Healing Ability Research Articles

Integrated in silico and in vitro exploration of the anti-VEGFR-2 activities of a semisynthetic xanthine alkaloid inhibiting breast cancer.

This study presents T-1-NBAB, a new compound derived from the natural xanthine alkaloid theobromine, aimed at inhibiting VEGFR-2, a crucial protein in angiogenesis. T-1-NBAB's potential to interacts with and inhibit the VEGFR-2 was indicated using in silico techniques like molecular docking, MD simulations, MM-GBSA, PLIP, essential dynamics, and bi-dimensional projection experiments. DFT experiments was utilized also to study the structural and electrostatic properties of T-1-NBAB. Computational analysis was performed to predict the ADME-Tox profiles of T-1-NBAB. After semisynthesis, the in vitro results showed that T-1-NBAB effectively inhibits VEGFR-2, with an IC50 of 0.115 μM, compared to sorafenib's 0.0591 μM. In vitro tests also demonstrated significant activity of T-1-NBAB against breast cancer cell lines MCF7 and T47D, with IC50 values of 16.88 μM and 61.17 μM, respectively, and high selectivity. Importantly, T-1-NBAB induced early and late apoptosis in MCF7 cells, indicating its potential as a strong anticancer agent. Additionally, T-1-NBAB reduced the migration and healing abilities of MCF7 cells, suggesting it could be a promising anti-angiogenic agent. Overall, these findings suggest that T-1-NBAB is a promising lead compound for further research as a potential treatment for breast cancer.

Rosae Multiflorae Fructus Extracts Regulate the Differentiation and Vascular Endothelial Cell-mediated Proliferation of Keratinocytes.

Keratinocytes are the primary component of the epidermis, so maintaining the precise balance between proliferation and differentiation is essential for conserving epidermal structure and function. Rosae multiflorae fructus extract (RMFE) has wide application in the cosmetic industry, but the molecular mechanisms underlying beneficial effects on keratinocytes are still not fully understood. In this study, we found that RMFE promoted epidermal differentiation and enhanced the barrier function of normal human epidermal keratinocytes (NHEKs) and three-dimensional epidermis model in culture. In addition, RMFE promoted human umbilical vein endothelial cell (HUVEC) proliferation and angiogenesis, whereas the conditioned medium from RMFE-treated HUVECs further promoted NHEK proliferation and increased wound healing ability. Analysis of constituent bioactivities identified a quercetin derivative as a potential mediator of NHEK and HUVEC responses to RMFE. Taken together, these results suggest that RMFE enhances epidermal functions through both direct effects on keratinocytes and indirect effects mediated by endothelial cells.

Study of the Wound-Healing Activity of a New Drug Derived from Cobalt Polyacrylate

Previously we suggested a new pharmaceutical derived from coordination complex of Co3+ with polyacrylic acid (PAA) exhibiting hemostatic and microbicidal activity, namely Hestatin. Differences in the physiological activity of Hestatin synthesized from PAA 10 kDa (Hestatin 10) and 200 kDa (Hestatin 200) were shown. We tested the acute toxicity of Hestatin and its effect on the healing rate of sterile wounds in rats. Free 10 kDa PAA, emulsion wax, emulsion wax carrying resveratrol, and dexpanthenol were tested for comparison. Hestatin 10 exhibited no acute toxicity when administered intragastrically at dosages of 5 g per kg. Hestatin 10 surpassed all tested drugs in its wound healing ability. Histological analysis of skin sections of rats in the area of healing defects showed an increased rate of synthesis of reticular fibers compared to the placebo. In the early stages of wound healing (inflammatory phase), Hestatin 10 stimulated taxis of mast cells (MCs) to the wound bottom but not to the wound perimeter. At the final stage of wound healing (remodeling phase), Hestatin 10 promoted MC evacuation from the skin defect area. This effect is the opposite of the well-known wound-healing agents (dexpanthenol and resveratrol), which enhance MC infiltration into the defect area in the remodeling phase.

