Absolute Minimum Research Articles

Relationship between rain and groundwater in the hydrogeological sectors of the South Basin of Ciego de Ávila

Introduction: Groundwater constitutes the main source of water in the province of Ciego de Ávila, Cuba; especially in agriculture, which occupies the main economic line, however the overexploitation of aquifers in the southern basin of the province puts the sustainability of this important resource at risk, which is renewed through the natural recharge of rainfall. Objective: To determine the relationship between precipitation and groundwater levels in the hydrogeological sectors CA-II-2 and CA-II-3 of the Ciego de Ávila South Basin, which can constitute a way for the efficient use of water for irrigation purposes in this area of ​​great agricultural importance. Methodology: In the development of the research, different methodologies were used: selection of the most representative rain gauges, calculation of effective precipitation, determination of accumulated monthly and annual rainfall; as well as the annual rainfall of the wet and dry periods, completion of rainfall series by the linear regression method, use of the HidroEsta 2 program to obtain the mean hiperanual rainfall and the historical mean levels, absolute minimum and maximum of all the selected wells. The mean rainfall of the area of ​​the sectors was determined with the Isohyet method and the charts of hiperanual limnigrams were constructed using the BARHIS software. Results: The investigation showed that the average rainfall in the area is 1303.6 mm, higher than the historical average for the province. The water levels in the aquifer range between 0.84 and 12.98 m; where 7.21 m is the absolute average value. The year 1988 had great variations in rainfall, levels and the exploitation of groundwater. This study suggests the need to take measures that contribute to the rational use of groundwater in this overexploited basin due to the intensive use of water resources in agricultural irrigation. Conclusions: The study carried out was able to verify that the average rainfall in the area is 1303.6 mm, higher than the historical average for the province. The water levels in the aquifer range between 0.84 and 12.98 m, with absolute averages of 6.52 and 7.21 m respectively. The year 1988 presented high variability in rainfall, underground levels and the exploitation of groundwater.

Redesigning and teaching veterinary microbiology laboratory exercises with combined on-site and online participation during the COVID-19 pandemic.

ABSTRACTThe COVID-19 (coronavirus disease 2019) pandemic has forced universities to find new ways to conduct learning and teaching, as traditional face-to-face teaching has been prevented or restricted to an absolute minimum in many instances. Therefore, we redesigned and taught second-year veterinary student microbiology laboratory exercises (labs) with a hybrid learning approach. For this, a novel ‘remote partner’ model was implemented in which students present on-site in the laboratory worked synchronously pairwise with their remote partner present online. A student feedback survey revealed that in this remote partner model, both on-site and online participation in the labs were experienced as being useful in improving their laboratory skills. The students' overall performance in hands-on microbiological laboratory skills and safe working practices was similar in the hybrid learning approach (the 2021 class) and in the traditional on-site participation approach (the 2018–20 classes). This study suggests that the remote partner model is an effective way to acquire microbiological laboratory skills. This learning approach can be used in the non-pandemic future and/or also be applied to other fields.

Is Solar Minimum 24/25 Another Unusual One?

Abstract Solar minimum 23/24 is considered to be unusual because it exhibits features that differ notably from those commonly seen in previous minima. In this Letter, we analyze the solar polar magnetic field, the potential-field solution of the solar corona, and the in situ solar wind measurements to see if the recent solar minimum 24/25 is another unusual one. While the dipolar configuration that is commonly seen during minimum 22/23 and earlier minima persist for about half a year after the absolute minimum of solar cycle 24, the corona has a morphology that is more complex than a simple dipole before the absolute minimum. The fast solar wind streams are less dominant than minimum 23/24. The interplanetary magnetic field strength, density, and mass flux that are historically low in the minimum 23/24 are regained during minimum 24/25, but still do not reach the minimum 22/23 level. From the analysis of this Letter, it seems that the minimum 24/25 is only partly unusual, and the recovery of the commonly minimum features may result from the enhancement of the polar field.

