysts self-supported on Ni foam are elaborately designed, which exhibit a low potential of 1.358 V (vs. RHE) to reach 100 mA cm-2 for catalyzing the urea oxidation reaction (UOR). The incorporation of high-valence Mo (+6) modifies the electronic structure and improves the electron transfer ability. Using X-ray photoelectron spectroscopy (XPS) and X-ray absorption spectroscopy (XAS) techniques, we confirm the effect of Mo doping on the NiCo PBA electronic structure.

A near infrared H2S leakage detection system based on WDO-ELM using a digital lock-in amplifier combined with discrete wavelet transform filter

A gas leakage detection system was developed based on tunable diode laser absorption spectroscopy (TDLAS) technique with the combination frequency absorption band of hydrogen sulfide (H2S) gas molecules at 1578 nm for telemetry. For further telemetry distance and signal-to-noise ratio (SNR), a Fresnel lens with a diameter of 250 mm is utilized to focus the laser beam that diverges gradually with the increase of telemetry distance. A digital lock-in amplifier (DLIA) combined with discrete wavelet transform (DWT) filter was devised to process faint photoelectric signal received by InGaAs photodiode. Meanwhile, a global optimization algorithm, combined wind driven optimization (WDO) with extreme learning machine (ELM), named WDO-ELM algorithm, is applied for H2S concentration inversion to make up the limitation of ELM algorithm and seek for the optimal weight and threshold in a more extensive range. The reliability and sensitivity of the H2S leakage detection system have been verified by calibration and stability experiments. The limit of detection (LoD) reaches a minimum of 0.983 parts-per-million·meter (ppm·m) with a 96 s integration time by Allan-Werle deviation analysis. This self-developed H2S leakage detection system with high integration and low cost is more convenient for commercial application in tank farm to realize remote detection of gas leakage.

Beta decay along the N=Z line and its relevance in rp-process and X-Ray bursts

Nucleosynthesis in Type I X-ray bursts (XRB) proceeds eventually through the rp-process near the proton drip-line. Several N=Z nuclei act as waiting points in the reaction network chain. Astrophysical calculations of XRB light curves depend upon the theoretical modelling of the beta decays of interest, with the N=Z waiting points 64Ge, 68Se, 72Kr, 76Sr, and their second-neighbours N=Z+2 being key nuclei in this context. We have carried out different experimental campaigns at ISOLDE (CERN) to determine the B(GT) distributions, in the decay of several N=Z, N=Z+2 and their daughters, of particular relevance in rp-process calculations. To this aim the Total Absorption Spectroscopy technique is applied. Here we present results on the beta decay of 64Ga and the status of the analysis of 64Ge. Our results provide benchmarks for testing and constraining models under terrestrial conditions that can be used later for predictions in stellar environments.

Optimization of beam arrangement for tunable diode laser absorption tomography reconstruction based on fractional Tikhonov regularization

Beam arrangement with limited projections based on tunable diode laser absorption spectroscopy is the key to achieving a more accurate two-dimensional reconstruction of the combustion. Using fractional calculus theory, a beam optimization method based on fractional Tikhonov regularization is proposed. The beam arrangement function based on fractional Tikhonov regularization is established by extending the standard Tikhonov regularization to fractional modes. The genetic algorithm is used to analyze the calculation results of different orders in a range of (0, 1), and the beam arrangement is obtained. Using 20 laser beams to scan the characteristic absorption spectrum of H<sub>2</sub>O in the near-infrared band 7185.6 cm<sup>–1</sup>, modeling the calculations in a 10×10 element discrete tomography domain, and comparing the reconstruction results of the five beam arrangements for different Gaussian distribution models, the beam arrangement based on fractional Tikhonov regularization shows more obvious advantages. This design method proposed in this work is valuable for the theoretical study of the optimal design of two-dimensional measurement beams based on the tunable diode laser absorption spectroscopy technique, which can promote the application of this technique in the two-dimensional reconstruction of complex engine combustion and combustion efficiency improvement.

Noise Immune Absorption Profile Extraction for the TDLAS Thermometry Sensor by Using an FMCW Interferometer

Tunable diode laser absorption spectroscopy techniques usually utilize direct absorption spectroscopy (DAS) to measure absorbance profiles for temperature measurements. The DAS method is sensitive to thermal noises and fails to work in harsh application cases. A noise immune method is proposed for robust temperature measurement by using coherent detection and retroflected optical alignment. The absorption spectrum is shifted to a high-frequency band of MHz level away from ambient noises. The optical alignment suppresses thermal radiations along the laser path. Gas temperatures were measured in cases of both laboratory and porotype setup of aeroengine, i.e., the steady and the harsh states. Transition lines of H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O at 7182.94 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> , 7185.59 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> , and 7444.35 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> were selected for performance evaluations of target molecules, i.e., of water vapor. Performance comparisons were made with the DAS method for temperature detection. In a steady-state case, standard deviations of the proposed method were below 0.9 K at SNR of 25 dB, while those of the DAS method were above 3.9 K. In an onsite harsh application case, the fluctuations of path-averaged temperatures obtained by the proposed method were also smaller. The coherent detection and retroflected alignment enable better performance in hostile cases.