Osteoblastic Differentiation and Mitigation of the Inflammatory Response in Titanium Alloys Decorated with Oligopeptides.

Under benign conditions, bone tissue can regenerate itself without external intervention. However, this regenerative capacity can be compromised by various factors, most importantly related with the extent of the injury. Critical-sized defects, exceeding the body's natural healing ability, demand the use of temporary or permanent devices like artificial joints or bone substitutes. While titanium is a widely used material for bone replacement, its integration into the body remains limited. This often leads to the progressive loosening of the implant and the need for revision surgeries, which are technically challenging, are commonly associated with high complication rates, and impose a significant economic burden. To enhance implant osseointegration, numerous studies have focused on the development of surface functionalization techniques to improve the response of the body to the implant. Yet, the challenge of achieving reliable and long-lasting prostheses persists. In this work, we address this challenge by applying a robust and versatile biofunctionalization process followed by the decoration of the material with oligopeptides. We immobilize four different peptides (RGD, CS-1, IKVAV, PHSRN) on R-THAB® functionalized surfaces and find them to be highly stable in the long term. We also find that RGD is the best-performing peptide in in vitro cell cultures, enhancing adhesion, proliferation, and osteogenic differentiation of mesenchymal stem cells. To assess the in vivo effect of RGD-decorated Ti-6Al-4V implants, we develop a calvarial model in murine hosts. We find that the RGD-decoration remains stable for 1 week after the surgical procedure and reduces post-implantation macrophage-related inflammation. These results highlight the potential of peptide decoration on R-THAB® functionalized surfaces to expedite the development of novel metallic biomaterials with enhanced biocompatibility properties, thereby advancing the field of regenerative medicine.

Senescent retinal pigment epithelial cells promote angiogenesis in choroidal neovascularization via the TAK1/p38 MAPK pathway.

Senescent retinal pigment epithelial cells play a key role in neovascular age-related macular degeneration (nAMD); however, the mechanisms underlying the angiogenic ability of these cells remain unclear. Herein, we investigated the effects of the senescent adult retinal pigment epithelial cell line-19 (ARPE-19) on wound healing, cell migration and survival, and tube formation abilities of human umbilical vein endothelial cells (HUVECs). Additionally, we used Brown Norway rats to establish a laser-induced choroidal neovascularization (CNV) model for further nAMD-related studies. We found that the wound healing, cell migration, and tube formation abilities of HUVECs were significantly enhanced following culture in conditioned media from senescent ARPE-19cells; this was attributed to the activation of the transforming growth factor β-activated kinase 1 (TAK1)/p38 MAPK pathway. Consistently, we found that the TAK1 inhibitors 5Z-7-oxozeaenol and takinib reversed the effects of conditioned media from senescent ARPE-19cells on the wound healing, migration, survival, and tube formation abilities of HUVECs. We further investigated the therapeutic effects of 5Z-7-oxozeaenol on the laser-induced CNV rat model. We found that TAK1 was activated in IB4+ areas in laser-induced CNV lesions; inhibiting the activity of TAK1 using 5Z-7-oxozeaenol significantly alleviated CNV lesion formation and fluorescein leakage in fundus fluorescein angiography and greatly improved a-waves, b-waves, and OP values, as recorded by electroretinography. Thus, senescent RPE cells may promote angiogenesis via the TAK1/p38 MAPK pathway. Further, inhibiting TAK1 expression alleviates pathological neovascularization and improves retinal function in a laser-induced CNV rat model, highlighting the therapeutic potential of this approach for treating nAMD.

Unlocking the Healing Potential: A Comprehensive Review of Ecology and Biology of Medical-Grade Honey in Wound Management and Tissue Regeneration.