Spatiotemporal Analysis of the Frost Regime in the Iberian Peninsula in the Context of Climate Change (1975–2018)

Climate change is having many effects in the agricultural sector, which are being studied worldwide. Undoubtedly, warmer winters and earlier springs produce changes in frost regimes and severity that will affect the sustainability of agricultural production in the area. The Mediterranean region and the Iberian Peninsula (IP) are among the areas where the greatest impact of climate change is expected. Daily data from 68 weather stations of the IP belonging to the European Climate Assessment and Dataset (1975–2018) were used to conduct a spatiotemporal study of the frost regime. The variables calculated include the probability of three frost types according to their severity, frost day, mean absolute minimum yearly temperature, first frost day, last frost day, and frost-free period. These variables were integrated into a geographic information system, which allowed the graphical visualization of their patterns using of geostatistical interpolation techniques (kriging). Changes in frost variables were investigated using the Mann–Kendall test and Sen’s slope estimator. A general reduction in the number of frosts per year is observed (values between −0.04- and −0.8-day frosts per year), as well as an increase in the mean absolute minimum temperature (values between 0.04 and 0.10 °C per year), with very high significant trends throughout the territory. The reduction in the number of frosts is more pronounced at a higher elevation. Frost dates vary greatly due to the orographic characteristics of the IP. The generalized trend is of a significant delay of the autumn frosts (values between 0.4 and 1.06 days/year), as well as early spring frosts (between −0.429 and −1.29 days/year), and as a consequence a longer frost-free period, all changes were much stronger than those found in other regions of the world. These effects of climate change must be mitigated by modifying species, varieties, and cultivation techniques to guarantee sustainable agriculture.

Differentiation of Pulmonary Lymphoma Manifestations and Nonlymphoma Infiltrates in Possible Invasive Fungal Disease Using Fast T1-weighted Magnetic Resonance Imaging at 3 T Comparison of Texture Analysis, Mapping, and Signal Intensity Quotients.

This study aimed to evaluate the diagnostic performance of texture analysis (TA), T1 mapping, and signal intensity quotients derived from fast T1-weighted gradient echo (T1w GRE) sequences for differentiating pulmonary lymphoma manifestations and nonlymphoma infiltrates in possible invasive fungal disease in immunocompromised hematological patients. Twenty patients with hematologic malignancies and concomitant immunosuppression (including 10 patients with pulmonary lymphoma manifestations and 10 patients with nonlymphoma infiltrates) prospectively underwent 3 T magnetic resonance imaging using a conventional T1w GRE sequence and a T1w GRE mapping sequence with variable flip angle. A region of interest was placed around the most representative lesion in each patient. TA was performed using PyRadiomics. T1 relaxation times were extracted from precompiled maps and calculated manually. Signal intensity quotients (lesion/muscle) were calculated from conventional T1w GRE sequences. Of all TA features, variance, mean absolute deviation, robust mean absolute deviation, interquartile range, and minimum were significantly different between the 2 entities (P<0.05), with excellent diagnostic performance in receiver operating characteristic analysis (area under the curve [AUC] >80%). Neither T1 relaxation times from precompiled maps (AUC=63%; P=0.353) nor manual calculation (AUC=63%; P=0.353) nor signal intensity quotients (AUC=70%; P=0.143) yielded significant differences. TA from fast T1w GRE images can differentiate pulmonary lymphoma manifestations and nonlymphoma infiltrates in possible invasive fungal disease with excellent diagnostic performance using the TA features variance, mean absolute deviation, robust mean absolute deviation, interquartile range, and minimum. Combining a fast T1w GRE sequence with TA seems to be a promising tool to differentiate these 2 entities noninvasively.

