The purpose of the current study is to validate the Attitudes Related to Trauma-Informed Care Scale (ARTIC; Baker et al., 2016), a measure of trauma-informed care (TIC) attitudes for human service/health providers and educators. The current study with 1,395 human services/health providers and educators from 17 settings examined the factor structure, reliability, and construct validity of the ARTIC. The 7-factor structure of the ARTIC-45 and the 5-factor structure of the ARTIC-35 were replicated with regard to the absolute fit indices though they failed to meet the cutoff for the incremental fit indices, likely due to the complexity of the measure. Internal consistencies ranged from acceptable to excellent across the instrument's forms, subscales, and versions. Providing support for construct validity, ARTIC scores were predictably related to familiarity with and knowledge about TIC, compassion satisfaction, burnout, and secondary traumatic stress, with effect sizes typically in the medium range. However, predicted relationships between ARTIC scores and training in TIC and trauma-sensitive school elements were not evident. The ARTIC is a direct, efficient, and cost-effective measure of attitudes related to TIC. This study further demonstrates the reliability of the ARTIC and provides some support for its validity. Evaluating the favorability of staff attitudes about TIC plays a key role in ensuring readiness for, effectiveness of, and sustainability of TIC in systems. (PsycInfo Database Record (c) 2021 APA, all rights reserved).