Honey has long been studied for its healing abilities in wound care. This narrative review examines its properties and their impact on wound healing, particularly its ability to accelerate wound closure and promote tissue regeneration. The review focuses on how honey's botanical origins affect its medical properties and wound-healing capabilities. Finally, clinical studies on honey's effectiveness in wound healing were reviewed compared to traditional treatments. Relevant keywords were searched in databases, yielding 1250 documents. After excluding nonrelevant sources, 450 documents were refined, and 167 articles were selected based on thematic alignment and originality. Data extraction focused on study design, intervention details, and outcomes, with quality assessed using standardized criteria. The study adhered to CONSORT and SANRA guidelines to ensure methodological rigor and reporting transparency. Honey-based medical products have demonstrated significant antibacterial, anti-inflammatory, and tissue-regenerative properties, making them highly effective in improving wound healing outcomes, particularly in chronic and burn wounds. These products have also been shown to reduce infection rates and hospital stays. While some studies have reported positive outcomes in accelerating the healing process, others have found no significant difference compared to conventional treatments. Medical-grade honey (MGH) holds potential for wound care due to its versatility, though variations in its composition present challenges. Further research is needed to optimize its clinical use. The effectiveness of MGH in wound healing remains debated, with mixed results from trials. Genetic modification of bees to enhance MGH's properties could make it more competitive against conventional treatments. Honey-based medications could reduce costs, improve energy efficiency, and have minimal side effects. Rigorous research is necessary to determine optimal use and fully unlock MGH's potential, which could revolutionize wound management globally.

Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice

Yeast β-glucan exhibits dramatic potential as wound healing regent owing to its various biological properties including immunomodulatory, anti-inflammatory, and antioxidant. But, the poor water solubility of yeast β-glucan limits its application. In this study, some carboxymethylated yeast β-glucans (CMGs) with different substitution degree was prepared. The effect of substitution degree on biological activities of carboxymethylated yeast β-glucan was investigated using zebrafish model. CMG3 with substitution degree 0.55 showed anti-inflammatory, antioxidant, and immunomodulatory activities in zebrafish. CMG3 also showed potential ability to promote angiogenesis and caudal fin regeneration. The cytotoxicity of CMG3 was investigated on L929 cells and the effect of CMG3 on cell migration was studied by scratch test. The hemolysis ratio of CMG3 was determined, and the in vitro antioxidant activity was studied. Next, CMG3 was used to prepare a sprayable hydrogel through a simple method, and the wound healing ability was studied using a streptozotocin-induced diabetic mice model. The results indicated that CMG3-based sprayable hydrogel could accelerate wound healing in a diabetic mice and influence the expression of biomarkers related to inflammatory, macrophage polarization, and angiogenesis.

NSAIDs in Sports Medicine: Balancing Quick Relief with Long-Term Health Impacts

Introduction and PurposeNonsteroidal anti-inflammatory drugs (NSAIDs) are regularly used in sports medicine to manage pain and inflammation caused by acute and chronic injuries. Their mechanism involves inhibition of cyclooxygenase (COX) enzymes, reducing the body’s natural inflammatory response. This review aims to analyze the effects of NSAID use in sports contexts, focusing on short- and long-term benefits and risks, specifically concerning tissue healing, athletic performance, and general health outcomes.Materials and MethodsWe reviewed studies from PubMed, Google Scholar and ResearchGate, focusing on key search terms including NSAIDs in sports injuries, NSAID mechanism, short-term and long-term effects of NSAIDs, athletic performance and NSAID, pain management in sports, NSAID side-effects and NSAID alternativesConclusionsShort-term NSAID use can provide effective pain relief and support functional recovery, benefiting professional and recreational athletes in their sports. However, prolonged use may decrease natural tissue healing abilities, increase susceptibility to various side effects, and potentially affect long-term performance. Alternative pain management strategies are recommended to complement NSAID use, providing athletes with safer, long-term options for recovery. This review highlights the need for a balanced approach to NSAID usage, recommending cautious decision making among sportspeople, coaches, and healthcare workers.