Nonstationary analysis of the extreme temperatures in Turkey

Record-breaking extreme temperatures have been measured in the last two decades all over Turkey, with recent studies detecting positive trends in extreme temperature time series. In this study, nonstationary extreme value analysis was performed on extreme temperature time series obtained from fifty stations scattered over the seven geographical regions of Turkey. Basic characterization of the data set was defined through outlier detection, homogeneity, trend detection, and stationarity tests. Trend-including non-stationary extreme temperature time series were analyzed with non-stationary Generalized Extreme Value distribution. Three main physical drivers were considered as the leading causes that trigger the observed trends in extreme temperatures over Turkey: time, teleconnection patterns of the Arctic Oscillations, and those of the North Atlantic Oscillations. The results showed that most of the absolute annual minimum and maximum temperature time series are inhomogeneous while the possible breakpoints date back to the1970s and 1990s, respectively. More than half of the absolute annual maximum time series (26/50 and many of the absolute annual minimum time series (21/50) showed a positive trend. No negative trend was detected in the extreme temperature time series. Based on the frequency analysis of the 21 annual maximum time series, the non-stationary estimations of 50-year return levels were detected to be higher than in the stationary model (between 0.44 °C and 3.73 °C). The return levels in 15 of the 20 minimum temperature time series increased from 0.11 °C up to 12.28 °C. Elevation increases the nonstationarity impact on absolute minimum temperatures and decreases it on absolute maximums. The findings in this study indicate that the consideration of non-stationarity in extreme temperature time series is a necessity during return level estimations over the study area.

Future trends in measuring physiology in free-living animals.

Thus far, ecophysiology research has predominantly been conducted within controlled laboratory-based environments, owing to a mismatch between the recording technologies available for physiological monitoring in wild animals and the suite of behaviours and environments they need to withstand, without unduly affecting subjects. While it is possible to record some physiological variables for free-living animals using animal-attached logging devices, including inertial-measurement, heart-rate and temperature loggers, the field is still in its infancy. In this opinion piece, we review the most important future research directions for advancing the field of 'physiologging' in wild animals, including the technological development that we anticipate will be required, and the fiscal and ethical challenges that must be overcome. Non-invasive, multi-sensor miniature devices are ubiquitous in the world of human health and fitness monitoring, creating invaluable opportunities for animal and human physiologging to drive synergistic advances. We argue that by capitalizing on the research efforts and advancements made in the development of human wearables, it will be possible to design the non-invasive loggers needed by ecophysiologists to collect accurate physiological data from free-ranging animals ethically and with an absolute minimum of impact. In turn, findings have the capacity to foster transformative advances in human health monitoring. Thus, we invite biomedical engineers and researchers to collaborate with the animal-tagging community to drive forward the advancements necessary to realize the full potential of both fields. This article is part of the theme issue 'Measuring physiology in free-living animals (Part II)'.

Spectral Determinant on Euclidean Isosceles Triangle Envelopes

We study extremal properties of the determinant of Friederichs selfadjoint Laplacian on the Euclidean isosceles triangle envelopes of fixed area as a function of angles. Small-angle asymptotics show that the determinant grows without any bound as an angle of triangle envelope goes to zero. We prove that the equilateral triangle envelope (the most symmetrical geometry) always gives rise to a critical point of the determinant and find the critical value. Moreover, if the area of envelopes is not too large, then the determinant achieves its absolute minimum only on the equilateral triangle envelope and there are no other critical points, whereas for sufficiently large area the equilateral triangle envelope corresponds to a local maximum of the determinant.

Improved Vertex Coloring With NbOₓ Memristor-Based Oscillatory Networks

The main focus of this paper is the presentation of reliable methods for the determination of the optimum coloring of a graph, commonly known in the literature as vertex coloring problem. It has been shown that networks of capacitively coupled oscillators can be used to solve vertex coloring problems. In this paper we address the negative impact of an unbalanced number of couplings for the oscillators on the performance of the network and compensate for this non-uniform coupling structure by an adjustment in the network itself. The negative effect of the memristor device-to-device variability of the NbO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> memristor on the array functionality will be investigated and reduced via an adaptation of the memristor operating point. The main improvement in network performance is achieved by setting up a control procedure allowing the network to bypass the local solutions and converge to the global one. Two strategies inspired by global optimization algorithms will be proposed to allow the network to overcome sub-optimal solutions, and find the solution corresponding to the absolute minimum of a performance measure function of the vertex coloring problem.