High precision measurement of spectroscopic parameters of H&lt;sub&gt;2&lt;/sub&gt;S in 6320—6350 cm&lt;sup&gt;–1&lt;/sup&gt; band

As a highly corrosive and highly toxic gas, hydrogen sulfide (H&lt;sub&gt;2&lt;/sub&gt;S) is an important intermediate product or pollutant in many fields such as chemical industry, energy and environment. Accurate online measurement of its concentration is of great significance for process control and production safety. Tunable diode laser absorption spectroscopy (TDLAS), as a quantitative absorption spectroscopy technique, is suitable for high-precision on-line measurement of H&lt;sub&gt;2&lt;/sub&gt;S concentration in atmospheric environmental monitoring and industrial processes control. Considering that most of the spectroscopic parameters of H&lt;sub&gt;2&lt;/sub&gt;S in the HITRAN2020 database are mainly calculated based on semi-empirical theoretical model and the experimental data to support them are lacking. In this work, direct absorption spectroscopy (DAS) method is firstly used to measure the absorption spectra of H&lt;sub&gt;2&lt;/sub&gt;S in the band of 6320–6350 cm&lt;sup&gt;–1&lt;/sup&gt;. Six groups of characteristic lines with strong absorption and relative independence are selected as the target transitions for experimental measurement. Then, the wavelength modulation-direct absorption (WM-DAS) method with no calibration and high signal-to-noise ratio is used to measure the absorbances of the six groups of transitions under different pressures. Voigt, Raution and quadratic speed-dependent Voigt profiles fit the measured absorbances by least squares method in order to obtain the spectroscopic parameters such as the collision broadening coefficient, line strength and Dicke narrowing coefficient. And the minimum standard deviation of residual error of absorbances is 7×10&lt;sup&gt;–5&lt;/sup&gt;. The measurement uncertainty of each line strength is less than 2%, and the uncertainty of collision broadening coefficients, Dicke narrowing coefficients and the speed-dependent coefficients are all less than 10%. This work is helpful in improving the H&lt;sub&gt;2&lt;/sub&gt;S spectral database and providing the spectral data basis for the high-precision measurement of H&lt;sub&gt;2&lt;/sub&gt;S concentration.

Determination of Heavy Metals in Cocoa Products Consumed in Nairobi County Kenya

Cocoa is an important cash crop obtained from the beans of the Theobroma cacao tree belonging to the family Malvaceae. Its products are rich in several classes of phenolic compounds such as Flavanols, procyanidins, and anthocyanins. These compounds are attributed to copious health benefits. In addition to the bioactive compounds, cocoa contains heavy metals whose origin and levels depend on natural and anthropogenic activities. Beyond permissible limits, these metals are linked to deleterious damage to several systems in the human body. This study aims to determine the concentration of some heavy metals in Cocoa products and compare the levels with the permissible exposure limits stipulated by international health agencies. This was achieved by wet digestion of the strategically sampled cocoa products and their analysis using the atomic absorption spectrometry technique. The average concentration of Lead (Pb), Copper (Cu), Zinc (Zn), and Manganese (Mn) in cocoa powder was 0.154±0.021, 0.270±0.037, 0.794±0.063, and 0.334±0.015 respectively. The average concentration of Pb, Cu, Zn, and Mn in cocoa butter was 0.329±0.008, 0.028±0.038, 0.116±0.075, and 0.124±0.008 respectively. The average concentration of Pb, Cu, Zn, and Mn in chocolate milk was 0.463±0.003, 0.042±0.066, 0.296±0.015, and 0.162±0.010 respectively. Cadmium levels were below the detection limit in all the samples. The results obtained in all the samples were within the WHO and KEBS permissible levels in food products.

A Green Product Using Selective Compound for Susceptible Assessment of Copper in Blood Serum

A highly reliable approach for assessment of copper(II) in blood serum samples using an organic molecule 4-(3-hydroxyphenylazo)resorcinol, 4-HAR was developed. The paper presents the synthesis of 4-HAR, which serves as probe for definite species. 4-HAR was characterized with the FT-IR, UV-visible, 1H-NMR, 13C-NMR and mass analysis, thermal TG and DSC techniques. After examining its interactions with more than twenty-five metal ions, it was found that 4-HAR showed reaction only with copper(II), giving a distinct green color at λmax 603 nm. Experimental conditions for the reaction of 4-HAR with copper(II) were optimized. A calibration curve was created and the exhibition range of concentration obeyed Beer's Law from 0.30 to 16 μg.mL-1 with correlation coefficient of r = 0.9986, and value of the molar absorption coefficient, ε, being 3314 L.mol- 1.cm-1. The analytical method was used to determine copper(II) levels in several blood serum samples. The amounts of copper(II) found in the sera samples were compared with flame atomic absorption spectrometry technique, and the results were found to be highly reliable. Based on validation, the new approach can be utilized for assurance quality purposes with a high degree of confidence.