Investigating the Role of Oxidative Stress and Immune Response in Rheumatoid Arthritis

Background:Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune sickness characterized with the aid of persistent joint infection, leading to joint damage and useful impairment. The pathogenesis of RA is thought to be encouraged with the aid of each genetic and environmental elements, with immune device dysregulation playing a valuable role in ailment progression. Increasing proof shows that oxidative pressure, which results from an imbalance between reactive oxygen species (ROS) and antioxidant defenses, contributes to the inflammatory techniques and tissue harm seen in RA. Objectives: This take a look at aimed to investigate the relationship among oxidative pressure and immune machine dysregulation inside the pathogenesis and progression of rheumatoid arthritis (RA). Methodology:This look at turned into conducted from 1st April to 30 November 2024 and protected a total of 200 participants were covered inside the examiner, along with 150 RA patients (75 male and 75 female) and 50 wholesome controls (25 male and 25 lady). The individuals’ ages ranged from 35 to 75 years. Blood samples were collected from the outpatient clinics at Tikrit Teaching Hospital among April and October 2024, and RA patients have been identified primarily based on medical criteria with the aid of the attending physicians. Oxidative strain markers, along with malondialdehyde (MDA) and glutathione (GSH), and pro-inflammatory cytokines, together with IL-1β, IL-17, and TNF-α, had been measured. Disease interest became assessed the usage of the Disease Activity Score 28 (DAS28). Results:The effects revealed that each male and female RA patients had extensively higher levels of MDA and decrease GSH tiers compared to the healthy manage institution. Moreover, seasoned-inflammatory cytokines (IL-1β, IL-17, and TNF-α) had been appreciably improved in RA sufferers, indicating a country of chronic infection. The DAS28 ratings confirmed that a majority of the RA patients had moderate to excessive disorder hobby. No good sized gender-based variations have been discovered in oxidative strain markers, cytokine stages, or sickness interest rankings. Conclusions: Our take a look at highlights the essential interaction among oxidative strain and immune dysregulation in RA. The determined correlations among oxidative markers, pro-inflammatory cytokines, and disease severity underscore the capability for focused on these pathways inside the management of RA. Future studies are warranted to in addition explore the healing ability of antioxidant-primarily based and anti-cytokine treatments in ameliorating RA-associated inflammation and joint damage. Further research is needed to explore the underlying mechanisms and capacity treatments focused on oxidative stress and immune modulation in RA.

Investigating the Role of Oxidative Stress and Immune Response in Rheumatoid Arthritis

Background:Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune sickness characterized with the aid of persistent joint infection, leading to joint damage and useful impairment. The pathogenesis of RA is thought to be encouraged with the aid of each genetic and environmental elements, with immune device dysregulation playing a valuable role in ailment progression. Increasing proof shows that oxidative pressure, which results from an imbalance between reactive oxygen species (ROS) and antioxidant defenses, contributes to the inflammatory techniques and tissue harm seen in RA. Objectives: This take a look at aimed to investigate the relationship among oxidative pressure and immune machine dysregulation inside the pathogenesis and progression of rheumatoid arthritis (RA). Methodology:This look at turned into conducted from 1st April to 30 November 2024 and protected a total of 200 participants were covered inside the examiner, along with 150 RA patients (75 male and 75 female) and 50 wholesome controls (25 male and 25 lady). The individuals’ ages ranged from 35 to 75 years. Blood samples were collected from the outpatient clinics at Tikrit Teaching Hospital among April and October 2024, and RA patients have been identified primarily based on medical criteria with the aid of the attending physicians. Oxidative strain markers, along with malondialdehyde (MDA) and glutathione (GSH), and pro-inflammatory cytokines, together with IL-1β, IL-17, and TNF-α, had been measured. Disease interest became assessed the usage of the Disease Activity Score 28 (DAS28). Results:The effects revealed that each male and female RA patients had extensively higher levels of MDA and decrease GSH tiers compared to the healthy manage institution. Moreover, seasoned-inflammatory cytokines (IL-1β, IL-17, and TNF-α) had been appreciably improved in RA sufferers, indicating a country of chronic infection. The DAS28 ratings confirmed that a majority of the RA patients had moderate to excessive disorder hobby. No good sized gender-based variations have been discovered in oxidative strain markers, cytokine stages, or sickness interest rankings. Conclusions: Our take a look at highlights the essential interaction among oxidative strain and immune dysregulation in RA. The determined correlations among oxidative markers, pro-inflammatory cytokines, and disease severity underscore the capability for focused on these pathways inside the management of RA. Future studies are warranted to in addition explore the healing ability of antioxidant-primarily based and anti-cytokine treatments in ameliorating RA-associated inflammation and joint damage. Further research is needed to explore the underlying mechanisms and capacity treatments focused on oxidative stress and immune modulation in RA.