Dictionaries Integrated into English Learning Apps: Critical Comments and Suggestions for Improvement

Digital applications to assist language learning are becoming increasingly popular. They typically incorporate one or two dictionaries to improve the service so that users avoid leaving the app to consult external resources. This paper deals with the two dictionaries used in a learning app for Chinese learners of English. Initially, it describes the functioning of the app as well as the two dictionaries that have different roles in the app. It then focuses on the one that is integrated into the course texts and can be activated by clicking on a word or a multiword unit. A number of deficiencies are discussed such as inconsistent treatment of words and senses, data overload, difficult access, and inconvenient location of the pop-up window that displays the lexicographical items. These deficiencies may impact negatively on the learners' motivation and the learning process in general. The paper traces the problems to the database that sustains the dictionary as well as the design of the user interfaces that filter the data offered to the users. Finally, and inspired by the classical Chinese Xun Gu tradition, it suggests an alternative, context-adapted approach that breaks with traditional features of the dictionary article and reduces the content of the pop-up window to an absolute minimum. The idea is to avoid a consultation process that interrupts the learners' reading flow and focus on learning.

Entanglement and quantum strategies reduce congestion costs in Pigou networks

Pigou’s problem has many applications in real life scenarios like traffic networks, graph theory, data transfer in internet networks, etc. The two player classical Pigou’s network has an unique Nash equilibrium with the Price of Stability and Price of Anarchy agreeing with each other. The situation changes for the k person classical Pigou’s network with n being the total number of people. If we fix the behaviour of (n−2) people and assume that k persons take path P2 where k<(n−2) and the remaining take path P1, the minimum cost of Nash equilibrium becomes k dependent and we find a particular k for which the cost is an absolute minimum. In contrast to the two person classical Pigou’s network, the quantum two qubit Pigou’s network with maximal entanglement gives a lower cost for the Nash equilibrium. In contrast to k person classical Pigou’s network, its quantum version with the quantum miracle move strategy M has reduced cost for the Nash equilibrium. This has major implications for information transfer in both classical as well as quantum data networks. By employing entanglement and quantum strategies, one can significantly reduce congestion costs in quantum data networks.

Protocol to maintain ECT in COVID-19 pandemic

IntroductionElectroconvulsive therapy is a highly effective treatment for severe psychopharmacological resistant patients but it is also a procedure that involves open airway management and has been considered as an aerosol generating procedure. The COVID-19 pandemic, has resulted in reduction in ECT services internationally. The COVID-19 pandemic has dramatically and rapidly transformed hospitals in heavily affected areas, decreasing mental health services. The need to locate critical patients in spaces intended for anesthesia, where we usually administered ECT, has forced us to decrease the number of procedures and be highly selective. In the same way, continuation and maintenance ECT (m-ECT) have also been dramatically reduced. The risk of contagion urged us to develop a protocol involving other areas of the hospitalObjectivesTo create a safe circuit from admission to the hospital to the ECT including emergency room and psychiatric WardMethodsReview of the tliterature and published protocols Workshops with Preventive Medicine, Anaesthesia and Emergency Service to elaborate a protocol Submission of the protocol to Management of the HospitalResultsThe protocol (Figure 1) began with the screening for COVID-19 in every patient. If the PCR was (+) the patient was not excluded. We moved treatment from the PACU into the OR and if a patient tested positive It was determined that the ECT was administered in the ORThat was provided with negative pressure. Circuits were established within the Psychiatric Ward and in the areas of the hospital involved to reduce risks and patients remained isolated until negative test was confirmed The number of persons present in the treatment room was kept to the absolute minimum required and appropriate personal protective equipment was used, as prescribed by the WHOConclusionsWe must keep in mind treating the most vulnerable of our patients. ECT should be seen as an essential medical procedure and made availableDisclosureNo significant relationships.