AI-Guided Inverse Design and Discovery of Recyclable Vitrimeric Polymers.

Vitrimer is a new, exciting class of sustainable polymers with healing abilities due to their dynamic covalent adaptive networks. However, a limited choice of constituent molecules restricts their property space and potential applications. To overcome this challenge, an innovative approach coupling molecular dynamics (MD) simulations and a novel graph variational autoencoder (VAE) model for inverse design of vitrimer chemistries with desired glass transition temperature (Tg) is presented. The first diverse vitrimer dataset of one million chemistries is curated and Tg for 8,424 of them is calculated by high-throughput MD simulations calibrated by a Gaussian process model. The proposed VAE employs dual graph encoders and a latent dimension overlapping scheme which allows for individual representation of multi-component vitrimers. High accuracy and efficiency of the framework are demonstrated by discovering novel vitrimers with desirable Tg beyond the training regime. To validate the effectiveness of the framework in experiments, vitrimer chemistries are generated with a target Tg = 323 K. By incorporating chemical intuition, a novel vitrimer with Tg of 311-317 K is synthesized, experimentally demonstrating healability and flowability. The proposed framework offers an exciting tool for polymer chemists to design and synthesize novel, sustainable polymers for variousapplications.

Reproductive performance of Channa striata in wetland ecosystems: a fuzzy logic approach to water quality and eco-climatic factors for long-term sustainable management and aquaculture advancement.

The striped snakehead, Channa striata, is commercially and nutritionally important due to its medicinal properties, such as wound healing and antimicrobial abilities. This study investigated the reproductive biology of C. striata in relation to hydro-climatic changes using a fuzzy logic approach for long-term management in the wetland ecosystem (Gajner beel), Bangladesh. A total of 1200 C. striata individuals were collected monthly during January to December 2019 using various fishing methods. Measurements of total length (TL), body weight (BW), and gonad weight (GW) were taken with a precision of 0.01cm and 0.01g, respectively. The GSI (gonadosomatic index in %), MGSI (modified gonadosomatic index in %), and DI (Dobriyal index) were used to determine size at sexual maturity (Lm) and the spawning season. Results revealed a wide range of TL, ranging from 12.7 to 46cm. The Lm was estimated between 21.7 and 25.33cm based on various model as TL vs. GSI, MGSI, and DI; TL-fork length (FL) regressions; and maximum length (Lmax) for this species. The spawning period was observed from April to July, peaking in May, indicated by higher GSI, MGSI, and DI values. Eco-climatic factors showed a significant relationship with GSI, except for total dissolved solids (TDS). Rising temperatures and declining rainfall could delay spawning of C. striata. A fuzzy logic approach was employed to predict future GSI changes over extended periods (e.g., 50 or 60years), enabling anticipation of shifts in the timing and duration of the spawning season. Finally, the findings of our research might be effective to carry out specific conservation efforts of C. striata in response to climate variability in sub-tropical water.

Treatment of Otitis Externa in Cats (<em>Malassezia</em> sp): Epidemic Genius

External otitis is one of the primary complaints affecting small animals. Traditional treatments, such as antibiotics and topical medications, have varying degrees of success and often result in side effects or incomplete resolution of symptoms. Therefore, the search for more effective and safer treatments is crucial. Homeopathic medicine offers an alternative approach, using substances that stimulate the body's innate healing abilities by considering the individual’s specific symptoms, temperament, and overall health to promote lasting healing. This study aims to evaluate the efficacy of homeopathic treatment for external otitis in cats by examining clinical outcomes and providing insights for future treatment protocols. Eight female cats with pruritus were examined. Upon examination and plate analysis, many Malassezia sp. were observed. Based on the clinical signs demonstrated by the animals, symptoms were selected for repertorization and medication choice (Epidemic Genius). The eight cats received Apis mellifica 12 cH, Malassezia sp. 30 cH in their drinking water for 15 days, and Calendula Glyceroleum for external use for daily cleaning. After one week of treatment, new samples were collected for analysis of the ear canal and testing for Malassezia sp. It was found that 100% of the animals no longer presented Malassezia sp. in the examination or had an extremely low number (considered normal). Homeopathy, through treatment with Epidemic Genius, proved to be effective in treating the eight cats.

Aging negatively affects postoperative recovery of biomechanical strength through decreased recruitment of Scx+/Sox9+ enthesis-related progenitors after rotator cuff repair in rats

BackgroundAlthough older adult patients have a higher retear rate after rotator cuff (RC) repair, the influence of aging on the healing process remains unclear. During mouse enthesis development, a multipotent progenitor co-expressing scleraxis (Scx) and SRY-box 9 (Sox9) contributes to enthesis formation. Scx+/Sox9+ cells may act as enthesis progenitors even during postnatal healing, and their number decreases with maturation. However, the pathophysiology of decreased RC healing ability due to aging remains unclear. We aimed to evaluate the effects of aging on tendon-to-bone healing after surgical RC repair in rats through biomechanical and histological analyses of Scx+ and Sox9+ progenitors in a ScxGFP transgenic rat model. MethodsThis was a controlled laboratory study. Adult Sprague-Dawley rats (n = 111) underwent unilateral surgery for supraspinatus tendon repair immediately after transection. The rats were divided into aged (95 weeks old) and control (12 weeks old) groups. The effects of aging were assessed using biomechanical tests at 6 weeks postoperatively and histologically at 2 and 6 weeks postoperatively. ScxGFP transgenic rats were used for the immunohistochemical assessment of Scx- and Sox9-expressing progenitors during the repair process. Sox9, Scx, and tenomodulin expression was assessed using real-time reverse transcription polymerase chain reaction. ResultsIn the biomechanical test at 6 weeks postoperatively, the aged group had lower ultimate load to failure (Control: 20.4 ± 6.1 N, Aged: 14.9 ± 6.6 N, P = 0.007), stiffness (Control: 16.1 ± 6.2 N/mm, Aged: 12.6 ± 5.5 N/mm, P = 0.023), and ultimate stress to failure (Control: 6.0 ± 3.4 N/mm2, Aged: 3.4 ± 2.5 N/mm2, P < 0.001) than the control group. The total histological score of the aged group was lower than that of the control group at 6 weeks postoperatively (Control: 8.8 ± 0.8, Aged: 5.8 ± 0.4, P = 0.029). Immunohistochemistry tests showed that the percentages of Sox9+ (Control: 6.6 ± 1.1, Aged: 2.0 ± 1.0, P = 0.029) and Scx+/Sox9+ (Control: 3.6 ± 0.8, Aged: 1.1 ± 0.6, P = 0.029) cells at the reparative site were lower in the aged group than in the control group at 2 weeks postoperatively. ConclusionIn a rat RC tendon-to-bone healing model, the decreased recruitment of Scx+/Sox9+ enthesis-related progenitor cells in the early phase may be associated with delayed reparative tissue remodeling in aging animals. These findings encourage the development of therapeutic strategies that biologically promote healing and reduce postoperative retears in older adult patients.