Climate and environmental changes of the Lateglacial transition and Holocene in northeastern Siberia: Evidence from diatom oxygen isotopes and assemblage composition at Lake Emanda

A new dataset from Lake Emanda provides insights into climate and environmental dynamics in an extreme continental setting in northeastern Siberia. The δ18Odiatom record is supported by diatom assemblage analysis, modern isotope hydrology and atmospheric circulation patterns. The data reveal a relatively cold oligotrophic freshwater lake system persisting for the last ∼13.2 cal ka BP. Most recent δ18Odiatom (+21.5‰) combined with present-day average δ18Olake (−16.5‰) allows calculating Tlake (∼21 °C), reflecting summer conditions. Nonetheless, the δ18Odiatom variability is associated with changes in δ18Olake rather than with Tlake. An obvious shift of ∼2‰ in the δ18Odiatom record at 11.7–11.5 cal ka BP accompanied by significant changes in diatom assemblages reflects the onset of the Holocene. Relatively high δ18Odiatom during the Early Holocene suggests relatively warm and/or dry climate with associated evaporation effects. The absolute maximum in δ18Odiatom of +27.7‰ consistent with high values of diatom indices at ∼7.9–7.0 cal ka BP suggests a Mid Holocene Thermal Maximum. A continuous depletion in δ18Odiatom since ∼5.0 cal ka BP is interpreted as Middle to Late Holocene cooling reaching the absolute minimum at 0.4 cal ka BP (i.e. the Little Ice Age). An overall cooling trend (∼0.3‰ 1000 yr−1) throughout the Holocene follows decreasing solar insolation. The pattern of the Lake Emanda δ18Odiatom record is similar to that obtained from Lake El’gygytgyn suggesting a common “eastern” regional signal in both records, despite their hydrological differences. Presently, atmospheric moisture reaches the study region from the west and east with ∼40% each, as well as ∼20% from the north.

Povezanost površinske temperature mora i površinske temperature zraka: slučaj otoka Hvara (Jadransko more, Hrvatska)

Relationship of Sea Surface Temperature (SST) and Surface Air Temperature (SAT) measured at the small island of Hvar (Adriatic Sea) are examined on annual and monthly time scales using data for a period of 55 years (1964-2018). The following three SST and SAT indices were analysed: (1) absolute minimum; (2) mean; (3) absolute maximum. The results highlighted a statistically significant increasing trend for all three SST analysed annual indices. In the case of SAT absolute minimum increasing trend is not statistically significant. For the other two indices the trends are statistically significant at the level, p

The Effect of Attending Physical Rehabilitation After the First Acute Lateral Ankle Sprain on Static Postural Control in Patients With Chronic Ankle Instability.

Static postural control deficits are commonly documented among individuals with chronic ankle instability (CAI). Evidence suggests individuals with CAI who seek medical attention after an ankle sprain report fewer subjective symptoms. It is unknown if seeking medical attention and receiving supervised physical rehabilitation has a similar effect on objective outcomes, such as static postural control. To compare measures of single-limb postural control and center of pressure (COP) location between participants with CAI who did or did not self-report attending supervised rehabilitation at the time of their first lateral ankle sprain. Retrospective cohort. Laboratory. Patients (or Other Participants): Twenty-nine participants with CAI who did (n = 14) or did not (n = 15) self-report attending supervised rehabilitation. Self-reported attendance or not of supervised rehabilitation at the time of initial injury. Participants performed three 20-second trials of single-limb stance on a force plate with eyes open. Main outcome measures included the COP velocities, time-to-boundary (TTB) absolute minima, mean of TTB minima, and SD of TTB minima in the anteroposterior and mediolateral directions. The spatial distribution of the COP data points under the foot was quantified within 4 equally proportional sections labeled anteromedial, anterolateral, posteromedial, and posterolateral. Participants who reported attending supervised rehabilitation after their initial ankle sprain had a lower COP velocity in the anterior-posterior direction (P = .030), and higher TTB anterior-posterior absolute minimum (P = .033) and mean minima (P = .050) compared with those who did not attend supervised rehabilitation. Among individuals with CAI, not attending supervised rehabilitation at the time of initial injury may lead to worse static postural control outcomes. Clinicians should continue advocating for patients recovering from an acute ankle sprain to seek medical attention and provide continued care in the form of physical rehabilitation.

Force production during maximal front crawl tethered swimming: exploring bilateral asymmetries and differences between breathing and non-breathing conditions

ABSTRACT The present study focused on propulsive forces applied during tethered swimming. The main aims were to identify asymmetries between dominant and non-dominant arms, quantify the effect of breathing on force application and, explore any association between each variable and swimming performance. Fifteen regional level swimmers completed a maximal front crawl tethered swimming test, with maximal kicking, under four conditions: 1) Dominant arm strokes only, no breathing; 2) non-dominant arm strokes only, no breathing; 3) full stroke, no breathing; 4) full stroke, breathing on the preferred side. The outcome variables were: absolute and normalised (force divided by body mass) minimum, mean and maximum force; stroke cycle time and; impulse. The symmetry index was also calculated, and all variables were correlated with the swimmers’ season best times in 50 m front crawl. Some bilateral force asymmetries were found, but they did not always favour the dominant side and were not directly linked with swimming performance. There was no strong evidence that force production is higher on the dominant side or that symmetry in force production affects performance. Despite the longer stroke cycle times when breathing, the breathing actions did not affect force production. Faster swimmers often produced higher maximum force values and, sometimes, higher mean force values.

Vacuum structure of the \u21242 symmetric Georgi-Machacek model

We discuss the vacuum structure of a version of the Georgi-Machecek model with an exact ℤ2 symmetry acting on the triplet fields. Besides the usual custodial-symmetric model, with ρ = 1 at tree-level, a model with a dark matter candidate is also viable. The other phases of the model lead to electric charge breaking, a wrong pattern of electroweak symmetry breaking or to ρ ≠ 1 at tree-level. We derive conditions to have an absolute minimum in each of the two viable phases, the custodial and the dark matter phases.

Seasonal and Vertical Variability of Currents Energy in the Sub-Mesoscale Range on the Black Sea Shelf and in Its Central Part

Purpose. The study is aimed at investigating seasonal variability and vertical distribution of the sub-mesoscale currents energy (scales L = 1 … 10 km, T = 1 … 10 days) in the deep and shelf zones of the Black Sea. Methods and Results. The study is based on the spectral analysis of the results obtained from the NEMO model numerical calculations performed with high spatial resolution 1 km. The analysis shows that the seasonal variability of the submesoscale energy is significantly different in deep and shelf zones of the basin. At the same time, in both regions, seasonal variation of energy of the sub-mesoscale currents with scales L &lt; 10 rm (Esp) is in good agreement with that of the density fluctuations on the same scales. In the central part of the sea, the high values of Esp are concentrated in the upper mixed layer throughout the whole year. The Esp peak is observed in winter at the depths 0–40 m, which indicates the important role of baroclinic instability induced by the inhomogeneous distribution of the mixed layer depth (MLD) in the generation of sub-mesoscale processes. At the same time, in February in the central part of the northwestern shelf, an absolute minimum of (Esp) is observed. This minimum is caused by the complete mixing and barotropization of the water column. The Esp maximum values are observed in the shelf in September – October. This is related to the intensification of the brackish water transport from the river mouths by mesoscale eddies. In the autumn period high values of Esp in the shelf and deep part of the basin are observed in the deeper layer, compare to summer months .Variability of the Esp vertical distribution coincides to the time variation of MLD. Variability of the submesoscale energy is of a pulsating character with the short-term intensifications and weakenings. Such variability is significantly related to the passing of the mesoscale fronts and the cross-shelf water transport caused by the eddies and upwellings, which lead to the increase of the baroclinic instability. Conclusions. Analysis of the seasonal and vertical variability of the submesoscale currents in the Black Sea deep and shelf zones evidences about the decisive role of the baroclinic instability triggered mainly by the heterogeneity of MLD on their dynamics.