The Effect of Routine Recitation on The Self Healing Ability of Jama'ah in Bandar Klippa Village

This research is motivated by daily problems experienced by the community such as stress, anxiety, and depression which are feared to become serious problems if not addressed properly. Therefore, the ability to overcome this is needed, such as self-healing therapy with a religious approach through routine recitation activities. The purpose of this study is to see the effect of routine recitation activities on the self- healing ability of the congregation. This study uses a quantitative method of descriptive correlation. The population of this study was the recitation congregation at the Umar Bin Abdul Aziz mosque, totaling 80 people, the sample was 40 people who were determined by the Slovin formula and selected based on purposive sampling technique. The instrument used was a questionnaire distributed after the congregation finished attending the recitation and in-depth Interviews with several worshipers. Data analysis using IBM SPSS statistical software using simple regression analysis techniques. The results showed that the congregation became calmer and could reduce the level of stress they experienced by being pleased with Allah's decree, smiling, being grateful, praying, thinking positively (husnudzon) and being patient. Thus, it is hoped that every community will participate in recitation activities in order to add insight and train self-healing abilities.

Self-assembled Arginine-Glycine-Aspartic Acid Mimic Peptide Hydrogels as Multifunctional Biomaterials for Wound Healing.

Clinical management of nonhealing ulcers requires advanced materials that can enhance wound closure rates without relying on the release of drugs or other growth factors to obviate systemic deleterious side effects. In our previous work, we synthesized an integrin-binding cell adhesive MNH2 {Fmoc-FFβAR(K)βA-NH2 consisting of an RGD mimic, [R(K)], with an amide terminus}, MOH {Fmoc-FFβAR(K)βA-OH consisting of an RGD mimic, [R(K)], with acid terminus}, and MR (Fmoc-FFβARGDβA-NH2 consisting of an RGD peptide, reference) with multifunctional activity. Here, we reported the synthesis, characterization, and performance of a reversed derivative, R-MNH2 (Fmoc-FFβA(K)RβA-NH2 consisting of an RGD mimic, [K(R)], with an amide terminus) of an antimicrobial cell adhesive peptide, MNH2. Both peptides (MNH2 and R-MNH2) were found to interact with αvβ3 integrin, as shown by docking studies; however, they differed in cell adhesive properties, hydrogel formation, and antimicrobial efficacy. Later, the wound healing ability of a series of RGD/RGD peptide mimics (MR, R-MNH2, MNH2, and MOH) was studied in a methicillin-resistant Staphylococcus aureus (MRSA)-infected Balb/c mouse model. All studied peptides showed cell adhesion and wound healing properties; however, only the amide-terminal RGD peptide mimic, MNH2, and its reversed derivative, R-MNH2, showed antimicrobial activity in both in vitro and in vivo studies. Of these, MNH2 showed the highest integrin-mediated spreading, migration, and proliferation of dermal cells in vitro as well as in vivo. Therefore, the MNH2 peptide mimic represents a paradigm shift in the development of dermoconductive strategies to treat chronic wounds.

A Boat-Paddle-Like Molecule Binder with Twining-Blocked and Ultrafast Self-Healing Functionalities for Stable Silicon Anodes.

Self-healing binder is a prospective and efficient strategy to alleviate volume expansion of silicon (Si) anodes. However, excellent mechanical strength and healing ability tend to be mutually exclusive, due to enhanced tensile stress limit by twining polymer chains, while inhibiting polymer diffusion rate and inducing healing failure by blocked chains. Herein, inspired by the planning course of boat and paddles, a novel self-healing binder (PVA-4FBA-PEI) is designed and synthesized with mobile parallel structure and twining-blocked characteristics. The boat-like intermediate (4-formylphenyl) boronic acid (4FBA) blocks entanglement of poly(vinyl alcohol) (PVA) and poly(ethyleneimine) (PEI) chains, where two parallel chains as paddles can simultaneously form to ensure rapid diffusion of chains during bond breakage. Remarkably, it endows dynamic synergistic covalent bonds via C═N and B─O─C junctions within 4FBA, providing the binder with an ultrafast self-healing time of merely 2 min. Moreover, the binder integrates superior plasticity and flexibility of each chain, showing a high tensile strength (14.4 MPa) and stretchability (1163%) among state-of-the-art polymer binders, thus significantly improving structural integrity and electrochemical stability of Si anode during cycling. This work proposes a dynamic reversible structure via figurative molecular coordination, affording a rational viewpoint on synergetic functionalities of polymer binders for Si anodes.