Harmonization of Protocols for Multi-Species Organoid Platforms to Study the Intestinal Biology of Toxoplasma gondii and Other Protozoan Infections.

The small intestinal epithelium is the primary route of infection for many protozoan parasites. Understanding the mechanisms of infection, however, has been hindered due to the lack of appropriate models that recapitulate the complexity of the intestinal epithelium. Here, we describe an in vitro platform using stem cell-derived intestinal organoids established for four species that are important hosts of Apicomplexa and other protozoa in a zoonotic context: human, mouse, pig and chicken. The focus was set to create organoid-derived monolayers (ODMs) using the transwell system amenable for infection studies, and we provide straightforward guidelines for their generation and differentiation from organ-derived intestinal crypts. To this end, we reduced medium variations to an absolute minimum, allowing generation and differentiation of three-dimensional organoids for all four species and the subsequent generation of ODMs. Quantitative RT-PCR, immunolabeling with antibodies against marker proteins as well as transepithelial-electrical resistance (TEER) measurements were used to characterize ODM’s integrity and functional state. These experiments show an overall uniform generation of monolayers suitable for Toxoplasma gondii infection, although robustness in terms of generation of stable TEER levels and cell differentiation status varies from species to species. Murine duodenal ODMs were then infected with T. gondii and/or Giardia duodenalis, two parasites that temporarily co-inhabit the intestinal niche but have not been studied previously in cellular co-infection models. T. gondii alone did not alter TEER values, integrity and transcriptional abundance of tight junction components. In contrast, in G. duodenalis-infected ODMs all these parameters were altered and T. gondii had no apparent influence on the G. duodenalis-triggered phenotype. In conclusion, we provide robust protocols for the generation, differentiation and characterization of intestinal organoids and ODMs from four species. We show their applications for comparative studies on parasite-host interactions during the early phase of a T. gondii infection but also its use for co-infections with other relevant intestinal protozoans.

Cost Optimization for Big Data Workloads Based on Dynamic Scheduling and Cluster-Size Tuning

Analytical data processing has become the cornerstone of today's businesses success, and it is facilitated by Big Data platforms that offer virtually limitless scalability. However, minimizing the total cost of ownership (TCO) for the infrastructure can be challenging. We propose a novel method to build resilient clusters on cloud resources that are fine-tuned to the particular data processing task. The presented architecture follows the infrastructure-as-a-code paradigm so that the cluster can be dynamically configured and managed. It first identifies the optimal cluster size to perform a job in the required time. Then, by analyzing spot instance price history and using ARIMA models, it optimizes the schedule of the job execution to leverage the discounted prices of the cloud spot market. In particular, we evaluated savings opportunities when using Amazon EC2 spot instances comparing to on-demand resources. The performed experiments confirmed that the prediction module significantly improved the cost-effectiveness of the solution – up to 80% savings compared to the on-demand prices, and at the worst-case, 1% more cost than the absolute minimum. The production deployments of the architecture show that it is invaluable for minimizing the total cost of ownership of analytical data processing solutions. • Extended cloud architecture that dynamically selects and schedules resources. • Evaluated several spot price prediction models to forecast future prices. • Confirmed feasibility of short-term spot price prediction through real data from AWS. • Cost savings of up to 80% compared to on-demand and within 1% of the absolute minimum.