Assessment of Lighting in Medical Facilities from an Occupational Health Perspective

ABSTRACT Although workplace lighting improves employee satisfaction and performance, it may also harm health. It has been established that the lighting in hospitals influences the productivity of the staff. and patient healing ability. This study aimed to assess hospital lighting conditions and determine their impacts. During the first part of the study, lighting measurements were taken and evaluated in compliance with the TS EN 12,464-1: 2021–09 criteria in October and November of 2021. In the second step, 360 people were surveyed to evaluate employee opinions on lighting conditions. The maximum and minimum lighting levels were recorded at different hospital locations. Most participants worked in night shift, with an average age of 36.19 years and an average working time of 9 years. Overall, it was reported that the working and circulation spaces were well-lit. However, falls, cuts, fractures, sprains, bruises, and contusions were the most common lighting-related problems reported by the participants. According to the results, 15.5% of the participants experienced an occupational accident due to lighting. Therefore, modifying hospital lighting standards and implementing steps to protect personnel health are critical.

An Overview of the Osteogenic potential of Indian herb Cissus quadrangularis (Veldt Grape)

Cissus quadrangularis (CQ) is a widely known herb in India and southern part of India in particular. The bone healing ability of CQ is outlined in Ayurvedic medicine and other ancient medical texts. Bone loss in elderly especially osteoporosis is a public health problem across the globe. Bioactive molecules present in medicinal plants like CQ helps not only in bone healing but also in improving overall health. CQ is suggested as a plant herb in treatment of bone fractures and bone loss as it is safe or usage and side effects are not identified so far.However, research on the effect of CQ in humans are few and far. This review attempts to give a clear picture on the available literature that describes the osteogenic role of CQ in improving bone health and healing fractures in general and maxillofacial fractures in particular. Also, the role of CQ in green synthesis of bone scaffolds and its osteogenic potential in regeneration of bone using scaffolds are also discussed.

Enhancing the wound healing potential using earthworm clitellum factors and elucidating its molecular mechanism in an in-vitro and earthworm model

Earthworm, Eudrilus eugeniae cannot survive and regenerate without clitellum segments. In regenerating worms, the clitellum’s epithelial and circular muscular layers are reduced to one-third, and longitudinal cell layers to half. In C2C12 cells, Clitellum Factors (CF − 5, 25 and 50%) and Regenerative Clitellum Factors (RCF − 5, 25, 50, 75%) ameliorate the cell viability up to 20–28% and 30–38% respectively than the control. In contrast, extracts from body segments negatively influence cell viability up to 80%. In a scratch-wound assay, 25% RCF and 5% CF achieved 99.86% and 81.54% wound closure in 24 h, respectively, compared to 40% in controls. RCF and CF also possess enhanced anti-microbial activity against gram + ve bacteria. Western Blotting reveals that Wnt3a, HoxD3 and VEGF were remarkably upregulated in RCF and CF treated samples and their upregulated stemness property is effectively regulated by p53, TCTP, H2AX, Cleaved Caspase-3 proteins. Immunofluorescence data clearly states that Wnt3a and Caspase-3 signals are more profoundly observed in nuclear over cytoplasm in RCF treated samples and H2AX shows less nuclear signals than CF. In in-vivo earthworm model conditions, RCF remarkably promotes the survivability and wound healing ability by promoting the Wnt3a and VEGF expression together with downregulation of Cox2.